| + |
PRKACA | up-regulates activity 
phosphorylation
|
E2F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277538 |
Ser235 |
TTQLRLLsEDTDSQR |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 33110360 |
We confirmed the phosphorylation of T130, S235, and S364 by developing monoclonal antibodies against phospho-specific forms of these sites and showed that their phosphorylation is cell cycle-dependent. According to our results, PKA-mediated phosphorylation of E2F1 by PKA inhibits proliferation and glucose uptake and induces caspase-3 activation and senescence. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277537 |
Ser364 |
PLLSRMGsLRAPVDE |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 33110360 |
We confirmed the phosphorylation of T130, S235, and S364 by developing monoclonal antibodies against phospho-specific forms of these sites and showed that their phosphorylation is cell cycle-dependent. According to our results, PKA-mediated phosphorylation of E2F1 by PKA inhibits proliferation and glucose uptake and induces caspase-3 activation and senescence. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277536 |
Thr130 |
GEKSRYEtSLNLTTK |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 33110360 |
We confirmed the phosphorylation of T130, S235, and S364 by developing monoclonal antibodies against phospho-specific forms of these sites and showed that their phosphorylation is cell cycle-dependent. According to our results, PKA-mediated phosphorylation of E2F1 by PKA inhibits proliferation and glucose uptake and induces caspase-3 activation and senescence. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
ATR | up-regulates quantity by stabilization
phosphorylation
|
E2F1 |
0.378 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-109420 |
Ser31 |
ALRLLDSsQIVIISA |
Mus musculus |
Thymocyte |
| pmid |
sentence |
| 11459832 |
These results thus suggest that this serine 31 residue is indeed an atm/atr phosphorylation site and in fact is the major site for atm/atr-mediated phosphorylation within e2f1. Thus, it is possible that the atm/atr-mediated phosphorylation inhibits the binding and function of skp2 and thus prevents the normal degradation of e2f1 |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
ATM | up-regulates quantity by stabilization
phosphorylation
|
E2F1 |
0.678 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-109416 |
Ser31 |
ALRLLDSsQIVIISA |
Mus musculus |
|
| pmid |
sentence |
| 11459832 |
Selective induction of e2f1 in response to dna damage, mediated by atm-dependent phosphorylation. We identify a site for atm/atr phosphorylation in the amino terminus of e2f1 and we show that this site is required for atm-mediated stabilization of e2f1. Finally, we also show that e2f1 is required for dna damaged induced apoptosis in mouse thymocytes. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CDK1 | up-regulates
phosphorylation
|
E2F1 |
0.698 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-36022 |
Ser332 |
TDSATIVsPPPSSPP |
Homo sapiens |
|
| pmid |
sentence |
| 8087847 |
Association of e2f with rb inhibits its transactivation potential. phosphorylation of e2f-1 on serine residues 332 and 337 prevented its interaction with rbthese residues were phosphorylated in vivo and by p34cdc2 kinase in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-36026 |
Ser337 |
IVSPPPSsPPSSLTT |
Homo sapiens |
|
| pmid |
sentence |
| 8087847 |
Association of e2f with rb inhibits its transactivation potential. phosphorylation of e2f-1 on serine residues 332 and 337 prevented its interaction with rbthese residues were phosphorylated in vivo and by p34cdc2 kinase in vitro. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CHEK2 | up-regulates activity
phosphorylation
|
E2F1 |
0.49 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260822 |
Ser364 |
PLLSRMGsLRAPVDE |
Homo sapiens |
|
| pmid |
sentence |
| 12717439 |
Therefore, Chk2 phosphorylates and activates E2F-1 in response to DNA damage, resulting in apoptosis. | These results suggest that the Ser 364 site is phosphorylated by Chk2 and that anti-P-Ser 364 recognises the phosphorylated site in E2F-1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CHEK2 | up-regulates quantity by stabilization
phosphorylation
|
E2F1 |
0.49 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-197278 |
Ser364 |
PLLSRMGsLRAPVDE |
Homo sapiens |
|
| pmid |
sentence |
| 22558186 |
Among these effector proteins, chk1 phosphorylates tlk12 and rad51, while brca, pik3, pml and e2f1 are chk2 substrates. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-100898 |
Ser364 |
PLLSRMGsLRAPVDE |
Homo sapiens |
|
| pmid |
sentence |
| 12717439 |
We report that checkpoint kinase 2 (chk2) regulates e2f-1 activity in response to the dna-damaging agent etoposide. A chk2 consensus phosphorylation site in e2f-1 is phosphorylated in response to dna damage |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CKM complex | down-regulates activity 
