| + |
Cullin 1-RBX1-Skp1 | down-regulates quantity by destabilization 
polyubiquitination
|
SMURF1 (isoform 2) |
0.501 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271911 |
LYS355 |
YERDLVQkLKVLRHE |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21572392 |
Here, we report that F-box and LRR domain-containing protein 15 (FBXL15), an F-box protein of the FBXL family, forms an Skp1-Cullin1-F-box protein-Roc1 (SCF)(FBXL15) ubiquitin ligase complex and targets Smurf1 for ubiquitination and proteasomal degradation. FBXL15, through its leucine-rich repeat domain, specifically recognizes the large subdomain within the N-lobe of the Smurf1 HECT domain and promotes the ubiquitination of Smurf1 on K355 and K357 within the WW-HECT linker region. In this way, FBXL15 positively regulates BMP signalling in mammalian cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271912 |
LYS357 |
RDLVQKLkVLRHELS |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21572392 |
Here, we report that F-box and LRR domain-containing protein 15 (FBXL15), an F-box protein of the FBXL family, forms an Skp1-Cullin1-F-box protein-Roc1 (SCF)(FBXL15) ubiquitin ligase complex and targets Smurf1 for ubiquitination and proteasomal degradation. FBXL15, through its leucine-rich repeat domain, specifically recognizes the large subdomain within the N-lobe of the Smurf1 HECT domain and promotes the ubiquitination of Smurf1 on K355 and K357 within the WW-HECT linker region. In this way, FBXL15 positively regulates BMP signalling in mammalian cells. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates activity
ubiquitination
|
UVRAG |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273652 |
Lys517 |
ENERLQYkTPPPSYN |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 30686098 |
Here we report that UVRAG is ubiquitinated by SMURF1 at lysine residues 517 and 559, which decreases the association of UVRAG with RUBCN and promotes autophagosome maturation. However, the deubiquitinase ZRANB1 specifically cleaves SMURF1-induced K29 and K33-linked polyubiquitin chains from UVRAG, thereby increasing the binding of UVRAG to RUBCN and inhibiting autophagy flux. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273653 |
Lys559 |
DTSLDFSkENKKKGE |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 30686098 |
Here we report that UVRAG is ubiquitinated by SMURF1 at lysine residues 517 and 559, which decreases the association of UVRAG with RUBCN and promotes autophagosome maturation. However, the deubiquitinase ZRANB1 specifically cleaves SMURF1-induced K29 and K33-linked polyubiquitin chains from UVRAG, thereby increasing the binding of UVRAG to RUBCN and inhibiting autophagy flux. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ZFAND5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272676 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates quantity by destabilization
polyubiquitination
|
TBX6 |
0.257 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272784 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19561075 |
Smad6 mediates Tbx6 ubiquitination and proteasomal degradation. Tbx6 forms a ternary complex with Smad6 and Smurf1. Here, we report that Tbx6 interacts directly with Smad6, an inhibitory Smad that antagonizes the BMP signal. This interaction is mediated through the Mad homology 2 (MH2) domain of Smad6 and residues 90-180 of Tbx6. We demonstrate that Smad6 facilitates the degradation of Tbx6 protein through recruitment of Smurf1, a ubiquitin E3 ligase. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
UBQLN1 |
0.302 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272701 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates
ubiquitination
|
SMAD9 |
0.668 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195669 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degra-dation of smads and receptors for tgf-beta and bmps |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
RAD23A |
0.294 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272691 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
IMPACT |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272686 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
FAF2 |
0.281 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272698 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
PADI4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272689 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates activity
ubiquitination
|
TGFBR1 |
0.692 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-153414 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17317136 |
Recruitment of WW and HECT domain E3-ubiquitin ligases Smurf1 and 2 to induce type I receptor ubiquitination and subsequent receptor degradation; |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMAD6 | up-regulates activity 
binding
|
SMURF1 |
0.83 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272786 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19561075 |
Smad6 mediates Tbx6 ubiquitination and proteasomal degradation. Tbx6 forms a ternary complex with Smad6 and Smurf1. Here, we report that Tbx6 interacts directly with Smad6, an inhibitory Smad that antagonizes the BMP signal. This interaction is mediated through the Mad homology 2 (MH2) domain of Smad6 and residues 90-180 of Tbx6. We demonstrate that Smad6 facilitates the degradation of Tbx6 protein through recruitment of Smurf1, a ubiquitin E3 ligase. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
