| + |
STK3/4 | down-regulates activity 
phosphorylation
|
MAP1LC3B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273905 |
Thr50 |
QLPVLDKtKFLVPDH |
in vitro |
|
| pmid |
sentence |
| 31857374 |
LC3B is phosphorylated at Thr-50 within the LDS by serine/threonine kinase (STK) 3 and STK4. Here, we identified LIR motifs in STK3 and atypical protein kinase Cζ (PKCζ) and never in mitosis A (NIMA)-related kinase 9 (NEK9). All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-50 impaired binding of several LIR-containing proteins, such as ATG4B, FYVE, and coiled-coil domain-containing 1 (FYCO1), and autophagy cargo receptors p62/sequestosome 1 (SQSTM1) and neighbor of BRCA1 gene (NBR1). |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SAV1 | up-regulates activity 
binding
|
STK3/4 |
0.889 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263661 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19212654 |
The human WW45 protein enhances MST1-mediated apoptosis in vivo In this model, hWW45 binds both MST1 and LATS through its coiled-coil domains and WW domains, respectively, and facilitates the phosphorylation of LATS by MST1. This model could explain the enhanced apoptotic efficacy of MST1 with the over-expression of hWW45 and the attenuated MST1-induced apoptosis with the down-regulation of endogenous hWW45. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256183 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21084559 |
Mst is activated by binding of salvador (sav1, sav in drosophila), which is, in turn, also phosphorylated by mst. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
PPP2CA | down-regulates
dephosphorylation
|
STK3/4 |
0.73 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269940 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
Rassf1a apparently protects mst1/2 against inactivation by pp2a, the phosphatases that dephosphorylate the stimulatory thr-183 and thr-180 of mst1 andmst2, respectively. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RASSF1 | up-regulates
binding
|
STK3/4 |
0.798 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269950 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21808241 |
Mst1/2 is also activated by binding to Ras association domain family (RASSF) proteins, possibly owing to alteration of Mst1/2 subcellular localization. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
okadaic acid | up-regulates 
|
STK3/4 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269947 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23493077 |
Okadaic acid has been frequently used to enhance the phosphorylation of mst1 and mst2 and to trigger the activation of the hippo pathway. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAF1 | down-regulates
binding
|
STK3/4 |
0.366 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269942 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15618521 |
Raf-1 prevents dimerization and phosphorylation of the activation loop of mst2 independently of its protein kinase activity.Raf-1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (mst2) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STK3/4 | up-regulates activity
phosphorylation
|
SAV1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256184 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21084559 |
Mst is activated by binding of salvador (sav1, sav in drosophila), which is, in turn, also phosphorylated by mst. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
STK3/4 | up-regulates
phosphorylation
|
LATS1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270217 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 15688006 |
Since the N-terminal half of Lats1 (residues 1–588) was dispensable for the activation of Lats1 by Mst2, mass spectrometry was used to identify phosphorylation sites within the C-terminal domain of Lats1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
STK3/4 | up-regulates
phosphorylation
|
MOB1B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270221 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21808241 |
Mob1, which forms a complex with lats1/2, is also phosphorylated by mst1/2, resulting in an enhanced lats1/2 mob1 interaction. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
NF2 | up-regulates activity
binding
|
STK3/4 |
0.427 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269948 |
|
|
Mus musculus |
Cardiomyocyte Cell Line |
| pmid |
sentence |
| 27402866 |
NF2 is activated by oxidative stress in cardiomyocytes and mouse myocardium and facilitates apoptosis. NF2 promotes I/R injury through activation of Mst1 and inhibition of Yap, thereby regulating Hippo signaling in the adult heart. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Hippo Signaling |
| + |
Cell-Cell_contact | up-regulates
|
STK3/4 |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269943 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22683405 |
In response to growth inhibitory signal (e.g. cell–cell contact), MST1/2 in active form phosphorylates LATS1/2 that sequentially phosphorylates YAP at Ser-127. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
TAOK1 | up-regulates
phosphorylation
|
STK3/4 |
0.378 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269938 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
In addition, the thousand-and-one (tao) amino acids kinase or taok13 has been shown to directly phosphorylate and activate hpo or mst1/2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STK3/4 | up-regulates
phosphorylation
