| + |
DYRK2 | down-regulates quantity by destabilization 
phosphorylation
|
CDC25A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276738 |
Ser107 |
NPMRRIHsLPQKLLG |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276740 |
Ser156 |
KKPVRPVsRGCLHSH |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276736 |
Ser18 |
RRLLFACsPPPASQP |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276739 |
Ser185 |
SAPARMLsSNERDSS |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276734 |
Ser320 |
HQSLSLAsSPKGTIE |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276735 |
Ser321 |
QSLSLASsPKGTIEN |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276737 |
Ser88 |
DSGFCLDsPGPLDSK |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34363019 |
Here we describe a novel ubiquitin/proteasome-mediated pathway negatively regulating CDC25A stability, dependent on its phosphorylation by the serine/threonine kinase DYRK2. DYRK2 phosphorylates CDC25A on at least 7 residues, resulting in its degradation independent of the known CDC25A E3 ubiquitin ligases. |
|
| Publications: |
7 |
Organism: |
Homo Sapiens |
| + |
CDK1 | up-regulates 
phosphorylation
|
CDC25A |
0.836 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-95256 |
Ser116 |
PQKLLGCsPALKRSH |
Homo sapiens |
|
| pmid |
sentence |
| 12411508 |
Mitotic stabilization of cdc25a reflects its phosphorylation on ser17 and ser115 by cyclin b-cdk1, modifications required to uncouple cdc25a from its ubiquitin-proteasome-mediated turnover. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-95260 |
Ser18 |
RRLLFACsPPPASQP |
Homo sapiens |
|
| pmid |
sentence |
| 12411508 |
Mitotic stabilization of cdc25a reflects its phosphorylation on ser17 and ser115 by cyclin b-cdk1, modifications required to uncouple cdc25a from its ubiquitin-proteasome-mediated turnover. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CyclinB/CDK1 | up-regulates
phosphorylation
|
CDC25A |
0.842 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-216761 |
Ser116 |
PQKLLGCsPALKRSH |
Homo sapiens |
|
| pmid |
sentence |
| 12411508 |
Mitotic stabilization of cdc25a reflects its phosphorylation on ser17 and ser115 by cyclin b-cdk1, modifications required to uncouple cdc25a from its ubiquitin-proteasome-mediated turnover. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G2/M phase transition |
| + |
CHEK2 | down-regulates quantity by destabilization
phosphorylation
|
CDC25A |
0.836 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260778 |
Ser124 |
PALKRSHsDSLDHDI |
Homo sapiens |
|
| pmid |
sentence |
| 11298456 |
We conclude that Chk2-dependent phosphorylation of Cdc25A on Ser 123 represents a critical step in promoting its rapid destruction in response to IR-induced DNA damage. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-99721 |
Ser124 |
PALKRSHsDSLDHDI |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
We show that ir-induced destruction of cdc25a requires both atm and the chk2-mediated phosphorylation of cdc25a on serine 123. the basal turnover of cdc25a operating in unperturbed s phase required chk1-dependent phosphorylation of serines 123, 178, 278, and 292. Ir-induced acceleration of cdc25a proteolysis correlated with increased phosphate incorporation into these residues generated by a combined action of chk1 and chk2 kinases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-106808 |
Ser124 |
PALKRSHsDSLDHDI |
Homo sapiens |
|
| pmid |
sentence |
| 11298456 |
We show that IR-induced destruction of Cdc25A requires both ATM and the Chk2-mediated phosphorylation of Cdc25A on serine 123. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-99725 |
Ser178 |
LFTQRQNsAPARMLS |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
We show that ir-induced destruction of cdc25a requires both atm and the chk2-mediated phosphorylation of cdc25a on serine 123. the basal turnover of cdc25a operating in unperturbed s phase required chk1-dependent phosphorylation of serines 123, 178, 278, and 292. Ir-induced acceleration of cdc25a proteolysis correlated with increased phosphate incorporation into these residues generated by a combined action of chk1 and chk2 kinases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-118759 |
Ser178 |
LFTQRQNsAPARMLS |
Homo sapiens |
|
| pmid |
sentence |
| 14559997 |
