| + |
Cullin4-RBX1-DDB1 | up-regulates activity 
polyubiquitination
|
OPTN |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272209 |
Lys501 |
LQLAVLLkENDAFED |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 32014991 |
DCAF4-mediated ubiquitination of OPTN facilitates the degradation of DBR-exposed SOD1. OPTN is involved in degradation of DBR-exposed SOD1. These data demonstrate that DCAF4-including CRL4 complex mediates OPTN ubiquitination at lysine 501, and this ubiquitination is absent in the ALS-related OPTNmut. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DCAF1 | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272047 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 22570418 |
By screening the known DDB1 interacting proteins, we discovered that VprBP is the substrate recognition subunit that targets Mcm10 for degradation. Hence, these results establish that Cul4-DDB1-VprBP ubiquitin ligase mediates the stress-induced proteolysis of replication factor, Mcm10. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271675 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 18332868 |
VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation. In this study, we provide evidence to show that Merlin is regulated in a Roc1-Cullin4A-DDB1-dependent manner. Following serum stimulation, Merlin is recruited to the E3 ligase complex through a direct interaction with the WD40-containing adaptor protein VprBP. Loading of Merlin to the E3 ubiquitin ligase complex resulted in its polyubiquitination, and consequently its proteasome-mediated degradation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272228 |
|
|
Homo sapiens |
H-MESO-1 Cell |
| pmid |
sentence |
| 25026211 |
CRL4 DCAF1 ubiquitylates and inhibits Lats. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization 
polyubiquitination
|
WIPI2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272302 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 30898011 |
Here we show that WIPI2/ATG18B (WD repeat domain, phosphoinositide interacting 2), an autophagy-related (ATG) protein that plays a critical role in autophagosome biogenesis, is a direct substrate of CUL4-RING ubiquitin ligases (CRL4s) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CUL4A | form complex 
binding
|
Cullin4-RBX1-DDB1 |
0.902 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-234793 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22649780 |
The CUL4 family employs the structurally distinct triple WD40 ²-propeller domain-containing DDB1 adaptor to recruit members of the DDB1CUL4 associated factors (DCAF) family of substrate receptors |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
PROX1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277610 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 36433955 |
Furthermore, the Ser79 phosphorylation of PROX1 by AMPK enhances the recruitment of CUL4-DDB1 ubiquitin ligase to promote PROX1 degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
CDT1 |
0.515 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272810 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14578910 |
We show that radiation-mediated CDT1 proteolysis is independent of ATM and CHK2 and can occur in G1-phase cells. Loss of the COP9-signalosome (CSN) or CUL4-ROC1 complexes completely suppresses CDT1 proteolysis. CDT1 is specifically polyubiquitinated by CUL4 complexes and the interaction between CDT1 and CUL4 is regulated in part by gamma-irradiation. Our study reveals an evolutionarily conserved and uncharacterized G1 checkpoint that induces CDT1 proteolysis by the CUL4-ROC1 ubiquitin E3 ligase and CSN complexes in response to DNA damage. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RBX1 | form complex
binding
|
Cullin4-RBX1-DDB1 |
0.837 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-234799 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22649780 |
The CUL4 family employs the structurally distinct triple WD40 ²-propeller domain-containing DDB1 adaptor to recruit members of the DDB1CUL4 associated factors (DCAF) family of substrate receptors |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DCAF4 | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272211 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 32014991 |
DCAF4-mediated ubiquitination of OPTN facilitates the degradation of DBR-exposed SOD1. OPTN is involved in degradation of DBR-exposed SOD1. These data demonstrate that DCAF4-including CRL4 complex mediates OPTN ubiquitination at lysine 501, and this ubiquitination is absent in the ALS-related OPTNmut. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272308 |
|
|
Homo sapiens |
Colonic Epithelial Cell |
| pmid |
sentence |
| 30945288 |
We found that both CUL4A and CUL4B can form an E3 complex with DNA damage-binding protein 1 (DDB1) and DDB1-CUL4-associated factor 4 (DCAF4). In vitro and in vivo ubiquitination analyses indicate that CRL4DCAF4 E3 ligase specifically directs degradation of ST7 (suppression of tumorigenicity 7). |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
NF2 |
0.327 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271674 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 18332868 |
VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation. In this study, we provide evidence to show that Merlin is regulated in a Roc1-Cullin4A-DDB1-dependent manner. Following serum stimulation, Merlin is recruited to the E3 ligase complex through a direct interaction with the WD40-containing adaptor protein VprBP. Loading of Merlin to the E3 ubiquitin ligase complex resulted in its polyubiquitination, and consequently its proteasome-mediated degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
SDE2 |
0.25 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272809 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 27906959 |
Here, we identify human SDE2 as a new genome surveillance factor regulated by PCNA interaction. The cleaved SDE2 products need to be degraded by the CRL4CDT2 ubiquitin E3 ligase in a cell cycle- and DNA damage-dependent manner, and failure to degrade SDE2 impairs S phase progression and cellular survival. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
MYC |
0.376 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271965 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20551172 |
We characterize a new Myc-interacting factor, TRPC4AP (transient receptor potential cation channel, subfamily C, member 4-associated protein)/TRUSS (tumor necrosis factor receptor-associated ubiquitous scaffolding and signaling protein), which is the receptor for a DDB1 (damage-specific DNA-binding protein 1)-CUL4 (Cullin 4) E3 ligase complex for selective Myc degradation through the proteasome. TRPC4AP/TRUSS binds specifically to the Myc C terminus and promotes its ubiquitination and destruction through the recognition of evolutionarily conserved domains in the Myc N terminus. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DDB1 | form complex
binding
|
Cullin4-RBX1-DDB1 |
0.919 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-234802 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22649780 |
The CUL4 family employs the structurally distinct triple WD40 ²-propeller domain-containing DDB1 adaptor to recruit members of the DDB1CUL4 associated factors (DCAF) family of substrate receptors |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DCAF11 | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.61 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272262 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 31409866 |
(WDR23) isoforms differentially ubiquitinate (GEN1). In the presence of the necessary components for a successful ubiquitination reaction (CUL4A-DDB1-WDR23 complex, UBE1, UBE2D1, ubiquitin, and ATP) GEN1 receives the addition of ubiquitin in a time dependent manner. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
ST7 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272309 |
|
|
Homo sapiens |
Colonic Epithelial Cell |
| pmid |
sentence |
| 30945288 |
We found that both CUL4A and CUL4B can form an E3 complex with DNA damage-binding protein 1 (DDB1) and DDB1-CUL4-associated factor 4 (DCAF4). In vitro and in vivo ubiquitination analyses indicate that CRL4DCAF4 E3 ligase specifically directs degradation of ST7 (suppression of tumorigenicity 7). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
PYGO2 |
0.297 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273501 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 26170450 |
Here we report that Pygo2 protein is degraded through the ubiquitin/proteasome pathway and is posttranslationally stabilized through phosphorylation by activated phosphatidylinositol 3-kinase/Akt signaling. Specifically, Pygo2 is stabilized upon inhibition of the proteasome, and its intracellular level is regulated by Cullin 4 (Cul4) and DNA damage-binding protein 1 (DDB1), components of the Cul4-DDB1 E3 ubiquitin ligase complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
ubiquitination
|
LATS2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272230 |
|
|
Homo sapiens |
H-MESO-1 Cell |
| pmid |
sentence |
| 25026211 |
CRL4 DCAF1 ubiquitylates and inhibits Lats. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
ubiquitination
|
LATS1 |
0.362 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272229 |
|
|
Homo sapiens |
H-MESO-1 Cell |
| pmid |
sentence |
| 25026211 |
CRL4 DCAF1 ubiquitylates and inhibits Lats. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CRBN | form complex
binding
|
Cullin4-RBX1-DDB1 |
0.788 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-234805 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22649780 |
The CUL4 family employs the structurally distinct triple WD40 ²-propeller domain-containing DDB1 adaptor to recruit members of the DDB1CUL4 associated factors (DCAF) family of substrate receptors |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DTL | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.693 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272848 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 24962565 |
TDG Is Polyubiquitinated by CRL4Cdt2 E3 Ubiquitin Ligase in a PIP Degron-dependent Manner |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
GEN1 |
0.287 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272263 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 31409866 |
