| + |
STUB1 | down-regulates quantity by destabilization 
ubiquitination
|
CBX4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277513 |
Lys178 |
LQYQGGHkEAPSPTC |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 32111827 |
The phosphorylation of CBX4 at T437 by casein kinase 1α (CK1α) facilitated its ubiquitination at both K178 and K280 and subsequent degradation by CHIP, and this phosphorylation of CBX4 could be reduced by TNFα. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277514 |
Lys280 |
GMQAVKIkSGEVAEG |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 32111827 |
The phosphorylation of CBX4 at T437 by casein kinase 1α (CK1α) facilitated its ubiquitination at both K178 and K280 and subsequent degradation by CHIP, and this phosphorylation of CBX4 could be reduced by TNFα. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
POU5F1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278562 |
Lys284 |
WFCNRRQkGKRSSSD |
Homo sapiens |
|
| pmid |
sentence |
| 29511337 |
CHIP overexpression decreased OCT4 stability through proteasomal degradation.|CHIP E3 ligase ubiquitinates OCT4 at lysine 284.|These data suggest that CHIP induces OCT4 ubiquitination and degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
FMR1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278599 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25268320 |
The results showed that FMR1 was ubiquitinated by CHIP WT or CHIP K30A. Also, ubiquitinated FMR1 accumulated in the presence of MG132, indicating that CHIP ubiquitinates FMR1 for proteasomal degradation ( Fig. 3 B). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
RUNX2 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278600 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18541707 |
Here, we show that CHIP promotes Runx2 ubiquitination and degradation and thereby negatively regulates osteoblast differentiation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
LRRK2 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278615 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19536328 |
CHIP can ubiquitinate LRRK2.|These results indicate that both the chaperone interaction and the ubiquitin ligase activity of CHIP are required for CHIP mediated degradation of LRRK2 protein. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination, polyubiquitination
|
ERBB2 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278645 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17239458 |
The ErbB2 kinase domain is required for GA-induced and CHIP-dependent ErbB2 ubiquitination and degradation . |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272586 |
|
|
in vitro |
|
| pmid |
sentence |
| 12239347 |
We now show that the chaperone-binding ubiquitin ligase CHIP efficiently ubiquitinates and down-regulates ErbB2. CHIP expression shortens the half-life of both nascent and mature ErbB2 protein. In vitro ubiquitination assay shows that CHIP serves as a ubiquitin ligase for ErbB2, and both exogenously expressed and endogenous CHIP coprecipitate with the kinase. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens, In Vitro |
| + |
STUB1 | up-regulates activity 
ubiquitination
|
INO80 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278646 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 33658435 |
Then, by an in vivo ubiquitination assay under denaturing conditions (hereafter, all in vivo ubiquitination assays were carried out under denaturing conditions), we determined whether CHIP ubiquitinates Ino80.|We also show that CHIP works together with BAP1 to enhance the stabilization of Ino80, leading to its chromatin binding. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
ATXN1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278666 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16831871 |
CHIP protects from the neurotoxicity of expanded and wild-type ataxin-1 and promotes their ubiquitination and degradation|Interestingly, CHIP also interacts with and ubiquitinates unexpanded ataxin-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
ATXN3 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278667 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21625540 |
Although our data show that CHIP may associate with Atx3 to ubiquitinate Atx3 in vitro, we still wonder whether CHIP is directly involved in the degradation of Atx3.|As a result, silencing of CHIP significantly increases the amount of Atx3 (XREF_FIG), suggesting that CHIP may down-regulate the Atx3 level. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination, polyubiquitination
|
CFTR |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278668 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25879443 |
Our results indicate that the post-endocytic ubiquitination of CFTR by CHIP is a critical step in the peripheral quality control of cell surface DeltaF508 CFTR. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272584 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 11146634 |
Here we show that CHIP functions with Hsc70 to sense the folded state of CFTR and targets aberrant forms for proteasomal degradation by promoting their ubiquitination. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
