Relation Results

Summary

Name SMURF1
Full Name E3 ubiquitin-protein ligase SMURF1
Synonyms hSMURF1, HECT-type E3 ubiquitin transferase SMURF1, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1 | KIAA1625
Primary ID Q9HCE7
Links - -
Type protein
Relations 71
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 ...
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Type: Score: Layout: SPV 
0.5010.20.20.8820.20.20.2570.6670.2940.20.2810.6920.830.20.7060.330.20.20.830.20.6360.20.3930.20.2620.20.20.6120.20.8820.20.5580.2490.6580.5060.20.2630.3020.20.20.20.7440.20.20.20.20.2630.20.260.5570.20.20.20.20.7680.20.4120.20.7680.2Cullin 1-RBX1-Skp1SMURF1UVRAGMINDY3SMAD7SLAIN2ZFAND5TBX6SMAD9RAD23AIMPACTFAF2TGFBR1SMAD6FKBP3SMAD1NFICANKRD13DCTNND1ANKS4BPARD6/SMURF1RABEP2FBXO3ELOF1TOM1ODF2UBXN6TGFBR2ENTR1C7orf50BMPR2FOXO1BMPR1ARUNX2CALCOCO1DAB2IPUBQLN1PADI4C9orf78Ub:E2SMAD4ASCC2KRT36CUEDC1ILRUNSRSF5FERMT2TOM1L2FBXL15TPM4CCDC69OTUD6BHOMER2SMAD1/5/8ANKRD13ATRIB2ANXA6SMAD5PRR16

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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ Cullin 1-RBX1-Skp1down-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination SMURF1 (isoform 2) 0.501
Identifier Residue Sequence Organism Cell Line
SIGNOR-271911 LYS355 YERDLVQkLKVLRHE Homo sapiens HEK-293 Cell
pmid sentence
Here, we report that F-box and LRR domain-containing protein 15 (FBXL15), an F-box protein of the FBXL family, forms an Skp1-Cullin1-F-box protein-Roc1 (SCF)(FBXL15) ubiquitin ligase complex and targets Smurf1 for ubiquitination and proteasomal degradation.  FBXL15, through its leucine-rich repeat domain, specifically recognizes the large subdomain within the N-lobe of the Smurf1 HECT domain and promotes the ubiquitination of Smurf1 on K355 and K357 within the WW-HECT linker region. In this way, FBXL15 positively regulates BMP signalling in mammalian cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-271912 LYS357 RDLVQKLkVLRHELS Homo sapiens HEK-293 Cell
pmid sentence
Here, we report that F-box and LRR domain-containing protein 15 (FBXL15), an F-box protein of the FBXL family, forms an Skp1-Cullin1-F-box protein-Roc1 (SCF)(FBXL15) ubiquitin ligase complex and targets Smurf1 for ubiquitination and proteasomal degradation.  FBXL15, through its leucine-rich repeat domain, specifically recognizes the large subdomain within the N-lobe of the Smurf1 HECT domain and promotes the ubiquitination of Smurf1 on K355 and K357 within the WW-HECT linker region. In this way, FBXL15 positively regulates BMP signalling in mammalian cells.
Publications: 2 Organism: Homo Sapiens
+ SMURF1down-regulates activity img/direct_inhibition.png ubiquitination UVRAG 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273652 Lys517 ENERLQYkTPPPSYN Homo sapiens HEK-293 Cell
pmid sentence
Here we report that UVRAG is ubiquitinated by SMURF1 at lysine residues 517 and 559, which decreases the association of UVRAG with RUBCN and promotes autophagosome maturation. However, the deubiquitinase ZRANB1 specifically cleaves SMURF1-induced K29 and K33-linked polyubiquitin chains from UVRAG, thereby increasing the binding of UVRAG to RUBCN and inhibiting autophagy flux. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-273653 Lys559 DTSLDFSkENKKKGE Homo sapiens HEK-293 Cell
pmid sentence
Here we report that UVRAG is ubiquitinated by SMURF1 at lysine residues 517 and 559, which decreases the association of UVRAG with RUBCN and promotes autophagosome maturation. However, the deubiquitinase ZRANB1 specifically cleaves SMURF1-induced K29 and K33-linked polyubiquitin chains from UVRAG, thereby increasing the binding of UVRAG to RUBCN and inhibiting autophagy flux. 
