| + |
MAPKAPK2 |
phosphorylation
|
SRF |
0.582 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-67448 |
Ser103 |
RGLKRSLsEMEIGMV |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 10318869 |
Mk2 phosphorylates srf in vitro at ser-103 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 |
phosphorylation
|
RTN4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-139486 |
Ser107 |
VAPERQPsWDPSPVS |
Homo sapiens |
HeLa Cell, Macrophage |
| pmid |
sentence |
| 16095439 |
Nogo-b is phosphorylated at ser107 in vitro by mapkap-k2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity 
phosphorylation
|
POU5F1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279542 |
Ser111 |
ESNSDGAsPEPCTVT |
Homo sapiens |
|
| pmid |
sentence |
| 32409685 |
MK2 mediated OCT4 transcriptional activation is a novel mechanism for activating the MYC oncogene in progressive disease neuroblastoma that provides a therapeutic target.|OCT4 phosphorylation at the S111 residue by MK2 was upstream of MYC transcriptional activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates activity 
phosphorylation
|
ZFP36 |
0.697 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250156 |
Ser113 |
TELCRTFsESGRCRY |
in vitro |
|
| pmid |
sentence |
| 14688255 |
We confirm phosphorylation of TTP by MK2 and identify specific phosphorylation sites at Ser52, Ser105, Ser58, Ser176, Ser178, and Ser316. If MK2 regulates translation in part by TTP phosphorylation, TTP should be a repressor of translation when dephosphorylated and an activator of (or neutral to) translation when phosphorylated. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250153 |
Ser184 |
HPPVLRQsISFSGLP |
in vitro |
|
| pmid |
sentence |
| 14688255 |
We confirm phosphorylation of TTP by MK2 and identify specific phosphorylation sites at Ser52, Ser105, Ser58, Ser176, Ser178, and Ser316. If MK2 regulates translation in part by TTP phosphorylation, TTP should be a repressor of translation when dephosphorylated and an activator of (or neutral to) translation when phosphorylated. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250152 |
Ser186 |
PVLRQSIsFSGLPSG |
in vitro |
|
| pmid |
sentence |
| 14688255 |
We confirm phosphorylation of TTP by MK2 and identify specific phosphorylation sites at Ser52, Ser105, Ser58, Ser176, Ser178, and Ser316. If MK2 regulates translation in part by TTP phosphorylation, TTP should be a repressor of translation when dephosphorylated and an activator of (or neutral to) translation when phosphorylated. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250155 |
Ser60 |
RLPGRSTsLVEGRSC |
in vitro |
|
| pmid |
sentence |
| 14688255 |
We confirm phosphorylation of TTP by MK2 and identify specific phosphorylation sites at Ser52, Ser105, Ser58, Ser176, Ser178, and Ser316. If MK2 regulates translation in part by TTP phosphorylation, TTP should be a repressor of translation when dephosphorylated and an activator of (or neutral to) translation when phosphorylated. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250154 |
Ser66 |
TSLVEGRsCGWVPPP |
in vitro |
|
| pmid |
sentence |
| 14688255 |
We confirm phosphorylation of TTP by MK2 and identify specific phosphorylation sites at Ser52, Ser105, Ser58, Ser176, Ser178, and Ser316. If MK2 regulates translation in part by TTP phosphorylation, TTP should be a repressor of translation when dephosphorylated and an activator of (or neutral to) translation when phosphorylated. |
|
| Publications: |
5 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
CREB1 |
0.689 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-44384 |
Ser119 |
EILSRRPsYRKILND |
Homo sapiens |
|
| pmid |
sentence |
| 8887554 |
We show that mapkap kinase-2 phosphorylates creb at ser133 in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166619 |
Ser119 |
EILSRRPsYRKILND |
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Neverthless, some transcription factors, such as e47, er81, srf and creb are also phosphorylated by mk2. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | FLT3-ITD signaling, P38 Signaling |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
CREB1 |
0.689 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-153944 |
Ser119 |
EILSRRPsYRKILND |
Homo sapiens |
|
| pmid |
sentence |
| 17389598 |
