| + |
SCF-betaTRCP | down-regulates quantity by destabilization 
polyubiquitination
|
LPCAT1 |
0.333 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272759 |
Lys221 |
RTCLITFkPGAFIPG |
Mus musculus |
Lung Epithelial Cell |
| pmid |
sentence |
| 21068446 |
Here, we show that lipopolysaccharide (LPS) causes LPCAT1 degradation using the Skp1-Cullin-F-box ubiquitin E3 ligase component, β-transducin repeat-containing protein (β-TrCP), that polyubiquitinates LPCAT1, thereby targeting the enzyme for proteasomal degradation.Lys221 Is a Bona Fide Site for LPCAT1 Ubiquitination |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
FOXP3 |
0.262 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277246 |
Lys356 |
AILEAPEkQRTLNEI |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 27432879 |
The ubiquitination site of Foxp3 is Lys356 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
NHEJ1 |
0.329 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276882 |
Thr181 |
LIRDRLKtEPFEENS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 25661488 |
Here we report that Akt phosphorylates XLF (XRCC4 like factor, also called NHEJ1) at T181, to dissociate XLF from the XRCC4 (X-ray repair cross-complementing protein 4)/DNA ligase IV (LIG4) complex and subsequently triggers XLF cytoplasmic translocation, leading to XLF ubiquitination by SCFβ-TRCP in a CKI-dependent manner. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
TOP2B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277511 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32015321 |
Specifically, DNA damage signal, triggered by teniposide (VM-26) treatment, activates ATM, cooperating with CK1 to phosphorylate TOP2β on Ser1134 and Ser1130, respectively, in a canonical degron motif to facilitate β-TrCP binding and subsequent degradation.ATM binds with and phosphorylates TOP2β at Ser1134 to promote its degradation by VM-26. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
TIAM1 |
0.345 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276675 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 25124033 |
Phosphorylation of Ser329, Ser334, and Thr340 in Tiam1 is required for its interaction with βTrCP1. The proteolysis of Tiam1 is prevented by βTrCP silencing, inhibition of CK1 and MEK, or mutation of the Tiam1 degron site. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
WEE1 |
0.396 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276633 |
|
|
in vitro |
|
| pmid |
sentence |
| 24817118 |
Casein kinase 1-mediated N-terminal Weee1 phosphorylation is required for interaction with the F-box protein β-TrCP.MS/MS spectra of human Wee1 identifying serine 212 as phosphorylated after incubation with recombinant CK1δ. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
COMMD1 |
0.444 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271430 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 20068069 |
CLU-2 is a ubiquitin binding protein (UBP) that enhances proteasome activity. sCLU promotes degradation of COMMD1. sCLU interacts with the SCF-βTrCP E3 ligase complex, serving as a scaffolding chaperone to form a multimeric protein complex that facilitates COMMD1 and I-κB ubiquitination and proteasomal degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
SMAD3 |
0.433 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272944 |
|
|
Chlorocebus aethiops |
COS-7 Cell |
| pmid |
sentence |
| 11359933 |
Smad3 interacts with a RING finger protein, ROC1, through its C-terminal MH2 domain in a ligand-dependent manner. An E3 ubiquitin ligase complex ROC1-SCF(Fbw1a) consisting of ROC1, Skp1, Cullin1, and Fbw1a (also termed betaTrCP1) induces ubiquitination of Smad3. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
SCF-betaTRCP | up-regulates quantity 
ubiquitination
|
MYC |
0.482 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-243542 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20852628 |
Here we show that SCF²-TrCP binds to Myc by means of a characteristic phosphodegron and ubiquitylates Myc; this results in enhanced Myc stability. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
CELF6 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269043 |
|
|
Homo sapiens |
HCT-116 Cell |
| pmid |
sentence |
| 31534127 |
CELF6 is degraded by ubiquitin-dependent proteasome pathway. The cell cycle-dependent expression of CELF6 is mediated through the ubiquitin-proteasome pathway, SCF-β-TrCP recognizes a nonphospho motif in CELF6 and regulates its proteasomal degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
BHLHE40 |
0.351 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276853 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 25202122 |
During unperturbed cell cycles, DEC1 is a highly unstable protein that is targeted for proteasome-dependent degradation by the SCF(βTrCP) ubiquitin ligase in cooperation with CK1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CUL1 | form complex 
binding
|
SCF-betaTRCP |
0.884 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-64502 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10023660 |