Publications: 2 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination MINDY3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272675 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMAD7up-regulates activity img/direct-activation.png relocalization, binding SMURF1 0.882
Identifier Residue Sequence Organism Cell Line
SIGNOR-175269 Homo sapiens
pmid sentence
One of the major mechanisms underlying the inhibitory effect of Smad7 on TGF-_ signaling operates through accelerating T_RI turnover by recruiting ubiquitin E3 ligases such as Smurf1 and Smurf2
Identifier Residue Sequence Organism Cell Line
SIGNOR-185131 Homo sapiens
pmid sentence
Smad7 also recruits the HECT type of E3 ubiquitin ligases, Smurf1 and Smurf2. It binds to Smurfs in the nucleus and translocates into the cytoplasm in response to TGF-_ and recruits the ubiquitin ligases to the activated type I receptor ALK5/T_RI, leading to the degradation of the receptor through the proteasomal pathway.
Identifier Residue Sequence Organism Cell Line
SIGNOR-272942 Chlorocebus aethiops COS-7 Cell
pmid sentence
Here we show that Smurf1, an E3 ubiquitin ligase for bone morphogenetic protein-specific Smads, also interacts with Smad7 and induces Smad7 ubiquitination and translocation into the cytoplasm. In addition, Smurf1 associates with TbetaR-I via Smad7, with subsequent enhancement of turnover of TbetaR-I and Smad7. 
Publications: 3 Organism: Homo Sapiens, Chlorocebus Aethiops
+ SMURF1 img/unknown.png ubiquitination SLAIN2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272696 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination ZFAND5 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272676 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination TBX6 0.257
Identifier Residue Sequence Organism Cell Line
SIGNOR-272784 Homo sapiens HEK-293 Cell
pmid sentence
Smad6 mediates Tbx6 ubiquitination and proteasomal degradation. Tbx6 forms a ternary complex with Smad6 and Smurf1. Here, we report that Tbx6 interacts directly with Smad6, an inhibitory Smad that antagonizes the BMP signal. This interaction is mediated through the Mad homology 2 (MH2) domain of Smad6 and residues 90-180 of Tbx6. We demonstrate that Smad6 facilitates the degradation of Tbx6 protein through recruitment of Smurf1, a ubiquitin E3 ligase.
Publications: 1 Organism: Homo Sapiens
+ SMURF1down-regulates img/direct_inhibition.png ubiquitination SMAD9 0.667
Identifier Residue Sequence Organism Cell Line
SIGNOR-195669 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degra-dation of smads and receptors for tgf-beta and bmps
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination RAD23A 0.294
Identifier Residue Sequence Organism Cell Line
SIGNOR-272691 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination IMPACT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272686 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination FAF2 0.281
Identifier Residue Sequence Organism Cell Line
SIGNOR-272698 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates activity img/direct_inhibition.png ubiquitination TGFBR1 0.692
Identifier Residue Sequence Organism Cell Line
SIGNOR-153414 Homo sapiens
pmid sentence
Recruitment of WW and HECT domain E3-ubiquitin ligases Smurf1 and 2 to induce type I receptor ubiquitination and subsequent receptor degradation;
Publications: 1 Organism: Homo Sapiens
+ SMAD6up-regulates activity img/direct-activation.png binding SMURF1 0.83
Identifier Residue Sequence Organism Cell Line
SIGNOR-272786 Homo sapiens HEK-293 Cell
pmid sentence
Smad6 mediates Tbx6 ubiquitination and proteasomal degradation. Tbx6 forms a ternary complex with Smad6 and Smurf1. Here, we report that Tbx6 interacts directly with Smad6, an inhibitory Smad that antagonizes the BMP signal. This interaction is mediated through the Mad homology 2 (MH2) domain of Smad6 and residues 90-180 of Tbx6. We demonstrate that Smad6 facilitates the degradation of Tbx6 protein through recruitment of Smurf1, a ubiquitin E3 ligase.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination FKBP3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272694 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates img/direct_inhibition.png ubiquitination SMAD1 0.706
Identifier Residue Sequence Organism Cell Line
SIGNOR-195660 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps
Publications: 1 Organism: Homo Sapiens
+ SMURF1down-regulates quantity img/direct_inhibition.png ubiquitination NFIC 0.33
Identifier Residue Sequence Organism Cell Line
SIGNOR-278753 Homo sapiens
pmid sentence
In addition, Smurf1 knockdown also blocked the degradation of endogenous NFI-C in response to TGF-beta1 (XREF_FIG).|In particular, Smurf1 and Smurf2 markedly increased the polyubiquitination of NFI-C in the presence of TGF-beta1 (XREF_FIG and XREF_SUPPLEMENTARY).