Neverthless, some transcription factors, such as e47, er81, srf and creb are also phosphorylated by mk2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | FLT3-ITD signaling, P38 Signaling |
| + |
MAPKAPK2 | down-regulates activity
phosphorylation
|
HSF1 |
0.517 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279541 |
Ser121 |
NIKRKVTsVSTLKSE |
Homo sapiens |
|
| pmid |
sentence |
| 16278218 |
A potential mechanism for MK2-induced HSF1 inactivation is suggested by the findings that phosphorylation of serine 121 enhances HSF1 binding to HSP90, a major repressor of HSF1.|Phosphorylation of HSF1 by MAPK activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 |
phosphorylation
|
TSC2 |
0.657 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-98201 |
Ser1254 |
TALYKSLsVPAASTA |
Homo sapiens |
|
| pmid |
sentence |
| 12582162 |
Both in vitro and in vivo experiments demonstrate that the p38-activated kinase mk2 (also known as mapkapk2) is directly responsible for the phosphorylation of ser(1210). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
KRT20 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263071 |
Ser13 |
RSFHRSLsSSLQAPV |
in vitro |
|
| pmid |
sentence |
| 20724476 |
P38 phosphorylates the type II keratin, K8 at Ser73, whereas MK2 phosphorylates the binding partners K18 at Ser52 and K20 at Ser13. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263072 |
Ser13 |
RSFHRSLsSSLQAPV |
in vitro |
|
| pmid |
sentence |
| 20724476 |
P38 phosphorylates the type II keratin, K8 at Ser73, whereas MK2 phosphorylates the binding partners K18 at Ser52 and K20 at Ser13. |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | down-regulates
phosphorylation
|
HSPB1 |
0.808 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166629 |
Ser15 |
FSLLRGPsWDPFRDW |
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Notably mk2 is well known to play an important role in actin filament remodellng by phosphorylating hsp27. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166633 |
Ser78 |
PAYSRALsRQLSSGV |
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Notably mk2 is well known to play an important role in actin filament remodellng by phosphorylating hsp27. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-94021 |
Ser78 |
PAYSRALsRQLSSGV |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 12367505 |
Notably mk2 is well known to play an important role in actin filament remodellng by phosphorylating hsp27. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-94025 |
Ser82 |
RALSRQLsSGVSEIR |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 12367505 |
Notably mk2 is well known to play an important role in actin filament remodellng by phosphorylating hsp27. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166637 |
Ser82 |
RALSRQLsSGVSEIR |
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Notably mk2 is well known to play an important role in actin filament remodellng by phosphorylating hsp27. |
|
| Publications: |
5 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates activity
phosphorylation
|
PDE4A |
0.347 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263078 |
Ser152 |
SFLYRSDsDYDMSPK |
Homo sapiens |
Macrophage |
| pmid |
sentence |
| 21323643 |
Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) by MK2 (MAPKAPK2) attenuates its activation through protein kinase A phosphorylation. In the present study, we show that PDE4A5 is phosphorylated at Ser147, within the regulatory UCR1 (ultraconserved region 1) domain conserved among PDE4 long isoforms, by MK2 (MAPK-activated protein kinase 2, also called MAPKAPK2). Phosphorylation by MK2, although not altering PDE4A5 activity, markedly attenuates PDE4A5 activation through phosphorylation by protein kinase A. This modification confers the amplification of intracellular cAMP accumulation in response to adenylate cyclase activation by attenuating a major desensitization system to cAMP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates quantity by stabilization
phosphorylation
|
MDM2 |
0.365 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-133560 |
Ser166 |
SSRRRAIsETEENSD |
Homo sapiens |
|
| pmid |
sentence |
| 15688025 |
Hdm2 phosphorylation by mapkap kinase 2 enhances hdm2 activity and promote the degradation of p53. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | FLT3-ITD signaling |