phosphorylation
|
E2F1 |
0.364 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273149 |
Ser375 |
PVDEDRLsPLVAADS |
Homo sapiens |
|
| pmid |
sentence |
| 18794899 |
E2F1 activity is also repressed by cyclin-dependent kinase-8 (CDK8), a colorectal oncoprotein. Elevated levels of CDK8 protect beta-catenin/TCF-dependent transcription from inhibition by E2F1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273138 |
Ser375 |
PVDEDRLsPLVAADS |
Homo sapiens |
|
| pmid |
sentence |
| 22945643 |
Cdk8 regulates e2f1 transcriptional activity through s375 phosphorylation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDK8 | down-regulates
phosphorylation
|
E2F1 |
0.486 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-181078 |
Ser375 |
PVDEDRLsPLVAADS |
Homo sapiens |
|
| pmid |
sentence |
| 18794899 |
E2F1 activity is also repressed by cyclin-dependent kinase-8 (CDK8), a colorectal oncoprotein. Elevated levels of CDK8 protect beta-catenin/TCF-dependent transcription from inhibition by E2F1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-198934 |
Ser375 |
PVDEDRLsPLVAADS |
Homo sapiens |
|
| pmid |
sentence |
| 22945643 |
Cdk8 regulates e2f1 transcriptional activity through s375 phosphorylation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
TFIIH | down-regulates
phosphorylation
|
E2F1 |
0.319 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269353 |
Ser403 |
PEEFISLsPPHEALD |
Homo sapiens |
|
| pmid |
sentence |
| 10428966 |
These results suggest that tfiih-mediated phosphorylation of e2f-1 plays a role in triggering e2f-1 degradation during s phase. here we show that the e2f-1 activation domain interacts with a kinase activity which phosphorylates two sites, ser403 and thr433, within the activation domain. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269354 |
Thr433 |
DCDFGDLtPLDF |
Homo sapiens |
|
| pmid |
sentence |
| 10428966 |
These results suggest that tfiih-mediated phosphorylation of e2f-1 plays a role in triggering e2f-1 degradation during s phase. here we show that the e2f-1 activation domain interacts with a kinase activity which phosphorylates two sites, ser403 and thr433, within the activation domain. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDK7 | down-regulates
phosphorylation
|
E2F1 |
0.495 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-69776 |
Ser403 |
PEEFISLsPPHEALD |
Homo sapiens |
|
| pmid |
sentence |
| 10428966 |
These results suggest that tfiih-mediated phosphorylation of e2f-1 plays a role in triggering e2f-1 degradation during s phase. here we show that the e2f-1 activation domain interacts with a kinase activity which phosphorylates two sites, ser403 and thr433, within the activation domain. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-69780 |
Thr433 |
DCDFGDLtPLDF |
Homo sapiens |
|
| pmid |
sentence |
| 10428966 |
These results suggest that tfiih-mediated phosphorylation of e2f-1 plays a role in triggering e2f-1 degradation during s phase. here we show that the e2f-1 activation domain interacts with a kinase activity which phosphorylates two sites, ser403 and thr433, within the activation domain. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
GTF2H1 | down-regulates quantity by destabilization
phosphorylation
|
E2F1 |
0.437 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251259 |
Ser403 |
PEEFISLsPPHEALD |
in vitro |
|
| pmid |
sentence |
| 10428966 |
TFIIH-mediated phosphorylation of E2F-1 plays a role in triggering E2F-1 degradation during S phase. E2F-1 activation domain interacts with a kinase activity which phosphorylates two sites, Ser403 and Thr433, within the activation domain. We demonstrate that TFIIH is responsible for the E2F-1 phosphorylation observed in cell extracts and that endogenous E2F-1 interacts in vivo with p62, a component of TFIIH, during S phase. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251260 |
Thr433 |
DCDFGDLtPLDF |
in vitro |
|
| pmid |
sentence |
| 10428966 |
TFIIH-mediated phosphorylation of E2F-1 plays a role in triggering E2F-1 degradation during S phase. E2F-1 activation domain interacts with a kinase activity which phosphorylates two sites, Ser403 and Thr433, within the activation domain. We demonstrate that TFIIH is responsible for the E2F-1 phosphorylation observed in cell extracts and that endogenous E2F-1 interacts in vivo with p62, a component of TFIIH, during S phase. |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
E2F1 | up-regulates quantity by expression 
transcriptional regulation
|
CTNND2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251876 |
|
|
Mus musculus |
Prostate Cancer Cell Line |
| pmid |
sentence |
| 21106062 |
Coordinated regulation of δ-catenin expression by both the activating transcription factor E2F1 and repressive transcription factor Hes1 in prostate cancer progression. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
