| + |
MTOR | down-regulates activity 
phosphorylation
|
TFEB |
0.492 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255310 |
Ser142 |
AGNSAPNsPMAMLHI |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22343943 |
Here, we have used an mTORC1 in-vitro kinase assay and a phosphoantibody to demonstrate that serine S142, which we previously found to be phosphorylated by ERK2, is also phosphorylated by mTOR and that this phosphorylation has a crucial role in controlling TFEB subcellular localization and activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248270 |
Ser211 |
LVGVTSSsCPADLTQ |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 22692423 |
Our data points to the lysosome as the site where mTORC1-dependent phosphorylation of TFEB occurs. [...]Our study has revealed a specific role for phosphorylation of TFEB S211 in the negative regulation of the nuclear abundance of TFEB. This occurs through the promotion of 14-3-3 binding and the masking of the nearby NLS on TFEB. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | MTOR Signaling |
| + |
PPP3CB | up-regulates activity 
dephosphorylation
|
TFEB |
0.384 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255307 |
Ser142 |
AGNSAPNsPMAMLHI |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 26000950 |
Lysosomal Ca2+ release via mucolipin 1 (MCOLN1) activates calcineurin, which binds and de-phosphorylates TFEB, thus promoting its nuclear translocation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255306 |
Ser211 |
LVGVTSSsCPADLTQ |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 26000950 |
Lysosomal Ca2+ release via mucolipin 1 (MCOLN1) activates calcineurin, which binds and de-phosphorylates TFEB, thus promoting its nuclear translocation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
mTORC1 | down-regulates activity
phosphorylation
|
TFEB |
0.371 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255309 |
Ser142 |
AGNSAPNsPMAMLHI |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22343943 |
Here, we have used an mTORC1 in-vitro kinase assay and a phosphoantibody to demonstrate that serine S142, which we previously found to be phosphorylated by ERK2, is also phosphorylated by mTOR and that this phosphorylation has a crucial role in controlling TFEB subcellular localization and activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248274 |
Ser211 |
LVGVTSSsCPADLTQ |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 22692423 |
Our data points to the lysosome as the site where mTORC1-dependent phosphorylation of TFEB occurs. [...]Our study has revealed a specific role for phosphorylation of TFEB S211 in the negative regulation of the nuclear abundance of TFEB. This occurs through the promotion of 14-3-3 binding and the masking of the nearby NLS on TFEB. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
MAPK1 | down-regulates activity
phosphorylation
|
TFEB |
0.425 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248279 |
Ser142 |
AGNSAPNsPMAMLHI |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 21617040 |
Evidence for ERK2-mediated TFEB phosphorylation came from ERK2-TFEB coimmuno-precipitation (fig. S12C) in normal but not in starved medium and from a peptide-based kinase assay showing that mutation of Ser142 to alanine abolished ERK2-mediated phosphorylation ( |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PRKCB | up-regulates activity
phosphorylation
|
TFEB |
0.337 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255315 |
Ser466 |
SKASSRRsSFSMEEG |
Mus musculus |
|
| pmid |
sentence |
| 23599343 |
This occurs following PKCβ phosphorylation of TFEB on three serine residues located in its last 15 amino acids. This post-translational modification stabilizes and increases the activity of this transcription factor. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255316 |
Ser467 |
KASSRRSsFSMEEGD |
Mus musculus |
Osteoclast |
| pmid |
sentence |
| 23599343 |
This occurs following PKCβ phosphorylation of TFEB on three serine residues located in its last 15 amino acids. This post-translational modification stabilizes and increases the activity of this transcription factor. |
|
| Publications: |
2 |
Organism: |
Mus Musculus |
| + |
TFEB | up-regulates quantity by expression 
transcriptional regulation
|
ATP6V1H |
0.31 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276535 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
WDFY3 |
0.274 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276804 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
BLOC1S3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276683 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NEU1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276544 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CTSA |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276549 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy|A chromatin immunoprecipitation (ChIP) assay with antibodies against TFEB or ACSS2 demonstrated that glucose deprivation results in the binding of TFEB (Figure 3D) and ACSS2 (Figure 3E) to the promoter regions of CTSA, GBA, GUSB, and LAMP1|These results indicated that TFEB and ACSS2 are mutually required for their binding to the promoter regions of lysosomal genes. In line with these findings, glucose deprivation induced mRNA (Figure 3F) and protein (Figure 3G) expression for these lysosomal genes, which was largely abrogated by knockin of ACSS2 mutants |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CTSF |
0.374 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276805 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NAGLU |
0.33 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276543 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
GNS |
0.318 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276540 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276792 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
Up-regulated proteins belonged to classes related to protein catabolism, including lysosomal and autophagic proteins (ATP6V1H, CAT, CTSB, CTSC, CTSL, CTSZ, LANCL2, GNS, and PLIN2), endosome/multivesicular body proteins (AP1G1, CHMP1B, CHMP2B, EEA1, RAB7A, and VPS35), Golgi proteins (COPB1 and GALNT5), and the proteasome (PSMA1-5, PSMB2-6, PSMC2-5, PSMD2, PMSD11, and PMSD14) |
|
| Publications: |
2 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
MAP1LC3B |
0.401 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276559 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