| + |
MAPKAPK2 | down-regulates activity
phosphorylation
|
RCSD1 |
0.478 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263079 |
Ser179 |
RRFRRSQsDCGELGD |
in vitro |
|
| pmid |
sentence |
| 15850461 |
Human CapZIP was phosphorylated at Ser-179 and Ser-244 by MAPKAP-K2 (mitogen-activated protein kinase-activated protein kinase 2) or MAPKAP-K3 in vitro. In the present paper we have identified CapZIP as a protein that is phosphorylated exceptionally rapidly by several SAPKs in vitro (Figure 4), and which is expressed in muscles and immune cells. Both MAPKAP-K2 and MAPKAP-K3 phosphorylated CapZIP at Ser-179 in vitro. An important clue to the function of CapZIP and its phosphorylation came from the finding that it binds to the actin-capping protein CapZ (Figure 7A), and that cellular stresses trigger the dissociation of these two proteins (Figure 7B).Such an effect is presumably lost when CapZIP is phosphorylated and dissociates from CapZ. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263080 |
Ser244 |
PPLRRSPsRTEKQEE |
in vitro |
|
| pmid |
sentence |
| 15850461 |
Human CapZIP was phosphorylated at Ser-179 and Ser-244 by MAPKAP-K2 (mitogen-activated protein kinase-activated protein kinase 2) or MAPKAP-K3 in vitro. In the present paper we have identified CapZIP as a protein that is phosphorylated exceptionally rapidly by several SAPKs in vitro (Figure 4), and which is expressed in muscles and immune cells. Both MAPKAP-K2 and MAPKAP-K3 phosphorylated CapZIP at Ser-179 in vitro. An important clue to the function of CapZIP and its phosphorylation came from the finding that it binds to the actin-capping protein CapZ (Figure 7A), and that cellular stresses trigger the dissociation of these two proteins (Figure 7B).Such an effect is presumably lost when CapZIP is phosphorylated and dissociates from CapZ. |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | down-regulates quantity by destabilization
phosphorylation
|
UBE2J1 |
0.335 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263091 |
Ser184 |
KELARQIsFKAEVNS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 24020373 |
Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNFα production. These findings strongly suggest that MK2 directly phosphorylates Ube2j1 at Ser(184) upon p38-activating stress in vivo. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
TH |
0.518 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250149 |
Ser19 |
KGFRRAVsELDAKQA |
in vitro |
|
| pmid |
sentence |
| 11359875 |
MAPKAP-K2 phosphorylates both Ser19 and Ser40 of TH. Tyrosine hydroxylase (TH) has been reported to require binding of 14-3-3 proteins for optimal activation by phosphorylation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250150 |
Ser40 |
GQGAPGPsLTGSPWP |
in vitro |
|
| pmid |
sentence |
| 11359875 |
MAPKAP-K2 phosphorylates both Ser19 and Ser40 of TH. Tyrosine hydroxylase (TH) has been reported to require binding of 14-3-3 proteins for optimal activation by phosphorylation. |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | down-regulates activity
phosphorylation
|
ETV1 |
0.61 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250145 |
Ser191 |
HRFRRQLsEPCNSFP |
Neovison vison |
|
| pmid |
sentence |
| 11551945 |
MK2 phosphorylates ER81 in vitro within its central inhibitory domain, and overexpression of MK2 leads to increased in vivo phosphorylation of ER81. Two serine residues, ER81 amino acids 191 and 216, were identified as MK2 phosphorylation sites. MK2 suppresses basal ER81-dependent transcription |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250146 |
Ser216 |
PMYQRQMsEPNIPFP |
Neovison vison |
|
| pmid |
sentence |
| 11551945 |
MK2 phosphorylates ER81 in vitro within its central inhibitory domain, and overexpression of MK2 leads to increased in vivo phosphorylation of ER81. Two serine residues, ER81 amino acids 191 and 216, were identified as MK2 phosphorylation sites. MK2 suppresses basal ER81-dependent transcription |
|
| Publications: |
2 |
Organism: |
Neovison Vison |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
BAG2 |
0.367 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-126953 |
Ser20 |
