Relation Results

Summary

Name PAK1
Full Name Serine/threonine-protein kinase PAK 1
Synonyms Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK
Primary ID Q13153
Links - -
Type protein
Relations 102
Pathways EGFR Signaling, Macropinocytosis , T cell activation
Function Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskel ...
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Type: Score: Layout: SPV 
0.5990.20.3730.3930.20.4580.2510.4130.3830.20.3910.3910.3940.3360.3410.20.20.3630.4670.7840.410.420.6530.20.5670.2690.5480.5870.20.6980.2730.5350.6330.20.3070.3970.3540.5210.5440.3740.3390.20.3840.3580.20.2480.5450.5230.4090.20.3970.3420.3620.3480.60.20.3880.00.7740.20.9410.3020.20.7830.20.70.5670.20.540.20.4230.00.7PAK1ARHGDIAH3C1PREX2KIF2CPGAM1TBCBNET1CTBP1STMN1GNAZCDK11ACyclinD3/CDK11ACyclinD3/CDK11BCDK19PPM1APPP2CBPPP2CAELF3FLNASNAI1ILKPXNITGB3BPMAP2K1ARAFESR1RAF1HACE1CTTNFXR1PLK1NF2VIMPPM1EPPM1FSYN1MAP3K1CTNNB1MORC2BADSORT1DYNLL1ARHGEF2ATG5STK11ARPC1BCDK5MAPK1PA2G4AURKAMAPK3PDPK1PGM1LIMK1NLRP3OXSR1EPHA3RAC2GbetaCDC42WEE1ERK1/2RAC1BCL6NCK1MEK1/2PGAMMYLKHistone H3CRIPAKAJUBAF-actin_assembly

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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ PAK1down-regulates img/direct_inhibition.png phosphorylation ARHGDIA 0.599
Identifier Residue Sequence Organism Cell Line
SIGNOR-126650 Ser101 LESFKKQsFVLKEGV Homo sapiens
pmid sentence
Pak1 binds and phosphorylates rhogdi both in vitro and in vivo at ser101 and ser174. This resulted in dissociation of rac1-rhogdi, but not rhoa-rhogdi, complexes, as determined by in vitro assays of complexation and in vivo by coimmunoprecipitation analysis. We observed that cdc42-induced rac1 activation is inhibited by expression of pak1 autoinhibitory domain. The dissociation of rac1 from rhogdi and its subsequent activation stimulated by pdgf or egf is also attenuated by pak1 autoinhibitory domain, and this is dependent on the ability of rhogdi to be phosphorylated at ser101/174.
Identifier Residue Sequence Organism Cell Line
SIGNOR-126654 Ser174 KGMLARGsYSIKSRF Homo sapiens
pmid sentence
Pak1 binds and phosphorylates rhogdi both in vitro and in vivo at ser101 and ser174. This resulted in dissociation of rac1-rhogdi, but not rhoa-rhogdi, complexes, as determined by in vitro assays of complexation and in vivo by coimmunoprecipitation analysis. We observed that cdc42-induced rac1 activation is inhibited by expression of pak1 autoinhibitory domain. The dissociation of rac1 from rhogdi and its subsequent activation stimulated by pdgf or egf is also attenuated by pak1 autoinhibitory domain, and this is dependent on the ability of rhogdi to be phosphorylated at ser101/174.
Publications: 2 Organism: Homo Sapiens
+ PAK1 img/unknown.png phosphorylation H3C1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-91050 Ser11 TKQTARKsTGGKAPR Homo sapiens
pmid sentence
Histone h3 is a substrate of pak1 both in vitro and in vivo, and it specifically interacted with pak1 but not pak2 or pak3. Site-directed mutagenesis indicated that pak1 phosphorylates histone h3 on ser10.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation PREX2 0.373
Identifier Residue Sequence Organism Cell Line
SIGNOR-277181 Ser1107 DTISNRDsYSDCNSN Homo sapiens HEK-293 Cell
pmid sentence
P21-activated Kinases (PAKs) Mediate the Phosphorylation of PREX2 Protein to Initiate Feedback Inhibition of Rac1 GTPase. PAK-mediated phosphorylation of PREX2 reduced GEF activity toward Rac1 by inhibiting PREX2 binding to PIP3 and Gβγ.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates img/direct_inhibition.png phosphorylation KIF2C 0.393
Identifier Residue Sequence Organism Cell Line
SIGNOR-199080 Ser111 KESLRSRsTRMSTVS Homo sapiens
pmid sentence
Here we found that mcak is a cognate substrate of pak1 wherein pak1 phosphorylates mcak on serines 192 and 111 both in vivo and in vitro. Furthermore, we found that pak1 phosphorylation of mcak on serines 192 and 111 preferentially regulates its microtubule depolymerization activity and localization to centrosomes
Identifier Residue Sequence Organism Cell Line
SIGNOR-199084 Ser192 VNSVRRKsCLVKEVE Homo sapiens
pmid sentence
Here we found that mcak is a cognate substrate of pak1 wherein pak1 phosphorylates mcak on serines 192 and 111 both in vivo and in vitro. Furthermore, we found that pak1 phosphorylation of mcak on serines 192 and 111 preferentially regulates its microtubule depolymerization activity and localization to centrosomes
Publications: 2 Organism: Homo Sapiens
+ PAK1down-regulates img/direct_inhibition.png phosphorylation PGAM1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-91594 Ser118 QVKIWRRsYDVPPPP Homo sapiens Neutrophil
pmid sentence
Activated pak1 inhibits glycolysis by association of its catalytic domain with pgam-b and subsequent phosphorylation of the enzyme on serine residues 23 and 118, thereby abolishing pgam activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-91598 Ser23 WNLENRFsGWYDADL Homo sapiens Neutrophil
pmid sentence
Activated pak1 inhibits glycolysis by association of its catalytic domain with pgam-b and subsequent phosphorylation of the enzyme on serine residues 23 and 118, thereby abolishing pgam activity.
