| + |
HACE1 | down-regulates quantity 
ubiquitination
|
RAC1 |
0.359 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278810 |
Lys147 |
PQGLAMAkEIGAVKY |
Homo sapiens |
|
| pmid |
sentence |
| 23864022 |
GTP-bound Rac1 is ubiquitylated by Hace1 at lysine (K)-147 (ref. xref ).|This indicates that Hace1 binds and targets Rac1 once the latter has been recruited to the complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKT | down-regulates activity
phosphorylation
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248036 |
Ser71 |
YDRLRPLsYPQTDVF |
Homo sapiens |
SK-MEL-2 Cell |
| pmid |
sentence |
| 10617634 |
The results suggest that Akt kinase of the phosphoinositide 3-kinase signal transduction pathway phosphorylates serine 71 of Rac1 as one of its authentic substrates and modulates the Rac1 signal transduction pathway through phosphorylation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT/FLT3 |
| + |
AKT1 | down-regulates activity
phosphorylation
|
RAC1 |
0.736 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252576 |
Ser71 |
YDRLRPLsYPQTDVF |
Homo sapiens |
Melanoma Cell |
| pmid |
sentence |
| 10617634 |
Akt protein kinase inhibits Rac1-GTP binding through phosphorylation at serine 71 of Rac1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SRC | up-regulates activity 
phosphorylation
|
RAC1 |
0.632 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-280132 |
Tyr64 |
DTAGQEDyDRLRPLS |
Homo sapiens |
|
| pmid |
sentence |
| 30544910 |
In addition, Rac1 is phosphorylated at Y64 by FAK and Src kinases ( xref ); Y64 phosphorylation targets Rac1 to focal adhesions. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Axon guidance |
| + |
FAM13A | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.456 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260502 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP27 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.604 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260482 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VAV1 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.774 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260581 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259081 |
|
|
Drosophila melanogaster |
|
| pmid |
sentence |
| 23525006 |
We identify the GTP exchange factor (GEF) Vav as a key regulator of Rac activity downstream of RTKs in a developmentally regulated cell migration event |
|
| Publications: |
2 |
Organism: |
Homo Sapiens, Drosophila Melanogaster |
| + |
ARHGEF6 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.637 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272167 |
|
|
Homo sapiens |
Blood Platelet |
| pmid |
sentence |
| 26507661 |
ARHGEF6 is a Rho guanine nucleotide exchange factor for Rac1 and constitutively bound to GIT1. NO and PGI2 activate PKG and PKA, respectively and both kinases phosphorylate ARHGEF6 on Ser-684 and possibly on Ser-640. Phosphorylation of ARHGEF6 results in the assembly of a GIT1-ARHGEF6–14-3-3 complex. These changes might contribute to PGI2- and NO-mediated Rac1 inhibition. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RASGRF2 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.517 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260574 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF10 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.464 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260536 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
binding
|
ALS2 |
0.546 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277777 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 30485418 |
Thus, in our system, activeRac1 may recruit ALS2 only at the very early stage ofmacropinocytosis including membrane ruffles, suggest-ing that ALS2 is retained by some other mechanismsuntil Rab conversion. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SDC4 | up-regulates activity
binding
|
RAC1 |
0.524 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255849 |
|
|
Homo sapiens |
Satellite Cell |
| pmid |
sentence |
| 23290138 |
Rac1 is associated with Sdc4 and is activated by FN binding […] We observed that over-expression of Fzd7, or stimulation with FN resulted in increased levels of active Rac1 in primary myoblasts |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Skeletal Muscle |
| + |
RHOH | down-regulates activity
relocalization
|
RAC1 |
0.434 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259085 |
|
|
Mus musculus |
Bone Marrow Cell |
| pmid |
sentence |
| 18089848 |
Therefore, RhoH functions as a Rac1 antagonist by inhibiting Rac1 translocation to the cell plasma membrane in the regulation of cell migration and F-actin assembly of HPCs |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
OPHN1 | up-regulates activity
gtpase-activating protein
|
RAC1 |
0.607 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268399 |
|
|
Homo sapiens |
Neuron |
| pmid |
sentence |
| 12932438 |
OPHN-1 colocalized with the actin cytoskeleton in neuronal and glial cells. We have previously shown that OPHN1 stimulates GTPases activity of RhoA, Cdc42, and Rac1 in vitro |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF7 | up-regulates 
|
RAC1 |
0.651 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-155744 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17562871 |
We propose that the association of plcgamma1 with complexes containing git1 and beta-pix is essential for its role in integrin-mediated cell spreading and motility. As a component of this complex, plcgamma1 is also involved in the activation of cdc42 and rac1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGDIA | down-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.818 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268436 |
|
|
Rattus norvegicus |
Glioma Cell |