ANKFY1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276782 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
APOA4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276783 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CALCOCO1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276784 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
DRAM1 |
0.282 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276788 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
TBK1 |
0.293 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276802 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
TPP1 |
0.293 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276548 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
IL6R |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276798 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
BLOC1S2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276682 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PPM1D |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276557 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NDUFAF2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276703 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
GBA |
0.332 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276551 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy|A chromatin immunoprecipitation (ChIP) assay with antibodies against TFEB or ACSS2 demonstrated that glucose deprivation results in the binding of TFEB (Figure 3D) and ACSS2 (Figure 3E) to the promoter regions of CTSA, GBA, GUSB, and LAMP1|These results indicated that TFEB and ACSS2 are mutually required for their binding to the promoter regions of lysosomal genes. In line with these findings, glucose deprivation induced mRNA (Figure 3F) and protein (Figure 3G) expression for these lysosomal genes, which was largely abrogated by knockin of ACSS2 mutants |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PPARA |
0.281 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276706 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Notably, TFEB regulates genes involved in several steps of lipid catabolism, which occur in different cellular compartments, such as the transport of fatty acid chains across the plasma membrane (for example, Cd36 and Fabps), and the β-oxidation of FFA in mitochondria (for example, Cpt1, Crat, Acadl, Acads and Hdad) and in peroxisomes (Cyp4a genes). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CD36 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276785 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
SGSH |
0.32 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276546 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
ACACB |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276780 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PPP1R15A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276789 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
We found that the main regulator of the starvation-induced transcriptional program, TFEB, counteracts protein synthesis inhibition by directly activating expression of GADD34, a component of the protein phosphatase 1 complex that dephosphorylates eIF2α. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
GOLPH3L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276793 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
Up-regulated proteins belonged to classes related to protein catabolism, including lysosomal and autophagic proteins (ATP6V1H, CAT, CTSB, CTSC, CTSL, CTSZ, LANCL2, GNS, and PLIN2), endosome/multivesicular body proteins (AP1G1, CHMP1B, CHMP2B, EEA1, RAB7A, and VPS35), Golgi proteins (COPB1 and GALNT5), and the proteasome (PSMA1-5, PSMB2-6, PSMC2-5, PSMD2, PMSD11, and PMSD14) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
ACOT2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276781 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
IL1B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276796 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
Up-regulated proteins belonged to classes related to protein catabolism, including lysosomal and autophagic proteins (ATP6V1H, CAT, CTSB, CTSC, CTSL, CTSZ, LANCL2, GNS, and PLIN2), endosome/multivesicular body proteins (AP1G1, CHMP1B, CHMP2B, EEA1, RAB7A, and VPS35), Golgi proteins (COPB1 and GALNT5), and the proteasome (PSMA1-5, PSMB2-6, PSMC2-5, PSMD2, PMSD11, and PMSD14) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
HPS4 |
0.296 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276795 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
Up-regulated proteins belonged to classes related to protein catabolism, including lysosomal and autophagic proteins (ATP6V1H, CAT, CTSB, CTSC, CTSL, CTSZ, LANCL2, GNS, and PLIN2), endosome/multivesicular body proteins (AP1G1, CHMP1B, CHMP2B, EEA1, RAB7A, and VPS35), Golgi proteins (COPB1 and GALNT5), and the proteasome (PSMA1-5, PSMB2-6, PSMC2-5, PSMD2, PMSD11, and PMSD14) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
SACM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276801 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PSAP |
0.314 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276545 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
BECN1 |
0.421 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276558 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
LAMP1 |
0.454 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276555 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy|A chromatin immunoprecipitation (ChIP) assay with antibodies against TFEB or ACSS2 demonstrated that glucose deprivation results in the binding of TFEB (Figure 3D) and ACSS2 (Figure 3E) to the promoter regions of CTSA, GBA, GUSB, and LAMP1|These results indicated that TFEB and ACSS2 are mutually required for their binding to the promoter regions of lysosomal genes. In line with these findings, glucose deprivation induced mRNA (Figure 3F) and protein (Figure 3G) expression for these lysosomal genes, which was largely abrogated by knockin of ACSS2 mutants |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
GUSB |
0.252 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276553 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy|A chromatin immunoprecipitation (ChIP) assay with antibodies against TFEB or ACSS2 demonstrated that glucose deprivation results in the binding of TFEB (Figure 3D) and ACSS2 (Figure 3E) to the promoter regions of CTSA, GBA, GUSB, and LAMP1|These results indicated that TFEB and ACSS2 are mutually required for their binding to the promoter regions of lysosomal genes. In line with these findings, glucose deprivation induced mRNA (Figure 3F) and protein (Figure 3G) expression for these lysosomal genes, which was largely abrogated by knockin of ACSS2 mutants |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CTSD |