The order and fidelity of cell cycle events in mammals is intimately linked to the integrity of the Chk1 kinase-Cdc25A phosphatase pathway. Chk1 phosphorylation targets Cdc25A for destruction and, as shown here, inhibits interactions between Cdc25A and its mitotic substrate cyclin B1-Cdk1. Phosphorylation of Cdc25A on serine 178 and threonine 507 facilitates 14-3-3 binding, and Chk1 phosphorylates both residues in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-99729 |
Ser279 |
VLKRPERsQEESPPG |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
We show that ir-induced destruction of cdc25a requires both atm and the chk2-mediated phosphorylation of cdc25a on serine 123. the basal turnover of cdc25a operating in unperturbed s phase required chk1-dependent phosphorylation of serines 123, 178, 278, and 292. Ir-induced acceleration of cdc25a proteolysis correlated with increased phosphate incorporation into these residues generated by a combined action of chk1 and chk2 kinases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-99733 |
Ser293 |
GSTKRRKsMSGASPK |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
We show that ir-induced destruction of cdc25a requires both atm and the chk2-mediated phosphorylation of cdc25a on serine 123. the basal turnover of cdc25a operating in unperturbed s phase required chk1-dependent phosphorylation of serines 123, 178, 278, and 292. Ir-induced acceleration of cdc25a proteolysis correlated with increased phosphate incorporation into these residues generated by a combined action of chk1 and chk2 kinases. |
|
| Publications: |
7 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CHEK2 | down-regulates quantity
phosphorylation
|
CDC25A |
0.836 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260835 |
Ser124 |
PALKRSHsDSLDHDI |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
Chk2 phosphorylates a subset of the Chk1-targeted sites of Cdc25A | Phosphorylation of serines 123, 178, 278, and 292 regulates both basal and IR-induced accelerated proteolysis of Cdc25A |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260833 |
Ser178 |
LFTQRQNsAPARMLS |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
Chk2 phosphorylates a subset of the Chk1-targeted sites of Cdc25A | Phosphorylation of serines 123, 178, 278, and 292 regulates both basal and IR-induced accelerated proteolysis of Cdc25A |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260834 |
Ser279 |
VLKRPERsQEESPPG |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
Chk2 phosphorylates a subset of the Chk1-targeted sites of Cdc25A | Phosphorylation of serines 123, 178, 278, and 292 regulates both basal and IR-induced accelerated proteolysis of Cdc25A |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260836 |
Ser293 |
GSTKRRKsMSGASPK |
Homo sapiens |
|
| pmid |
sentence |
| 12676583 |
Chk2 phosphorylates a subset of the Chk1-targeted sites of Cdc25A | Phosphorylation of serines 123, 178, 278, and 292 regulates both basal and IR-induced accelerated proteolysis of Cdc25A |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CHEK1 | down-regulates
phosphorylation
|
CDC25A |
0.851 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163134 |
Ser124 |
PALKRSHsDSLDHDI |
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
The signal for ubiquitination after uv and ir exposure is created by phosphorylation of cdc25a mediated by chk1 and chk2, respectively. Chk1 is a major kinase phosphorylating cdc25a (ser76/124) and cdc25c (ser216). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163138 |
Ser178 |
LFTQRQNsAPARMLS |
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
The signal for ubiquitination after uv and ir exposure is created by phosphorylation of cdc25a mediated by chk1 and chk2, respectively. Chk1 is a major kinase phosphorylating cdc25a (ser76/124) and cdc25c (ser216). regulation;btrc(induces);14-3-3 beta(induces);apoptosis, altered;14-3-3 beta(induces);ccna1(disrupts);cdk2(disrupts);cdk1(disrupts);ccnb1(disrupts); |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163142 |
Ser279 |
VLKRPERsQEESPPG |
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
The signal for ubiquitination after uv and ir exposure is created by phosphorylation of cdc25a mediated by chk1 and chk2, respectively. Chk1 is a major kinase phosphorylating cdc25a (ser76/124) and cdc25c (ser216). regulation;btrc(induces);14-3-3 beta(induces);apoptosis, altered;14-3-3 beta(induces);ccna1(disrupts);cdk2(disrupts);cdk1(disrupts);ccnb1(disrupts); |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163146 |