GRFCRSSsMADRSSR |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15271996 |
We provided definite evidence that mapkapk2 phosphorylates bag2 at ser 20 in vitro and in vivo. These results demonstrate that bag2 is a novel component of the p38 mapk signaling pathways. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates
phosphorylation
|
ZFP36L1 |
0.619 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-161270 |
Ser203 |
PRLQHSFsFAGFPSA |
Homo sapiens |
|
| pmid |
sentence |
| 18326031 |
Mk2-mediated inhibition of brf1 requires phosphorylation at s54, s92, and s203. Phosphorylation of brf1 by mk2 does not appear to alter its ability to interact with ares or to associate with mrna decay enzymes. Thus, mk2 inhibits brf1-dependent amd through direct phosphorylation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-161274 |
Ser54 |
GGFPRRHsVTLPSSK |
Homo sapiens |
|
| pmid |
sentence |
| 18326031 |
Mk2-mediated inhibition of brf1 requires phosphorylation at s54, s92, and s203. Phosphorylation of brf1 by mk2 does not appear to alter its ability to interact with ares or to associate with mrna decay enzymes. Thus, mk2 inhibits brf1-dependent amd through direct phosphorylation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-161278 |
Ser92 |
RFRDRSFsEGGERLL |
Homo sapiens |
|
| pmid |
sentence |
| 18326031 |
Mk2-mediated inhibition of brf1 requires phosphorylation at s54, s92, and s203. Phosphorylation of brf1 by mk2 does not appear to alter its ability to interact with ares or to associate with mrna decay enzymes. Thus, mk2 inhibits brf1-dependent amd through direct phosphorylation. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 |
phosphorylation
|
LSP1 |
0.636 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250147 |
Ser204 |
KLIDRTEsLNRSIEK |
in vitro |
|
| pmid |
sentence |
| 8995217 |
LSP1 is the major substrate for mitogen-activated protein kinase-activated protein kinase 2 in human neutrophils |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250148 |
Ser252 |
PKLARQAsIELPSMA |
in vitro |
|
| pmid |
sentence |
| 8995217 |
LSP1 is the major substrate for mitogen-activated protein kinase-activated protein kinase 2 in human neutrophils. . Inspection of the human LSP1 protein sequence (22) identifies two serine residues at positions 204 and 252 as potential phosphorylation sites. The results show that LSP1 is a substrate for MAPKAP kinase 2 in vitro and that the phosphorylation site(s) are located in the C-terminal 152 amino acid residues, as predicted from the sequence. |
|
| Publications: |
2 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
HSPA1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273674 |
Ser241 |
DFDNRLVsHFVEEFK |
in vitro |
|
| pmid |
sentence |
| 31642047 |
We demonstrate that MK2 phosphorylates HspA1L solely on Ser241, a residue within the N-terminal nucleotide-binding domain of the enzyme. This phosphorylation event enhances the chaperone activity of HspA1L in vitro and renders male germ cells more resistant to heat stress-induced apoptosis. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
ALOX5 |
0.544 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250143 |
Ser272 |
CSLERQLsLEQEVQQ |
in vitro |
|
| pmid |
sentence |
| 11844797 |
Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2). when stimulated with only exogenous arachidonic acid, activity for the S271A mutant was significantly lower as compared with wild type 5-LO. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPK14 | up-regulates activity
phosphorylation
|
MAPKAPK2 |
0.769 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-118036 |
Ser272 |
SNHGLAIsPGMKTRI |
Homo sapiens |
Neutrophil |
| pmid |
sentence |
| 14499342 |
Mapk-activated protein kinase-2 (mk2) is activated by p38 mapk in human neutrophils. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250101 |
Thr206 |
PNAILKLtDFGFAKE |
in vitro |
|
| pmid |
sentence |
| 7592979 |
In Vitro Activation of MAPKAP Kinase 2 by p38/40. the constitutively active mutant T205E,T317E shows no changes in activity after treatment with the p38/40 fraction |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-118040 |
Thr222 |
TSHNSLTtPCYTPYY |
Homo sapiens |
Neutrophil |
| pmid |
sentence |
| 14499342 |