|
MOB1A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270220 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 18362890 |
These findings indicate that the phosphorylation of mob1 at thr74 by mst2 is essential to make a complex of mob1, mst2 and ndr1, and to fully activate ndr1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
TAOK2 | up-regulates
phosphorylation
|
STK3/4 |
0.283 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269939 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
In addition, the thousand-and-one (tao) amino acids kinase or taok13 has been shown to directly phosphorylate and activate hpo or mst1/2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TAOK3 | up-regulates
phosphorylation
|
STK3/4 |
0.295 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269949 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
In addition, the thousand-and-one (tao) amino acids kinase or taok1 3 has been shown to directly phosphorylate and activate hpo or mst1/2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ABL1 | up-regulates
phosphorylation
|
STK3/4 |
0.348 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269946 |
|
|
Homo sapiens |
Neuron |
| pmid |
sentence |
| 22590567 |
We demonstrate that c-abl kinase phosphorylates mst2 at an evolutionarily conserved site, y81, within the kinase domain. We further show that the phosphorylation of mst2 by c-abl leads to the disruption of the interaction with raf-1 proteins and the enhancement of homodimerization of mst2 proteins. It thereby enhances the mst2 activation and induces neuronal cell death. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
WWC1 | up-regulates activity
|
STK3/4 |
0.46 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269951 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20159598 |
These results shed light on the mechanism of Ex and Mer function and implicate Kibra as a potential tumor suppressor with relevance to neurofibromatosis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
AKT1 | down-regulates
phosphorylation
|
STK3/4 |
0.408 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269945 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20086174 |
We determined that mst2 phosphorylation by akt limits mst2 activity in two ways: first, by blocking its binding to rassf1a and by promoting its association into the raf-1 inhibitory complex, and second, by preventing homodimerization of mst2, which is needed for its activation. we identified t117 and t384 as akt phosphorylation sites in mst2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STK3/4 | down-regulates
phosphorylation
|
ABL1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270219 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18794806 |
Here, we identify clk1, clk4, mst1, mst2 and ttk (also known as mps1) as novel thr735 kinases in vitro / phosphorylation of thr735 in c-abl is critical for binding to 14-3-3 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STK3/4 | up-regulates activity
phosphorylation
|
Mob1 |
0.895 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256185 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
Mob1, when phosphorylated by MST1/2, binds to the autoinhibitory motif in Lats1/2, which in turn leads to the phosphorylation of the Lats activation loop (Lats1 S909 and Lats2 S872) and thereby an increase of their kinase activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo in cancer, Hippo Signaling |
| + |
AKT3 | down-regulates
phosphorylation
|
STK3/4 |
0.276 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269941 |
|
|
Homo sapiens |
Breast Cancer Cell |
| pmid |
sentence |
| 20231902 |
Akt phosphorylates mst2 at thr117 in vitro and in vivo, which leads to mst2 cleavage and kinase activity as well as nuclear translocation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TAOK | up-regulates activity
phosphorylation
|
STK3/4 |
0.378 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256182 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
The thousand-and-one (TAO) amino acids kinase or TAOK1 – 3 has been shown to directly phosphorylate and activate Hpo or MST1/2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo in cancer, Hippo Signaling |
| + |
AKT2 | down-regulates
phosphorylation
|
STK3/4 |
0.266 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269937 |
|
|
Homo sapiens |
Breast Cancer Cell |
| pmid |
sentence |
| 20231902 |
Akt phosphorylates mst2 at thr117 in vitro and in vivo, which leads to mst2 cleavage and kinase activity as well as nuclear translocation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKT | down-regulates
phosphorylation
|
STK3/4 |
0.408 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269944 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20086174 |
We determined that mst2 phosphorylation by akt limits mst2 activity in two ways: first, by blocking its binding to rassf1a and by promoting its association into the raf-1 inhibitory complex, and second, by preventing homodimerization of mst2, which is needed for its activation. we identified t117 and t384 as akt phosphorylation sites in mst2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STK3/4 | up-regulates
phosphorylation
|
LATS2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270218 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21808241 |
Activation of mst1/2 leads to phosphorylation and activation of their direct substrates, lats1/2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
3.0
STK3/4