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ANKRD13D 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272677 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates activity img/direct_inhibition.png monoubiquitination CTNND1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277507 Canis lupus familiaris MDCK Cell
pmid sentence
 Upon TGFβ treatment, activated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylates T900 of p120-catenin to promote its interaction with Smurf1 and subsequent monoubiquitination. TGFβ promotes monoubiquitination of p120-catenin through Smurf1 to induce junction dissociation.
Publications: 1 Organism: Canis Lupus Familiaris
+ SMURF1down-regulates activity img/direct_inhibition.png relocalization SMAD6 0.83
Identifier Residue Sequence Organism Cell Line
SIGNOR-105931 Homo sapiens
pmid sentence
Smurf1, with its WW domain, specifically binds to the PY motif of Smad6 and transports Smad6 into the cytoplasm.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ANKS4B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272678 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1form complex img/form-complex.png binding PARD6/SMURF1 0.636
Identifier Residue Sequence Organism Cell Line
SIGNOR-227562 Homo sapiens NMuMg Cell
pmid sentence
The Par6-Smurf1 complex then mediates the localized ubiquitination of RhoA to enable the TGF_-dependent dissolution of tight junctions during EMT.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination RABEP2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272690 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ FBXO3down-regulates quantity by destabilization img/direct_inhibition.png binding SMURF1 0.393
Identifier Residue Sequence Organism Cell Line
SIGNOR-272441 Homo sapiens HEK-293 Cell
pmid sentence
F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation. Here we show that another F-box protein Fbxo3, belonging to the FBXO type protein family, also interacts with and targets Smurf1 for poly-ubiquitination and proteasomal degradation. The SCF complex is composed of F-box protein, Skp1, Cullin1 (Cul1) and ROC1. Fbxo3, whose substrates are few, forms SCF Fbxo3 ubiquitin ligase and regulates the degradations of Fbxl2, p62, HIPK2 and p300 through the ubiquitin-proteasome pathway.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ELOF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272685 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination TOM1 0.262
Identifier Residue Sequence Organism Cell Line
SIGNOR-272679 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination ODF2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272683 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination UBXN6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272702 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates activity img/direct_inhibition.png ubiquitination TGFBR2 0.612
Identifier Residue Sequence Organism Cell Line
SIGNOR-195672 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ENTR1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272705 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination SMAD7 0.882
Identifier Residue Sequence Organism Cell Line
SIGNOR-272941 Chlorocebus aethiops COS-7 Cell
pmid sentence
Here we show that Smurf1, an E3 ubiquitin ligase for bone morphogenetic protein-specific Smads, also interacts with Smad7 and induces Smad7 ubiquitination and translocation into the cytoplasm. In addition, Smurf1 associates with TbetaR-I via Smad7, with subsequent enhancement of turnover of TbetaR-I and Smad7. 
Publications: 1 Organism: Chlorocebus Aethiops
+ SMURF1 img/unknown.png ubiquitination C7orf50 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272693 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates img/direct_inhibition.png ubiquitination BMPR2 0.558
Identifier Residue Sequence Organism Cell Line
SIGNOR-195654 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps.
Identifier Residue Sequence Organism Cell Line
SIGNOR-153402 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps.