SCF-SKP2 | down-regulates quantity by destabilization
polyubiquitination
|
E2F1 |
0.383 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272557 |
|
|
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 10559858 |
P45 SKP2 binds to a specific domain of E2F-1. We propose a model in which an SCFSKP2-dependent ubiquitination pathway contributes to the timely ubiquitination and degradation of E2F-1 in the S/G2 phases of the cell cycle. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates
binding
|
HDAC1 |
0.65 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199952 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23213415 |
Furthermore, smad7 caused hdac-1 bind to e2f-1 to form a ternary complex on chromosomal dna containing an e2f-binding motif and leading to repression in the activity of the e2f target genes |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HIC1 | down-regulates quantity by repression 
transcriptional regulation
|
E2F1 |
0.29 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254239 |
|
|
Homo sapiens |
Fibroblast |
| pmid |
sentence |
| 19486893 |
HIC1 is also implicated in growth control since it recruits BRG1, one of the two alternative ATPases (BRM or BRG1) of SWI/SNF chromatin-remodeling complexes to repress transcription of E2F1 in quiescent fibroblasts. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
SERPINB5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254135 |
|
|
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 20197383 |
Importantly, we show that E2F1-mediated upregulation of maspin is enhanced by chemotherapeutic drugs, and inhibition of maspin expression significantly impairs the ability of E2F1 to promote chemotherapy-induced apoptosis. Summarily, our data indicate that maspin is an important effector of E2F1-induced chemosensitization. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
ATM |
0.678 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-198470 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22832221 |
Brca1/e2f1/ctipbinding to atm promoter activates atm transcription. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
ABCB1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253841 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23542036 |
We show here that EAPP stimulates the MDR1 promoter resulting in higher PGP levels. Independently of EAPP, E2F1 also increases the activity of the MDR1 promoter. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
CCNE1 |
0.681 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253854 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245474 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 8649818 |
We have found that cell cycle regulation of cyclin E transcription is mediated by E2F binding sites present in the promoter. The activity of this promoter can be regulated negatively by pRB. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
ELF4 |
0.247 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253849 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20805247 |
we determined that E2F1 specifically binds to MEF promoter and transactivates MEF. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates activity
binding
|
TFDP1 |
0.812 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-240547 |
|
|
Cricetulus griseus |
|
| pmid |
sentence |
| 8832394 |
The transcriptionally active forms of E2F are heterodimers composed of one polypeptide encoded by the E2F gene family and one polypeptide encoded by the DP gene family.In transfected cells, DP-1 did not accumulate in the nucleus unless it was coexpressed with the heterodimeric partners E2F-1, E2F-2, or E2F-3. |
|
| Publications: |
1 |
Organism: |
Cricetulus Griseus |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
PPARG |
0.464 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-90459 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12110166 |
We show here that e2f1 induces ppar gamma transcription during clonal expansion, whereas e2f4 represses pparg amma expression during terminal adipocyte differentiation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
DLK1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271684 |
|
|
|
|
| pmid |
sentence |
| 19874716 |
Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. |
|
| Publications: |
1 |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
CyclinE/CDK2 |
0.57 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245471 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 8649818 |
We have found that cell cycle regulation of cyclin E transcription is mediated by E2F binding sites present in the promoter. The activity of this promoter can be regulated negatively by pRB. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
MYBL2 |
0.497 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253855 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | down-regulates quantity by repression
transcriptional regulation