| pmid |
sentence |
| 20696765 |
Here, we report the expression of plexin-B3 in glioma cells, which upon stimulation by its ligand Sema5A results in significant inhibition of cell migration and invasion. A search for the underlying mechanism revealed direct interaction of plexin-B3 with RhoGDP dissociation inhibitor α (RhoGDIα), a negative regulator of RhoGTPases that blocks guanine nucleotide exchange and sequesters them away from the plasma membrane. direct interaction of RhoGDIα and the cytoplasmic domain of plexin-B3 (plexin-B3CD) was confirmed by GST pulldown assays.RhoGDIα is required for Sema5A-induced Rac1 inactivation and inhibition of cell invasion in C6 glioma. |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| Pathways: | Axon guidance |
| + |
SRC | up-regulates
phosphorylation
|
RAC1 |
0.632 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-158954 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17991704 |
N attractive hypothesis consistent with our present data is that the gef responsible for rac activation in mce cells may be activated by src family kinase tyrosine phosphorylationour results present a novel mechanism by which the pi3k and src signaling cascades cooperate to activate rac and promote intestinal epithelial cell migration downstream of egfr. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Axon guidance |
| + |
PTK2 | up-regulates activity
phosphorylation
|
RAC1 |
0.592 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279652 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22163037 |
Both Src and FAK phosphorylate Rac1 at tyrosine 64.|Our investigations of direct interactions between Rac1, Src, and FAK were motivated by our previous insights into FAK augmentation of Rac1 activation during cell spreading, and the Cerione lab 's work on the interactions between Src and Cdc42 , . |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Axon guidance |
| + |
MCF2 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.679 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260557 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP20 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.459 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260473 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates
binding
|
MAPK8 |
0.673 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-152808 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17251915 |
The mechanism by which pak1 induced cancer growth might involve activation of jnk in the non-canonical wnt pathway, frizzled uses galfaq or galfai and gbetagamma dimers to activate phospholipase c (plc), resulting in protein kinase c (pkc) activation and calcium mobilization that regulates the transcription factor nfat, and frizzled also signals through the small gtpases rho and rac to c-jun n-terminal kinase (jnk), which activates the ap1 transcription factor. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
RAC1 | up-regulates
|
MAPK14 |
0.555 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-174602 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21712438 |
Hypertonicity activates p38 via a rac1-osm-mekk3-mkk3-p38 pathway. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP9 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.623 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260464 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PREX1 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.73 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260569 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DOCK6 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.546 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275670 |
|
|
|
|
| pmid |
sentence |
| 23462102 |
Dock6 is a guanine nucleotide exchange factor (GEF) that activates the Rho family guanosine triphosphatases Rac1 and Cdc42 to regulate the actin cytoskeleton. |
|
| Publications: |
1 |
| + |
TIAM1 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.761 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260577 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ELMO2 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.6 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266821 |
|
|
Mus musculus |
N1E-115 Cell |
| pmid |
sentence |
| 25533347 |
We found in this study that AUTS2 is involved in Rac1 activation via P-Rex1 and the Elmo2/Dock180 complex, but not STEF or Tiam1, for the lamellipodia formation in N1E-115 cells. However, the enhancement of neurite elongation in primary neurons by AUTS2 expression is specifically mediated by the Elmo2/Dock180 complex. These results suggested that several Rac-GEFs differentially or cooperatively participate in Rac1 activation to promote neuronal migration and neurite outgrowth. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
DOCK1 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.745 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266822 |
|
|
Mus musculus |
N1E-115 Cell |
| pmid |
sentence |
| 25533347 |
We found in this study that AUTS2 is involved in Rac1 activation via P-Rex1 and the Elmo2/Dock180 complex, but not STEF or Tiam1, for the lamellipodia formation in N1E-115 cells. However, the enhancement of neurite elongation in primary neurons by AUTS2 expression is specifically mediated by the Elmo2/Dock180 complex. These results suggested that several Rac-GEFs differentially or cooperatively participate in Rac1 activation to promote neuronal migration and neurite outgrowth. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
PIK3CA | up-regulates
|
RAC1 |
0.585 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-175238 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21779497 |
Pi3k can also activate rac, and this activation is involved in cytoskeleton reorganization. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
