| + |
STUB1 | down-regulates quantity by destabilization 
ubiquitination
|
POU5F1 |
0.305 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278562 |
Lys284 |
WFCNRRQkGKRSSSD |
Homo sapiens |
|
| pmid |
sentence |
| 29511337 |
CHIP overexpression decreased OCT4 stability through proteasomal degradation.|CHIP E3 ligase ubiquitinates OCT4 at lysine 284.|These data suggest that CHIP induces OCT4 ubiquitination and degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPK1 | down-regulates
phosphorylation
|
POU5F1 |
0.384 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-192097 |
Ser111 |
ESNSDGAsPEPCTVT |
Homo sapiens |
|
| pmid |
sentence |
| 23024368 |
We demonstrate that oct4a interacts with erk1/2 by using both in vitro gst pulldown and in vivo co-immunoprecipitation assays. Ms analysis identified phosphorylation of oct4a at ser-111. / serine 111 phosphorylation regulates oct4a protein subcellular distribution and degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPK3 | down-regulates
phosphorylation
|
POU5F1 |
0.346 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-192101 |
Ser111 |
ESNSDGAsPEPCTVT |
Homo sapiens |
|
| pmid |
sentence |
| 23024368 |
Phosphorylation of this site downregulates nanog, sox2, rex1 and upregulates bmp4, gata6, ddlx5. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CyclinE/CDK2 | up-regulates quantity by stabilization 
phosphorylation
|
POU5F1 |
0.32 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264436 |
Ser12 |
LASDFAFsPPPGGGG |
in vitro |
|
| pmid |
sentence |
| 31306665 |
Recent work found that CyclinE/CDK2 can phosphorylate OCT4 on serine 12 (S12), serine 355 (S355) and threonine 322 (T322) in vitro|Phosphorylation of OCT4 on S12 has been previously implicated to stabilize OCT4 by binding to PIN1, thereby preventing ubiquitinylation by WWP2. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264437 |
Ser12 |
LASDFAFsPPPGGGG |
in vitro |
|
| pmid |
sentence |
| 31306665 |
Recently, Liu et al. [3] identified CyclinE/CDK2 to be the kinase phosphorylating OCT4 on serine 12 (S12), serine 355 (S355) and threonine 322 (T322) by elegantly combining genetics and biochemistry. Knockout of all five G1 cyclins (D1, D2, D3, E1 and E2) in mESCs (coined Q-KO cells) and consequent inactivation of CDK2/4/ 6 leads to perturbation of the pluripotent state and to the adaption of the trophectoderm cell fate. This was attributed to reduced phosphorylation of OCT4 (as well as SOX2 and NANOG) leading to an increase of protein turnover [3]. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264438 |
Ser12 |
LASDFAFsPPPGGGG |
in vitro |
|
| pmid |
sentence |
| 31306665 |
Recently, Liu et al. [3] identified CyclinE/CDK2 to be the kinase phosphorylating OCT4 on serine 12 (S12), serine 355 (S355) and threonine 322 (T322) by elegantly combining genetics and biochemistry. Knockout of all five G1 cyclins (D1, D2, D3, E1 and E2) in mESCs (coined Q-KO cells) and consequent inactivation of CDK2/4/ 6 leads to perturbation of the pluripotent state and to the adaption of the trophectoderm cell fate. This was attributed to reduced phosphorylation of OCT4 (as well as SOX2 and NANOG) leading to an increase of protein turnover [3]. |
|
| Publications: |
3 |
Organism: |
In Vitro |
| + |
CDK7 | up-regulates quantity by stabilization
phosphorylation
|
POU5F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264404 |
Ser12 |
LASDFAFsPPPGGGG |
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 31306665 |
Here, we combined molecular and cellular biology with CRISPR/Cas9-mediated genome engineering to pinpoint the function of serine 12 of OCT4 in ESCs. Using chemical inhibitors and an antibody specific to OCT4 phosphorylated on S12, we identified cyclin-dependent kinase (CDK) 7 as upstream kinase. |Phosphorylation of OCT4 on S12 has been previously implicated to stabilize OCT4 by binding to PIN1, thereby preventing ubiquitinylation by WWP2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKT3 | up-regulates quantity by stabilization
phosphorylation
|
POU5F1 |
0.258 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242107 |
Thr235 |
QARKRKRtSIENRVR |
Homo sapiens |
NCCIT Cell |
| pmid |
sentence |
| 23041284 |
Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine 235, and that the levels of phosphorylated Oct4 in ECCs correlated with resistance to apoptosis and tumorigenic potential. Phosphorylation of Oct4 increased its stability and facilitated its nuclear localization and its interaction with Sox2, which promoted the transcription of the core stemness genes POU5F1 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKT | up-regulates quantity by stabilization
phosphorylation
|
POU5F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242092 |
Thr235 |
QARKRKRtSIENRVR |
Homo sapiens |
NCCIT Cell |
| pmid |
sentence |
| 23041284 |
Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine 235, and that the levels of phosphorylated Oct4 in ECCs correlated with resistance to apoptosis and tumorigenic potential. Phosphorylation of Oct4 increased its stability and facilitated its nuclear localization and its interaction with Sox2, which promoted the transcription of the core stemness genes POU5F1 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKT1 | up-regulates quantity by stabilization
phosphorylation
|
POU5F1 |
0.462 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252545 |
Thr235 |
QARKRKRtSIENRVR |
Homo sapiens |
NCCIT Cell |
| pmid |
sentence |
| 23041284 |
Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine 235, and that the levels of phosphorylated Oct4 in ECCs correlated with resistance to apoptosis and tumorigenic potential. Phosphorylation of Oct4 increased its stability and facilitated its nuclear localization and its interaction with Sox2, which promoted the transcription of the core stemness genes POU5F1 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKT2 | up-regulates quantity by stabilization
phosphorylation
|
POU5F1 |
0.256 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242097 |
Thr235 |
QARKRKRtSIENRVR |
Homo sapiens |