Publications: 2 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation TBCB 0.458
Identifier Residue Sequence Organism Cell Line
SIGNOR-135460 Ser128 VRSFLKRsKLGRYNE Homo sapiens Breast Cancer Cell
pmid sentence
P21-activated kinase 1 regulates microtubule dynamics by phosphorylating tubulin cofactor b. Pak1 directly phosphorylated tcob in vitro and in vivo on serines 65 and 128 and colocalized with tcob on newly polymerized microtubules and on centrosomes. Pak1 phosphorylation is necessary for normal tcob function
Identifier Residue Sequence Organism Cell Line
SIGNOR-135464 Ser65 GVDDKFYsKLDQEDA Homo sapiens Breast Cancer Cell
pmid sentence
P21-activated kinase 1 regulates microtubule dynamics by phosphorylating tubulin cofactor b. Pak1 directly phosphorylated tcob in vitro and in vivo on serines 65 and 128 and colocalized with tcob on newly polymerized microtubules and on centrosomes. Pak1 phosphorylation is necessary for normal tcob function
Publications: 2 Organism: Homo Sapiens
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation NET1 0.251
Identifier Residue Sequence Organism Cell Line
SIGNOR-263019 Ser152 PTPAKRRsSALWSEM in vitro
pmid sentence
In this work we show that the Rac/Cdc42hs-regulated protein kinase PAK1 down-regulates the activity of the RhoA-specific guanine nucleotide exchange factor NET1. Specifically, PAK1 phosphorylates NET1 on three sites in vitro: serines 152, 153, and 538. Replacement of serines 152 and 153 with glutamate residues down-regulates the activity of NET1 as an exchange factor in vitro and its ability to stimulate actin stress fiber formation in cells. Using a phospho-specific antibody that recognizes NET1 phosphorylated on serine 152, we show that PAK1 phosphorylates NET1 on this site in cells and that Rac1 stimulates serine 152 phosphorylation in a PAK1-dependent manner.
Identifier Residue Sequence Organism Cell Line
SIGNOR-263017 Ser152 PTPAKRRsSALWSEM in vitro
pmid sentence
In this work we show that the Rac/Cdc42hs-regulated protein kinase PAK1 down-regulates the activity of the RhoA-specific guanine nucleotide exchange factor NET1. Specifically, PAK1 phosphorylates NET1 on three sites in vitro: serines 152, 153, and 538. Replacement of serines 152 and 153 with glutamate residues down-regulates the activity of NET1 as an exchange factor in vitro and its ability to stimulate actin stress fiber formation in cells. Using a phospho-specific antibody that recognizes NET1 phosphorylated on serine 152, we show that PAK1 phosphorylates NET1 on this site in cells and that Rac1 stimulates serine 152 phosphorylation in a PAK1-dependent manner.
Identifier Residue Sequence Organism Cell Line
SIGNOR-263018 Ser539 LTAQRRAsTVSSVTQ in vitro
pmid sentence
In this work we show that the Rac/Cdc42hs-regulated protein kinase PAK1 down-regulates the activity of the RhoA-specific guanine nucleotide exchange factor NET1. Specifically, PAK1 phosphorylates NET1 on three sites in vitro: serines 152, 153, and 538. Replacement of serines 152 and 153 with glutamate residues down-regulates the activity of NET1 as an exchange factor in vitro and its ability to stimulate actin stress fiber formation in cells. Using a phospho-specific antibody that recognizes NET1 phosphorylated on serine 152, we show that PAK1 phosphorylates NET1 on this site in cells and that Rac1 stimulates serine 152 phosphorylation in a PAK1-dependent manner.
Publications: 3 Organism: In Vitro
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation CTBP1 0.413
Identifier Residue Sequence Organism Cell Line
SIGNOR-103943 Ser158 REGTRVQsVEQIREV Homo sapiens
pmid sentence
Pak1 phosphorylates ctbp selectively on ser158 within a putative regulatory loop, triggering ctbp cellular redistribution and blocking ctbp ak1 superphosphorylates ctbp and inhibits ctbp dehydrogenase activitycorepressor functions.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates img/direct_inhibition.png phosphorylation STMN1 0.383
Identifier Residue Sequence Organism Cell Line
SIGNOR-119483 Ser16 KELEKRAsGQAFELI Homo sapiens
pmid sentence
Pak1 phosphorylates op18/stathmin specifically at serine 16 and inactivates its catastrophe promoting activity in biochemical and time lapse microscopy microtubule assembly assays. Furthermore, phosphorylation of either serine 16 or 63 is sufficient to inhibit op18/stathmin in vitro.
Identifier Residue Sequence Organism Cell Line
SIGNOR-183503 Ser38 SVPEFPLsPPKKKDL Homo sapiens
pmid sentence
The hgf-induced wave2 transport, lamellipodia formation, stathmin/op18 phosphorylation at ser38 and binding to kinesin-wave2 complex, but not stathmin/op18 phosphorylation at ser25 and microtubule growth, were abrogated by pak1 inhibitor ipa-3
Identifier Residue Sequence Organism Cell Line
SIGNOR-79955 Ser38 SVPEFPLsPPKKKDL Homo sapiens
pmid sentence
We find that, in vitro, pak1 phosphorylates op18/stathmin specifically at serine 16 and inactivates its catastrophe promoting activity in biochemical and time lapse microscopy microtubule assembly assays.
Publications: 3 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation GNAZ 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-48673 Ser16 EKEAARRsRRIDRHL Homo sapiens
pmid sentence
Phosphorylation of either ser(16) by pak1 or ser(27) by pkc decreased the affinity of galpha(z) for gbetagamma;phosphorylation of both residues by pkc caused no further effect. Pak1 thus regulates galpha(z) function by attenuating the inhibitory effects of both gaps and gbetagamma.
Publications: 1 Organism: Homo Sapiens
Tissue: Kidney
+ CDK11Aup-regulates activity img/direct-activation.png phosphorylation PAK1 0.391
Identifier Residue Sequence Organism Cell Line
SIGNOR-273026 Ser174 TPAVPPVsEDEDDDD
pmid sentence
CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression|We show that PAK1 is a substrate of CDK11p58 and can be strongly activated upon phosphorylation.