Publications: 2 Organism: Homo Sapiens
+ FOXO1up-regulates quantity img/direct-activation.png transcriptional regulation SMURF1 0.249
Identifier Residue Sequence Organism Cell Line
SIGNOR-256258 Mus musculus C2C12 Cell
pmid sentence
The IGF-1/PI3K/Akt pathway, which has been shown to induce hypertrophy, prevents induction of requisite atrophy mediators, namely the muscle-specific ubiquitin ligases MAFbx and MuRF1. Moreover, the mechanism for this inhibition involves Akt-mediated inhibition of the FoxO family of transcription factors;
Identifier Residue Sequence Organism Cell Line
SIGNOR-256268 Mus musculus Skeletal Muscle
pmid sentence
FoxO factors are required for the transcriptional regulation of the ubiquitin ligases atrogin-1, also called muscle atrophy F-box (MAFbx) and muscle ring finger 1 (MuRF1), leading to the ubiquitylation of myosin and other muscle proteins (see below), and their degradation via the proteasome
Publications: 2 Organism: Mus Musculus
+ SMURF1down-regulates img/direct_inhibition.png ubiquitination BMPR1A 0.658
Identifier Residue Sequence Organism Cell Line
SIGNOR-153399 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps.
Identifier Residue Sequence Organism Cell Line
SIGNOR-195651 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps.
Publications: 2 Organism: Homo Sapiens
+ SMURF1down-regulates activity img/direct_inhibition.png ubiquitination RUNX2 0.506
Identifier Residue Sequence Organism Cell Line
SIGNOR-236083 Homo sapiens
pmid sentence
Smurf1 interacts directly with Cbfa1 and mediates Cbfa1 degradation in a ubiquitin- and proteasome-dependent manner.
Identifier Residue Sequence Organism Cell Line
SIGNOR-95233 Homo sapiens
pmid sentence
Recently we have found that smurf1 mediates the protein degradation of the osteoblast-specific transcription factor runx2/cbfa1.
Publications: 2 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination CALCOCO1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272688 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination DAB2IP 0.263
Identifier Residue Sequence Organism Cell Line
SIGNOR-254776 Homo sapiens
pmid sentence
DAB2IP protein levels can be negatively regulated by the activity of the E3-ubiquitin ligases Fbw7, Skp2, and Smurf1
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination UBQLN1 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-272701 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination PADI4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272689 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination C9orf78 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272674 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ Ub:E2up-regulates activity img/direct-activation.png ubiquitination SMURF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-271290 Homo sapiens
pmid sentence
The ubiquitination process is mediated sequentially by three classes of enzymes consisting of a Ub-activating enzyme E1, a Ub-conjugating enzyme E2, and a Ub ligase E3. Ub is first activated by E1 in an adenosine 5′-triphosphate (ATP)-dependent manner t
Publications: 1 Organism: Homo Sapiens
+ SMURF1down-regulates activity img/direct_inhibition.png ubiquitination SMAD4 0.744
Identifier Residue Sequence Organism Cell Line
SIGNOR-236096 Homo sapiens HEK-293T Cell
pmid sentence
Smurfs, which otherwise cannot directly bind to smad4, mediated poly-ubiquitination of smad4 in the presence of smad6 or smad7. Smad signaling is negatively regulated by inhibitory (i) smads and ubiquitin-mediated processes.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ASCC2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272692 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates activity img/direct_inhibition.png ubiquitination SMAD7 0.882
Identifier Residue Sequence Organism Cell Line
SIGNOR-97064 Homo sapiens
pmid sentence
Smad ubiquitin regulatory factor 1 (Smurf1), a HECT type E3 ubiquitin ligase, interacts with inhibitory Smad7 and induces translocation of Smad7 to the cytoplasm
Publications: 1 Organism: Homo Sapiens
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination TGFBR1 0.692
Identifier Residue Sequence Organism Cell Line
SIGNOR-272943 Chlorocebus aethiops COS-7 Cell
pmid sentence
Here we show that Smurf1, an E3 ubiquitin ligase for bone morphogenetic protein-specific Smads, also interacts with Smad7 and induces Smad7 ubiquitination and translocation into the cytoplasm. In addition, Smurf1 associates with TbetaR-I via Smad7, with subsequent enhancement of turnover of TbetaR-I and Smad7. 