NCCIT Cell |
| pmid |
sentence |
| 23041284 |
Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine 235, and that the levels of phosphorylated Oct4 in ECCs correlated with resistance to apoptosis and tumorigenic potential. Phosphorylation of Oct4 increased its stability and facilitated its nuclear localization and its interaction with Sox2, which promoted the transcription of the core stemness genes POU5F1 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression 
transcriptional regulation
|
PAX6 |
0.459 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253163 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CTNNB1 | up-regulates activity
binding
|
POU5F1 |
0.579 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-241981 |
|
|
Mus musculus |
Embryonic Stem Cell |
| pmid |
sentence |
| 21295277 |
We provide evidence suggesting that Beta-catenins interaction with the pluripotency regulator Oct-4 at least partially underlies its effects on sustaining pluripotency. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
POU5F1 | up-regulates quantity by expression 
transcriptional regulation
|
THY1 |
0.425 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254931 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | form complex 
binding
|
SOX2/POU5F1 |
0.84 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-29509 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 7590241 |
Sox2 can form a ternary complex with either the ubiquitous oct-1 or the embryonic-specific oct-3 protein on fgf-4 enhancer dna sequences. However, only the sox2/oct-3 complex is able to promote transcriptional activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRIM32 | down-regulates quantity by destabilization
ubiquitination
|
POU5F1 |
0.269 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278620 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 26307407 |
This further supports that TRIM32 and Oct4 do physically interact, so that TRIM32 can specifically ubiquitinate Oct4 and thereby target it for degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
LEFTY1 |
0.504 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254938 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
CDKN1A |
0.405 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255043 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 19968627 |
p21 protein levels were repressed by Oct-4 and were induced by the down-regulation of Oct-4 in ZHBTc4 ES cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
AKT1 |
0.462 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252635 |
|
|
Homo sapiens |
NCCIT Cell |
| pmid |
sentence |
| 23041284 |
Furthermore, in ECCs, unphosphorylated Oct4 bound to the AKT1 promoter and repressed its transcription. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
SOX2 |
0.84 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254942 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates
|
Pluripotency |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242070 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 16153702 |
Our results suggest that OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal by activating their own genes and genes encoding components of key signaling pathways and by repressing genes that are key to developmental processes. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242067 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 25126380 |
Sex determining region Y-box 2 (Sox2), a member of the SoxB1 transcription factor family, is an important transcriptional regulator in pluripotent stem cells (PSCs). Together with octamer-binding transcription factor 4 and Nanog, they co-operatively control gene expression in PSCs and maintain their pluripotency. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
DPPA4 |
0.628 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254941 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
DLX5 |
0.311 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254935 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
GATA6 |
0.483 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253159 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
LEFTY2 |
0.423 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254940 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
TBX18 |
0.325 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254945 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity
|
RARG |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269222 |
|
|
|
|
| pmid |
sentence |
| 27499297 |
OCT4 positively controls the level of RARgamma |
|
| Publications: |
1 |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
TBXT |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254929 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.769 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254939 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
BMP4 |
0.476 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254932 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ETV4 | up-regulates quantity by expression
transcriptional regulation
|
POU5F1 |
0.381 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-205173 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 24983502 |
Transcriptional activation of oct4 by the ets transcription factor pea3 in nccit human embryonic carcinoma cells |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | form complex
binding
|
SOX17/POU5F1 |
0.613 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269221 |
|
|
|
|
| pmid |
sentence |
| 23474895 |
Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm|We show that Sox17 partners with Oct4 and binds to a unique ‘compressed' Sox/Oct motif that earmarks endodermal genes. This is in contrast to the pluripotent state where Oct4 selectively partners with Sox2 at ‘canonical' binding sites. |
|
| Publications: |
1 |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
TDGF1 |
0.688 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254943 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
