| + |
AKT1 | down-regulates activity 
phosphorylation
|
BECN1 |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279666 |
Ser234 |
AQYQREYsEFKRQQL |
Homo sapiens |
|
| pmid |
sentence |
| 31852899 |
In addition, pharmacological inhibition of AKT1 enhanced BECN1 stability in both assays, leaving about twice the amount of BECN1 at 90 min compared to control (Fig. 4i\u2013l).|The oncogenic kinase AKT1 phosphorylates BECN1 at positions S234, S295, which leads to sequestration of this peripheral membrane binding protein xref to the cytoskeleton with the result of inhibition of autophagy xref . |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279667 |
Ser295 |
FRLGRLPsVPVEWNE |
Homo sapiens |
|
| pmid |
sentence |
| 31852899 |
In addition, pharmacological inhibition of AKT1 enhanced BECN1 stability in both assays, leaving about twice the amount of BECN1 at 90 min compared to control (Fig. 4i\u2013l).|The oncogenic kinase AKT1 phosphorylates BECN1 at positions S234, S295, which leads to sequestration of this peripheral membrane binding protein7 to the cytoskeleton with the result of inhibition of autophagy8. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK3 | up-regulates activity 
phosphorylation
|
BECN1 |
0.425 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279342 |
Ser90 |
IPPARMMsTESANSF |
Homo sapiens |
|
| pmid |
sentence |
| 25693418 |
Taken together, these data indicate that MK2 and MK3 directly phosphorylate Beclin 1 S90 in vitro, and that MK2 like kinase activity also mediates starvation induced Beclin 1 S90 phosphorylation in cultured cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
BECN1 |
0.432 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278342 |
Ser90 |
IPPARMMsTESANSF |
Homo sapiens |
|
| pmid |
sentence |
| 25693418 |
Beclin 1 S90 is phosphorylated by MAPKAPK2 (MK2) and MAPKAPK3 (MK3). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STYK1 | up-regulates activity
phosphorylation
|
BECN1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264568 |
Ser90 |
IPPARMMsTESANSF |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 31696776 |
We also demonstrated that STYK1 elevated the serine phosphorylation of BECN1, thereby decreasing the interaction between BECN1 and BCL2. |The results indicated that the level of BECN1 S90 phosphorylation significantly increased after STYK1 overexpression, but not STYK1K147R mutant.|STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CHEK2 | up-regulates activity
phosphorylation
|
BECN1 |
0.299 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264557 |
Ser90 |
IPPARMMsTESANSF |
Homo sapiens |
NCI-H1299 Cell |
| pmid |
sentence |
| 32187724 |
We report that CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, thereby impairing Beclin 1-Bcl-2 autophagy-regulatory complex formation in a ROS-dependent fashion.|CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, promoting autophagy via Beclin 1 release from Bcl‐2 sequestration |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264556 |
Ser93 |
ARMMSTEsANSFTLI |
Homo sapiens |
NCI-H1299 Cell |
| pmid |
sentence |
| 32187724 |
We report that CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, thereby impairing Beclin 1-Bcl-2 autophagy-regulatory complex formation in a ROS-dependent fashion.|CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, promoting autophagy via Beclin 1 release from Bcl‐2 sequestration |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
STK4 | up-regulates activity
phosphorylation
|
BECN1 |
0.312 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278502 |
Thr108 |
GEASDGGtMENLSRR |
Homo sapiens |
|
| pmid |
sentence |
| 24141421 |
These results suggest that Mst1 directly stimulates interaction between Beclin1 and Bcl-2.|We here demonstrate that Mst1-induced phosphorylation of Beclin1 in its BH3 domain at Thr 108 promotes binding of Beclin1 with Bcl-2/Bcl-xL. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DAPK1 | up-regulates
phosphorylation
|
BECN1 |
0.726 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-185589 |
Thr119 |
LSRRLKVtGDLFDIM |
Homo sapiens |
|
| pmid |
sentence |
| 19395874 |
We found that DAPk phosphorylates Beclin 1 on T119, a critical residue within its BH3 domain, and thus promotes Beclin 1 dissociation from Bcl-X(L) and autophagy induction. Here we report that T119 phosphorylation also reduces the interaction between Beclin 1 and Bcl-2, in line with the high degree of structural homology between the BH3 binding pockets of Bcl-2 and Bcl-X(L) proteins. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-183548 |
Thr119 |
LSRRLKVtGDLFDIM |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19180116 |