binding
|
GIT1 |
0.669 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277783 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 39000072 |
Activated RAC1 interacts with GIT1, a GAP protein of ARF6, and causes the inactivation of ARF6 [78]. As ARF6 plays a role in the promotion of the recycling of macropinosomes to the plasma membrane [78], the inactivation of ARF6 by RAC1 reduces the recycling of macropinosomes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DVL1 | up-regulates activity
binding
|
RAC1 |
0.599 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-185274 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19365405 |
B-catenin-independent wnt signaling can activate rho family gtpases through at least two mechanisms: (1) direct activation of rac1 by dvl;and (2) activation of rhoa via dvl-associated activator of morphogenesis-1 (daam1), possibly through the weak-similarity guaninenucleotide exchange factor (wgef)1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
ARHGAP22 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.548 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260477 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
binding
|
PAK1 |
0.798 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248236 |
|
|
Mus musculus |
Brain |
| pmid |
sentence |
| 8107774 |
A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ARHGAP35 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.734 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260493 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
binding
|
MAPK8 |
0.673 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-195414 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22252525 |
The mechanism by which pak1 induced cancer growth might involve activation of jnk in the non-canonical wnt pathway, frizzled uses galfaq or galfai and gbetagamma dimers to activate phospholipase c (plc), resulting in protein kinase c (pkc) activation and calcium mobilization that regulates the transcription factor nfat, and frizzled also signals through the small gtpases rho and rac to c-jun n-terminal kinase (jnk), which activates the ap1 transcription factor. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
BCR | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.615 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260526 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
|
MAPK8 |
0.673 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199539 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23151663 |
Planar cell polarity (pcp) signalling triggers activation of the small gtpases rhoa and rac1, which in turn activate rho kinase (rock) and jun-n-terminal kinase (jnk), respectively, leading to actin polymerization and microtubule stabilization. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
RAC1 | up-regulates activity
|
Proliferation |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255648 |
|
|
|
|
| pmid |
sentence |
| 23290138 |
This result strongly supports the assertion that Wnt7a and FN stimulate PCP signaling to drive the symmetric expansion of satellite stem cells during regenerative myogenesis. |
|
| Publications: |
1 |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3 |
| + |
GNAI1 | up-regulates activity
binding
|
RAC1 |
0.49 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256530 |
|
|
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 11099498 |
These findings indicate that both G alpha(i) and G beta gamma stimulate Rac and Cdc42 pathways with lysophosphatidic acid-induced cell spreading on fibronectin |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
FAM13B | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.456 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260503 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TGFBR1 | up-regulates activity
|
RAC1 |
0.277 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-227496 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19726546 |
Thus, TGF-_1 rapidly stimulates activity of both RhoA and Rac1 and this activation requires ALK5/T_RI kinase activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VAV2 | up-regulates
guanine nucleotide exchange factor
|
RAC1 |
0.771 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-81645 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10982832 |
Vav2 activates rac1 / vav2 is an exchange factor for rho family gtpases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SRGAP2 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.603 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260516 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP31 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.582 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260489 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP33 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.465 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260491 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SH3BP1 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.371 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260514 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TIAM1 | up-regulates
binding
|
RAC1 |
0.761 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-94691 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12393875 |
Lpa-induced rac activation requires tiam1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VAV2 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.771 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260583 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DAAM1 | up-regulates activity
|
RAC1 |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199381 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23151663 |