|
HSPA5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253845 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18840615 |
we show that E2F1 represses GRP78/BIP at the transcriptional level, and this requires its DNA binding domain. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
CDC25A |
0.552 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245468 |
|
|
Homo sapiens |
MCF-7 Cell |
| pmid |
sentence |
| 11154267 |
Expression of Cdc25A is transcriptionally regulated by Myc and E2F-1 , both of which are expressed in MCF-7 cells in response to estrogen |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
E2F1 | up-regulates
transcriptional regulation
|
PPARG |
0.464 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-210047 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12110166 |
We show here that e2f1 induces ppar gamma transcription during clonal expansion, whereas e2f4 represses pparg amma expression during terminal adipocyte differentiation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
ISYNA1 |
0.312 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254130 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15464731 |
Human myo-inositol 1-phosphate synthase (IP synthase; E.C. 5.5.1.4), encoded by ISYNA1, catalyzes the de novo synthesis of inositol 1-phosphate from glucose 6-phosphate.|Here, we have characterized the minimal promoter of ISYNA1 and show that it is upregulated by E2F1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BIRC2 | up-regulates activity
ubiquitination
|
E2F1 |
0.337 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278688 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28542143 |
The ability of cIAP1 to promote wt E2F1 transcriptional activity was retained by all E2F1 mutants, except the K161R/164R mutant for which cIAP1 was completely inactive and the K185R whose basal transactivation activity was weakly enhanced by cIAP1 (XREF_FIG).|Here, we demonstrated that the E3-ubiquitin ligase cellular inhibitor of apoptosis 1 (cIAP1) increases E2F1 K63-poly-ubiquitination on the lysine residue 161/164 cluster, which is associated with the transcriptional factor stability and activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
RASGRP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253850 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18396012 |
We demonstrate that E2F1 induces ERK activation via a transcriptional mechanism and upregulates the expression of two guanine nucleotide exchange factors, RASGRP1 and RASGEF1B, which promote Ras activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
RASGEF1B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253851 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18396012 |
We demonstrate that E2F1 induces ERK activation via a transcriptional mechanism and upregulates the expression of two guanine nucleotide exchange factors, RASGRP1 and RASGEF1B, which promote Ras activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates
|
G1/S_transition |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245477 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21524151 |
In its hypophosphorylated state, pRb binds transcription factors of the E2F family which are required for cell cycle progression. As the level of CyclinD/Cdk4/6 complexes increases, pRb becomes phosphorylated and progression through G1 occurs. At a critical level of phosphorylation, E2F is released from pRb. This activates the transcription of CyclinE which complexes with Cdk2 to fully release pRb repression by further phosphorylation, establishing a positive feedback loop. E2F further promotes the transcription of S-phase genes. Thus, CyclinD/Cdk4/6 and CyclinE/Cdk2 together regulate S-phase entry via phosphorylating pRb, which controls pRb binding to E2F |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Luminal Breast Cancer, Malignant Melanoma, Pancreatic ductal adenocarcinoma (PDA) |
| + |
E2F1 | up-regulates activity
transcriptional regulation
|
CDK1 |
0.698 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253864 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
CD2AP |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254129 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22880102 |
Transcriptional activation of the human CD2AP promoter by E2F1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
TYMS |
0.526 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253863 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | form complex 
binding
|
SMAD7/HDAC1/E2F-1 |
0.45 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199955 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23213415 |
Furthermore, smad7 caused hdac-1 bind to e2f-1 to form a ternary complex on chromosomal dna containing an e2f-binding motif and leading to repression in the activity of the e2f target genes |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPK3 | up-regulates