FKBP3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272694 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates
ubiquitination
|
SMAD1 |
0.706 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195660 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates quantity
ubiquitination
|
NFIC |
0.33 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278753 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22195013 |
In addition, Smurf1 knockdown also blocked the degradation of endogenous NFI-C in response to TGF-beta1 (XREF_FIG).|In particular, Smurf1 and Smurf2 markedly increased the polyubiquitination of NFI-C in the presence of TGF-beta1 (XREF_FIG and XREF_SUPPLEMENTARY). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ANKRD13D |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272677 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates activity
monoubiquitination
|
CTNND1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277507 |
|
|
Canis lupus familiaris |
MDCK Cell |
| pmid |
sentence |
| 32010791 |
Upon TGFβ treatment, activated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylates T900 of p120-catenin to promote its interaction with Smurf1 and subsequent monoubiquitination. TGFβ promotes monoubiquitination of p120-catenin through Smurf1 to induce junction dissociation. |
|
| Publications: |
1 |
Organism: |
Canis Lupus Familiaris |
| + |
SMAD7 | up-regulates activity
relocalization, binding
|
SMURF1 |
0.881 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-185131 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19352540 |
Smad7 also recruits the HECT type of E3 ubiquitin ligases, Smurf1 and Smurf2. It binds to Smurfs in the nucleus and translocates into the cytoplasm in response to TGF-_ and recruits the ubiquitin ligases to the activated type I receptor ALK5/T_RI, leading to the degradation of the receptor through the proteasomal pathway. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272942 |
|
|
Chlorocebus aethiops |
COS-7 Cell |
| pmid |
sentence |
| 11278251 |
Here we show that Smurf1, an E3 ubiquitin ligase for bone morphogenetic protein-specific Smads, also interacts with Smad7 and induces Smad7 ubiquitination and translocation into the cytoplasm. In addition, Smurf1 associates with TbetaR-I via Smad7, with subsequent enhancement of turnover of TbetaR-I and Smad7. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-175269 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21791611 |
One of the major mechanisms underlying the inhibitory effect of Smad7 on TGF-_ signaling operates through accelerating T_RI turnover by recruiting ubiquitin E3 ligases such as Smurf1 and Smurf2 |
|
| Publications: |
3 |
Organism: |
Homo Sapiens, Chlorocebus Aethiops |
| + |
SMURF1 |
ubiquitination
|
C9orf78 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272674 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates activity
relocalization
|
SMAD6 |
0.83 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-105931 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1, with its WW domain, specifically binds to the PY motif of Smad6 and transports Smad6 into the cytoplasm. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ANKS4B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272678 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | form complex 
binding
|
PARD6/SMURF1 |
0.636 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-227562 |
|
|
Homo sapiens |
NMuMg Cell |
| pmid |
sentence |
| 15761148 |
The Par6-Smurf1 complex then mediates the localized ubiquitination of RhoA to enable the TGF_-dependent dissolution of tight junctions during EMT. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
RABEP2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272690 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
FBXO3 | down-regulates quantity by destabilization
binding
|
SMURF1 |
0.393 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272441 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25721664 |
F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation. Here we show that another F-box protein Fbxo3, belonging to the FBXO type protein family, also interacts with and targets Smurf1 for poly-ubiquitination and proteasomal degradation. The SCF complex is composed of F-box protein, Skp1, Cullin1 (Cul1) and ROC1. Fbxo3, whose substrates are few, forms SCF Fbxo3 ubiquitin ligase and regulates the degradations of Fbxl2, p62, HIPK2 and p300 through the ubiquitin-proteasome pathway. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ELOF1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272685 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
TOM1 |
0.262 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272679 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
ODF2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272683 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
UBXN6 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272702 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates activity
ubiquitination
|
TGFBR2 |
0.612 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195672 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ENTR1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272705 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates quantity by destabilization
