| + |
RAD18 | up-regulates activity 
ubiquitination
|
TP53BP1 |
0.615 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278593 |
Lys1268 |
DGTEVERkVTEETEE |
Homo sapiens |
|
| pmid |
sentence |
| 19228710 |
RAD18 associates with 53BP1 and is recruited to DSB sites in a 53BP1 dependent manner specifically during G1-phase, RAD18 monoubiquitinates KBD domain of 53BP1 at lysine 1268 in vitro.|These results suggest that RAD18 directed modification at Lysine 1268 of 53BP1 promotes the retention of 53BP1 at DSBs. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ATM | up-regulates
phosphorylation
|
TP53BP1 |
0.874 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-197611 |
Ser1219 |
DDTESLHsQGEEEFD |
Homo sapiens |
|
| pmid |
sentence |
| 22621922 |
Here we report phosphorylation of 53bp1 at several novel residues, using mass spectrometry and phospho-specific antibodies, and show that ionising radiation-stimulated phosphorylation of these residues requires atm. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-197615 |
Ser831 |
EPVEQDSsQPSLPLV |
Homo sapiens |
|
| pmid |
sentence |
| 22621922 |
Here we report phosphorylation of 53bp1 at several novel residues, using mass spectrometry and phospho-specific antibodies, and show that ionising radiation-stimulated phosphorylation of these residues requires atm. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-197619 |
Thr302 |
PEPEVLStQEDLFDQ |
Homo sapiens |
|
| pmid |
sentence |
| 22621922 |
Here we report phosphorylation of 53bp1 at several novel residues, using mass spectrometry and phospho-specific antibodies, and show that ionising radiation-stimulated phosphorylation of these residues requires atm. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-197622 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22621922 |
The kinase vrk1 is activated by dna double strand breaks induced by ionizing radiation (ir) and specifically phosphorylates 53bp1 in serum-starved cells. |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| Pathways: | DNA repair in cancer |
| + |
AURKB | up-regulates activity
phosphorylation
|
TP53BP1 |
0.413 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264411 |
Ser1342 |
GPLRGKTsGTEPADF |
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 28415769 |
Here we report for the first time that tumor suppressor p53-binding protein 1 (53BP1) is phosphorylated at serine 1342 (S1342) by Aurora kinase B both in vitro and in human cells, which is required for optimal recruitment of 53BP1 at kinetochores. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PPP4C | up-regulates activity
dephosphorylation
|
TP53BP1 |
0.359 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264451 |
Ser1618 |
LTKAADIsLDNLVEG |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |Depletion of PP4C, or PP4R3beta, causes persistence of phospho-T1609 and phospho-S1618 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264450 |
Thr1609 |
LGPYEAVtPLTKAAD |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |Depletion of PP4C, or PP4R3beta, causes persistence of phospho-T1609 and phospho-S1618 |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
PLK1 | down-regulates activity 
phosphorylation
|
TP53BP1 |
0.6 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264413 |
Ser1618 |
LTKAADIsLDNLVEG |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |Addition of the inhibitors for PLK1 and the p38 MAPK leads to a complete loss of pT1609/pS1618 signal within 3 hr in mitotic cells |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
GSK3B | up-regulates activity
phosphorylation
|
TP53BP1 |
0.284 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278226 |
Ser166 |
GAEDTASsQLGFGVL |
Homo sapiens |
|
| pmid |
sentence |
| 29328365 |
Based on these observations, we hypothesized that the IR induced GSK3beta nuclear translocation may activate 53BP1 via phosphorylation at the S/T-Q motif.|Importantly, our in vivo and in vitro data clearly indicated that GSK3\u03b2 induced the phosphorylation of 53BP1 at the Ser166 site. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CyclinB/CDK1 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.549 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264444 |
Ser1678 |
ITSEEERsPAKRGRK |
in vitro |
|
| pmid |
sentence |
| 30685087 |
Nuclear import of 53BP1 is required for proper localization of 53BP1 and maintenance of genome integrity. 53BP1 has a classical bipartite nuclear localization signal (NLS) of sequence 1666-GKRKLITSEEERSPAKRGRKS-1686. Ser1678 within the 53BP1 NLS can be phosphorylated by CDK1/cyclin B, and a phosphomimetic substitution of Ser1678 with aspartate has been shown to negatively regulate nuclear import of 53BP1. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