Mapk-activated protein kinase-2 (mk2) is activated by p38 mapk in human neutrophils. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-44351 |
Thr25 |
APPPQPPtPALPHPP |
Homo sapiens |
|
| pmid |
sentence |
| 8846784 |
Here we show that in vitro rk phosphorylates human gst-mapkap kinase-2 at thr25 in the proline-rich n-terminal region thr222 and ser272 in the catalytic domain and thr334 in the c-terminal domain. Using novel methodology we demonstrate that activation of mapkap kinase-2 requires the phosphorylation |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250102 |
Thr317 |
PTQRMTItEFMNHPW |
in vitro |
|
| pmid |
sentence |
| 7592979 |
In Vitro Activation of MAPKAP Kinase 2 by p38/40. the constitutively active mutant T205E,T317E shows no changes in activity after treatment with the p38/40 fraction |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-118044 |
Thr334 |
QSTKVPQtPLHTSRV |
Homo sapiens |
Neutrophil |
| pmid |
sentence |
| 14499342 |
Mapk-activated protein kinase-2 (mk2) is activated by p38 mapk in human neutrophils. |
|
| Publications: |
6 |
Organism: |
Homo Sapiens, In Vitro |
| Pathways: | FLT3-ITD signaling, P38 Signaling |
| + |
MAPK1 | up-regulates
phosphorylation
|
MAPKAPK2 |
0.509 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-44339 |
Ser272 |
SNHGLAIsPGMKTRI |
Homo sapiens |
|
| pmid |
sentence |
| 8846784 |
Using novel methodology we demonstrate that activation of mapkap kinase-2 requires the phosphorylation of any two of the three residues thr222, ser272 and thr334. gst-mapkap kinase-2 lacking the n-terminal domain was inactive, but activated fully when phosphorylated at thr222, ser272 and thr334 by p42 mapk or rk. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-44343 |
Thr222 |
TSHNSLTtPCYTPYY |
Homo sapiens |
|
| pmid |
sentence |
| 8846784 |
Using novel methodology we demonstrate that activation of mapkap kinase-2 requires the phosphorylation of any two of the three residues thr222, ser272 and thr334. gst-mapkap kinase-2 lacking the n-terminal domain was inactive, but activated fully when phosphorylated at thr222, ser272 and thr334 by p42 mapk or rk. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-44347 |
Thr334 |
QSTKVPQtPLHTSRV |
Homo sapiens |
|
| pmid |
sentence |
| 8846784 |
Using novel methodology we demonstrate that activation of mapkap kinase-2 requires the phosphorylation of any two of the three residues thr222, ser272 and thr334. gst-mapkap kinase-2 lacking the n-terminal domain was inactive, but activated fully when phosphorylated at thr222, ser272 and thr334 by p42 mapk or rk. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
LIMK1 |
0.361 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-144333 |
Ser323 |
KDLGRSEsLRVVCRP |
Homo sapiens |
|
| pmid |
sentence |
| 16456544 |
Mk2 activated limk1 by phosphorylation at ser-323. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
PLK1 |
0.35 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-179968 |
Ser326 |
LTIPPRFsIAPSSLD |
Homo sapiens |
|
| pmid |
sentence |
| 18695677 |
Here, we have identified mk2 as a major plk1 kinase toward ser326, whose phosphorylation is critical to recruit ?-Tubulin to centrosomes and subsequent establishment of functional bipolar spindles. To our knowledge, this is the first direct evidence to demonstrate that the essential function of plk1 in centrosome maturation and bipolar spindle formation is controlled by its upstream kinase. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
LPCAT2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263077 |
Ser34 |
PMVPRQAsFFPPPVP |
Mus musculus |
RAW/LR5 Cell |
| pmid |
sentence |
| 20663880 |
Mass spectrometry and mutagenesis analyses identified Ser34 of LPCAT2 as the phosphorylation site to enhance the catalytic activities. The experiments using inhibitors and siRNA against MAPK cascades demonstrated that LPCAT2 phosphorylation through LPS-TLR4 signaling may directly depend on MAPK-activated protein kinase 2 (MAPKAP kinase 2 or MK2). |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
MAPKAPK2 |
phosphorylation
|
EEF2K |
0.352 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249710 |
Ser377 |
PPLLRPLsENSGDEN |
in vitro |
|
| pmid |
sentence |
| 12171600 |