In pcp, dvl binds to proteins such as pkc, atypical pkc (apkc), dvl?associated Activator of morphogenesis 1 (daam1), dvl-associating protein with a high frequency of leu residues (daple) and partitioning defective 6 (par6), which are important for the regulation of small gtpases such as rho and rac and, consequently, the cytoskeleton and cell polarity58. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
ARAP3 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.49 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260456 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FLT3 | up-regulates activity
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261536 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 18192505 |
Inhibition of FLT3/ITD leads to a small decrease in RAC1 activity |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT/FLT3 |
| + |
DVL1 | up-regulates activity
|
RAC1 |
0.599 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199384 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23151663 |
In pcp , dvl binds to proteins such as pkc, atypical pkc (apkc), dvl associated activator of morphogenesis 1 (daam1), dvl-associating protein with a high frequency of leu residues (daple) and partitioning defective 6 (par6), which are important for the regulation of small gtpases such as rho and rac and, consequently, the cytoskeleton and cell polarity58. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
SRGAP3 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.583 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260518 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FGD5 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260555 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SRGAP1 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.577 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260515 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DOCK3 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.671 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260548 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PLEKHG6 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.293 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260568 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP4 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.499 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260460 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PLCG1 | up-regulates
|
RAC1 |
0.556 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-155747 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17562871 |
We propose that the association of plcgamma1 with complexes containing git1 and beta-pix is essential for its role in integrin-mediated cell spreading and motility. As a component of this complex, plcgamma1 is also involved in the activation of cdc42 and rac1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SYDE2 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.425 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260522 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SRGAP3 | down-regulates 
|
RAC1 |
0.583 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-95918 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12447388 |
Wrp binds directly to wave-1 through its src homology domain 3 and specifically inhibits rac function in vivo. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP30 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.426 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260486 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
phosphatidic acid | up-regulates
chemical activation
|
RAC1 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-178632 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18480413 |
The c-terminal polybasic motif of rac1 is responsible for direct interaction with pa, |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CTNND1 | up-regulates
binding
|
RAC1 |
0.603 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-198938 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22946057 |
We demonstrate that p120-catenin participates in the stimulation of rac1 activity, binding directly to this protein. In addition we show that vav2 also binds to p120-catenin and is required for rac1 activation and for beta-catenin translocation to the nucleus.Vav2 And rac1 association with p120-catenin was modulated by phosphorylation of this protein, which was stimulated upon serine/threonine phosphorylation by ck1 and inhibited by tyrosine phosphorylation by src or fyn |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TIAM2 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.606 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260578 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP17 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.581 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272166 |
|
|
Homo sapiens |
Blood Platelet |
| pmid |
sentence |
| 26507661 |
ARHGAP17 is a Rho GTPase-activating protein of Rac1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates
binding
|
MAPK9 |
0.542 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-178265 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18423204 |
We show that rac1 activates jnk2 that in turn phosphorylates beta-catenin on critical residues and controls its nuclear translocation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates
|
Proliferation |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259088 |
|
|
Homo sapiens |
NCI-H1703 Cell, A-549 Cell |
| pmid |
sentence |
| 22549160 |
The small GTPase Rac1 regulates many cellular processes, including cytoskeletal reorganization, cell migration, proliferation, and survival. We found that silencing of Rac1 expression decreases NSCLC migration and proliferation [.] |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT Signaling, WNT/FLT3 |