Ser293 |
GSTKRRKsMSGASPK |
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
The signal for ubiquitination after uv and ir exposure is created by phosphorylation of cdc25a mediated by chk1 and chk2, respectively. Chk1 is a major kinase phosphorylating cdc25a (ser76/124) and cdc25c (ser216). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163150 |
Ser76 |
SNLQRMGsSESTDSG |
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
The signal for ubiquitination after uv and ir exposure is created by phosphorylation of cdc25a mediated by chk1 and chk2, respectively. Chk1 is a major kinase phosphorylating cdc25a (ser76/124) and cdc25c (ser216). regulation;btrc(induces);14-3-3 beta(induces);apoptosis, altered;14-3-3 beta(induces);ccna1(disrupts);cdk2(disrupts);cdk1(disrupts);ccnb1(disrupts); |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163154 |
Thr507 |
KFRTKSRtWAGEKSK |
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
The signal for ubiquitination after uv and ir exposure is created by phosphorylation of cdc25a mediated by chk1 and chk2, respectively. Chk1 is a major kinase phosphorylating cdc25a (ser76/124) and cdc25c (ser216). regulation;btrc(induces);14-3-3 beta(induces);apoptosis, altered;14-3-3 beta(induces);ccna1(disrupts);cdk2(disrupts);cdk1(disrupts);ccnb1(disrupts); |
|
| Publications: |
6 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G2/M phase transition |
| + |
CDK2 | down-regulates
phosphorylation
|
CDC25A |
0.823 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-150839 |
Ser263 |
CKLFDSPsLCSSSTR |
Homo sapiens |
|
| pmid |
sentence |
| 17110335 |
We show here that dna-responsive checkpoints activate pp2a/b56delta phosphatase complexes to dephosphorylate cdc25 at a site distinct from ser287 (t138), the phosphorylation of which is required for 14-3-3 release. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RPS6K | down-regulates
phosphorylation
|
CDC25A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252793 |
Ser293 |
GSTKRRKsMSGASPK |
Homo sapiens |
|
| pmid |
sentence |
| 23708659 |
Rsk promotes g2/m transition through activating phosphorylation of cdc25a and cdc25b rsk is likely to be more active in mitotic cells than in interphase cells, as evidenced by the phosphorylation status of t359/s363 in rsk. Together, these findings indicate that rsk promotes g2/m transition in mammalian cells through activating phosphorylation of cdc25a and cdc25b. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252776 |
Ser295 |
TKRRKSMsGASPKES |
Homo sapiens |
|
| pmid |
sentence |
| 23708659 |
Rsk promotes g2/m transition through activating phosphorylation of cdc25a and cdc25b rsk is likely to be more active in mitotic cells than in interphase cells, as evidenced by the phosphorylation status of t359/s363 in rsk. Together, these findings indicate that rsk promotes g2/m transition in mammalian cells through activating phosphorylation of cdc25a and cdc25b. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
RPS6KA1 | down-regulates
phosphorylation
|
CDC25A |
0.373 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-202113 |
Ser293 |
GSTKRRKsMSGASPK |
Homo sapiens |
|
| pmid |
sentence |
| 23708659 |
Rsk promotes g2/m transition through activating phosphorylation of cdc25a and cdc25b rsk is likely to be more active in mitotic cells than in interphase cells, as evidenced by the phosphorylation status of t359/s363 in rsk. Together, these findings indicate that rsk promotes g2/m transition in mammalian cells through activating phosphorylation of cdc25a and cdc25b. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-202117 |
Ser295 |
TKRRKSMsGASPKES |
Homo sapiens |
|
| pmid |
sentence |
| 23708659 |
Rsk promotes g2/m transition through activating phosphorylation of cdc25a and cdc25b rsk is likely to be more active in mitotic cells than in interphase cells, as evidenced by the phosphorylation status of t359/s363 in rsk. Together, these findings indicate that rsk promotes g2/m transition in mammalian cells through activating phosphorylation of cdc25a and cdc25b. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
PKM |