The activated form of DAPK triggers autophagy in a beclin-1-dependent manner. DAPK phosphorylates beclin 1 on Thr 119 located at a crucial position within its BH3 domain, and thus promotes the dissociation of beclin 1 from Bcl-XL and the induction of autophagy. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
ROCK1 | up-regulates activity
phosphorylation
|
BECN1 |
0.414 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278198 |
Thr119 |
LSRRLKVtGDLFDIM |
Homo sapiens |
|
| pmid |
sentence |
| 23877263 |
Beclin1 is phosphorylated by ROCK1 at T119. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
EGFR | down-regulates activity
phosphorylation
|
BECN1 |
0.521 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278357 |
Tyr233 |
EAQYQREySEFKRQQ |
Homo sapiens |
|
| pmid |
sentence |
| 24034250 |
The mechanism by which EGFR suppresses Beclin 1 function involves EGFR interaction with two domains (BH3 and ECD) of Beclin 1; EGFR mediated multisite tyrosine phosphorylation of Beclin 1 on residues Y229, Y233 and Y352; and EGFR mediated alterations in the Beclin 1 interactome (increased binding to the negative regulators, Bcl-2 and Rubicon, and decreased binding to the VPS34 lipid kinase).|Thus, EGFR signaling suppresses autophagy via its interaction with Beclin 1 during normal mitogenic signaling as well as during aberrant cell proliferation in cancer cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
JAK2 | up-regulates quantity
phosphorylation
|
BECN1 |
0.297 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278312 |
Tyr333 |
QRYRLVPyGNHSYLE |
Homo sapiens |
|
| pmid |
sentence |
| 34131122 |
Importantly, JAK2 knockdown significantly abolished the increased levels of p-BECN1 (Y333) induced by IL-6 stimulation in both SW48 and LoVo cells (Fig. 3k and Supplementary Fig. 4f).|Taken together, these data indicate that JAK2 specifically phosphorylates BECN1 at Y333. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
JAK2 | up-regulates activity
phosphorylation
|
BECN1 |
0.297 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277567 |
Tyr333 |
QRYRLVPyGNHSYLE |
Homo sapiens |
SW-48 Cell |
| pmid |
sentence |
| 34131122 |
Mechanistically, IL-6 triggers the interaction between JAK2 and BECN1, where JAK2 phosphorylates BECN1 at Y333. We demonstrate that BECN1 Y333 phosphorylation is crucial for BECN1 activation and IL-6-induced autophagy by regulating PI3KC3 complex formation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ACSS2 | up-regulates quantity by expression
transcriptional regulation
|
BECN1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276561 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
Cullin 3-RBX1-Skp1 | down-regulates quantity by destabilization
polyubiquitination
|
BECN1 |
0.33 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272419 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 26687681 |
Cul3-KLHL20 Ubiquitin Ligase Governs the Turnover of ULK1 and VPS34 Complexes to Control Autophagy Termination. KLHL20 promotes ubiquitination of phagophore-residing VPS34 and Beclin-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BECN1 | form complex 
binding
|
Vps34 Complex I |
0.936 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260315 |
|
|
in vitro |
|
| pmid |
sentence |
| 30397185 |
PtdIns3P recruits specific recognition modules that are common in protein-sorting pathways, such as autophagy and endocytic sorting. It is best characterized in two heterotetramers, complexes I and II. Complex I is composed of VPS34, VPS15, Beclin 1, and autophagy-related gene (ATG)14L, whereas complex II replaces ATG14L with UVRAG. |Complexes I and II are critical for early events in autophagy and endocytic sorting, respectively. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| Pathways: | Autophagy |
| + |
BECN1 | up-regulates quantity by stabilization
deubiquitination
|
USP10 |
0.508 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260298 |
|
|
Homo sapiens |
H4 Neuroglioma Cell |
| pmid |
sentence |
| 21962518 |
Interestingly, Beclin1 also controls the protein stabilities of USP10 and USP13 by regulating their deubiquitinating activities. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BCL2L1 | down-regulates
binding
|
BECN1 |
0.915 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-154480 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17446862 |
The anti-apoptotic proteins bcl-2 and bcl-x(l) bind and inhibit beclin-1, an essential mediator of autophagy. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PIK3C3 | up-regulates activity
binding
|
BECN1 |
0.936 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-184521 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 19270693 |