Publications: 1
+ CyclinD3/CDK11Aup-regulates activity img/direct-activation.png phosphorylation PAK1 0.391
Identifier Residue Sequence Organism Cell Line
SIGNOR-273124 Ser174 TPAVPPVsEDEDDDD
pmid sentence
CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression|We show that PAK1 is a substrate of CDK11p58 and can be strongly activated upon phosphorylation.
Publications: 1
+ CyclinD3/CDK11Bup-regulates activity img/direct-activation.png phosphorylation PAK1 0.394
Identifier Residue Sequence Organism Cell Line
SIGNOR-273129 Ser174 TPAVPPVsEDEDDDD
pmid sentence
CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression|We show that PAK1 is a substrate of CDK11p58 and can be strongly activated upon phosphorylation.
Publications: 1
+ CDK19 img/unknown.png phosphorylation PAK1 0.336
Identifier Residue Sequence Organism Cell Line
SIGNOR-185000 Ser174 TPAVPPVsEDEDDDD Homo sapiens
pmid sentence
Here, we identified p21 activated kinase 1 (pak1) as a new cdk11(p58) substrate and we mapped a new phosphorylation site of ser174 on pak1
Publications: 1 Organism: Homo Sapiens
+ PPM1Adown-regulates activity img/direct_inhibition.png dephosphorylation PAK1 0.341
Identifier Residue Sequence Organism Cell Line
SIGNOR-248492 Ser199 PRPEHTKsVYTRSVI Rattus norvegicus
pmid sentence
Purified PP2Cα protein efficiently dephosphorylated PAK1 in vitro (Fig. 1, D and E). We previously assessed the time course of phospho-PAK1 dephosphorylation assessed using specific antibodies against either Ser(P)198/203 or Thr(P)422 sites in the PAK1 activation loop.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248493 Ser57 KKDRFYRsILPGDKT Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248491 Thr423 PEQSKRStMVGTPYW Rattus norvegicus
pmid sentence
Purified PP2Cα protein efficiently dephosphorylated PAK1 in vitro (Fig. 1, D and E). We previously assessed the time course of phospho-PAK1 dephosphorylation assessed using specific antibodies against either Ser(P)198/203 or Thr(P)422 sites in the PAK1 activation loop.
Publications: 3 Organism: Rattus Norvegicus
+ PPP2CBdown-regulates activity img/direct_inhibition.png dephosphorylation PAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-248599 Ser199 PRPEHTKsVYTRSVI Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248598 Ser57 KKDRFYRsILPGDKT Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248600 Thr423 PEQSKRStMVGTPYW Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Publications: 3 Organism: Rattus Norvegicus
+ PAK1up-regulates activity img/direct-activation.png phosphorylation PAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-250216 Ser199 PRPEHTKsVYTRSVI Chlorocebus aethiops
pmid sentence
Cdc42 and Rac1 cause alpha-PAK autophosphorylation and kinase activation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-250219 Ser57 KKDRFYRsILPGDKT Chlorocebus aethiops COS-7 Cell
pmid sentence
Cdc42 and Rac1 cause alpha-PAK autophosphorylation and kinase activation.
Publications: 2 Organism: Chlorocebus Aethiops
Pathways:EGFR Signaling, T cell activation, Toll like receptors
+ PPP2CAdown-regulates activity img/direct_inhibition.png dephosphorylation PAK1 0.363
Identifier Residue Sequence Organism Cell Line
SIGNOR-248642 Ser199 PRPEHTKsVYTRSVI Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248641 Ser57 KKDRFYRsILPGDKT Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248643 Thr423 PEQSKRStMVGTPYW Rattus norvegicus
pmid sentence
Both sites were dephosphorylated with the same kinetics; the anti-Ser(P)198 antibody was subsequently used as it exhibited lower background staining. Direct comparison of PP2Cα with purified PP1 and PP2A lead us to conclude that at the same molar ratio PP2Cα was the most efficient in dephosphorylating PAK1 (Fig. 1D). In this case we monitored two autophosphorylation sites in the Pak1 N-terminal regulatory region (Ser57 and Ser198/203) using phosphospecific antibodies: both sites showed the same kinetics of inactivation.
Publications: 3 Organism: Rattus Norvegicus
+ PAK1up-regulates img/direct-activation.png phosphorylation ELF3 0.467
Identifier Residue Sequence Organism Cell Line
SIGNOR-154743 Ser207 GTGASRSsHSSDSGG Homo sapiens Breast Cancer Cell
pmid sentence
Phosphorylation-dependent regulation of stability and transforming potential of ets transcriptional factor ese-1 by p21-activated kinase 1. Pak1 selectively phosphorylates ese-1 at ser(207). Intriguingly, pak1 phosphorylation inactive mutant ese1-s207a is more unstable
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation FLNA 0.784
Identifier Residue Sequence Organism Cell Line
SIGNOR-92065 Ser2152 TRRRRAPsVANVGSH Homo sapiens
pmid sentence
In flna, the pak1-binding site involves tandem repeat 23 in the carboxyl terminus and phosphorylation takes place on serine 2152.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation SNAI1 0.41
Identifier Residue Sequence Organism Cell Line
SIGNOR-135605 Ser246 QACARTFsRMSLLHK Homo sapiens Breast Cancer Cell
pmid sentence
Pak1 regulates the repressor activity of snail by phosphorylating on ser(246). Pak1 phosphorylation of snail supports snail's accumulation in the nucleus as well as its repressor functions.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation ILK 0.42
Identifier Residue Sequence Organism Cell Line
SIGNOR-154303 Ser246 CPRLRIFsHPNVLPV Homo sapiens
pmid sentence
We found that pak1 phosphorylates ilk at threonine-173 and serine-246 in vitro and in vivo. together, these results suggest that ilk is a pak1 substrate, undergoes phosphorylation-dependent shuttling between the cell nucleus and cytoplasm, and interacts with gene-regulatory chromatin.