0.499 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276967 |
Ser37 |
MCRLDIDsPPITARN |
Homo sapiens |
|
| pmid |
sentence |
| 27485204 |
Cdc25A dephosphorylates PKM2 at S37, and promotes PKM2 dependent beta-catenin transactivation and c-Myc-upregulated expression of the glycolytic genes GLUT1, PKM2 and LDHA, and of CDC25A; thus, Cdc25A upregulates itself in a positive feedback loop.|Cdc25A dephosphorylates PKM2 at S37, and promotes PKM2-dependent \u03b2-catenin transactivation and c-Myc-upregulated expression of the glycolytic genes GLUT1, PKM2 and LDHA, and of CDC25A; thus, Cdc25A upregulates itself in a positive feedback loop. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CyclinD3/CDK6 | down-regulates quantity by destabilization
phosphorylation
|
CDC25A |
0.614 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277343 |
Ser40 |
ASAAGGLsPVTNLTV |
Homo sapiens |
A-431 Cell |
| pmid |
sentence |
| 28192398 |
We demonstrate that CyclinD-CDK4/CDK6 complexes mediate the phosphorylation of CDC25A on Ser40 during G1 and that these complexes directly phosphorylate this residue in vitro. Importantly, we also find that CyclinD1-CDK4 decreases CDC25A stability in a ßTrCP-dependent manner and that Ser40 and Ser88 phosphorylations contribute to this regulation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277341 |
Ser88 |
DSGFCLDsPGPLDSK |
Homo sapiens |
A-431 Cell |
| pmid |
sentence |
| 28192398 |
We demonstrate that CyclinD-CDK4/CDK6 complexes mediate the phosphorylation of CDC25A on Ser40 during G1 and that these complexes directly phosphorylate this residue in vitro. Importantly, we also find that CyclinD1-CDK4 decreases CDC25A stability in a ßTrCP-dependent manner and that Ser40 and Ser88 phosphorylations contribute to this regulation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CyclinD/CDK4 | down-regulates quantity by destabilization
phosphorylation
|
CDC25A |
0.637 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277340 |
Ser40 |
ASAAGGLsPVTNLTV |
Homo sapiens |
A-431 Cell |
| pmid |
sentence |
| 28192398 |
We demonstrate that CyclinD-CDK4/CDK6 complexes mediate the phosphorylation of CDC25A on Ser40 during G1 and that these complexes directly phosphorylate this residue in vitro. Importantly, we also find that CyclinD1-CDK4 decreases CDC25A stability in a ßTrCP-dependent manner and that Ser40 and Ser88 phosphorylations contribute to this regulation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277342 |
Ser88 |
DSGFCLDsPGPLDSK |
Homo sapiens |
A-431 Cell |
| pmid |
sentence |
| 28192398 |
We demonstrate that CyclinD-CDK4/CDK6 complexes mediate the phosphorylation of CDC25A on Ser40 during G1 and that these complexes directly phosphorylate this residue in vitro. Importantly, we also find that CyclinD1-CDK4 decreases CDC25A stability in a ßTrCP-dependent manner and that Ser40 and Ser88 phosphorylations contribute to this regulation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
GSK3B | down-regulates quantity by repression
phosphorylation
|
CDC25A |
0.318 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-164742 |
Ser76 |
SNLQRMGsSESTDSG |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 20348946 |
Here, we report that casein kinase 1 alpha (ck1alpha) phosphorylates cdc25a on both s79 and s82 in a hierarchical manner requiring prior phosphorylation of s76 by chk1 or gsk-3beta. This facilitates beta-trcp binding and ubiquitin-mediated proteolysis of cdc25a |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CSNK1A1 | down-regulates
phosphorylation
|
CDC25A |
0.339 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-164734 |
Ser79 |
QRMGSSEsTDSGFCL |
Homo sapiens |
|
| pmid |
sentence |
| 20348946 |
Here, we report that casein kinase 1 alpha (ck1alpha) phosphorylates cdc25a on both s79 and s82 in a hierarchical manner requiring prior phosphorylation of s76 by chk1 or gsk-3beta. This facilitates beta-trcp binding and ubiquitin-mediated proteolysis of cdc25a |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-164738 |
Ser82 |
GSSESTDsGFCLDSP |
Homo sapiens |
|
| pmid |
sentence |
| 20348946 |
Here, we report that casein kinase 1 alpha (ck1alpha) phosphorylates cdc25a on both s79 and s82 in a hierarchical manner requiring prior phosphorylation of s76 by chk1 or gsk-3beta. This facilitates beta-trcp binding and ubiquitin-mediated proteolysis of cdc25a |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEK11 | down-regulates