The beclin 1-vps34 interaction regulates autophagy. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Autophagy |
| + |
UVRAG | up-regulates activity
binding
|
BECN1 |
0.86 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-150825 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 17106237 |
UVRAG interacts with Beclin 1, leading to activation of autophagy and thereof inhibition of tumorigenesis. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-171902 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21311563 |
Beclin 1, the mammalian orthologue of yeast Atg6, has a central role in autophagy, a process of programmed cell survival, which is increased during periods of cell stress and extinguished during the cell cycle. It interacts with several cofactors (Atg14L, UVRAG, Bif-1, Rubicon, Ambra1, HMGB1, nPIST, VMP1, SLAM, IP(3)R, PINK and survivin) to regulate the lipid kinase Vps-34 protein and promote formation of Beclin 1-Vps34-Vps15 core complexes |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Autophagy |
| + |
GOPC | up-regulates
binding
|
BECN1 |
0.524 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-171896 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21311563 |
Npist binds beclin 1 by a ccd |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AMBRA1 | up-regulates activity
binding
|
BECN1 |
0.785 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-168252 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20921139 |
we show that the BECLIN 1-VPS34 complex is tethered to the cytoskeleton through an interaction between the BECLIN 1-interacting protein AMBRA1 and dynein light chains 1/2. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-156409 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17589504 |
Here we show that Ambra1 (activating molecule in Beclin1-regulated autophagy), a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. We found that Ambra1 is a positive regulator of the Becn1-dependent programme of autophagy |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Tissue: |
Fibrosarcoma Cell |
| Pathways: | Autophagy |
| + |
BECN1 | up-regulates activity
binding
|
ZWINT |
0.436 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265027 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 23478334 |
We show that Beclin-1 interacts directly with Zwint-1-a component of the KMN (KNL-1/Mis12/Ndc80) complex-which is essential for kinetochore-microtubule interactions. This suggests that Beclin-1 acts downstream of the KMN complex to influence the recruitment of outer kinetochore proteins and promotes accurate kinetochore anchoring to the spindle during mitosis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BCL2 | down-regulates
binding
|
BECN1 |
0.739 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-156941 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17643073 |
In mammalian cells, the antiapoptotic protein, bcl-2, binds to beclin 1 during nonstarvation conditions and inhibits its autophagy function. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-179084 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18570871 |
In mammalian cells, the antiapoptotic protein, bcl-2, binds to beclin 1 during nonstarvation conditions and inhibits its autophagy function. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-154477 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17446862 |
In mammalian cells, the antiapoptotic protein, bcl-2, binds to beclin 1 during nonstarvation conditions and inhibits its autophagy function. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| Pathways: | Autophagy |
| + |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
BECN1 |
0.409 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276558 |
|
|
|
|
| pmid |
sentence |
| 28552616 |
As expected, we found that glucose deprivation induced the binding of TFEB (Figure S4C) and ACSS2 (Figure S4D) to the promoter regions of MAP1LC3B, ATG3, and WIPI-1 as well as mRNA (Figure 3H) and protein (Figure 3I) expression of these genes; |
|
| Publications: |
1 |
| + |
NEDD4 | up-regulates quantity by stabilization
ubiquitination
|
BECN1 |
0.461 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278558 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 29251248 |
BECN1 stability was increased by NEDD4 via K6 and K27 ubiquitination during autophagy induction, thereby promoting autophagy activation.|Further, NEDD4 mediated K6- and K27- linkage ubiquitination of BECN1, leading to elevated stability of BECN1 and increased autophagy. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SH3GLB1 | up-regulates
binding
|
BECN1 |
0.543 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-171899 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21311563 |