phosphorylation
|
E2F1 |
0.294 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-121991 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14967450 |
Erk also undergoes rapid translocation into the nucleus, where it phosphorylates and activates a variety of transcription factor targets, including sp1, e2f, elk-1, and ap1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-198292 |
|
|
Homo sapiens |
Breast Cancer Cell |
| pmid |
sentence |
| 22802261 |
Erk also undergoes rapid translocation into the nucleus, where it phosphorylates and activates a variety of transcription factor targets, including sp1, e2f, elk-1, and ap1 |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
RRM1 |
0.301 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253852 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HDAC3 | up-regulates
binding
|
E2F1 |
0.492 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199961 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23213415 |
Furthermore, smad7 caused hdac-1 bind to e2f-1 to form a ternary complex on chromosomal dna containing an e2f-binding motif and leading to repression in the activity of the e2f target genes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NDN | down-regulates activity
binding
|
E2F1 |
0.597 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253382 |
|
|
Mus musculus |
Neocortex |
| pmid |
sentence |
| 24392139 |
Necdin interacts with E2F transcription factors and suppresses E2F1-dependent transcriptional activation of the cyclin-dependent kinase Cdk1 gene. Here we show that necdin serves as a suppressor of NPC proliferation in the embryonic neocortex. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
HES1 | down-regulates quantity by repression
transcriptional regulation
|
E2F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-189061 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19891787 |
We earlier identified e2f-1 as a crucial transcription factor directly inhibited by hes-1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMARCB1 | down-regulates
|
E2F1 |
0.248 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-92788 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12226744 |
We show that the ectopic expression of wild-type hsnf5/ini1, but not that of truncated versions, leads to a cell cycle arrest by inhibiting the entry into s phase of mrt cells. This g1 arrest is associated with down-regulation of a subset of e2f targets including cyclin a, e2f1 and cdc6. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
USP37 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265047 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21596315 |
USP37 was induced by E2F transcription factors in G1|Ectopic E2F1 activated the wild-type promoter, but not the mutant promoter, 3-fold over basal levels in 293T cells (Figure 4F), confirming the functionality of the E2F binding sites in the USP37 promoter. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
MT1G |
0.335 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254132 |
|
|
Homo sapiens |
Aorta Endothelium |
| pmid |
sentence |
| 15735762 |
The E2F transcription factors induce the expression of many genes in response to specific extracellular stimuli. Here, we show that human metallothionein 1G (hMT1G) promoter is upregulated by E2F1 upon VEGF stimulation of human aortic endothelial cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
NOX4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254134 |
|
|
Mus musculus |
Vascular Smooth Muscle Cell Line |
| pmid |
sentence |
| 18554521 |
Positive regulation of the NADPH oxidase NOX4 promoter in vascular smooth muscle cells by E2F |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ERK1/2 | up-regulates activity
phosphorylation
|
E2F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-233526 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23616010 |
Erk also undergoes rapid translocation into the nucleus, where it phosphorylates and activates a variety of transcription factor targets, including sp1, e2f, elk-1, and ap1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Luminal Breast Cancer, Malignant Melanoma, Pancreatic ductal adenocarcinoma (PDA) |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
DHFR |
0.533 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253853 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
PCNA |
0.492 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253856 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 14618416 |
To assess transactivating activity of E2F1/DP-1, we also analyzed expression of ten putative transcriptional targets of this complex in HCCs. Expression levels of TFDP1 and E2F1 correlated with those of seven transcriptional targets ( TYMS, DHFR, PCNA, RRM1, CCNE1, CDC2, and MYBL2) that play important roles in the G1/S transition, and down-regulation of TFDP1 inhibited growth of Hep3B cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DDB1 | up-regulates
