| + |
STK3/4 | down-regulates activity 
phosphorylation
|
MAP1LC3B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273905 |
Thr50 |
QLPVLDKtKFLVPDH |
in vitro |
|
| pmid |
sentence |
| 31857374 |
LC3B is phosphorylated at Thr-50 within the LDS by serine/threonine kinase (STK) 3 and STK4. Here, we identified LIR motifs in STK3 and atypical protein kinase Cζ (PKCζ) and never in mitosis A (NIMA)-related kinase 9 (NEK9). All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-50 impaired binding of several LIR-containing proteins, such as ATG4B, FYVE, and coiled-coil domain-containing 1 (FYCO1), and autophagy cargo receptors p62/sequestosome 1 (SQSTM1) and neighbor of BRCA1 gene (NBR1). |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
NEK9 | down-regulates activity
phosphorylation
|
MAP1LC3B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273904 |
Thr50 |
QLPVLDKtKFLVPDH |
in vitro |
|
| pmid |
sentence |
| 31857374 |
LC3B is phosphorylated at Thr-50 within the LDS by serine/threonine kinase (STK) 3 and STK4. Here, we identified LIR motifs in STK3 and atypical protein kinase Cζ (PKCζ) and never in mitosis A (NIMA)-related kinase 9 (NEK9). All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-50 impaired binding of several LIR-containing proteins, such as ATG4B, FYVE, and coiled-coil domain-containing 1 (FYCO1), and autophagy cargo receptors p62/sequestosome 1 (SQSTM1) and neighbor of BRCA1 gene (NBR1). |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
PRKCZ | down-regulates activity
phosphorylation
|
MAP1LC3B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273906 |
Thr50 |
QLPVLDKtKFLVPDH |
in vitro |
|
| pmid |
sentence |
| 31857374 |
LC3B is phosphorylated at Thr-50 within the LDS by serine/threonine kinase (STK) 3 and STK4. Here, we identified LIR motifs in STK3 and atypical protein kinase Cζ (PKCζ) and never in mitosis A (NIMA)-related kinase 9 (NEK9). All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-50 impaired binding of several LIR-containing proteins, such as ATG4B, FYVE, and coiled-coil domain-containing 1 (FYCO1), and autophagy cargo receptors p62/sequestosome 1 (SQSTM1) and neighbor of BRCA1 gene (NBR1). |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAP1LC3B | down-regulates
binding
|
NBR1 |
0.574 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-184252 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19250911 |
We performed glutathione s-transferase (gst) pull-down assays using extracts from hek293 cells overexpressing an ha-tagged nbr1(d50r) mutant, which lacks the ability to bind p62 (lamark et al., 2003) (figures s1a and s1b, available online), and gst fusions of six human atg8 homologs: gabarap, gabarapl1, gabarapl2, lc3a, lc3b, and lc3c. Indeed, nbr1 interacted with all these members of the mammalian atg8 protein family. downregulation of either lc3 or gabarap (or both) family members leads to stabilization and p62-dependent aggregation of nbr1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ADNP | up-regulates activity 
binding
|
MAP1LC3B |
0.378 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266759 |
|
|
Rattus norvegicus |
|
| pmid |
sentence |
| 24365867 |
Here, we show for the first time that hippocampal ADNP deficiency paralleled reduced beclin1 expression which, in turn, parallels increased tauopathy and cell death. We now show that ADNP directly interacts with LC3B, implicating the requirement of a healthy ADNP system for the apoptotic/autophagy processes. |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| Tissue: |
Hippocampus |
| + |
MAP1LC3B | up-regulates
binding
|
SQSTM1 |
0.832 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-156356 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17580304 |
Sqstm1/p62 (named a170 in the mouse;hereafter p62) is the first proposed example of such proteins (bj_?_?Rk_?_?Y et al.,2005). It binds polyubiquitinated protein aggregates via its uba domain and interacts with lc3 on the autophagosome/ this interaction is necessary for autophagic degradation of p62-positive cytoplasmic inclusion bodies containing ubiquitinated proteins. We also demonstrate that alis are indistinguishable from p62 inclusion bodies and that p62 is required for their formation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-184255 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19250911 |