0.445 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276537 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
MOGAT2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276799 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PPARGC1A |
0.391 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276708 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Here we show that the transcription factor EB (TFEB), a master regulator of lysosomal biogenesis and autophagy, is induced by starvation through an autoregulatory feedback loop and exerts a global transcriptional control on lipid catabolism via Ppargc1α and Ppar1α. |
|
| Publications: |
1 |
| Pathways: | MTOR Signaling |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
HPS3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276794 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
Up-regulated proteins belonged to classes related to protein catabolism, including lysosomal and autophagic proteins (ATP6V1H, CAT, CTSB, CTSC, CTSL, CTSZ, LANCL2, GNS, and PLIN2), endosome/multivesicular body proteins (AP1G1, CHMP1B, CHMP2B, EEA1, RAB7A, and VPS35), Golgi proteins (COPB1 and GALNT5), and the proteasome (PSMA1-5, PSMB2-6, PSMC2-5, PSMD2, PMSD11, and PMSD14) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
IL24 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276797 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
Up-regulated proteins belonged to classes related to protein catabolism, including lysosomal and autophagic proteins (ATP6V1H, CAT, CTSB, CTSC, CTSL, CTSZ, LANCL2, GNS, and PLIN2), endosome/multivesicular body proteins (AP1G1, CHMP1B, CHMP2B, EEA1, RAB7A, and VPS35), Golgi proteins (COPB1 and GALNT5), and the proteasome (PSMA1-5, PSMB2-6, PSMC2-5, PSMD2, PMSD11, and PMSD14) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NDUFA13 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276701 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NDUFS4 |
0.248 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276705 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PPARG |
0.287 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276707 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Notably, TFEB regulates genes involved in several steps of lipid catabolism, which occur in different cellular compartments, such as the transport of fatty acid chains across the plasma membrane (for example, Cd36 and Fabps), and the β-oxidation of FFA in mitochondria (for example, Cpt1, Crat, Acadl, Acads and Hdad) and in peroxisomes (Cyp4a genes). |
|
| Publications: |
1 |
| Pathways: | MTOR Signaling |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
FABP3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276790 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
On the contrary, proteins increasing the most included those degraded by autophagy (e.g., SQSTM1/p62 and GABARAPL2 |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NDUFA1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276700 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
HEXA |
0.312 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276541 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
LAMP1 |
0.454 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276542 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
PIP4P1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276547 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NDUFB10 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276704 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CYP17A1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276786 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
FGF21 |
0.278 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276791 |
|
|
|
|
| pmid |
sentence |
| 32978159 |
On the contrary, proteins increasing the most included those degraded by autophagy (e.g., SQSTM1/p62 and GABARAPL2 |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
MTM1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276800 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CYP7A1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276787 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Among the differentially expressed genes, we detected upregulation of known targets in TFEB-WT and TFEB-nuc cells (Figure 2B; Tables S1 and S2), including genes functioning in lysosomal and autophagy pathways|Using quantitative PCR (qPCR), we validated expression patterns observed by RNA sequencing for selected genes (CTSD, SQSTM1, MCOLN1, IL33, FAP, GPNMB, IFI30, FOLR1, and G0S2) |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
ATP6V0E1 |
0.316 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276534 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
NDUFA8 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276702 |
|
|
|
|
| pmid |
sentence |
| 33176151 |
Genes responsive to high, sustained levels of nuclear TFEB induced by Torin treatment included CTSF, NPC2, BLOC1S3, and BLOC1S2, which function in lysosomal degradation, transport, and biogenesis; NDUFS4, NDUFA13, NDUFA8, NDUFA1, NDUFB10, and NDUFAF2, subunits of mitochondrial NADH dehydrogenase; PPARG and PPARGC1A, a nuclear receptor and co-factor regulating lipid metabolism; and BHLHE40 and BHLHE41, two transcriptional repressors (Figures 4B and 4D; Table S4). |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
CLCN7 |
0.335 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276536 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
GLA |
0.29 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276539 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
GALNS |
0.293 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276538 |
|
|
|
|
| pmid |
sentence |
| 19556463 |
Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes.|Expression analysis of lysosomal genes after TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- versus pcDNA3-transfected cells. |
|
| Publications: |
1 |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
WDFY1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276803 |
|
|
|
|
| pmid |
sentence |
| 30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
| Publications: |
1 |
3.0
TFEB
- 
- 