| + |
SDC1 | up-regulates activity
binding
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-282625 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30918400 |
To identify Sdc1 domains required for Rac1 activation and macropinocytosis, we constructed a series of Sdc1 truncation mutants24,25 that were expressed on the cell surface, except for soluble Sdc1 protein, which was detected in culture medium because it lacks the transmembrane and intra-cellular domains (Extended Data Fig.9a, b). As expected, expression of full-length Sdc1 fully rescued Rac1 activity in Sdc-depleted cells. In contrast, soluble Sdc1 failed to reactivate Rac1 (Extended Data Fig.9c), indicating the membrane localization of SDC1 is essential for macropinocytosis. Deletion of Sdc1 extra-cellular domain (ΔEct) also abolished its activity toward Rac1. However, the GAG mutant with mutated heparan sulfate modification sites exerted a partial rescue effect (Extended Data Fig.9c), suggesting heparin sulfate may not be essential for this function. Interestingly, the C30 mutant (deletion of the C-terminal 30 amino acids) also failed to rescue Rac1 activity in Sdc1-depleted cells (Extended Data Fig.9c) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PREX2 | up-regulates activity
guanine nucleotide exchange factor, catalytic activity
|
RAC1 |
0.625 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260571 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259191 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25829446 |
Here, we used cell biology, biochemistry, and genetic approaches to show that PTEN suppresses cell movement by blocking PREX2 GEF–catalyzed activation of the GTPase RAC1. PTEN binds PREX2 and directly inhibits GEF activity. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
RAP1GDS1 | up-regulates
binding
|
RAC1 |
0.503 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-171418 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21242305 |
Smggds has been previously shown to activate a wide variety of small gtpases, including the ras family members rap1a, rap1b, and k-ras, as well as the rho family members cdc42, rac1, rac2, rhoa, and rhob |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RACGAP1 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.604 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260512 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
IQGAP1 | down-regulates activity
binding
|
RAC1 |
0.726 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261889 |
|
|
|
|
| pmid |
sentence |
| 15695813 |
Although the name implies that it functions as a GTPase-activating protein, IQGAP1 actually stabilizes Cdc42 and Rac1 in the active, GTP-bound form (5, 8, 17). Thus, IQGAP1 acts as an “anti-GTPase-activating protein” for Cdc42 and Rac1, with marked effects on the cytoskeleton. |
|
| Publications: |
1 |
| + |
FZD7 | up-regulates activity
|
RAC1 |
0.476 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255647 |
|
|
|
|
| pmid |
sentence |
| 23290138 |
We observed that overexpression of Fzd7 or stimulation with FN resulted in increased levels of active Rac1 in primary myoblasts |
|
| Publications: |
1 |
| + |
TAGAP | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.426 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260524 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAB7A | up-regulates activity
binding
|
RAC1 |
0.274 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261304 |
|
|
Rattus norvegicus |
|
| pmid |
sentence |
| 16040606 |
Rab7-Rac1 interaction may mediate late endosomal transport between microtubules and microfilaments |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| + |
LRRK1 | up-regulates activity
phosphorylation
|
RAC1 |
0.287 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279626 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27600824 |
In vitro kinase assays confirmed that recombinant Lrrk1 phosphorylated RAC1-GST protein, and immunoprecipitation showed that the interaction of Lrrk1 with RAC1 occurred within 10 min after RANKL treatment.|Lrrk1 phosphorylates and activates RAC1 and Cdc42 small GTPase proteins in osteoclasts. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TIAM1 | up-regulates
|
RAC1 |
0.761 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-167073 |
|
|
Homo sapiens |
Neuron |
| pmid |
sentence |
| 20654717 |
This smo-tiam1 complex dissociates upon shh-mediated activation of smo, thus allowing tiam1 to activate rac1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Brain |
| + |
RAC1 | up-regulates activity
binding
|
PAK2 |
0.759 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248250 |
|
|
Mus musculus |
Brain |
| pmid |
sentence |
| 8107774 |
A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ARHGAP23 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.451 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260480 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARAP2 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.483 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260454 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP12 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260469 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CHN1 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.678 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260499 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DOCK4 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.602 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266823 |
|
|
Mus musculus |
Neuro-2a Cell |
| pmid |
sentence |
| 32009906 |
DOCK4 (dedicator for cytokinesis 4), a guanine nucleotide exchange factor (GEF) for the small GTPase Rac1, is one of few genes that are associated with both ASD and dyslexia. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
