| + |
CBP/p300 | up-regulates activity 
acetylation
|
SMAD2 |
0.649 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-235899 |
Lys19 |
VKRLLGWkKSAGGSG |
Homo sapiens |
HeLa Cell, HEK-293T Cell, HaCaT Cell |
| pmid |
sentence |
| 17074756 |
We demonstrate that both smad2 and smad3 are acetylated by the coactivators p300 and cbp in a tgfbeta-dependent manner. To identify the specific lysine residue acetylated by p300, lys19, and lys20 in smad2(fl) were mutated individually and subjected to p300-mediated acetylation following expression in 293t cells. Mutation of lys19 blocked the p300-mediated acetylation of smad2(fl), whereas mutation of lys20 had no effect (fig. 2b), suggesting that lys19 is the preferred site for p300-mediated acetylation of smad2(fl). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | TGF-beta Signaling |
| + |
CBP/p300 | up-regulates activity
acetylation
|
SMAD3 |
0.685 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236126 |
Lys19 |
VKRLLGWkKGEQNGQ |
Homo sapiens |
HeLa Cell, HEK-293T Cell, HaCaT Cell |
| pmid |
sentence |
| 17074756 |
We demonstrate that both smad2 and smad3 are acetylated by the coactivators p300 and cbp in a tgfb-dependent manner. the p300-dependent acetylation of smad3 was attenuated when lys19 was mutated, whereas mutation of lys20 had no effect, suggesting that lys19 is acetylated also in smad3. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-227553 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9865691 |
The closely related CBP and p300 proteins are also important coactivators for Smad activity. CBP and p300 act as coactivators of several transcription factors by bringing the sequence-specific activators within proximity of the general transcription machinery and by modifying the chromatin structure through histone acetylation.In response to TGF-b, Smad3 associates with CBP/p300 and TGF-b-induced C-terminal phosphorylation of Smad3 promotes this association. This association with CBP/p300 is likely to be essential for transcriptional activity of Smad3. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | TGF-beta Signaling |
| + |
CBP/p300 | down-regulates activity 
acetylation
|
H3-3A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217214 |
Lys28 |
LATKAARkSAPSTGG |
Homo sapiens |
|
| pmid |
sentence |
| 21131905 |
These results highlight the substrate and site specificities of hats in cells, demonstrate the distinct roles of gcn5/pcaf- and cbp/p300-mediated histone acetylations in gene activation, and suggest an important role of cbp/p300-mediated h3k18/27ac in nr-dependent transcription. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates quantity by expression 
transcriptional regulation
|
ALOX15 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254100 |
|
|
Homo sapiens |
A-549 Cell |
| pmid |
sentence |
| 12517954 |
IL-4 has been shown to up-regulate 15-lipoxygenase and produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE) in A549 cells via the Janus kinase/STAT6 pathway under coactivation of CREB binding protein/p300. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates activity
acetylation
|
YY1 |
0.641 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268834 |
|
|
in vitro |
|
| pmid |
sentence |
| 11486036 |
Previous studies have established that YY1 interacts with histone acetyltransferases p300 and CREB-binding protein (CBP) and histone deacetylase 1 (HDAC1), HDAC2, and HDAC3. Here, we present evidence that the activity of YY1 is regulated through acetylation by p300 and PCAF and through deacetylation by HDACs. YY1 was acetylated in two regions: both p300 and PCAF acetylated the central glycine-lysine-rich domain of residues 170 to 200, and PCAF also acetylated YY1 at the C-terminal DNA-binding zinc finger domain. Acetylation of the central region was required for the full transcriptional repressor activity of YY1 and targeted YY1 for active deacetylation by HDACs. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
CBP/p300 | up-regulates
binding
|
MEF2C |
0.705 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-83840 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11062529 |
Cbp/p300 and pcaf are coactivators for myod and mef-2c during myogenic commitment and differentiation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F5 | up-regulates activity
binding
|
CBP/p300 |
0.561 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262733 |
|
|
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 10783242 |
Here we show that E2F-5 is phosphorylated by the cyclin E-Cdk2 complex, which functions in the late G1 phase, but not by the early-G1-phase-acting cyclin D-CDK complex. A phosphorylation site in the trans-activation domain of E2F-5 stimulates transcription and cell-cycle progression by the recruitment of the p300/CBP family of co-activators, whose binding to E2F-5 is stabilized upon phosphorylation by cyclin E-Cdk2. These results indicate that phosphorylation of E2F-5 at the CDK site at position 251 by cyclin E–Cdk2 augments transcription by enhancing the interaction of E2F-5 with p300/CBP co-activator proteins. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates
binding
|
DDX5 |
0.47 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-97274 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12527917 |
Cbp/p300 interact with p68 rna helicase / the atpase activity of p68 is required for the specific transcriptional activation of cbp |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TP53 | up-regulates
binding
|
CBP/p300 |
0.91 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-66956 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10207072 |
Both p53 and rela(p65) interact with the transcriptional coactivator proteins p300 and creb-binding protein (cbp), and we demonstrate that these results are consistent with competition for a limiting pool of p300/cbp complexes in vivo. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates quantity by expression
transcriptional regulation
|
EPCAM |
0.256 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254791 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11505407 |