The human f box protein beta-trcp associates with the cul1/skp1 complex and regulates the stability of beta-catenin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | up-regulates
ubiquitination
|
CDC25A |
0.493 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217178 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15340381 |
Scfb-trcp has recently been shown to degrade phosphorylated cdc25a in the s and g2 phases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
CD274 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277277 |
|
|
Homo sapiens |
BT-549 Cell |
| pmid |
sentence |
| 27572267 |
We show that glycogen synthase kinase 3β (GSK3β) interacts with PD-L1 and induces phosphorylation-dependent proteasome degradation of PD-L1 by β-TrCP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CLU | up-regulates activity
binding
|
SCF-betaTRCP |
0.318 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271429 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 20068069 |
CLU-2 is a ubiquitin binding protein (UBP) that enhances proteasome activity. sCLU promotes degradation of COMMD1. sCLU interacts with the SCF-βTrCP E3 ligase complex, serving as a scaffolding chaperone to form a multimeric protein complex that facilitates COMMD1 and I-κB ubiquitination and proteasomal degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
YBX1 |
0.327 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271606 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16797541 |
Here we identify FBX33 as a component of an SCF E3-ubiquitin ligase that targets the multifunctional regulator Y-box binding protein 1 (YB-1)/dbpB/p50 for polyubiquitination and destruction by the proteasome. By targeting YB-1 for proteasomal degradation, FBX33 negatively interferes with YB-1 mediated functions. FBX33 recruits Skp-1/Cul1 to YB-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
ATF4 |
0.334 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272580 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 11238952 |
Here we show that the F-box protein betaTrCP, the receptor component of the SCF E3 ubiquitin ligase responsible for IkappaBalpha and beta-catenin degradation, is colocalized in the nucleus with ATF4, a member of the ATF-CREB bZIP family of transcription factors, and controls its stability. ATF4 ubiquitination in HeLa cells is enhanced in the presence of betaTrCP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates
ubiquitination
|
CTNNB1 |
0.717 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217181 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10023660 |
These results indicate that the cul1/skp1/beta-trcp complex forms a ubiquitin ligase that mediates the degradation of beta-catenin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BTRC | form complex
binding
|
SCF-betaTRCP |
0.818 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-64496 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10023660 |
The human f box protein beta-trcp associates with the cul1/skp1 complex and regulates the stability of beta-catenin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SKP1 | form complex
binding
|
SCF-betaTRCP |
0.892 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-64514 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10023660 |
The human f box protein beta-trcp associates with the cul1/skp1 complex and regulates the stability of beta-catenin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates
ubiquitination
|
CDKN1B |
0.522 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217172 |
|
|
Homo sapiens |
Leukemia Cell |
| pmid |
sentence |
| 12835716 |
Furthermore, c-myc activation can also promote the degradation of p27kip1 protein by directly activating the cul1 gene, which encodes a critical component of the ubiquitin ligase scfskp2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
IKBKB |
0.559 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271431 |
|
|
Homo sapiens |
LNCaP Cell |
| pmid |
sentence |
| 20068069 |
CLU-2 is a ubiquitin binding protein (UBP) that enhances proteasome activity. sCLU promotes degradation of COMMD1. sCLU interacts with the SCF-βTrCP E3 ligase complex, serving as a scaffolding chaperone to form a multimeric protein complex that facilitates COMMD1 and I-κB ubiquitination and proteasomal degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
YAP1 |
0.372 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217187 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
This cascade of phosphorylation allows the binding of scfbetatrcp that promotes the ubiquitination and degradation of yap. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
FBXO33 | up-regulates activity
binding
|
SCF-betaTRCP |
0.475 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271605 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16797541 |