polyubiquitination
|
SMAD7 |
0.881 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272941 |
|
|
Chlorocebus aethiops |
COS-7 Cell |
| pmid |
sentence |
| 11278251 |
Here we show that Smurf1, an E3 ubiquitin ligase for bone morphogenetic protein-specific Smads, also interacts with Smad7 and induces Smad7 ubiquitination and translocation into the cytoplasm. In addition, Smurf1 associates with TbetaR-I via Smad7, with subsequent enhancement of turnover of TbetaR-I and Smad7. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
SMURF1 |
ubiquitination
|
C7orf50 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272693 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates
ubiquitination
|
BMPR2 |
0.558 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195654 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-153402 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17317136 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
FOXO1 | up-regulates quantity
transcriptional regulation
|
SMURF1 |
0.249 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256258 |
|
|
Mus musculus |
C2C12 Cell |
| pmid |
sentence |
| 15125842 |
The IGF-1/PI3K/Akt pathway, which has been shown to induce hypertrophy, prevents induction of requisite atrophy mediators, namely the muscle-specific ubiquitin ligases MAFbx and MuRF1. Moreover, the mechanism for this inhibition involves Akt-mediated inhibition of the FoxO family of transcription factors; |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256268 |
|
|
Mus musculus |
Skeletal Muscle |
| pmid |
sentence |
| 21798082 |
FoxO factors are required for the transcriptional regulation of the ubiquitin ligases atrogin-1, also called muscle atrophy F-box (MAFbx) and muscle ring finger 1 (MuRF1), leading to the ubiquitylation of myosin and other muscle proteins (see below), and their degradation via the proteasome |
|
| Publications: |
2 |
Organism: |
Mus Musculus |
| + |
SMURF1 | down-regulates
ubiquitination
|
BMPR1A |
0.658 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-153399 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17317136 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195651 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates activity
ubiquitination
|
RUNX2 |
0.507 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236083 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12738770 |
Smurf1 interacts directly with Cbfa1 and mediates Cbfa1 degradation in a ubiquitin- and proteasome-dependent manner. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-95233 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14701828 |
Recently we have found that smurf1 mediates the protein degradation of the osteoblast-specific transcription factor runx2/cbfa1. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
CALCOCO1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272688 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates quantity by destabilization
ubiquitination
|
DAB2IP |
0.263 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254776 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27858941 |
DAB2IP protein levels can be negatively regulated by the activity of the E3-ubiquitin ligases Fbw7, Skp2, and Smurf1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Ub:E2 | up-regulates activity
ubiquitination
|
SMURF1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271290 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 34199813 |
The ubiquitination process is mediated sequentially by three classes of enzymes consisting of a Ub-activating enzyme E1, a Ub-conjugating enzyme E2, and a Ub ligase E3. Ub is first activated by E1 in an adenosine 5′-triphosphate (ATP)-dependent manner t |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates activity
ubiquitination
|
SMAD4 |
0.744 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236096 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 15817471 |
Smurfs, which otherwise cannot directly bind to smad4, mediated poly-ubiquitination of smad4 in the presence of smad6 or smad7. Smad signaling is negatively regulated by inhibitory (i) smads and ubiquitin-mediated processes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ASCC2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272692 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates activity
ubiquitination
|
SMAD7 |
0.881 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-97064 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12519765 |
Smad ubiquitin regulatory factor 1 (Smurf1), a HECT type E3 ubiquitin ligase, interacts with inhibitory Smad7 and induces translocation of Smad7 to the cytoplasm |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates quantity by destabilization
polyubiquitination
|
TGFBR1 |
0.692 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272943 |
|
|
Chlorocebus aethiops |
COS-7 Cell |
| pmid |
sentence |
| 11278251 |
Here we show that Smurf1, an E3 ubiquitin ligase for bone morphogenetic protein-specific Smads, also interacts with Smad7 and induces Smad7 ubiquitination and translocation into the cytoplasm. In addition, Smurf1 associates with TbetaR-I via Smad7, with subsequent enhancement of turnover of TbetaR-I and Smad7. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
SMURF1 |
ubiquitination
|