The current results provide the first insights into the regulation of EpCAM expression, which is regulated negatively by TNFalpha and TPA through the activation of NF-kappaB. The repression may rely on the competition of NF-kappaB for p300/CBP histone acetyl transferase activity, because the overexpression of p300 reverts TNFalpha effects. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RELA | up-regulates
binding
|
CBP/p300 |
0.845 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217655 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10207072 |
Both p53 and rela(p65) interact with the transcriptional coactivator proteins p300 and creb-binding protein (cbp), and we demonstrate that these results are consistent with competition for a limiting pool of p300/cbp complexes in vivo. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates activity
acetylation
|
KLF1 |
0.47 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251789 |
|
|
Homo sapiens |
Erythroid Cell |
| pmid |
sentence |
| 9707565 |
CBP and p300, but Not P/CAF, Enhance EKLF Trans-activation in Erythroid Cells. We find that EKLF is an acetylated transcription factor, and that it interacts in vivo with CBP, p300, and P/CAF. However, its interactions with these histone acetyltransferases are not equivalent, as CBP and p300, but not P/CAF, utilize EKLF as a substrate for in vitro acetylation within its trans-activation region. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CREBBP | form complex 
binding
|
CBP/p300 |
0.527 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-110559 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11559745 |
P300/cbp proteins: hats for transcriptional bridges and scaffolds |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | down-regulates
acetylation
|
FLI1 |
0.295 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-172109 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21321929 |
We have previously demonstrated that in response to transforming growth factor _ (tgf_), fli-1 activity is repressed through a series of sequential posttranslational modifications, consisting of protein kinase c_ (pkc_)-induced thr312 phosphorylation, acetylation by p300/creb binding protein-associated factor, and detachment from the collagen promoter. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates
binding, acetylation
|
MYOD1 |
0.636 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-81047 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10944526 |
Our results provide direct evidence that myod acetylation functionally activates the protein and show that both pcaf and cbp/p300 are candidate enzymes for myod acetylation in vivo. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217220 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10944526 |
Our results provide direct evidence that myod acetylation functionally activates the protein and show that both pcaf and cbp/p300 are candidate enzymes for myod acetylation in vivo. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Tissue: |
Muscle |
| + |
CBP/p300 | up-regulates quantity by expression
transcriptional regulation
|
THBD |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253908 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15677570 |
We further show evidence suggesting that NF-κB inhibits TM expression indirectly by competition for the coactivator p300/CBP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates
binding
|
SMAD1 |
0.404 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217217 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12419246 |
Thus, Ski/SnoN represses TGFβ signaling by multiple mechanisms. In addition to recruitment of a transcriptional repressor complex and dissociation of the transcriptional coactivator p300/CBP from the Smads |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | up-regulates
acetylation
|
RELA |
0.845 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217210 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16382138 |
Rela is also acetylated at several sites by p300 and cbp |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MLL2 complex | up-regulates activity
binding
|
CBP/p300 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268814 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28669924 |
KMT2D associates with WRAD (WDR5, RbBP5, ASH2L, and DPY30), NCOA6, PTIP, PA1, and H3K27 demethylase UTX in one protein complex. It acts as a scaffold protein within the complex and is responsible for maintaining the stability of UTX. KMT2D is a major mammalian H3K4 mono-methyltransferase and co-localizes with lineage determining transcription factors on transcriptional enhancers. It is required for the binding of histone H3K27 acetyltransferases CBP and p300 on enhancers, enhancer activation and cell-type specific gene expression during differentiation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
EP300 | form complex
binding
|
CBP/p300 |
0.527 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-110562 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11559745 |
P300/cbp proteins: hats for transcriptional bridges and scaffolds |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SNIP1 | down-regulates
binding
|
CBP/p300 |
0.593 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217661 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10887155 |
In this study, we characterize a novel nuclear protein, termed snip1 its principal mechanism of action appears to be through transcription by binding to cbp/p300 and interfering with the ability of these coactivators to interact with smad4 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CBP/p300 | down-regulates activity
acetylation
|
Histone H3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265322 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21131905 |
These results highlight the substrate and site specificities of hats in cells, demonstrate the distinct roles of gcn5/pcaf- and cbp/p300-mediated histone acetylations in gene activation, and suggest an important role of cbp/p300-mediated h3k18/27ac in nr-dependent transcription. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
CBP/p300