phosphorylation
|
CDC25A |
0.427 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-187867 |
Ser82 |
GSSESTDsGFCLDSP |
Homo sapiens |
|
| pmid |
sentence |
| 19734889 |
Nek11 regulates cdc25a degradation and the ir-induced g2/m checkpointincubation of wild-type cdc25a with nek11 led to a marked increase in phosphorylation of ser 82 and 88 as detected with the phosphospecific antibody recognizing these sites |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-187871 |
Ser88 |
DSGFCLDsPGPLDSK |
Homo sapiens |
|
| pmid |
sentence |
| 19734889 |
Nek11 regulates cdc25a degradation and the ir-induced g2/m checkpointincubation of wild-type cdc25a with nek11 led to a marked increase in phosphorylation of ser 82 and 88 as detected with the phosphospecific antibody recognizing these sites |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CDK1 |
0.836 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248479 |
Thr14 |
IEKIGEGtYGVVYKG |
Homo sapiens |
|
| pmid |
sentence |
| 10454565 |
Phosphatase activity of Cdc25A is critical for its activating capacity (data not shown). In this context, it should also be mentioned that Cdc25A is able to activate cyclin B-Cdk1 in vitro |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248480 |
Tyr15 |
EKIGEGTyGVVYKGR |
Homo sapiens |
|
| pmid |
sentence |
| 10454565 |
Phosphatase activity of Cdc25A is critical for its activating capacity (data not shown). In this context, it should also be mentioned that Cdc25A is able to activate cyclin B-Cdk1 in vitro |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CDK2 |
0.823 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248481 |
Thr14 |
VEKIGEGtYGVVYKA |
Homo sapiens |
|
| pmid |
sentence |
| 10454565 |
The phosphatase activity of Cdc25A is necessary for Cdk2 activation, most likely due to dephosphorylation on Tyr-15 and Thr-14 of Cdk2. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248482 |
Tyr15 |
EKIGEGTyGVVYKAR |
Homo sapiens |
|
| pmid |
sentence |
| 10454565 |
The phosphatase activity of Cdc25A is necessary for Cdk2 activation, most likely due to dephosphorylation on Tyr-15 and Thr-14 of Cdk2. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245449 |
|
|
Homo sapiens |
MCF-7 Cell |
| pmid |
sentence |
| 11154267 |
Expression of Cdc25A mRNA and protein was induced by E(2) in control and p16(INK4a)-expressing MCF-7 cells; however, functional activity of Cdc25A was inhibited in cells expressing p16(INK4a). Inhibition of Cdc25A activity in p16(INK4a)-expressing cells was associated with depressed Cdk2 activity |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates
dephosphorylation
|
CDK2 |
0.823 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-95252 |
Thr14 |
VEKIGEGtYGVVYKA |
Homo sapiens |
|
| pmid |
sentence |
| 12411508 |
Cell division cycle 25 a (cdc25a), a dual-specificity protein phosphatase, is one of the most crucial cell cycle regulators, which removes the inhibitory phosphorylation in cyclin-dependent kinases (cdks), such as cdk2, cdk4, and cdk6, and positively regulates the activities of cdks that lead to cell cycle progression. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195521 |
Thr14 |
VEKIGEGtYGVVYKA |
Homo sapiens |
|
| pmid |
sentence |
| 22263797 |
Cell division cycle 25 a (cdc25a), a dual-specificity protein phosphatase, is one of the most crucial cell cycle regulators, which removes the inhibitory phosphorylation in cyclin-dependent kinases (cdks), such as cdk2, cdk4, and cdk6, and positively regulates the activities of cdks that lead to cell cycle progression. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
PLK3 | down-regulates
phosphorylation
|
CDC25A |
0.391 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-160228 |
Thr80 |
RMGSSEStDSGFCLD |
Homo sapiens |
|
| pmid |
sentence |
| 18167338 |
Here, we demonstrate that glycogen synthase kinase-3beta (gsk-3beta) phosphorylates cdc25a to promote its proteolysis in early cell-cycle phases. Phosphorylation by gsk-3beta requires priming of cdc25a, and this can be catalyzed by polo-like kinase 3 (plk-3) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CDK4 |
0.693 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267568 |
Tyr17 |
AEIGVGAyGTVYKAR |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23429262 |