Identification of Ser-377 as the site on eEF2 kinase phosphorylated in vitro by MAPKAP-K2, MAPKAP-K3 and MAPKAP-K5. Maximal phosphorylation of eEF2 kinase by MAPKAP-K2 (Figure 5) or MAPKAP-K5 (results not shown) had no effect on its activity. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
AGO2 |
0.437 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166615 |
Ser387 |
SKLMRSAsFNTDPYV |
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Mk2 was found to phopsphorylate in vitro the ago2 protein in ser387, which was identified as the major ago2 phosphorylation site in cells.and mutation of ser387 to alanineimpairsago2 localization to therna-containing granules termedprocessing bodies |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates quantity
phosphorylation
|
CEP131 |
0.292 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278181 |
Ser47 |
KPIVRSVsVVTGSEQ |
Homo sapiens |
|
| pmid |
sentence |
| 26616734 |
We identify CEP131 as a major Centriolar satellites-associated substrate of p38-dependent, MK2-mediated phosphorylation on two defined residues and show that these modifications promote binding to 14-3-3 proteins, in turn leading to cytoplasmic sequestration of CEP131 and associated Centriolar satellites factors.|We therefore conclude that MK2 dependent phosphorylation of CEP131 at S47 and S78 and the ensuing binding of 14-3-3 proteins play an essential role in triggering stress induced remodelling of CS. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278182 |
Ser78 |
NNLRRSNsTTQVSQP |
Homo sapiens |
|
| pmid |
sentence |
| 26616734 |
MK2 directly phosphorylates two residues on CEP131, S47 and S78, to create dual binding sites for 14-3-3 proteins.|We identify CEP131 as a major Centriolar satellites-associated substrate of p38-dependent, MK2-mediated phosphorylation on two defined residues and show that these modifications promote binding to 14-3-3 proteins, in turn leading to cytoplasmic sequestration of CEP131 and associated Centriolar satellites factors. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
PIAS1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199944 |
Ser522 |
DTSYINTsLIQDYRH |
Homo sapiens |
|
| pmid |
sentence |
| 23202365 |
T he mitogen-activated protein kinase (mapk)-activated protein kinase-2 is a proinflammatory kinase and phosphorylates pias1 at the ser522 residue. Activation of mapk-activated protein kinase-2 enhances p53-sumoylation, but a pias1 phosphorylation mutant, pias1-s522a, abolished this p53-sumoylation, suggesting a critical role for pias1-s522 phosphorylation in its sumo ligase activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Skin |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
TRIM29 |
0.308 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273675 |
Ser550 |
GYPSLMRsQSPKAQP |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 24469230 |
ATDC was phosphorylated directly by MAPKAP kinase 2 (MK2) at Ser550 in an ATM-dependent manner. Phosphorylation at Ser-550 by MK2 was required for the radioprotective function of ATDC. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates
phosphorylation
|
PARN |
0.376 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-168377 |
Ser557 |
NHYYRNNsFTAPSTV |
Homo sapiens |
|
| pmid |
sentence |
| 20932473 |
Mk2 phosphorylates parn, blocking gadd45_ mrna degradation. Parn can serve as a direct substrate for mk2, and demonstrating that ser-557 is the dominant mk2 phosphorylation site. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates activity
phosphorylation
|
YWHAZ |
0.623 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250151 |
Ser58 |
VVGARRSsWRVVSSI |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 12861023 |
We confirmed that MAPKAPK2 interacted with and phosphorylated 14-3-3zeta in vitro and in HEK293 cells. Mutation analysis showed that MAPKAPK2 phosphorylated 14-3-3zeta at Ser-58. S58D mutation significantly impaired both 14-3-3zeta dimerization and binding to Raf-1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 |
phosphorylation
|
ARPC5 |
0.352 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250144 |
Ser77 |
AVKDRAGsIVLKVLI |
in vitro |
|
| pmid |
sentence |
| 12829704 |
MAPKAPK2 also phosphorylated p16-Arc in intact Arp2/3 complexes precipitated from neutrophil lysates. Mutation of serine-77 to alanine on the A isoform prevented phosphorylation by MAPKAPK2. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
