| + |
KLK3 | down-regulates activity 
cleavage
|
PTHLH |
0.432 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270546 |
Pro21 |
FLLSYAVpSCGRSVE |
in vitro |
|
| pmid |
sentence |
| 8753751 |
Our study demonstrates that PTHrP is a substrate for PSA. The cleavage of the amino-terminal portion of PTHrP completely disrupts its ability to interact with the PTH/PTHrP receptor and thus inhibits its PTH-like activity. | Our data show that PSA proteolytically cleaves PTHrP (1-34) after either residue 22 or 23, generating three peptide fragments. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270547 |
Ser22 |
LLSYAVPsCGRSVEG |
in vitro |
|
| pmid |
sentence |
| 8753751 |
Our study demonstrates that PTHrP is a substrate for PSA. The cleavage of the amino-terminal portion of PTHrP completely disrupts its ability to interact with the PTH/PTHrP receptor and thus inhibits its PTH-like activity. | Our data show that PSA proteolytically cleaves PTHrP (1-34) after either residue 22 or 23, generating three peptide fragments. |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
PA2G4 | down-regulates quantity by repression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253662 |
|
|
|
|
| pmid |
sentence |
| 16254079 |
Ectopic expression of ebp1, a member of the PA2G4 family, inhibits the proliferation and induces the differentiation of human breast and prostate cancer cell lines. Ebp1 inhibits transcription of E2F1 and androgen receptor regulated genes such as prostate specific antigen (PSA) through its interactions with histone deacetylases (HDACs) |
|
| Publications: |
1 |
| + |
RREB1 | down-regulates quantity by repression 
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253661 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 17550981 |
RREB-1 bound to the prostate-specific antigen (PSA) promoter as assessed by chromatin immunoprecipitation. Transient expression of RREB-1 down-regulated AR-mediated promoter activity and suppressed expression of PSA protein. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SRCAP | up-regulates quantity by expression 
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255220 |
|
|
Homo sapiens |
PrEC Cell |
| pmid |
sentence |
| 20432434 |
ShRNA mediated knockdown of SRCAP resulted in decreased H2A.Z binding at the enhancer region of the PSA promoter and decreased expression of PSA in prostate cancer cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SIN3A | down-regulates quantity by repression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253663 |
|
|
|
|
| pmid |
sentence |
| 16254079 |
Chromatin immunoprecipitation (ChIP) and DNA affinity precipitation analysis demonstrated that Ebp1 and Sin3A associate at the PSA and E2F1 promoters. Functionally, Sin3A enhanced the ability of Ebp1 to repress transcription of androgen receptor (AR) and E2F1 regulated genes. |
|
| Publications: |
1 |
| + |
AR | up-regulates quantity by expression 
transcriptional regulation
|
KLK3 |
0.816 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253657 |
|
|
|
|
| pmid |
sentence |
| 20069563 |
TH1 also associates with AR at the active androgen-responsive prostate-specific antigen (PSA) promoter in the nucleus of LNCaP cells. Decrease of endogenous AR protein by TH1 interferes with androgen-induced luciferase reporter expression and reduces endogenous PSA expression. |
|
| Publications: |
1 |
| + |
BTG2 | down-regulates quantity by repression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253658 |
|
|
|
|
| pmid |
sentence |
| 21172304 |
Androgen-induced promoter activation and expression of prostate-specific antigen (PSA) are significantly attenuated by BTG2. |
|
| Publications: |
1 |
| + |
AATF | up-regulates quantity by expression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253669 |
|
|
|
|
| pmid |
sentence |
| 23146908 |
Chromatin immunoprecipitation in combination with siRNA-mediated knockdown revealed that recruitment of AATF and ZIPK to the PSA enhancer was dependent on AR, whereas recruitment of TSG101 was dependent on AATF. |
|
| Publications: |
1 |
| + |
KLK3 | up-regulates activity
binding
|
PTHLH |
0.432 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270548 |
|
|
in vitro |
|
| pmid |
sentence |
| 8683730 |
Prostate-specific antigen was found to specifically cleave PTHrP 1-141 in a time- and dose-dependent manner.|The preferred PSA cleavage site of PTHrP 1-141 was determined to be at the carboxyl-terminus of phenylalanine 23, consistent with chymotryptic-like enzymatic activity of PSA. Cleavage of PTHrP by PSA completely abolished the ability of PTHrP to stimulate cAMP production. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