Sqstm1/p62 (named a170 in the mouse;hereafter p62) is the first proposed example of such proteins (bj_?_?Rk_?_?Y et al.,2005). It binds polyubiquitinated protein aggregates via its uba domain and interacts with lc3 on the autophagosome/ this interaction is necessary for autophagic degradation of p62-positive cytoplasmic inclusion bodies containing ubiquitinated proteins. We also demonstrate that alis are indistinguishable from p62 inclusion bodies and that p62 is required for their formation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
TP53INP1 | up-regulates
binding
|
MAP1LC3B |
0.301 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-196673 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22421968 |
Tp53inp1-lc3 interaction occurs via a functional lc3-interacting region (lir) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ATG7 | up-regulates
binding
|
MAP1LC3B |
0.869 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195239 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22170151 |
These results indicated that the fap motif of atg7 is indispensable for formation of the atg3-lc3 e2-substrate intermediate through the interaction of atg7 with atg3. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRAF6 | down-regulates quantity by destabilization
polyubiquitination
|
MAP1LC3B |
0.3 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277439 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 30806153 |
TRAF6 catalyzes K63-linked polyubiquitination of LC3B and promotes the formation of the LC3B-ATG7 and LC3B-CTNNB1 complexes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAP1LC3B | up-regulates 
|
Autophagosome_formation |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-219403 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20921139 |
We assessed both conversion of LC3-I to its cleaved and lipidated form LC3-II and its translocation to autophagic structures, two steps in autophagosome formation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GDNF | up-regulates quantity by expression
transcriptional regulation
|
MAP1LC3B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252176 |
|
|
Rattus norvegicus |
Neural Stem Cell |
| pmid |
sentence |
| 15212950 |
We characterize the network of 43 genes induced by GDNF overproduction of neuronal progenitor cells (ST14A), which mainly regulate migration and differentiation of neuronal progenitor cells.Laminin, Mpl3, Alcam, Bin1, Id1, Id2, Id3, neuregulin1, the ephrinB2-receptor, neuritin, focal adhesion kinase (FAK), Tc10, Pdpk1, clusterin, GTP-cyclooxygenase1, and follistatin are genes up-regulated by GDNF overexpression. |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| + |
MAP1LC3B | up-regulates
binding
|
ATG3 |
0.828 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-191549 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22170151 |
Lc3-i is activated by the same atg7 involved in atg12 conjugation, transferred to atg3, a second e2-like enzyme, and finally conjugated to pe. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FYCO1 | up-regulates activity
binding
|
MAP1LC3B |
0.532 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260597 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 26468287 |
The preferential binding to LC3A and -B was confirmed in vivo by co-immunoprecipitation experiments of Myc-tagged FYCO1 and GFP fusions of human ATG8 family pro-teins expressed in HEK293 cells (Fig. 2B). GFP-LC3A and GFP-LC3B were efficiently co-precipitated with Myc-FYCO1,whereas GFP-LC3C, GFP-GABARAP, GFP-GABARAPL1 and-L2 were not. The effects we see on late steps of basal autophagy on mutation of the FYCO1 LIR motif correlate with a role of FYCO1 in regulating kinesin-mediated transport of LC3-positive autophagic structures. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
MAP1LC3B |
0.401 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276559 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
ACSS2 | up-regulates quantity by expression
transcriptional regulation
|
MAP1LC3B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276562 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
ATG4B | up-regulates
cleavage
|
MAP1LC3B |
0.796 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-125449 |
|
|
Homo sapiens |
HEK-293 Cell, HeLa Cell |
| pmid |
sentence |
| 15187094 |
Human atg4 homologues cleave the carboxyl termini of the three human atg8 homologues, microtubule-associated protein light chain 3 (lc3), gabarap, and gate-16. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
MAP1LC3B
- 
- 