Invalidation of CDK4 has no impact by itself on the cell proliferation, but invalidation of CDC25A prevents the dephosphorylation of CDK6 (Y24) and CDK4 (Y17) residues, and impedes their association with CCNDs. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277138 |
Tyr17 |
AEIGVGAyGTVYKAR |
Homo sapiens |
|
| pmid |
sentence |
| 27485204 |
Cdc25A mainly promotes G1-S transition by dephosphorylating CDK4 (Y17)-cyclin D, CDK6 (Y24)-cyclin D and CDK2 (T14 and Y15)-cyclin E/A complexes and G2-M progression by dephosphorylating CDK1 (T14 and Y15)-cyclin A/B .|Cdc25A mainly promotes G1-S transition by dephosphorylating CDK4 (Y17)-cyclin D, CDK6 (Y24)-cyclin D and CDK2 (T14/Y15)-cyclin E/A complexes and G2-M progression by dephosphorylating CDK1 (T14/Y15)-cyclin A/B . |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CDK6 |
0.692 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267569 |
Tyr24 |
AEIGEGAyGKVFKAR |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23429262 |
Invalidation of CDK4 has no impact by itself on the cell proliferation, but invalidation of CDC25A prevents the dephosphorylation of CDK6 (Y24) and CDK4 (Y17) residues, and impedes their association with CCNDs. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
CDC25A |
0.492 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277344 |
|
|
Homo sapiens |
A-431 Cell |
| pmid |
sentence |
| 28192398 |
We demonstrate that CyclinD-CDK4/CDK6 complexes mediate the phosphorylation of CDC25A on Ser40 during G1 and that these complexes directly phosphorylate this residue in vitro. Importantly, we also find that CyclinD1-CDK4 decreases CDC25A stability in a ßTrCP-dependent manner and that Ser40 and Ser88 phosphorylations contribute to this regulation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMAD3 | down-regulates quantity by repression 
transcriptional regulation
|
CDC25A |
0.55 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245445 |
|
|
Homo sapiens |
T-lymphocyte |
| pmid |
sentence |
| 22740686 |
PD-1 also inhibited phosphorylation of the transcription factor Smad3, which increased its activity. These events induced additional inhibitory checkpoints in the cell cycle by increasing the abundance of the G(1) phase inhibitor p15(INK4) and repressing the Cdk-activating phosphatase Cdc25A |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
BTRC | up-regulates
ubiquitination
|
CDC25A |
0.507 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-128436 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15340381 |
Scfb-trcp has recently been shown to degrade phosphorylated cdc25a in the s and g2 phases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | down-regulates activity
dephosphorylation
|
DYRK2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277098 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 34363019 |
Finally, DYRK2 is dephosphorylated by CDC25A, suggesting a feedback regulatory loop.|Notably, the co-expression of CDC25A inhibited the DYRK2 pro-apoptotic effect (Fig.\u00a06D), concurring with a possible inhibitory role on DYRK2 activity and further suggesting that the effect of DYRK2 was dependent on CDC25A. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CCNB1 |
0.849 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277137 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16682204 |
Cdc25A dephosphorylates and activates CyclinE\u2013Cdk2, CyclinA\u2013Cdk2 and CyclinB\u2013Cdk1, whereas Cdc25B and Cdc25C primarily target CyclinB\u2013Cdk1 [4,5] . |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
ubiquitination
|
CDC25A |
0.476 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271388 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12234927 |
We found that Cdc25 A degradation during mitotic exit and in early G(1) is mediated by the anaphase-promoting complex or cyclosome (APC/C)(Cdh1) ligase, and that a KEN-box motif in the N-terminus of the protein is required for its targeted degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
FOXO1 |
0.318 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277139 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21670150 |
In this study, we revealed that Cdc25A enhances Foxo1 stability by dephosphorylating Cdk2, and Foxo1 was shown to directly regulate transcription of the metastatic factor MMP1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