Bif-1 forms a complex with beclin1 through uvrag and promotes the activation of the class iii pi3 kinase, vps34, in mammalian cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ULK1 | up-regulates activity
phosphorylation
|
BECN1 |
0.77 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278503 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 29671878 |
In the nucleation step of autophagy, The ULK1 complex phosphorylates and activates the Beclin-1-VPS34 complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SIRT5 | up-regulates quantity by expression 
transcriptional regulation
|
BECN1 |
0.315 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261206 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 31608237 |
In SIRT5 silenced Mero-14 cells, beclin-1 protein levels were downregulated upon rotenone and resveratrol treatment. In summary, in most cases, where significant effects were detected, SIRT1, SIRT3, and SIRT5 had positive effects on beclin1 and LC3II/LC3I ratio |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BECN1 | up-regulates quantity by stabilization
deubiquitination
|
USP13 |
0.528 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260296 |
|
|
Homo sapiens |
H4 Neuroglioma Cell |
| pmid |
sentence |
| 21962518 |
We found that endogenous Beclin1 can interact with USP13 and the interaction was reduced in the presence of spautin-1 (Figure 5C). Interestingly, the DUB activities were significantly increased when USP13 and USP10 coincubated together or with Beclin1 or all 3 proteins together, suggesting the DUB activity can be significantly enhanced when USP13 interacts with its substrate Beclin1 or USP10. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ADNP | up-regulates quantity by expression
transcriptional regulation
|
BECN1 |
0.249 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266760 |
|
|
Rattus norvegicus |
|
| pmid |
sentence |
| 24365867 |
Here, we show for the first time that hippocampal ADNP deficiency paralleled reduced beclin1 expression which, in turn, parallels increased tauopathy and cell death. We now show that ADNP directly interacts with LC3B, implicating the requirement of a healthy ADNP system for the apoptotic/autophagy processes. |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| + |
BECN1 | form complex
binding
|
Vps34 Complex II |
0.906 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260319 |
|
|
in vitro |
|
| pmid |
sentence |
| 30397185 |
PtdIns3P recruits specific recognition modules that are common in protein-sorting pathways, such as autophagy and endocytic sorting. It is best characterized in two heterotetramers, complexes I and II. Complex I is composed of VPS34, VPS15, Beclin 1, and autophagy-related gene (ATG)14L, whereas complex II replaces ATG14L with UVRAG. |Complexes I and II are critical for early events in autophagy and endocytic sorting, respectively. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| Pathways: | Autophagy |
| + |
BECN1 | up-regulates
|
Autophagosome_formation |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-219545 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20921139 |
Autophagy initiation signaling requires both the ULK1 kinase and the BECLIN 1VPS34 core complex to generate autophagosomes, double-membraned vesicles that transfer cellular contents to lysosomes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Fibroma Cell |
| Pathways: | Autophagy |
| + |
KLHL20 | down-regulates quantity by destabilization
binding
|
BECN1 |
0.277 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272415 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 26687681 |
Cul3-KLHL20 Ubiquitin Ligase Governs the Turnover of ULK1 and VPS34 Complexes to Control Autophagy Termination. KLHL20 promotes ubiquitination of phagophore-residing VPS34 and Beclin-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
USP13 | up-regulates quantity by stabilization
deubiquitination
|
BECN1 |
0.528 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260295 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 21962518 |
Similarly, the overexpression of USP13 reduced the levels of ubiquitinated Beclin1 which was inhibited by spautin-1 (Figure 4E) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
USP10 | up-regulates quantity by stabilization
deubiquitination
|
BECN1 |
0.508 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260299 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 21962518 |
Similarly, the overexpression of USP13 reduced the levels of ubiquitinated Beclin1 which was inhibited by spautin-1 (Figure 4E) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
BECN1
- 
- 