Identifier Residue Sequence Organism Cell Line
SIGNOR-154307 Thr173 DTFWKGTtRTRPRNG Homo sapiens
pmid sentence
We found that pak1 phosphorylates ilk at threonine-173 and serine-246 in vitro and in vivo. together, these results suggest that ilk is a pak1 substrate, undergoes phosphorylation-dependent shuttling between the cell nucleus and cytoplasm, and interacts with gene-regulatory chromatin.
Publications: 2 Organism: Homo Sapiens
+ PAK1 img/unknown.png phosphorylation PXN 0.653
Identifier Residue Sequence Organism Cell Line
SIGNOR-146842 Ser272 ELDELMAsLSDFKIQ Homo sapiens
pmid sentence
We show that p21-activated kinase (pak)-induced phosphorylation of serine 273 in paxillin is a critical regulator of this turnover.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation ITGB3BP 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-178795 Ser28 SKITRKKsVITYSPT Homo sapiens Breast Cancer Cell
pmid sentence
Serine 28 phosphorylation of nrif3 confers its co-activator function for estrogen receptor-alpha transactivation. p21-activated protein kinase 1 (pak1) phosphorylates eralpha at ser305 and this modification is important in eralpha transactivation function.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates activity img/direct-activation.png phosphorylation MAP2K1 0.567
Identifier Residue Sequence Organism Cell Line
SIGNOR-236002 Ser298 RTPGRPLsSYGMDSR Homo sapiens REF-52 Cell
pmid sentence
We find that adhesion to fibronectin induces pak1-dependent phosphorylation of mek1 on s298 and that this phosphorylation is necessary for efficient activation of mek1 and subsequent mapk activation.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation ARAF 0.269
Identifier Residue Sequence Organism Cell Line
SIGNOR-236342 Ser299 KNLGYRDsGYYWEVP Homo sapiens Melanoma Cell
pmid sentence
Phosphorylation of endogenous a-raf, b-raf and raf-1 on the homologous pak phosphorylation sites (serine 299, serine 445, or serine 338 respectively)we found that the phosphorylation of a-raf on serine 299 was also stimulated by egf, although the duration of phosphorylation on this site was much shorter than for raf-1. Thus, by analogy with raf-1, phosphorylation of this site may play an important role in the a-raf activation mechanism.
Publications: 1 Organism: Homo Sapiens
Pathways:EGFR Signaling
+ PAK1up-regulates img/direct-activation.png phosphorylation ESR1 0.548
Identifier Residue Sequence Organism Cell Line
SIGNOR-94206 Ser305 IKRSKKNsLALSLTA Homo sapiens
pmid sentence
Pak1 directly phosphorylated the activation function-2 domain of the er at the n-terminal residue ser305, and its mutation to ala (s305a) abolished the pak1-mediated phosphorylation and transactivation functions of the er
Publications: 1 Organism: Homo Sapiens
Tissue: Breast
+ PAK1up-regulates img/direct-activation.png phosphorylation RAF1 0.587
Identifier Residue Sequence Organism Cell Line
SIGNOR-180808 Ser338 RPRGQRDsSYYWEIE Homo sapiens
pmid sentence
P21-activated protein kinases (paks) are serine/threonine protein kinases that phosphorylate raf-1 at ser-338 and ser-339.
Identifier Residue Sequence Organism Cell Line
SIGNOR-180812 Ser339 PRGQRDSsYYWEIEA Homo sapiens
pmid sentence
P21-activated protein kinases (paks) are serine/threonine protein kinases that phosphorylate raf-1 at ser-338 and ser-339.
Publications: 2 Organism: Homo Sapiens
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation HACE1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-255537 Ser385 LMKNKRDsTEITSIL Homo sapiens Uveal Melanoma Cell
pmid sentence
Using a proteomic approach, we identified serine 385 as a target of group-I PAK kinases […] We have established in vitro that HACE1 is a direct target of PAK1 kinase activity.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation CTTN 0.698
Identifier Residue Sequence Organism Cell Line
SIGNOR-165216 Ser405 KTQTPPVsPAPQPTE Homo sapiens
pmid sentence
Strikingly, we find that pak1 phosphorylation of cortactin on serine residues 405 and 418 increases its association with n-wasp. Thus, pak1, by controlling the interaction between cortactin and n-wasp, could regulate the polymerization of actin during clathrin-independent endocytosis.
Identifier Residue Sequence Organism Cell Line
SIGNOR-169690 Ser405 KTQTPPVsPAPQPTE Homo sapiens
pmid sentence
Strikingly, we find that pak1 phosphorylation of cortactin on serine residues 405 and 418 increases its association with n-wasp. Thus, pak1, by controlling the interaction between cortactin and n-wasp, could regulate the polymerization of actin during clathrin-independent endocytosis.
Identifier Residue Sequence Organism Cell Line
SIGNOR-169694 Ser418 TEERLPSsPVYEDAA Homo sapiens
pmid sentence
Strikingly, we find that pak1 phosphorylation of cortactin on serine residues 405 and 418 increases its association with n-wasp. Thus, pak1, by controlling the interaction between cortactin and n-wasp, could regulate the polymerization of actin during clathrin-independent endocytosis.
Identifier Residue Sequence Organism Cell Line
SIGNOR-165220 Ser418 TEERLPSsPVYEDAA Homo sapiens
pmid sentence
Strikingly, we find that pak1 phosphorylation of cortactin on serine residues 405 and 418 increases its association with n-wasp. Thus, pak1, by controlling the interaction between cortactin and n-wasp, could regulate the polymerization of actin during clathrin-independent endocytosis.
Publications: 4 Organism: Homo Sapiens
Pathways:T cell activation
+ PAK1up-regulates activity img/direct-activation.png phosphorylation FXR1 0.273
Identifier Residue Sequence Organism Cell Line
SIGNOR-273713 Ser420 SERKDELsDWSLAGE Chlorocebus aethiops COS-7 Cell
pmid sentence
Identification of Ser420 in FXR1 as a PAK1 Kinase Target. During zebrafish muscle development, FXR1 Ser420 phosphorylation is needed for protein function.