EIF4E |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278669 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28852129 |
This collaborative activity of cIAP1 and CHIP directs eIF4E toward degradation, controlling its levels and suppressing tumorigenesis.|We next sought to investigate whether eIF4E ubiquitination is enhanced by the collaborative activity of cIAP1 and CHIP, which we define as both the E3 ligase activity of cIAP1 alone and the E3 ligase activity of cIAP1 and CHIP together. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
TRAF6 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278670 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 24578159 |
CHIP promotes TRAF6 ubiquitination and degradation.|These results suggest that CHIP negatively regulates the stability of TRAF6. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity
ubiquitination
|
BACE1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278719 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25773675 |
This result establishes that CHIP negatively regulates BACE1 stability.|Thus, both deletion mutants of CHIP could not enhance the ubiquitination of BACE1, suggesting that both domains of CHIP were essential for ubiquitination and degradation of BACE1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | up-regulates activity
ubiquitination
|
CARD11 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278720 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23322406 |
Subsequently, the ubiquitination of CARMA1 catalyzed by STUB1 was identified as Lys 27 linked, which is important for CARMA1 mediated NF-kappaB activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity
ubiquitination
|
CIB1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278721 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 33082516 |
All those were suggesting that CHIP promotes CIB1 ubiquitination via the Lys 48 residue of ubiquitin.|K10 and K65 were the Lysine (K) residues for CHIP mediated CIB1 degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
CTBP2 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278722 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23410750 |
Our data showed that CHIP depletion resulted in up-regulation of the steady-state level of CtBP2 ( Fig. 1 B) and that CHIP ubiquitinated CtBP2 for proteasomal degradation ( Fig. 3 A). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
AR |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278782 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27007160 |
Via this mechanism, CHIP ubiquitinates and degrades glucocorticoid receptor (GR), androgen receptor (AR), estrogen receptor (ER), ErbB2, and alpha-synuclein, only when bound to Hsp . |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
CFLAR |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278783 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23256568 |
Taken together, our data suggest that CHIP interacts with c-FLIP L in vivo and promotes the ubiquitination of c-FLIP L.|When we knocked down CHIP, c-FLIP L degradation was inhibited after treatment with 17-AAG, which indicated that CHIP modulated c-FLIP L degradation in the NSCLC cell lines. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
DDIAS |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278784 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28079882 |
HSP70 recruits DDIAS to the CHIP E3 ligase, whereas CHIP promotes the ubiquitination of DDIAS.|However, the findings clearly demonstrated that HSP70 was solely involved in CHIP mediated proteasomal degradation of DDIAS. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
SMAD3 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278785 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 24613385 |
These results suggest that Smad3 could be easily ubiquitinated and degraded by CHIP when Hsp90 activity was inhibited.To demonstrate the role of Hsp70 and Hsp90 on the regulation of Smad3 by CHIP, we over-expressed Hsp70 and Hsp90 in mammalian cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates
ubiquitination
|
SMAD1 |
0.332 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-172993 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21454478 |
In ad-dition, some proteins (e.g. Chip, carboxyl terminus of hsc70-interacting protein) inhibit the signaling activities of smad1/5 by recruiting smad1/5 from the functional r-/co-smad complex and further pro-moting the ubiquitination and degradation of smad1/5 in a chaperone-independent manner |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195687 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
In ad-dition, some proteins (e.g. Chip, carboxyl terminus of hsc70-interacting protein) inhibit the signaling activities of smad1/5 by recruiting smad1/5 from the functional r-/co-smad complex and further pro-moting the ubiquitination and degradation of smad1/5 in a chaperone-independent manner |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-120731 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14701756 |