binding
|
E2F1 |
0.353 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-54096 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 9418871 |
We show that ddb, a putative dna repair protein, associates with the activation domain of e2f1 / expression of ddb specifically stimulated e2f1-activated transcription |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
MCPH1 |
0.348 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253848 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22136275 |
Overexpression of E2F1 led to the upregulation of MCPH1 transcription, and knocking down the endogenous E2F1 resulted in the inhibition of the MCPH1 promoter activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | down-regulates quantity by repression
transcriptional regulation
|
TLR3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254136 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 22310660 |
Together, these data indicated that E2F1 suppresses TLR3 transcription, but during immune stimulation, Rb is upregulated to block the inhibitory effect of E2F1 on TLR3, highlighting a role of Rb-E2F1 axis in the innate immune response in epithelial cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
TFDP1 | up-regulates activity
binding
|
E2F1 |
0.812 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253865 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14618416 |
DP-1 is a heterodimerization partner for members of the E2F family of transcription factors; E2F/DP-1 regulates the expression of various cellular promoters, particularly gene products that are involved in the cell cycle. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
HIC1 |
0.29 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253844 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19491197 |
expression of E2F1 in the p53(-/-) hepatocellular carcinoma cell line Hep3B led to an increase of endogenous HIC1 mRNA, although bisulfite genomic sequencing of the HIC1 promoter revealed that the region bearing the two E2F1 binding sites is hypermethylated. In addition, endogenous E2F1 induced by etoposide treatment bound to the HIC1 promoter. Moreover, inhibition of E2F1 strongly reduced the expression of etoposide-induced HIC1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DDB2 | up-regulates
binding
|
E2F1 |
0.368 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-54102 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 9418871 |
We show that ddb, a putative dna repair protein, associates with the activation domain of e2f1 / expression of ddb specifically stimulated e2f1-activated transcription |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
LRBA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253846 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15064745 |
We also show that LRBA promoter activity and endogenous LRBA mRNA levels are reduced by p53 and increased by E2F1, indicating that mutations in the tumor suppressors p53 and Rb could contribute to the deregulation of LRBA. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HES6 | up-regulates quantity by expression
transcriptional regulation
|
E2F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-189101 |
|
|
Homo sapiens |
Breast Cancer Cell, Neuron |
| pmid |
sentence |
| 19891787 |
Expression of hes-6 resulted in induction of e2f-1, a crucial target gene for the transcriptional repressor hes-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RB1 | down-regulates activity
binding
|
E2F1 |
0.92 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-37305 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 8255752 |
E2f binds rb. E2f activation domain is the target for rb-induced repression. Rb can silence the 57 residue e2f activation domain. Rb can mask e2f residues involved in the activation process, possibly by mimicking a component of the transcriptional machinery |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Luminal Breast Cancer, Malignant Melanoma, Pancreatic ductal adenocarcinoma (PDA) |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
BBC3 |
0.428 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253843 |
|
|
Homo sapiens |
SK-MEL-2 Cell |
| pmid |
sentence |
| 17263886 |
Up-regulation of the PUMA gene and protein by E2F-1 overexpression was detected by real-time PCR and Western blot analysis in the SK-MEL-2 melanoma cell line |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression
transcriptional regulation
|
MUC4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254133 |
|
|
Homo sapiens |
Pancreatic Cancer Cell |
| pmid |
sentence |
| 22537161 |
Nicotine, IFN-γ and retinoic acid mediated induction of MUC4 in pancreatic cancer requires E2F1 and STAT-1 transcription factors and utilize different signaling cascades |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
E2F1
- 
- 