Publications: 1 Organism: Chlorocebus Aethiops
+ PAK1up-regulates img/direct-activation.png phosphorylation PLK1 0.535
Identifier Residue Sequence Organism Cell Line
SIGNOR-178353 Ser49 EVLVDPRsRRRYVRG Homo sapiens
pmid sentence
We show here that pak1 is required for cell proliferation, mitotic progression and plk1 activity in hela cells. phosphorylation of plk1 on ser 49 is important for metaphase-associated events.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates img/direct_inhibition.png phosphorylation NF2 0.633
Identifier Residue Sequence Organism Cell Line
SIGNOR-159764 Ser518 DTDMKRLsMEIEKEK Homo sapiens
pmid sentence
Merlin contains a c-terminal serine 518, which is phosphorylated both by p21-activated kinase (pak) and protein kinase a (pka) (shaw et al., 2001;kissil et al., 2002;xiao et al., 2002;alfthan et al., 2004). Phosphorylation at this site is predicted to result in a more open conformation incapable of inhibiting cell growth,
Publications: 1 Organism: Homo Sapiens
+ PAK1 img/unknown.png phosphorylation VIM 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-134520 Ser56 SRSLYASsPGGVYAT Homo sapiens
pmid sentence
In the present study, pak1 was able to phosphorylate vimentin on ser-56 in vitro.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle, Smooth Muscle
+ PPM1Edown-regulates activity img/direct_inhibition.png dephosphorylation PAK1 0.307
Identifier Residue Sequence Organism Cell Line
SIGNOR-248760 Ser57 KKDRFYRsILPGDKT Rattus norvegicus
pmid sentence
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family|POPX Can Dephosphorylate and Downregulate PAK| To confirm that POPX2 acts on αPAK phospho-Thr422, a key regulator of activity in the kinase activation loop [9], we used phospho-specific antibodies against αPAK P-Thr422 (Figure 3B, lower panel), which proved to be an excellent substrate for POPX2. Similarly, complete loss of αPAK P-Ser57 with 0.2 μg POPX2 contrasts with the slight loss observed with 1.5 μg PP1. On the basis of these results, we suggest PAK is a substrate of POPX.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248761 Thr423 PEQSKRStMVGTPYW Rattus norvegicus
pmid sentence
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family|POPX Can Dephosphorylate and Downregulate PAK| To confirm that POPX2 acts on αPAK phospho-Thr422, a key regulator of activity in the kinase activation loop [9], we used phospho-specific antibodies against αPAK P-Thr422 (Figure 3B, lower panel), which proved to be an excellent substrate for POPX2. Similarly, complete loss of αPAK P-Ser57 with 0.2 μg POPX2 contrasts with the slight loss observed with 1.5 μg PP1. On the basis of these results, we suggest PAK is a substrate of POPX.
Publications: 2 Organism: Rattus Norvegicus
+ PPM1Fdown-regulates activity img/direct_inhibition.png dephosphorylation PAK1 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-248530 Ser57 KKDRFYRsILPGDKT Rattus norvegicus
pmid sentence
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family|POPX Can Dephosphorylate and Downregulate PAK| To confirm that POPX2 acts on αPAK phospho-Thr422, a key regulator of activity in the kinase activation loop [9], we used phospho-specific antibodies against αPAK P-Thr422 (Figure 3B, lower panel), which proved to be an excellent substrate for POPX2. Similarly, complete loss of αPAK P-Ser57 with 0.2 μg POPX2 contrasts with the slight loss observed with 1.5 μg PP1. On the basis of these results, we suggest PAK is a substrate of POPX.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248531 Thr423 PEQSKRStMVGTPYW Rattus norvegicus
pmid sentence
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family|POPX Can Dephosphorylate and Downregulate PAK| To confirm that POPX2 acts on αPAK phospho-Thr422, a key regulator of activity in the kinase activation loop [9], we used phospho-specific antibodies against αPAK P-Thr422 (Figure 3B, lower panel), which proved to be an excellent substrate for POPX2. Similarly, complete loss of αPAK P-Ser57 with 0.2 μg POPX2 contrasts with the slight loss observed with 1.5 μg PP1. On the basis of these results, we suggest PAK is a substrate of POPX.
Publications: 2 Organism: Rattus Norvegicus
+ PAK1up-regulates activity img/direct-activation.png phosphorylation SYN1 0.354
Identifier Residue Sequence Organism Cell Line
SIGNOR-250235 Ser605 AGPTRQAsQAGPVPR Rattus norvegicus PC-12 Cell
pmid sentence
Synapsin I is phosphorylated at Ser603 by p21-activated kinases. the Ser603 residue must be one of the pivotal sites for the release
Publications: 1 Organism: Rattus Norvegicus
+ PAK1up-regulates activity img/direct-activation.png phosphorylation MAP3K1 0.521
Identifier Residue Sequence Organism Cell Line
SIGNOR-236006 Ser67 RQLRKVRsVELDQLP Homo sapiens HEK-293 Cell
pmid sentence
We found that pak1 phosphorylated mekk1 on serine 67 of its amino-terminal regulatory domain. mekk1 activity was increased modestly following pak phosphorylation.
Publications: 1 Organism: Homo Sapiens
Pathways:EGFR Signaling, Toll like receptors
+ PAK1up-regulates img/direct-activation.png phosphorylation CTNNB1 0.544
Identifier Residue Sequence Organism Cell Line
SIGNOR-175944 Ser675 QDYKKRLsVELTSSL Homo sapiens
pmid sentence
Pak1 directly phosphorylates _-catenin proteins at ser675 site and this leads to more stable and transcriptional active _-catenin
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates activity img/direct-activation.png phosphorylation MORC2 0.374
Identifier Residue Sequence Organism Cell Line
SIGNOR-273714 Ser739 ATPSRKRsVAVSDEE Homo sapiens BGC-823 Cell
pmid sentence
We demonstrate that PAK1-mediated MORC2 phosphorylation promotes cell cycle progression, defective phosphorylation of MORC2-S677A results in attenuated cell proliferation and tumorigenicity of gastric cancer cells, which is significantly enhanced in overexpression of phospho-mimic MORC2-S677E form, suggesting the importance of MORC2 phosphorylation in tumorigenesis. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-278414 Ser739 ATPSRKRsVAVSDEE Homo sapiens
pmid sentence
In support of this notion, selective knockdown of endogenous PAK1 significantly reduced the association of MORC2 with chromatin (XREF_FIG, compare lane 4 with 3).|PAK1 phosphorylation of MORC2 on serine 739 regulates an ATPase-dependent chromatin remodeling following DSB damage and facilitates efficient DSB repair.