Here we identify FBX33 as a component of an SCF E3-ubiquitin ligase that targets the multifunctional regulator Y-box binding protein 1 (YB-1)/dbpB/p50 for polyubiquitination and destruction by the proteasome. By targeting YB-1 for proteasomal degradation, FBX33 negatively interferes with YB-1 mediated functions. FBX33 recruits Skp-1/Cul1 to YB-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
PER2 |
0.466 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268055 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17876059 |
Here, the authors show that the F-box protein beta-transducin repeat containing protein 1 (beta-TrCP1) as part of the E3 ubiquitin ligase complex is an essential component of the mammalian circadian oscillator. Down-regulation of endogenous beta-TrCP1 as well as expression of a dominant-negative form both result in lengthening of the circadian period in oscillating fibroblasts. These phenotypes are due to an impaired degradation of PERIOD (PER) proteins, since expression of beta-TrCP interaction-deficient PER2 variants--but not wild-type PER2--results in a dramatic stabilization of PER2 protein as well as in the disruption of circadian rhythmicity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Circadian clock |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
CD4 |
0.255 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272615 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11893391 |
Degradation of the HIV receptor CD4 by the proteasome, mediated by the HIV-1 protein Vpu, is crucial for the release of fully infectious virions. To promote CD4 degradation Vpu has to be phosphorylated on a motif DSGXXS, which is conserved in several signalling proteins known to be degraded by the proteasome upon phosphorylation. Such phosphorylation is required for the interaction of Vpu with the ubiquitin ligase SCF-beta-TrCP that triggers CD4 degradation by the proteasome. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
CDC25A |
0.493 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277344 |
|
|
Homo sapiens |
A-431 Cell |
| pmid |
sentence |
| 28192398 |
We demonstrate that CyclinD-CDK4/CDK6 complexes mediate the phosphorylation of CDC25A on Ser40 during G1 and that these complexes directly phosphorylate this residue in vitro. Importantly, we also find that CyclinD1-CDK4 decreases CDC25A stability in a ßTrCP-dependent manner and that Ser40 and Ser88 phosphorylations contribute to this regulation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
YAP/TAZ |
0.391 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277637 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
Phosphorylation of YAP (S381) and TAZ (S311) by Lats1/2 primes subsequent phosphorylation events by casein kinase 1 (CK1d/e); this sequential phosphorylation results in recruitment of b-transducin repeat-containing proteins (b-TRCP; a subunit of the SCF ubiquitin E3 ligase) and consequently leads to degradation of YAP/TAZ |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates
binding
|
WEE1 |
0.396 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217184 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15340381 |
Scfb-trcp continues to have a role in this phase, however, through its induced degradation of the cdk1 inhibitor, wee1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
RAPGEF2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276607 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 24290981 |
Here, we report that in response to factors that promote cell motility, the Rap guanine exchange factor RAPGEF2 is rapidly phosphorylated by I-kappa-B-kinase-β and casein kinase-1α and consequently degraded by the proteasome via the SCF(βTrCP) ubiquitin ligase. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276606 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 24290981 |
Here, we report that in response to factors that promote cell motility, the Rap guanine exchange factor RAPGEF2 is rapidly phosphorylated by I-kappa-B-kinase-β and casein kinase-1α and consequently degraded by the proteasome via the SCF(βTrCP) ubiquitin ligase. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
MTSS1 |
0.324 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276610 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 24318128 |
Mechanistically, we defined that Casein Kinase Iδ (CKIδ) phosphorylates Ser322 to trigger MTSS1's interaction with β-TRCP for subsequent ubiquitination and degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
GBF1 |
0.253 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277399 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 29898406 |
We show that, in mitosis, GBF1 is phosphorylated on Ser292 and Ser297 by casein kinase-2 allowing recognition by the F-box protein βTrCP. GBF1 interaction with βTrCP recruits GBF1 to the SCFβTrCP ubiquitin ligase complex, triggering its degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
CEP68 |
0.343 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275622 |
|
|
|
|
| pmid |
sentence |
| 25503564 |
We show that the intercentrosomal linker protein Cep68 is degraded in prometaphase through the SCF(βTrCP) (Skp1-Cul1-F-box protein) ubiquitin ligase complex. Cep68 degradation is initiated by PLK1 phosphorylation of Cep68 on Ser 332, allowing recognition by βTrCP. |