CDK1 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.603 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264412 |
Ser1678 |
ITSEEERsPAKRGRK |
in vitro |
|
| pmid |
sentence |
| 30685087 |
Nuclear import of 53BP1 is required for proper localization of 53BP1 and maintenance of genome integrity. 53BP1 has a classical bipartite nuclear localization signal (NLS) of sequence 1666-GKRKLITSEEERSPAKRGRKS-1686. Ser1678 within the 53BP1 NLS can be phosphorylated by CDK1/cyclin B, and a phosphomimetic substitution of Ser1678 with aspartate has been shown to negatively regulate nuclear import of 53BP1. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
ATM |
phosphorylation
|
TP53BP1 |
0.874 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-100641 |
Ser25 |
PCLIIEDsQPESQVL |
Homo sapiens |
|
| pmid |
sentence |
| 12697768 |
To examine whether the respective sq sites become phosphorylated in vivo, we raised polyclonal antibodies against phosphorylated ser-6 (anti-s6p), phosphorylated ser-25 and ser-29 (anti-s25p/29p), and phosphorylated ser-784 (anti-s784p). All affinity-purified antisera recognized 53bp1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-100645 |
Ser29 |
IEDSQPEsQVLEDDS |
Homo sapiens |
|
| pmid |
sentence |
| 12697768 |
To examine whether the respective sq sites become phosphorylated in vivo, we raised polyclonal antibodies against phosphorylated ser-6 (anti-s6p), phosphorylated ser-25 and ser-29 (anti-s25p/29p), and phosphorylated ser-784 (anti-s784p). All affinity-purified antisera recognized 53bp1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-100649 |
Ser6 |
sQLDSDFS |
Homo sapiens |
|
| pmid |
sentence |
| 12697768 |
To examine whether the respective sq sites become phosphorylated in vivo, we raised polyclonal antibodies against phosphorylated ser-6 (anti-s6p), phosphorylated ser-25 and ser-29 (anti-s25p/29p), and phosphorylated ser-784 (anti-s784p). All affinity-purified antisera recognized 53bp1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-100653 |
Ser784 |
GVEKCSDsQSWEDIA |
Homo sapiens |
|
| pmid |
sentence |
| 12697768 |
To examine whether the respective sq sites become phosphorylated in vivo, we raised polyclonal antibodies against phosphorylated ser-6 (anti-s6p), phosphorylated ser-25 and ser-29 (anti-s25p/29p), and phosphorylated ser-784 (anti-s784p). All affinity-purified antisera recognized 53bp1 |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| Pathways: | DNA repair in cancer |
| + |
MAPK12 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264448 |
Thr1609 |
LGPYEAVtPLTKAAD |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |phosphorylation of T1609 is likely to be mediated by p38 MAPK |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPK13 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264449 |
Thr1609 |
LGPYEAVtPLTKAAD |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |phosphorylation of T1609 is likely to be mediated by p38 MAPK |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPK14 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.281 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264446 |
Thr1609 |
LGPYEAVtPLTKAAD |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |phosphorylation of T1609 is likely to be mediated by p38 MAPK |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
p38 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264445 |
Thr1609 |
LGPYEAVtPLTKAAD |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |phosphorylation of T1609 is likely to be mediated by p38 MAPK |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPK11 | down-regulates activity
phosphorylation
|
TP53BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264447 |
Thr1609 |
LGPYEAVtPLTKAAD |
in vitro |
|
| pmid |
sentence |
| 24703952 |
Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |phosphorylation of T1609 is likely to be mediated by p38 MAPK |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
PPM1D | down-regulates activity
dephosphorylation
|
TP53BP1 |
0.395 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277046 |
Thr543 |
IDEDGENtQIEDTEP |
Homo sapiens |
|
| pmid |
sentence |
| 31619012 |
In addition, WIP1 dephosphorylates 53BP1 at Threonine 543 that was previously implicated in mediating interaction with RIF1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BRD2 | up-regulates activity
relocalization
|
TP53BP1 |
0.269 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262035 |
|
|
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 29018219 |