EOMES |
0.426 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254930 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
CDX2 |
0.565 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254933 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
WWP2 | down-regulates quantity
ubiquitination
|
POU5F1 |
0.455 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268850 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19274063 |
WWP2 promotes degradation of transcription factor OCT4 in human embryonic stem cells|Here, we report that human WWP2, an E3 ubiquitin (Ub)-protein ligase, interacts with OCT4 specifically through its WW domain and enhances Ub modification of OCT4 both in vitro and in vivo. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ERK1/2 | down-regulates
phosphorylation
|
POU5F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270185 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23024368 |
Phosphorylation of this site downregulates nanog, sox2, rex1 and upregulates bmp4, gata6, ddlx5. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZNF322 | up-regulates quantity by expression
transcriptional regulation
|
POU5F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264900 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 24550733 |
Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays revealed that Zfp322a binds to Pou5f1 and Nanog promoters and regulates their transcription. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
ZFP42 |
0.469 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254944 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
HLX |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254936 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PRDM14 | up-regulates quantity by expression
transcriptional regulation
|
POU5F1 |
0.623 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255067 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 20953172 |
We showed that PRDM14 regulates directly the expression of key pluripotency gene POU5F1 through its proximal enhancer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
DKK1 |
0.352 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254934 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SOX2/POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
POU5F1 |
0.84 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269254 |
|
|
|
|
| pmid |
sentence |
| 31583686 |
Both SOX2 and SOX17 are able to partner with OCT4 and, as a consequence, recognize and bind specific binding motifs.6, 7 In human and mouse ESCs, SOX2/OCT4 bind to canonical motifs (CTTTGTCATGCAAAT-like), which are composite SOX (CATTGTC-like) and OCT (ATGCAAAT-like) motifs|This way SOX17 and SOX2 regulate a common set of pluripotency and GC-related genes (PRDM14, DPPA4, TDGF1, NANOG, LIN28A, TRIM71, OTX2, PIM2) (Fig. 6). Additionally, in TCam-2 cells SOX17 binds to compressed motifs or SOX motifs (not bound by SOX2 in ECs), thereby regulating the PGC specifiers PRDM1 and TFAP2C, the GC-related genes NANOS3 and BMP7 and the cancer-related genes MYC and IGF1 (Fig. 6). In 2102EP cells, SOX2 further binds canonical elements or SOX motifs (not bound by SOX17 in TCam-2), regulating additional pluripotency genes (GDF3, LEFTY2, SALL4, SOX2 and POU5F1) (Fig. 6). |
|
| Publications: |
1 |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
SOX17 |
0.613 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253167 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Av/b1 integrin | up-regulates quantity by expression
|
POU5F1 |
0.336 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253272 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 18757303 |
Recombinant human LN-511 alone was suffi- cient to enable self-renewal of mouse ES cells for up to 169 days (31 passages). Cells cultured on LN-511 maintained expression of pluripotency markers, such as Oct4, Sox2, Tert, UTF1, and Nanog|ES cells interacted with LN-511 via 1-integrins, mostly a6b1 and aVb1. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
GATA4 |
0.502 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253161 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
FOXA2 |
0.43 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253165 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
ID2 |
0.29 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254937 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Gbeta | down-regulates
phosphorylation
|
POU5F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270085 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23024368 |
Phosphorylation of this site downregulates nanog, sox2, rex1 and upregulates bmp4, gata6, ddlx5. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
A6/b1 integrin | up-regulates quantity by expression
|
POU5F1 |
0.391 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253278 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 18757303 |
Recombinant human LN-511 alone was suffi- cient to enable self-renewal of mouse ES cells for up to 169 days (31 passages). Cells cultured on LN-511 maintained expression of pluripotency markers, such as Oct4, Sox2, Tert, UTF1, and Nanog|ES cells interacted with LN-511 via 1-integrins, mostly a6b1 and aVb1. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
POU5F1 | down-regulates quantity by repression
transcriptional regulation
|
AKT |
0.462 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242103 |
|
|
Homo sapiens |
NCCIT Cell |
| pmid |
sentence |
| 23041284 |
Furthermore, in ECCs, unphosphorylated Oct4 bound to the AKT1 promoter and repressed its transcription. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
DNMT1 |
0.441 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253158 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22795133 |
Oct4 and Nanog upregulate Dnmt1 through direct binding to its promoter, thereby leading to the repressed expression of p16 and p21 and genes associated with development and lineage differentiation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
POU5F1
- 
- 