Publications: 2 Organism: Homo Sapiens
+ PAK1down-regulates img/direct_inhibition.png phosphorylation BAD 0.339
Identifier Residue Sequence Organism Cell Line
SIGNOR-177271 Ser74 VEIRSRHsSYPAGTE Homo sapiens
pmid sentence
Bad is a pro-apoptotic member of the bcl-2 family of proteins, which can be phosphorylated on numerous sites to modulate binding to bcl-2 and 14-3-3 proteins and inhibit its pro-apoptotic activities. Together, these findings demonstrate that pak1 phosphorylates bad directly at s111, but phosphorylated s112 through raf-1. These two sites of bad serve as redundant regulatory sites for bcl-2 binding
Identifier Residue Sequence Organism Cell Line
SIGNOR-73529 Ser75 EIRSRHSsYPAGTED Homo sapiens
pmid sentence
Pak phosphorylates bad in vitro and in vivo on ser112 and ser136, resulting in a markedly reduced interaction between bad and bcl-2 or bcl-x(l) and the increased association of bad with 14-3-3tau.
Identifier Residue Sequence Organism Cell Line
SIGNOR-73533 Ser99 PFRGRSRsAPPNLWA Homo sapiens
pmid sentence
Pak phosphorylates bad in vitro and in vivo on ser112 and ser136, resulting in a markedly reduced interaction between bad and bcl-2 or bcl-x(l) and the increased association of bad with 14-3-3tau.
Publications: 3 Organism: Homo Sapiens
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation SORT1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273718 Ser793 RFLVHRYsVLQQHAE Homo sapiens HEK-293 Cell
pmid sentence
PAKs specifically phosphorylate Ser15 of the sortilin-cd and alter its trafficking. It can be concluded that PAK1-3 may indeed instigate the phosphorylation of sortilin and that they target a single serine residue (Ser15) located in the kinase domain-binding site of the sortilin-cd. Full-length sortilins with the serine at position 793 (residue 15 in the cytoplasmic domain) (for the sequence, see Fig. 2). Phosphorylation (Ser15) downregulates the sortilin–AP-1 interaction.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates img/direct_inhibition.png phosphorylation DYNLL1 0.384
Identifier Residue Sequence Organism Cell Line
SIGNOR-159995 Ser88 VAILLFKsG Homo sapiens
pmid sentence
Dlc1 phosphorylation on ser(88) by p21-activated kinase 1 (pak1), a signaling nodule, promotes mammalian cell survival by regulating its interaction with bim and the stability of bim. Here we discovered that phosphorylation of ser(88), which juxtapose each other at the interface of the dlc dimer, disrupts dlc1 dimer formation and consequently impairs its interaction with bim
Publications: 1 Organism: Homo Sapiens
Tissue: Breast
+ PAK1down-regulates img/direct_inhibition.png phosphorylation ARHGEF2 0.358
Identifier Residue Sequence Organism Cell Line
SIGNOR-187573 Ser886 PVDPRRRsLPAGDAL Homo sapiens
pmid sentence
We identify gef-h1 as a binding target and substrate for p21-activated kinase 1 (pak1), we show that phosphorylation of gef-h1 at ser(885) by pak1 induces 14-3-3 binding to the exchange factor and relocation of 14-3-3 to microtubules.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates quantity by stabilization img/direct-activation.png phosphorylation ATG5 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275835 Thr101 SALPWNItVHFKSFP
pmid sentence
Here, we identified USP13 as an essential deubiquitinase that stabilizes ATG5 in a process that depends on the PAK1 serine/threonine-protein kinase and which enhances autophagy and promotes IM resistance in GIST cells. |As PAK1-mediated phosphorylation at residue T101 protects ATG5 from ubiquitination-dependent degradation
Publications: 1
+ STK11down-regulates img/direct_inhibition.png phosphorylation PAK1 0.248
Identifier Residue Sequence Organism Cell Line
SIGNOR-164814 Thr109 QWARLLQtSNITKSE Homo sapiens
pmid sentence
Lkb1 suppresses p21-activated kinase-1 (pak1) by phosphorylation of thr109 in the p21-binding domain.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation ARPC1B 0.545
Identifier Residue Sequence Organism Cell Line
SIGNOR-121642 Thr21 HAWNKDRtQIAICPN Homo sapiens
pmid sentence
The formation of new branched actin filament networks at the cell cortex of migrating cells is choreographed by the actin-related protein (arp) 2/3 complex. Despite the fundamental role of the arp2/3 complex in actin nucleation and branching, upstream signals that control the functions of p41-arc, a putative regulatory component of the mammalian arp2/3 complex. Pak1 phosphorylation of p41-arc regulates its localization with the arp2/3 complex in the cortical nucleation regions of cells. Pak1 phosphorylates p41-arc on threonine 21
Publications: 1 Organism: Homo Sapiens
+ CDK5down-regulates activity img/direct_inhibition.png phosphorylation PAK1 0.523
Identifier Residue Sequence Organism Cell Line
SIGNOR-249328 Thr212 VIEPLPVtPTRDVAT Chlorocebus aethiops COS Cell
pmid sentence
Our previous work revealed that the neuronal p35/Cdk5 kinase associates with Pak1 in a RacGTP-dependent manner, causing hyperphosphorylation and down-regulation of Pak1 kinase activity. We have now demonstrated direct phosphorylation of Pak1 on threonine 212 by the p35/Cdk5 kinase.