BRD2 is required to recruit 53BP1 to DSBs.|When BRD2 recruitment was blocked with shRNA or JQ1 (Fig. 3a and Supplementary Figure 3c) or a panel of BRD2 siRNAs (Supplementary Figure 3a), the recruitment of 53BP1 to DSBs was significantly delayed. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BRCA1-C complex | up-regulates activity
relocalization
|
TP53BP1 |
0.625 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263230 |
|
|
|
|
| pmid |
sentence |
| 25400280 |
Additional to the role of the BRCA1–C complex in 53BP1 repositioning and initiation of resection, end resection is extended to facilitate RPA loading and subsequent RPA‐Rad51 exchange prior to sister strand invasion |
|
| Publications: |
1 |
| + |
H1-2 | down-regulates activity
binding
|
TP53BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273833 |
|
|
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 22249259 |
Similarly, DNA-PK-mediated phosphorylation of H1.2 at T146 enhances p53 transcriptional activity by impeding H1.2 binding to p53 and thereby attenuating its suppressive effects on p53 transactivation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VRK1 | up-regulates
phosphorylation
|
TP53BP1 |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-197625 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22621922 |
The kinase vrk1 is activated by dna double strand breaks induced by ionizing radiation (ir) and specifically phosphorylates 53bp1 in serum-starved cells./ Vrk1 knockdown resulted in the defective formation of 53bp1 foci in response to ir both in number and size |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZNF420 | down-regulates activity
binding
|
TP53BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273512 |
|
|
Homo sapiens |
HCT-116 Cell |
| pmid |
sentence |
| 19377469 |
These results indicate that Apak is a genuine substrate of ATM kinase. Apak phosphorylation on Ser 68 is critical for p53-mediated apoptosis. in response to DNA damage, ATM is rapidly activated by autophosphorylation and mediates p53 activation through disruption of the Apak–p53 complex by phosphorylating Apak on Ser 68. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PIAS1 | up-regulates
sumoylation
|
TP53BP1 |
0.496 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-162156 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20016603 |
Pias1 and pias4 are recruited to dna-damage sites and mediate 53bp1 recruitment and sumoylation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PIAS4 | up-regulates
sumoylation
|
TP53BP1 |
0.634 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-162167 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20016603 |
Pias1 and pias4 are recruited to dna-damage sites and mediate 53bp1 recruitment and sumoylation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
L3MBTL1 | down-regulates activity
binding
|
TP53BP1 |
0.407 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262059 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 29018219 |
L3MBTL1, a tumor suppressor with high affinity for H4K20me2, can block 53BP1 binding at DSBs |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RNF168 | up-regulates quantity
ubiquitination
|
TP53BP1 |
0.822 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278777 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30953555 |
E3 ligase RNF168-mediated 53BP1 ubiquitination through activated the mechanistic target of rapamycin (mTOR)-ribosomal S6 kinase (S6K) signaling and increased 53BP1 protein stability in response to IR|We further found that overexpression of RNF168 enhanced 53BP1 ubiquitination inhibited by G0S2 overexpression in U87 and LN229 cells in response to IR (Fig. xref f). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TP53BP1 | up-regulates activity
binding
|
RIF1 |
0.683 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259058 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 23333305 |
RIF1 is recruited to DSBs via the N-terminal phospho-SQ/TQ domain of 53BP1, and DSBs generated by ionizing radiation or during CSR are hyperresected in the absence of RIF1. Thus, RIF1 and 53BP1 cooperate to block DSB resection to promote NHEJ in G1, which is antagonized by BRCA1 in S phase to ensure a switch of DSB repair mode to homologous recombination. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | DNA repair in cancer |
| + |
TP53BP1 |
binding
|
H4C1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-151654 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17190600 |
Here we demonstrate that this link occurs through direct binding of 53bp1 and crb2 to histone h4. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
TP53BP1
- 
- 