KRT36 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272695 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
CUEDC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272682 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
ILRUN |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272697 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates quantity by destabilization
ubiquitination
|
FERMT2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278614 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28408404 |
Smurf1 mediates Kindlin-2 proteasomal degradation.|Smurf1 promotes polyubiquitination of Kindlin-2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
TOM1L2 |
0.26 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272700 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
FBXL15 | down-regulates quantity by destabilization
binding
|
SMURF1 (isoform 2) |
0.557 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271909 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21572392 |
Here, we report that F-box and LRR domain-containing protein 15 (FBXL15), an F-box protein of the FBXL family, forms an Skp1-Cullin1-F-box protein-Roc1 (SCF)(FBXL15) ubiquitin ligase complex and targets Smurf1 for ubiquitination and proteasomal degradation. FBXL15, through its leucine-rich repeat domain, specifically recognizes the large subdomain within the N-lobe of the Smurf1 HECT domain and promotes the ubiquitination of Smurf1 on K355 and K357 within the WW-HECT linker region. In this way, FBXL15 positively regulates BMP signalling in mammalian cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
TPM4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272684 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
OTUD6B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272687 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
CCDC69 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272704 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
Cullin 1-RBX1-Skp1 | down-regulates quantity by destabilization
polyubiquitination
|
SMURF1 |
0.501 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272443 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25721664 |
F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation. Here we show that another F-box protein Fbxo3, belonging to the FBXO type protein family, also interacts with and targets Smurf1 for poly-ubiquitination and proteasomal degradation. The SCF complex is composed of F-box protein, Skp1, Cullin1 (Cul1) and ROC1. Fbxo3, whose substrates are few, forms SCF Fbxo3 ubiquitin ligase and regulates the degradations of Fbxl2, p62, HIPK2 and p300 through the ubiquitin-proteasome pathway. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
HOMER2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272699 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates quantity by destabilization
ubiquitination
|
SRSF5 |
0.263 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278665 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 29942010 |
K125 was also the major site of SRSF5 ubiquitylation mediated by Smurf1.|Smurf1 targets SRSF5 for degradation upon low glucose |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates quantity by destabilization
ubiquitination
|
TRIB2 |
0.412 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275432 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 24089522 |
In this study, we found that TRIB2 was up-regulated and exhibited high stability in liver cancer cells compared with other cells. We performed a structure-function analysis of TRIB2 and identified a domain (amino acids 1-5) at the N terminus that interacted with the E3 ubiquitin ligase Smurf1 and was critical for protein stability. Deletion of this domain extended TRIB2 half-life time accompanied with a more significant malignant property compared with wild type TRIB2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 | down-regulates
ubiquitination
|
SMAD1/5/8 |
0.768 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269847 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
ANKRD13A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272680 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
ANXA6 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272681 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
PRR16 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272703 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 | down-regulates
ubiquitination
|
SMAD5 |
0.768 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195663 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degradation of smads and receptors for tgf-beta and bmps. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMURF1 |
ubiquitination
|
MINDY3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272675 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SMURF1 |
ubiquitination
|
SLAIN2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272696 |
|
|
in vitro |
|
| pmid |
sentence |
| 20804422 |
Recombinant proteins were used to profile substrate ubiquitination by the Smurf1 ubiquitin ligase on a global scale using protein microarrays. T Proteins ubiquitinated on the protein microarray were confirmed as potential substrates of the Smurf1 activity using an off chip in vitro ubiquitination assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
3.0
SMURF1
- 
- 