Publications: 1 Organism: Chlorocebus Aethiops
+ SMURF1 img/unknown.png ubiquitination KRT36 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272695 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination CUEDC1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272682 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination ILRUN 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272697 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination SRSF5 0.263
Identifier Residue Sequence Organism Cell Line
SIGNOR-278665 Homo sapiens
pmid sentence
K125 was also the major site of SRSF5 ubiquitylation mediated by Smurf1.|Smurf1 targets SRSF5 for degradation upon low glucose
Publications: 1 Organism: Homo Sapiens
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination FERMT2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-278614 Homo sapiens
pmid sentence
Smurf1 mediates Kindlin-2 proteasomal degradation.|Smurf1 promotes polyubiquitination of Kindlin-2.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination TOM1L2 0.26
Identifier Residue Sequence Organism Cell Line
SIGNOR-272700 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ FBXL15down-regulates quantity by destabilization img/direct_inhibition.png binding SMURF1 (isoform 2) 0.557
Identifier Residue Sequence Organism Cell Line
SIGNOR-271909 Homo sapiens HEK-293 Cell
pmid sentence
Here, we report that F-box and LRR domain-containing protein 15 (FBXL15), an F-box protein of the FBXL family, forms an Skp1-Cullin1-F-box protein-Roc1 (SCF)(FBXL15) ubiquitin ligase complex and targets Smurf1 for ubiquitination and proteasomal degradation.  FBXL15, through its leucine-rich repeat domain, specifically recognizes the large subdomain within the N-lobe of the Smurf1 HECT domain and promotes the ubiquitination of Smurf1 on K355 and K357 within the WW-HECT linker region. In this way, FBXL15 positively regulates BMP signalling in mammalian cells.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination TPM4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272684 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination CCDC69 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272704 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1 img/unknown.png ubiquitination OTUD6B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272687 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ Cullin 1-RBX1-Skp1down-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination SMURF1 0.501
Identifier Residue Sequence Organism Cell Line
SIGNOR-272443 Homo sapiens HEK-293 Cell
pmid sentence
F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation. Here we show that another F-box protein Fbxo3, belonging to the FBXO type protein family, also interacts with and targets Smurf1 for poly-ubiquitination and proteasomal degradation. The SCF complex is composed of F-box protein, Skp1, Cullin1 (Cul1) and ROC1. Fbxo3, whose substrates are few, forms SCF Fbxo3 ubiquitin ligase and regulates the degradations of Fbxl2, p62, HIPK2 and p300 through the ubiquitin-proteasome pathway.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination HOMER2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272699 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates img/direct_inhibition.png ubiquitination SMAD1/5/8 0.768
Identifier Residue Sequence Organism Cell Line
SIGNOR-269847 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ANKRD13A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272680 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination TRIB2 0.412
Identifier Residue Sequence Organism Cell Line
SIGNOR-275432 Homo sapiens HEK-293T Cell
pmid sentence
 In this study, we found that TRIB2 was up-regulated and exhibited high stability in liver cancer cells compared with other cells. We performed a structure-function analysis of TRIB2 and identified a domain (amino acids 1-5) at the N terminus that interacted with the E3 ubiquitin ligase Smurf1 and was critical for protein stability. Deletion of this domain extended TRIB2 half-life time accompanied with a more significant malignant property compared with wild type TRIB2.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination ANXA6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272681 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
+ SMURF1down-regulates img/direct_inhibition.png ubiquitination SMAD5 0.768
Identifier Residue Sequence Organism Cell Line
SIGNOR-195663 Homo sapiens
pmid sentence
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps.
Publications: 1 Organism: Homo Sapiens
+ SMURF1 img/unknown.png ubiquitination PRR16 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272703 in vitro
pmid sentence
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay.
Publications: 1 Organism: In Vitro
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