RALBP1 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.597 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260513 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
cholesterol | up-regulates quantity
relocalization
|
RAC1 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277761 |
|
|
Homo sapiens |
Pancreatic Cancer Cell |
| pmid |
sentence |
| 31827278 |
Accumulation of V-ATPase at the plasma membrane is necessary for the cholesterol-dependent plasma-membrane association of RAC1, a prerequisite for the stimulation of membrane ruffling and macropinocytosis. In line with these observations, immunohistochemical staining of V-ATPase in human pancreatic ductal adenocarcinoma (PDAC) specimens revealed prominent staining at the cell periphery in neoplastic lesions, in con- trast to the predominantly cytoplasmic staining observed in adjacent normal tissues (Fig. 2e). Thus, mutant RAS-dependent plasma mem- brane V-ATPase displayed preferential accumulation in membrane ruffles, consistent with patterns observed in invasive breast, melanoma and pancreatic cancer cells |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MVD | up-regulates activity
lipidation
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265892 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 25378391 |
Akt modulated the pathway by phosphorylating mevalonate diphosphate decarboxylase (MDD) at Ser96. These data suggest that Akt regulates Rac1 activity by directly phosphorylating MDD at Ser96, which augments Rac1 geranylgeranylation. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
RAC1 | up-regulates
relocalization
|
USP6 |
0.332 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-98938 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12612085 |
In quiescent cells, tre17 is localized to intracellular filamentous and punctate structures in the cytoplasm, folded in an inactive conformation. Upon growth factor addition, cdc42 and rac1 become activated and recruit tre17 to the plasma membrane. Stable membrane localization of tre17 also requires polymerized actin. This recruitment process leads to a conformational change in tre17, such that the n-terminal portion of the molecule further stimulates the accumulation of cortical actin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP39 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.521 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260494 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates
binding
|
BAIAP2 |
0.747 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-85302 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11130076 |
Here we demonstrate that irsp53, a substrate for insulin receptor with unknown function, is the 'missing link' between rac and wave. Activated rac binds to the amino terminus of irsp53, and carboxy-terminal src-homology-3 domain of irsp53 binds to wave to form a trimolecular complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Axon guidance |
| + |
ARHGEF25 | up-regulates
guanine nucleotide exchange factor
|
RAC1 |
0.636 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236882 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 16314529 |
Exogenous expression of geft promotes myogenesis ofc2c12 cells via activation of rhoa, rac1, and cdc42 and their downstream effector proteins, while a dominant negative mutant of geft inhibits this process. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
DOCK7 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.562 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261887 |
|
|
Mus musculus |
Blood Platelet |
| pmid |
sentence |
| 29187380 |
As a GEF, Dock7 exchanges GDP for GTP on Cdc42 and Rac1, causing their activation, followed by activation of downstream effectors, including the dephosphorylation (activation) of cofilin, a key regulator of actin turnover. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
MYO9B | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.559 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260510 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
|
WAVE complex |
0.639 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261877 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27871158 |
Cdc42 can induce Arp2/3-mediated filopodia formation through the activation of WASp (Wiskott-Aldrich syndrome proteins) and neuronal N-WASp (Rohatgi et al., 1999). Similarly, Rac1-enhanced lamellipodia formation is related to Arp2/3 activation by the WAVE (WASP-family verprolin-homologous) complex |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PARD6A | up-regulates
|
RAC1 |
0.626 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199530 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23151663 |
In pcp , dvl binds to proteins such as pkc, atypical pkc (apkc), dvl?associated Activator of morphogenesis 1 (daam1), dvl-associating protein with a high frequency of leu residues (daple) and partitioning defective 6 (par6), which are important for the regulation of small gtpases such as rho and rac and, consequently, the cytoskeleton and cell polarity58. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
binding
|
BAIAP2 |
0.747 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265554 |
|
|
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 19171758 |
In this study, we report that Kank disrupts the function of active Rac1 through IRSp53. The binding between IRSp53 and Kank inhibits the association of active Rac1 with IRSp53 rather than the association of active cdc42 with IRSp53. Kank inhibits the formation of lamellipodia and membrane ruffles induced by active Rac1 in NIH3T3 cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Axon guidance |
| + |
DRAM2 | up-regulates quantity by expression
transcriptional regulation