HNRNPA0 |
0.533 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262951 |
Ser84 |
VELKRAVsREDSARP |
Mus musculus |
Macrophage |
| pmid |
sentence |
| 12456657 |
MAPKAP-K2 phosphorylated hnRNP A0 at Ser84 in vitro and this residue became phosphorylated in LPS-stimulated cells. The simplest explanation for these findings is that the phosphorylation of hnRNP A0 at Ser84 by MAPKAP-K2 enhances binding to the AREs of these mRNAs or allows hnRNP A0 to displace another protein(s) from the AREs. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
BECN1 |
0.433 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278342 |
Ser90 |
IPPARMMsTESANSF |
Homo sapiens |
|
| pmid |
sentence |
| 25693418 |
Beclin 1 S90 is phosphorylated by MAPKAPK2 (MK2) and MAPKAPK3 (MK3). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
AATF |
0.328 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-191935 |
Thr366 |
FTVYRNRtLQKWHDK |
Homo sapiens |
|
| pmid |
sentence |
| 22909821 |
Upon genotoxic stress, aatf is phosphorylated by the checkpoint kinase mk2. Phosphorylation results in the release of aatf from cytoplasmic mrlc3 and subsequent nuclear translocation where aatf binds to the puma, bax and bak promoter regions to repress p53-driven expression of these pro-apoptotic genes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPK3 | up-regulates
phosphorylation
|
MAPKAPK2 |
0.477 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-201687 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23583303 |
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-121994 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14967450 |
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
SRF |
0.582 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166640 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Neverthless, some transcription factors, such as e47, er81, srf and creb are also phosphorylated by mk2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
TCF3 |
0.519 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166643 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Neverthless, some transcription factors, such as e47, er81, srf and creb are also phosphorylated by mk2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
CASP3 |
0.311 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278960 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 35554104 |
MK2 Phosphorylates Caspase-3, Facilitates Nuclear Translocation of Caspase 3, and Regulates Apoptosis.|Over-expression of MK2 led to an increase in nuclear caspase-3 activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | down-regulates
phosphorylation
|
CDC25A |
0.365 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-152996 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17292828 |
Mk2 was required for the degradation of cdc25a. Mk2 phosphorylates cdc25a in vitro. Phosphorylation of cdc25a in vivo has been shown to facilitate its ubiquitin-mediated proteolysis |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
ELAVL1 |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166622 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Mk2 and mk3 participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating the are-binding proteins ttp and hur, and by regulating eef2k |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Gbeta | up-regulates
phosphorylation
|
MAPKAPK2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270117 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14967450 |
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates
phosphorylation
|
ETV1 |
0.61 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-166625 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20626350 |
Neverthless, some transcription factors, such as e47, er81, srf and creb are also phosphorylated by mk2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ERK1/2 | up-regulates
phosphorylation
|
MAPKAPK2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270206 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14967450 |
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | FLT3-ITD signaling |
4.0
MAPKAPK2
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