|
| Publications: |
1 |
| + |
SCF-betaTRCP | up-regulates activity
ubiquitination
|
NFKB2 |
0.534 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217175 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 14676825 |
Mechanism of processing of the nf-kappa b2 p100 precursor: identification of the specific polyubiquitin chain-anchoring lysine residue and analysis of the role of nedd8-modification on the scf(beta-trcp) ubiquitin ligase. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | NF-KB Non Canonical |
| + |
SCF-betaTRCP | up-regulates activity
ubiquitination
|
NfKb-p65/p50 |
0.491 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-235305 |
|
|
Chlorocebus aethiops |
|
| pmid |
sentence |
| 11295495 |
The scf-betatrcp complex is responsible for the ubiquitination of p100 and p105 following their phosphorylation by ikk. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| Pathways: | NF-KB Canonical |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
CDH1 |
0.293 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274055 |
|
|
|
|
| pmid |
sentence |
| 23972993 |
Priming phosphorylation of Cdh1 by the Cdk2/cyclin A kinase complex allows Plk1 to bind to Cdh1 and phosphorylate Cdh1 at Ser138 and Ser146. Phosphorylation of Cdh1 at Ser138 and Ser146 then triggers its interaction with, and subsequent ubiquitination by, SCFbeta-TRCP |
|
| Publications: |
1 |
| Pathways: | Hippo Signaling |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
WWTR1 |
0.419 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-230750 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23431053 |
Phosphorylation of YAP (S381) and TAZ (S311) by Lats1/2 primes subsequent phosphorylation events by casein kinase 1 (CK1d/e); this sequential phosphorylation results in recruitment of b-transducin repeat-containing proteins (b-TRCP; a subunit of the SCF ubiquitin E3 ligase) and consequently leads to degradation of YAP/TAZ |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hippo Signaling |
| + |
SCF-betaTRCP | up-regulates activity
ubiquitination
|
NFKB1 |
0.501 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217190 |
|
|
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 11295495 |
The scf-betatrcp complex is responsible for the ubiquitination of p100 and p105 following their phosphorylation by ikk. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
CDC34 | up-regulates activity
binding
|
SCF-betaTRCP |
0.766 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277331 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25425648 |
The ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase SCF are capable of building polyubiquitin chains onto protein substrates both rapidly and processively; this may be explained at least in part by the atypically fast rate of Cdc34 and SCF association.Here, we use protein cross-linking to demonstrate that the Cdc34-SCF interaction occurs in multiple conformations, where several residues from the Cdc34 acidic tail are capable of contacting a broad region of the SCF basic canyon. Similar patterns of cross-linking are also observed between Cdc34 and the Cul1 paralog Cul2, implicating the same mechanism for the Cdc34-SCF interaction in other members of the cullin-RING ubiquitin ligases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
polyubiquitination
|
PSMD4 |
0.523 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272751 |
|
|
in vitro |
|
| pmid |
sentence |
| 19240029 |
S5a/Rpn10 is a ubiquitin (Ub)-binding protein that is a subunit of the 26S proteasome but also exists free in the cytosol. It binds poly-Ub chains through its two Ub-interacting motifs (UIMs). We discovered that, unlike typical substrates of Ub ligases (E3s), S5a can be ubiquitinated by all E3s tested including multimeric and monomeric Ring finger E3s (MuRF1, Siah2, Parkin, APC, and SCF(betaTRCP1)), the U-box E3, CHIP, and HECT domain E3s (E6AP and Nedd4) when assayed with UbcH5 or related Ub-conjugating enzymes.The short half-life of S5a presumably is because of the presence of the UIM domain and reflects the ubiquitination of free S5a by many E3s. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SCF-betaTRCP | down-regulates quantity by destabilization
ubiquitination
|
CDC25B |
0.386 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276353 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 21807946 |
Recently, we showed that Cdc25B is degraded rapidly by non-genotoxic stimuli that activate stress-responsive MAPKs, such as Jun N-terminal kinase (JNK) and p38 (Uchida et al., 2009). Our results suggested that these kinases phosphorylate specific Ser residues in the N-terminal region (S101 and S103) to induce Cdc25B degradation.Here, we report that Cdc25B was ubiquitylated by SCF(βTrCP) E3 ligase upon phosphorylation at two Ser residues in the βTrCP-binding-motif-like sequence D(94)AGLCMDSPSP(104). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
SCF-betaTRCP