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259141 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30755245 |
Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). Furthermore, DRAM2 overexpression increased the expression of proteins RAC1, RHOA, RHOC, ROCK1, and decreased RHOB expression, all of which are cell migration factors. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AMPK | up-regulates activity
|
RAC1 |
0.26 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277766 |
|
|
Homo sapiens |
Pancreatic Cancer Cell |
| pmid |
sentence |
| 29572236 |
The discovery that AMPK activation is a general requirement for macropinosome formation in cancer cells dramatically extends our understanding of the regulation of this process. By increasing RAC1-GTP levels, AMPK activation stimulates macropinosome formation (Fig. 2I–K; Supplementary Fig. S5). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia |
| + |
ARHGAP44 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.604 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260498 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DVL3 | up-regulates
binding
|
RAC1 |
0.488 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-97409 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12533515 |
Wnt/fz activation of rac and rho is inhibited by rac-n17 and rho-n19, respectively (figs. _(figs.1d,1d, _d,5c,d;5c,d;habas et al. 2001), and requires different dvl domains wnt signaling induces complex formation between dvl and rac. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CHN2 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.653 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260500 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PI3K | up-regulates
|
RAC1 |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252707 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21779497 |
Pi3k can also activate rac, and this activation is involved in cytoskeleton reorganization. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Acute Myeloid Leukemia, FLT3-ITD in AML, WNT/FLT3 |
| + |
ARHGAP15 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.596 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260470 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MTSS1 | up-regulates
binding
|
RAC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-141573 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16280553 |
Mim-b binds and activates rac via its irsp53/mim domain |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | form complex 
binding
|
Phagocyte NADPH oxidase complex |
0.618 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277631 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 37263099 |
NADPH oxidase is composed of essential protein components in its active state: membranous subunits, including p22-phox and gp91-phox, and cytoplasmic subunits, including p47-phox, p67-phox, p40-phox, and RAC2 (in neutrophils), among which NCF4 encodes the cytoplasmic subunit p40-phox |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDH4 | up-regulates activity
|
RAC1 |
0.271 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253103 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 18701479 |
Together, these data suggest that R-cadherin expression inhibits myogenesis and induces myoblast transformation through Rac1 activation. Therefore, the properties of R-cadherin make it an attractive target for therapeutic intervention in RMS. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
RAC1 | up-regulates activity
binding
|
ROCK1 |
0.396 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-258972 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27571105 |
Although there are other activators of PCP, Wnt5a can activate the PCP pathway by forming a complex with Fzd and Ror2 receptors, activating DVL, which in turn activates Rho-family small GTPases, including RhoA and Rac, and their downstream effectors, Rho-associated protein kinase (ROCK), the actin-binding protein, Filamin A and c-Jun N-terminal protein kinase (JNK) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
ARAP1 | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.476 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260451 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DOCK10 | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.54 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260549 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GMIP | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.466 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260506 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRIO | up-regulates activity
guanine nucleotide exchange factor
|
RAC1 |
0.702 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260579 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HACE1 | down-regulates quantity by destabilization
ubiquitination
|
RAC1 |
0.359 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255538 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22036506 |
The CNF1 toxin of pathogenic Escherichia coli addresses Rac1 to ubiquitin-proteasome system (UPS). We report the essential role of the tumor suppressor HACE1, a HECT-domain containing E3 ubiquitin-ligase, in the targeting of Rac1 to UPS. HACE1 binds preferentially GTP-bound Rac1 and catalyzes its polyubiquitylation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAC1 | up-regulates activity
binding
|
PAK |
0.798 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248256 |
|
|
Mus musculus |
Brain |
| pmid |
sentence |
| 8107774 |
A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ABR | down-regulates activity
gtpase-activating protein
|
RAC1 |
0.523 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260525 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
RAC1
- 
- 