(WDR23) isoforms differentially ubiquitinate (GEN1). In the presence of the necessary components for a successful ubiquitination reaction (CUL4A-DDB1-WDR23 complex, UBE1, UBE2D1, ubiquitin, and ATP) GEN1 receives the addition of ubiquitin in a time dependent manner. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
MCM10 |
0.384 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272045 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 22570418 |
By screening the known DDB1 interacting proteins, we discovered that VprBP is the substrate recognition subunit that targets Mcm10 for degradation. Hence, these results establish that Cul4-DDB1-VprBP ubiquitin ligase mediates the stress-induced proteolysis of replication factor, Mcm10. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRPC4AP | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.508 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271964 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20551172 |
We characterize a new Myc-interacting factor, TRPC4AP (transient receptor potential cation channel, subfamily C, member 4-associated protein)/TRUSS (tumor necrosis factor receptor-associated ubiquitous scaffolding and signaling protein), which is the receptor for a DDB1 (damage-specific DNA-binding protein 1)-CUL4 (Cullin 4) E3 ligase complex for selective Myc degradation through the proteasome. TRPC4AP/TRUSS binds specifically to the Myc C terminus and promotes its ubiquitination and destruction through the recognition of evolutionarily conserved domains in the Myc N terminus. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
TDG |
0.296 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272849 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 24962565 |
TDG Is Polyubiquitinated by CRL4Cdt2 E3 Ubiquitin Ligase in a PIP Degron-dependent Manner |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
ubiquitination
|
CSNK1A1 |
0.386 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236907 |
|
|
Homo sapiens |
KG-1 Cell |
| pmid |
sentence |
| 26131937 |
We demonstrate that lenalidomide induces the ubiquitination of casein kinase 1A1 (CK1a) by the E3 ubiquitin ligase CUL4RBX1DDB1CRBN (known as CRL4CRBN) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
MAGEA6 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272266 |
|
|
Homo sapiens |
A-375 Cell |
| pmid |
sentence |
| 31267705 |
The CRL4‐DCAF12 E3 ubiquitin ligase degrades MAGE‐A3/6 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DTL | up-regulates
binding
|
Cullin4-RBX1-DDB1 |
0.693 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272808 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 27906959 |
Here, we identify human SDE2 as a new genome surveillance factor regulated by PCNA interaction. The cleaved SDE2 products need to be degraded by the CRL4CDT2 ubiquitin E3 ligase in a cell cycle- and DNA damage-dependent manner, and failure to degrade SDE2 impairs S phase progression and cellular survival. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHIP | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.327 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266963 |
|
|
Homo sapiens |
K-562 Cell |
| pmid |
sentence |
| 30018425 |
One member of the CRL4 complex, the WD-40 containing protein RepID (DCAF14/PHIP), selectively binds and activates a group of replication origins. Here we show that RepID recruits the CRL4 complex to chromatin prior to DNA synthesis, thus playing a crucial architectural role in the proper licensing of chromosomes for replication. In the absence of RepID, cells rely on the alternative ubiquitin ligase, SKP2-containing SCF, to progress through the cell cycle. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DCAF12 | up-regulates activity
binding
|
Cullin4-RBX1-DDB1 |
0.456 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272264 |
|
|
Homo sapiens |
A-375 Cell |
| pmid |
sentence |
| 31267705 |
The CRL4‐DCAF12 E3 ubiquitin ligase degrades MAGE‐A3/6 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
MAGEA3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272265 |
|
|
Homo sapiens |
A-375 Cell |
| pmid |
sentence |
| 31267705 |
The CRL4‐DCAF12 E3 ubiquitin ligase degrades MAGE‐A3/6 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
ubiquitination
|
UNG |
0.374 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271905 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20870715 |
Using reconstituted CRL4(DCAF1) and CRL4(DCAF1-Vpr) E3 ubiquitin ligases in vitro reveals that UNG2 ubiquitination (ubiquitylation) is facilitated by Vpr. Co-expression of DCAF1 and Vpr causes down-regulation of UNG2 in a proteasome-dependent manner, with Vpr mutants that are defective in UNG2 or DCAF1 binding abrogating this effect. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Cullin4-RBX1-DDB1 | down-regulates quantity by destabilization
polyubiquitination
|
IKZF1 |
0.37 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272083 |
|
|
Homo sapiens |
MM1-S Cell |
| pmid |
sentence |
| 24292625 |
We found that lenalidomide causes selective ubiquitination and degradation of two lymphoid transcription factors, IKZF1 and IKZF3, by the CRBN-CRL4 ubiquitin ligase. IKZF1 and IKZF3 are essential transcription factors in multiple myeloma. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
Cullin4-RBX1-DDB1
CPX-2759