Publications: 1 Organism: Chlorocebus Aethiops
+ MAPK1down-regulates activity img/direct_inhibition.png phosphorylation PAK1 0.409
Identifier Residue Sequence Organism Cell Line
SIGNOR-249432 Thr212 VIEPLPVtPTRDVAT Rattus norvegicus Smooth Muscle
pmid sentence
We also show that ERK2 phosphorylates PAK1 on Thr(212) in vitro and that Thr(212) is phosphorylated in smooth muscle cells following PDGF-BB treatment in an adhesion- and MEK/ERK-dependent fashion. Expression of a phosphomimic variant, PAK-T212E, does not alter ERK association, but markedly attenuates downstream ERK signaling. Taken together, these data suggest that PAK1 may facilitate ERK signaling by serving as a scaffold to recruit Raf, MEK, and ERK to adhesion complexes, and that subsequent growth factor-stimulated phosphorylation of PAK-Thr(212) by ERK may serve to provide a negative feedback signal
Publications: 1 Organism: Rattus Norvegicus
+ PAK1up-regulates img/direct-activation.png phosphorylation PA2G4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-160963 Thr261 QYGLKMKtSRAFFSE Homo sapiens Breast Cancer Cell
pmid sentence
We found that pak1 phosphorylated ebp1 in vitro and mapped the phosphorylation site to threonine 261. these studies demonstrate for the first time that ebp1 is a substrate of pak1 and the importance of the pak1 phosphorylation site for the functional activity of ebp1 in breast cancer cells.
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates img/direct-activation.png phosphorylation AURKA 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-205110 Thr288 APSSRRTtLCGTLDY Homo sapiens
pmid sentence
The upstream pak1 kinase can phosphorylate aurora a at t288, autophosphorylation appears to be the essential mode of activation. Our experiments suggest that phosphorylation of t288 is important for regulation of the aurora2 kinase both for its activity and its stability
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation PAK1 0.342
Identifier Residue Sequence Organism Cell Line
SIGNOR-123074 Thr292 YTAMDVAtGQEVAIK Homo sapiens
pmid sentence
Activated erk can phosphorylate t292 in the prs, and this blocks the ability of pak to phosphorylate s298 and of rac-pak signaling to enhance mek1-erk complex formation.
Publications: 1 Organism: Homo Sapiens
+ PDPK1up-regulates activity img/direct-activation.png phosphorylation PAK1 0.362
Identifier Residue Sequence Organism Cell Line
SIGNOR-250267 Thr423 PEQSKRStMVGTPYW Chlorocebus aethiops COS-7 Cell
pmid sentence
P21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1). We identify threonine 423, a conserved threonine in the activation loop of kinase subdomain VIII, as the PDK1 phosphorylation site on PAK1.
Publications: 1 Organism: Chlorocebus Aethiops
Pathways:EGFR Signaling, T cell activation
+ PAK1up-regulates img/direct-activation.png phosphorylation PGM1 0.348
Identifier Residue Sequence Organism Cell Line
SIGNOR-127135 Thr467 SANDKVYtVEKADNF Homo sapiens Leukemia Cell
pmid sentence
The signaling kinase p21-activated kinase 1 (pak1) binds to, phosphorylates and enhances the enzymatic activity of phosphoglucomutase 1 (pgm),
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates activity img/direct-activation.png phosphorylation LIMK1 0.6
Identifier Residue Sequence Organism Cell Line
SIGNOR-72142 Thr508 PDRKKRYtVVGNPYW Homo sapiens
pmid sentence
Activation of lim-kinase by pak1 couplesp21-activated kinase (pak1) phosphorylates lim-kinase at threonine residue 508 within lim-kinase's activation loop
Publications: 1 Organism: Homo Sapiens
+ PAK1up-regulates activity img/direct-activation.png phosphorylation NLRP3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277547 Thr659 KIEINLStRMDHMVS Homo sapiens HEK-293T Cell
pmid sentence
Pak1 phosphorylates NLRP3 to promote inflammasome activation.
Publications: 1 Organism: Homo Sapiens
+ OXSR1down-regulates activity img/direct_inhibition.png phosphorylation PAK1 0.388
Identifier Residue Sequence Organism Cell Line
SIGNOR-250210 Thr84 LPSDFEHtIHVGFDA Homo sapiens HeLa Cell
pmid sentence
OSR1 phosphorylated threonine 84 in the N-terminal regulatory domain of PAK1. phosphorylation of PAK1 by OSR1 desensitizes PAK1 to activation by small G proteins, providing a modulatory input to PAK1 activity.
Publications: 1 Organism: Homo Sapiens
+ EPHA3up-regulates activity img/direct-activation.png phosphorylation PAK1
Identifier Residue Sequence Organism Cell Line
SIGNOR-278398 Tyr153 TDKSAEDyNSSNALN Homo sapiens
pmid sentence
Etk kinase directly phosphorylates and activates PAK1 in response to estrogen.|We demonstrated that estrogen-activated Etk directly phosphorylated PAK1 on Tyr153.
Publications: 1 Organism: Homo Sapiens
+ RAC2up-regulates img/direct-activation.png binding PAK1 0.774
Identifier Residue Sequence Organism Cell Line
SIGNOR-59546 Homo sapiens
pmid sentence
This report shows that rac1 binds to and stimulates the kinase activity of pak1 approximately 2- and 4-5-fold, respectively, better than rac2.
Publications: 1 Organism: Homo Sapiens
+ Gbetadown-regulates img/direct_inhibition.png phosphorylation PAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-270086 Homo sapiens
pmid sentence
Activated erk can phosphorylate t292 in the prs, and this blocks the ability of pak to phosphorylate s298 and of rac-pak signaling to enhance mek1-erk complex formation.
Publications: 1 Organism: Homo Sapiens
+ CDC42up-regulates activity img/direct-activation.png binding PAK1 0.941
Identifier Residue Sequence Organism Cell Line
SIGNOR-248243 Mus musculus Brain
pmid sentence
A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways.
Publications: 1 Organism: Mus Musculus
+ PAK1down-regulates img/direct_inhibition.png phosphorylation WEE1 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-123528 Homo sapiens
pmid sentence
Kinases targeted sequentially to the neck, cla4/pak and cdc5/polo, are responsible for stepwise phosphorylation and down-regulation of swe1.