These results suggest that chip can interact with the smad1/smad4 proteins and block bmp signal transduction through the ubiquitin-mediated degradation of smad proteins. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
destabilization
|
CD274 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274979 |
|
|
Homo sapiens |
A-375 Cell |
| pmid |
sentence |
| 28813410 |
Deletion of STUB1 resulted in a more profound increase in PD-L1 levels in CMTM6 deficient than in CMTM6 proficient cells, identifying STUB1 as an E3 ligase that causes destabilization of PD-L1 (Fig. 4f,g), either by direct modification of one of the lysines in the PD-L1 cytoplasmic domain or indirectly |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
HSPA8 |
0.731 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272588 |
|
|
Chlorocebus aethiops |
COS-1 Cell |
| pmid |
sentence |
| 11676916 |
BAG-1 stimulates CHIP-induced degradation of the glucocorticoid hormone receptor (GR). A model for the cooperation of CHIP and BAG-1 in coupling Hsc/Hsp70 to the ubiquitin/proteasome system. CHIP associates with Hsc/Hsp70 via its TPR chaperone adaptor (TPR) and, at the same time, recruits E2 ubiquitin-conjugating enzymes of the Ubc4/5 family to the chaperone complex. BAG-1 binds to Hsp70 via its BAG domain (BAG) and utilizes its ubiquitin-like domain (ubl) for proteasomal association |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
SMAD1 |
0.332 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272948 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 14701756 |
CHIP mediates the ubiquitination of Smad1. we demonstrate that the coexpression of Smad1 and Smad4 with the CHIP protein results in the degradation of the Smad proteins through a ubiquitin-mediated process. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
SMG5 |
0.406 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272649 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16809764 |
Here, we report that the human ortholog of the yeast ever-shorter telomeres 1B (EST1B) binds HDAC8. This interaction is regulated by protein kinase A-mediated HDAC8 phosphorylation and protects human EST1B (hEST1B) from ubiquitin-mediated degradation. Phosphorylated HDAC8 preferentially recruits Hsp70 to a complex that inhibits the CHIP (C-terminal heat shock protein interacting protein) E3 ligase-mediated degradation of hEST1B. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
ATCAY |
0.384 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272651 |
|
|
in vitro |
|
| pmid |
sentence |
| 16275660 |
CHIP e.g. was found to efficiently polyubiquitinate caytaxin in vitro, suggesting that it might influence caytaxin degradation in vivo. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
STUB1 | down-regulates
ubiquitination
|
SMAD5 |
0.348 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195690 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22298955 |
In ad-dition, some proteins (e.g. Chip, carboxyl terminus of hsc70-interacting protein) inhibit the signaling activi-ties of smad1/5 by recruiting smad1/5 from the functional r-/co-smad complex and further pro-moting the ubiquitination and degradation of smad1/5 in a chaperone-independent manne |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-172996 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21454478 |
In addition, some proteins (e.g. Chip, carboxyl terminus of hsc70-interacting protein) inhibit the signaling activities of smad1/5 by recruiting smad1/5 from the functional r/co-smad complex and further promoting the ubiquitination and degradation of smad1/5 in a chaperone-independent manner |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
S100A2 |
0.321 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272918 |
|
|
in vitro |
|
| pmid |
sentence |
| 23344957 |
S100 protein itself is ubiquitinated by CHIP in a Ca2+-dependent manner.Ubiquitylated S100 proteins are shown as (Ub)n-S100A2 and (Ub)n-S100P. The association of the S100 proteins with CHIP provides a Ca2+-dependent regulatory mechanism for the ubiquitination and degradation of intracellular proteins by the CHIP-proteasome pathway. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
S100P |
0.295 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272919 |
|
|
in vitro |
|
| pmid |
sentence |
| 23344957 |
S100 protein itself is ubiquitinated by CHIP in a Ca2+-dependent manner.Ubiquitylated S100 proteins are shown as (Ub)n-S100A2 and (Ub)n-S100P. The association of the S100 proteins with CHIP provides a Ca2+-dependent regulatory mechanism for the ubiquitination and degradation of intracellular proteins by the CHIP-proteasome pathway. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
HIF1A |
0.382 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271426 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19940151 |
the ubiquitin ligase activity of CHIP regulates HIF-1α degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | up-regulates activity
binding
|
PRKN |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272888 |
|
|
in vitro |
|
| pmid |
sentence |
| 12150907 |