NCOA4 | up-regulates quantity by expression
transcriptional regulation
|
KLK3 |
0.334 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253659 |
|
|
Homo sapiens |
PC-J Cell |
| pmid |
sentence |
| 18649734 |
The PSA promoter activity was detected by transient expression assay in the PC-J and LNCaP cells but not in androgen insensitive PC-3 cells. When the PC-J cells were cotransfected with androgen receptor, androgen receptor coactivators and PSA reporter vector cells, the reporter assays indicated that nuclear receptor coactivator 4 (NCOA4) but not androgen receptor activator 24 (ARA24) increased the sensitivity and maximum stimulation of dihydrotestosterone (DHT)-inducing PSA promoter activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NfKb-p65/p50 | up-regulates quantity by expression
transcriptional regulation
|
KLK3 |
0.267 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253667 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 11909978 |
NF-kappa B activates prostate-specific antigen expression and is upregulated in androgen-independent prostate cancer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SP1 | up-regulates quantity by expression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253664 |
|
|
Homo sapiens |
Prostate Cancer Cell Line |
| pmid |
sentence |
| 15708372 |
We characterized four Sp1/Sp3 binding sites in the proximal promoter of the PSA gene. In a luciferase assay, these sites contributed to the basal promoter activity in prostate cancer cells. In an electrophoretic mobility shift assay and chromatin immunoprecipitation assay, we confirmed that Sp1 and Sp3 bind to these sites. Overexpression of wild-type Sp1 and Sp3 further upregulated the promoter activity, whereas overexpression of the Sp1 dominant-negative form or addition of mithramycin A significantly reduced the promoter activity and the endogenous mRNA level of PSA. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FOXP1 | down-regulates quantity by repression
transcriptional regulation
|
KLK3 |
0.278 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253660 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 18640093 |
Notably, we demonstrate that FOXP1 directly interacts with AR and negatively regulates AR signaling ligand-dependently, as exemplified by the transcriptional repression of PSA gene regulated by androgen-dependent FOXP1 recruitment on its enhancer region. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HDAC1 | down-regulates quantity by repression
transcriptional regulation
|
KLK3 |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253666 |
|
|
|
|
| pmid |
sentence |
| 11994312 |
To define a mechanism for repression of AR function, we demonstrate that AR activity is specifically down-regulated by the histone deacetylase activity of HDAC1. Furthermore, using both mammalian two-hybrid and immunoprecipitation experiments, we show that AR and HDAC1 interact, suggestive of a direct role for down-regulation of AR activity by HDAC1. In chromatin immunoprecipitation assays, we provide evidence that AR, Tip60, and HDAC1 form a trimeric complex upon the endogenous AR-responsive PSA promoter, suggesting that acetylation and deacetylation of the AR is an important mechanism for regulating transcriptional activity. |
|
| Publications: |
1 |
| + |
SP3 | up-regulates quantity by expression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253665 |
|
|
Homo sapiens |
Prostate Cancer Cell Line |
| pmid |
sentence |
| 15708372 |
We characterized four Sp1/Sp3 binding sites in the proximal promoter of the PSA gene. In a luciferase assay, these sites contributed to the basal promoter activity in prostate cancer cells. In an electrophoretic mobility shift assay and chromatin immunoprecipitation assay, we confirmed that Sp1 and Sp3 bind to these sites. Overexpression of wild-type Sp1 and Sp3 further upregulated the promoter activity, whereas overexpression of the Sp1 dominant-negative form or addition of mithramycin A significantly reduced the promoter activity and the endogenous mRNA level of PSA. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFKB1 | up-regulates quantity by expression
transcriptional regulation
|
KLK3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253668 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 11909978 |
NF-kappa B activates prostate-specific antigen expression and is upregulated in androgen-independent prostate cancer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
KLK3
- 
- 