Publications: 1 Organism: Homo Sapiens
+ ERK1/2down-regulates img/direct_inhibition.png phosphorylation PAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-270186 Homo sapiens
pmid sentence
Activated erk can phosphorylate t292 in the prs, and this blocks the ability of pak to phosphorylate s298 and of rac-pak signaling to enhance mek1-erk complex formation.
Publications: 1 Organism: Homo Sapiens
Pathways:EGFR Signaling, T cell activation, Toll like receptors
+ RAC1up-regulates activity img/direct-activation.png binding PAK1 0.783
Identifier Residue Sequence Organism Cell Line
SIGNOR-248236 Mus musculus Brain
pmid sentence
A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways.
Publications: 1 Organism: Mus Musculus
+ PAK1down-regulates activity img/direct_inhibition.png binding PAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-236338 Homo sapiens HEK-293 Cell
pmid sentence
We show that pak1 forms homodimers in vivo and that its dimerization is regulated by the intracellular level of gtp-cdc42 or gtp-rac1. The dimerized pak1 adopts a trans-inhibited conformation.
Publications: 1 Organism: Homo Sapiens
Pathways:EGFR Signaling, T cell activation, Toll like receptors
+ PPM1Fdown-regulates img/direct_inhibition.png dephosphorylation PAK1 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-162146 Homo sapiens
pmid sentence
Pop x2, a pp 2c serine/threonine phosphatase, is known to dephosphorylate pak and downregulate its activity.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation BCL6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-253930 Homo sapiens
pmid sentence
The transcriptional repressor B-cell lymphoma (BCL)-6 downregulates genes involved in cell-cycle progression and becomes inactivated following phosphorylation by the Rac1 GTPase-activated protein kinase PAK1.
Publications: 1 Organism: Homo Sapiens
+ NCK1up-regulates img/direct-activation.png binding PAK1 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-236324 Chlorocebus aethiops
pmid sentence
We describe here a specific interaction of the Nck adapter molecule with PAK1 both in vitro and in vivo. Association of Nck with PAK1 may serve to link this important regulatory kinase to cell activation by growth factor receptors.
Identifier Residue Sequence Organism Cell Line
SIGNOR-235947 Homo sapiens
pmid sentence
Both nck and grb4 proteins could associate with receptor tyrosine kinases and the sh3-binding proteins pak, sos1, and prk2, and they synergized with v-abl and sos to induce gene expression via the transcription factor elk-1. Association of nck with pak1 may serve to link this important regulatory kinase to cell activation by growth factor receptors.
Publications: 2 Organism: Chlorocebus Aethiops, Homo Sapiens
Pathways:EGFR Signaling, T cell activation
+ PAK1up-regulates activity img/direct-activation.png phosphorylation MEK1/2 0.567
Identifier Residue Sequence Organism Cell Line
SIGNOR-244924 Homo sapiens
pmid sentence
We find that adhesion to fibronectin induces pak1-dependent phosphorylation of mek1 on s298 and that this phosphorylation is necessary for efficient activation of mek1 and subsequent mapk activation.
Publications: 1 Organism: Homo Sapiens
Pathways:EGFR Signaling, T cell activation, Toll like receptors
+ PAK1down-regulates img/direct_inhibition.png phosphorylation PGAM 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-270283 Homo sapiens Neutrophil
pmid sentence
Activated pak1 inhibits glycolysis by association of its catalytic domain with pgam-b and subsequent phosphorylation of the enzyme on serine residues 23 and 118, thereby abolishing pgam activity.
Publications: 1 Organism: Homo Sapiens
+ PAK1down-regulates activity img/direct_inhibition.png phosphorylation MYLK 0.54
Identifier Residue Sequence Organism Cell Line
SIGNOR-250317 Homo sapiens HeLa Cell
pmid sentence
P21-activated kinase 1 (PAK1) phosphorylates MLCK, resulting in decreased MLCK activity. 
Publications: 1 Organism: Homo Sapiens
+ PAK1 img/unknown.png phosphorylation Histone H3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-265363 Homo sapiens
pmid sentence
Histone h3 is a substrate of pak1 both in vitro and in vivo, and it specifically interacted with pak1 but not pak2 or pak3. Site-directed mutagenesis indicated that pak1 phosphorylates histone h3 on ser10.
Publications: 1 Organism: Homo Sapiens
+ CRIPAKup-regulates img/direct-activation.png binding PAK1 0.423
Identifier Residue Sequence Organism Cell Line
SIGNOR-141467 Homo sapiens Breast Cancer Cell
pmid sentence
We further found that cripak interacted with pak1 through the n-terminal regulatory domain and inhibited pak1 kinase in both in vitro and in vivo assays.
Publications: 1 Organism: Homo Sapiens
Tissue: Breast
+ PAK1up-regulates activity img/direct-activation.png phosphorylation AJUBA
Identifier Residue Sequence Organism Cell Line
SIGNOR-278449 Homo sapiens
pmid sentence
The Rac effector PAK1 was also transiently activated upon cell-cell adhesion and directly phosphorylated Ajuba (Thr172).
Publications: 1 Organism: Homo Sapiens
+ NCK1up-regulates activity img/direct-activation.png binding PAK1 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-236512 Mus musculus MEF Cell
pmid sentence
Both nck and grb4 proteins could associate with receptor tyrosine kinases and the sh3-binding proteins pak, sos1, and prk2, and they synergized with v-abl and sos to induce gene expression via the transcription factor elk-1. Association of nck with pak1 may serve to link this important regulatory kinase to cell activation by growth factor receptors.
Publications: 1 Organism: Mus Musculus
Pathways:EGFR Signaling, T cell activation
+ PAK1up-regulates img/indirect-activation.png F-actin_assembly 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-277768 Homo sapiens Prostate Cancer Cell Line
pmid sentence
 RAC1 activation induces the actin remodeling and membrane ruffling necessary to form macropinosomes by activating PAK kinases
Publications: 1 Organism: Homo Sapiens
Pathways:T cell activation
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