In this study, we found that CHIP promotes Parkin-mediated Pael-R ubiquitination and subsequent degradation. In vitro ubiquitination assays suggested that only a combination of both Parkin and its cofactor CHIP function as a ubiquitin ligase, which is able to sufficiently ubiquitinate Pael-R in vivo (Figure 6). |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
BAG1 | up-regulates activity
binding
|
STUB1 |
0.553 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272587 |
|
|
Chlorocebus aethiops |
COS-1 Cell |
| pmid |
sentence |
| 11676916 |
BAG-1 stimulates CHIP-induced degradation of the glucocorticoid hormone receptor (GR). A model for the cooperation of CHIP and BAG-1 in coupling Hsc/Hsp70 to the ubiquitin/proteasome system. CHIP associates with Hsc/Hsp70 via its TPR chaperone adaptor (TPR) and, at the same time, recruits E2 ubiquitin-conjugating enzymes of the Ubc4/5 family to the chaperone complex. BAG-1 binds to Hsp70 via its BAG domain (BAG) and utilizes its ubiquitin-like domain (ubl) for proteasomal association |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
TAL1 |
0.361 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271393 |
|
|
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 17962192 |
Ubiquitination and degradation of Tal1/SCL are induced by notch signaling and depend on Skp2 and CHIP. CHIP promoted Tal1 degradation with both chaperone binding and ubiquitin ligase activities, which are mediated by its TPR domain and U box, respectively. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
CIP2A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272877 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 24293411 |
CHIP is the ubiquitin E3 ligase mediating celastrol-triggered CIP2A degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FBXO2 | up-regulates activity
binding
|
STUB1 |
0.576 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271589 |
|
|
Rattus norvegicus |
PC-12 Cell |
| pmid |
sentence |
| 16682404 |
Through this novel interaction, which is mediated by the TPR domain of CHIP and an N-terminal PEST domain of Fbx2, CHIP facilitates the ubiquitination and degradation of Fbx2-bound glycoproteins. This study highlights a novel mechanism of F-box protein-mediated ubiquitination that contributes to glycoprotein homeostasis. |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
PSMD4 |
0.429 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272752 |
|
|
in vitro |
|
| pmid |
sentence |
| 19240029 |
S5a/Rpn10 is a ubiquitin (Ub)-binding protein that is a subunit of the 26S proteasome but also exists free in the cytosol. It binds poly-Ub chains through its two Ub-interacting motifs (UIMs). We discovered that, unlike typical substrates of Ub ligases (E3s), S5a can be ubiquitinated by all E3s tested including multimeric and monomeric Ring finger E3s (MuRF1, Siah2, Parkin, APC, and SCF(betaTRCP1)), the U-box E3, CHIP, and HECT domain E3s (E6AP and Nedd4) when assayed with UbcH5 or related Ub-conjugating enzymes.The short half-life of S5a presumably is because of the presence of the UIM domain and reflects the ubiquitination of free S5a by many E3s. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
NRK |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274110 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32162334 |
Our results indicate that Nrk is ubiquitinated by CHIP in a chaperone-dependent manner, resulting in its proteasomal degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
IRS4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277616 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 30026872 |
IRS4 was phosphorylated at Ser859 by CK1γ2 in vitro and in vivo, which promoted the polyubiquitination and degradation of IRS4 through the ubiquitin/lysosome pathway by the carboxyl terminus of Hsc70-interacting protein(CHIP). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
polyubiquitination
|
SMAD4 |
0.403 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272947 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 14701756 |
We demonstrate that the coexpression of Smad1 and Smad4 with the CHIP protein results in the degradation of the Smad proteins through a ubiquitin-mediated process. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
PLK1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278532 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27390342 |
As indicated in xref , all three Plk1 fragments were ubiquitinated by CHIP, suggesting that CHIP targets multiple sites of Plk1 for ubiquitination.|Mechanistically, CHIP mediated degradation of AR and Plk1 leads to enhanced efficacy of HSP90 inhibitors. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STUB1 | down-regulates quantity by destabilization
ubiquitination
|
SNAI1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278545 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30927556 |
These results indicate that CHIP can ubiquitylate and degrade Snail in a GSK\u20103\u03b2\u2010independent manner.|These results suggest that CHIP negatively regulates the stability of Snail through inducing its proteasomal degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
STUB1
- 
- 