E2F1 | up-regulates quantity by expression 
transcriptional regulation
|
CDC25A |
0.535 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245468 |
|
|
Homo sapiens |
MCF-7 Cell |
| pmid |
sentence |
| 11154267 |
Expression of Cdc25A is transcriptionally regulated by Myc and E2F-1 , both of which are expressed in MCF-7 cells in response to estrogen |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
MYC | up-regulates quantity by expression
transcriptional regulation
|
CDC25A |
0.611 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245465 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11154267 |
Expression of Cdc25A is transcriptionally regulated by Myc and E2F-1 , both of which are expressed in MCF-7 cells in response to estrogen |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
CDC25A | down-regulates
dephosphorylation
|
MAPK3 |
0.404 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-133392 |
|
|
Homo sapiens |
MCF-7 Cell |
| pmid |
sentence |
| 15672448 |
We found that cdc25a physically interacted with and de-phosphorylated phospho-erk both in vitro and in cell culture. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates
phosphorylation
|
CDC25A |
0.372 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-152996 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17292828 |
Mk2 was required for the degradation of cdc25a. Mk2 phosphorylates cdc25a in vitro. Phosphorylation of cdc25a in vivo has been shown to facilitate its ubiquitin-mediated proteolysis |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC14A | down-regulates activity
dephosphorylation
|
CDC25A |
0.516 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277065 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20956543 |
Cdc14A inhibits Cdc25A and Cdc25B activity, the latter through direct binding and dephosphorylation ( ).|Indeed, in vitro dephosphorylation of Cdk1-cyclin B1-phosphorylated Cdc25A by Cdc14A did not inhibit its catalytic activity (data not shown).|Taken together, our results suggest that at the G2/M transition Cdc14A acts on an unknown protein, which in turn inhibits Cdc25A phosphatase activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | down-regulates
dephosphorylation
|
RAF1 |
0.403 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-32548 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 7744247 |
Cdc25a can act on substrates other than cdks, since it dephosphorylates the homeodomain transcription factor cut and interacts with and dephosphorylates the proto-oncogene raf-1, resulting in a significant decrease in raf-1 kinase activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CyclinE/CDK2 |
0.736 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245452 |
|
|
Homo sapiens |
MCF-7 Cell |
| pmid |
sentence |
| 11154267 |
Cyclin E-Cdk2 complexes from p16INK4a-expressing MCF-7 cells are activated in vitro and in vivo by Cdc25A |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
SCF-betaTRCP | up-regulates
ubiquitination
|
CDC25A |
0.492 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217178 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15340381 |
Scfb-trcp has recently been shown to degrade phosphorylated cdc25a in the s and g2 phases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CCNA2 |
0.678 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277136 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16682204 |
Cdc25A dephosphorylates and activates CyclinE\u2013Cdk2, CyclinA\u2013Cdk2 and CyclinB\u2013Cdk1, whereas Cdc25B and Cdc25C primarily target CyclinB\u2013Cdk1 [4,5] . |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC25A | up-regulates activity
dephosphorylation
|
CyclinD/CDK4 |
0.637 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245456 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23429262 |
We show that the miRNA-induced silencing of CDC25A increases the tyrosine phosphorylation status of CDK4/6 cyclin-dependent kinases which, in turn, abolishes CDK4/6 capacity to associate with D-type cyclins. This prevents CDK4/6 kinases activation, impairs downstream events such as cyclin E stimulation and sequesters cells in early G1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
3.0
CDC25A
- 
- 