| + |
PRKACA | down-regulates activity 
phosphorylation
|
RHOA |
0.518 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250047 |
Ser188 |
ARRGKKKsGCLVL |
Mus musculus |
|
| pmid |
sentence |
| 12654918 |
PKA phosphorylates RhoA on Ser188. the addition of a negative charge to Ser188 is sufficient to diminish both RhoA activation and activity within the context of a cell. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
PRKCE | up-regulates activity 
phosphorylation
|
RHOA |
0.439 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279477 |
Ser188 |
ARRGKKKsGCLVL |
Homo sapiens |
|
| pmid |
sentence |
| 24191200 |
Our laboratory reported that PKCepsilon modulates RhoA activity in HNSCC presumably through posttranslation phosphorylation .|Phosphopeptide mapping revealed that PKCepsilon phosphorylates RhoA at T127 and S188. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279478 |
Thr127 |
DLRNDEHtRRELAKM |
Homo sapiens |
|
| pmid |
sentence |
| 24191200 |
Our laboratory reported that PKCepsilon modulates RhoA activity in HNSCC presumably through posttranslation phosphorylation .|Phosphopeptide mapping revealed that PKCepsilon phosphorylates RhoA at T127 and S188. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
ERK1/2 | up-regulates activity
phosphorylation
|
RHOA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277202 |
Ser88 |
LMCFSIDsPDSLENI |
Chlorocebus aethiops |
COS-7 Cell |
| pmid |
sentence |
| 26816343 |
We have recently reported that Rac1 is phosphorylated on threonine 108 (108T) by extracellular signal-regulated kinases (ERK) in response to epidermal growth factor (EGF) stimulation. Here, we provide evidence that RhoA is phosphorylated by ERK on 88S and 100T in response to EGF stimulation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277203 |
Thr100 |
ENIPEKWtPEVKHFC |
Chlorocebus aethiops |
COS-7 Cell |
| pmid |
sentence |
| 26816343 |
We have recently reported that Rac1 is phosphorylated on threonine 108 (108T) by extracellular signal-regulated kinases (ERK) in response to epidermal growth factor (EGF) stimulation. Here, we provide evidence that RhoA is phosphorylated by ERK on 88S and 100T in response to EGF stimulation. |
|
| Publications: |
2 |
Organism: |
Chlorocebus Aethiops |
| Pathways: | WNT/FLT3 |
| + |
RHOA | up-regulates activity
phosphorylation
|
MSN |
0.611 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268431 |
Thr558 |
LGRDKYKtLRQIRQG |
Homo sapiens |
Blood Platelet |
| pmid |
sentence |
| 35267019 |
Rev-erbα interacted with OPHN-1, promoted RhoA activity and phosphorylation of ERM. etection of phosphorylated ezrin (Thr567)/radixin (Thr564)/moesin (Thr558)(p-ERM) in Rev-erbαfl/flCre− and Rev-erbαfl/flPF4Cre+ platelets using phospho-specific antibodies. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
phosphorylation
|
RDX |
0.47 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268430 |
Thr564 |
AGRDKYKtLRQIRQG |
Homo sapiens |
Blood Platelet |
| pmid |
sentence |
| 35267019 |
Rev-erbα interacted with OPHN-1, promoted RhoA activity and phosphorylation of ERM. etection of phosphorylated ezrin (Thr567)/radixin (Thr564)/moesin (Thr558)(p-ERM) in Rev-erbαfl/flCre− and Rev-erbαfl/flPF4Cre+ platelets using phospho-specific antibodies. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
phosphorylation
|
EZR |
0.76 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268429 |
Thr567 |
QGRDKYKtLRQIRQG |
Homo sapiens |
Blood Platelet |
| pmid |
sentence |
| 35267019 |
Rev-erbα interacted with OPHN-1, promoted RhoA activity and phosphorylation of ERM. etection of phosphorylated ezrin (Thr567)/radixin (Thr564)/moesin (Thr558)(p-ERM) in Rev-erbαfl/flCre− and Rev-erbαfl/flPF4Cre+ platelets using phospho-specific antibodies. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BCR-ABL | down-regulates activity
phosphorylation
|
RHOA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271700 |
Tyr34 |
KDQFPEVyVPTVFEN |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 23027962 |
When these RhoA mutants were coexpressed with Bcr-Abl, phosphorylation levels of Y34F and Y66F RhoA mutants dramatically decreased to 32% and 17%, respectively. As expected, when Y34 and Y66 were both mutated to phenylalanine, phosphorylation was completely abolished. Together, these observations indicate that Y34 and Y66 are the two predominant phosphorylation sites, and that the Src kinase and Bcr-Abl are the two candidate kinases that may phosphorylate these sites.|In contrast to active RhoA, RhoAQ63L(Y34,66E) had a dramatic decrease in RBD binding. This binding fraction was even lower than that of WT RhoA, suggesting phosphorylation at these sites could have a negative effect on RhoA activity |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271699 |
Tyr66 |
DTAGQEDyDRLRPLS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 23027962 |
When these RhoA mutants were coexpressed with Bcr-Abl, phosphorylation levels of Y34F and Y66F RhoA mutants dramatically decreased to 32% and 17%, respectively. As expected, when Y34 and Y66 were both mutated to phenylalanine, phosphorylation was completely abolished. Together, these observations indicate that Y34 and Y66 are the two predominant phosphorylation sites, and that the Src kinase and Bcr-Abl are the two candidate kinases that may phosphorylate these sites.|In contrast to active RhoA, RhoAQ63L(Y34,66E) had a dramatic decrease in RBD binding. This binding fraction was even lower than that of WT RhoA, suggesting phosphorylation at these sites could have a negative effect on RhoA activity |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SRC | down-regulates activity
phosphorylation
|
RHOA |
0.668 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271702 |
Tyr34 |
KDQFPEVyVPTVFEN |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 23027962 |
When these RhoA mutants were coexpressed with Bcr-Abl, phosphorylation levels of Y34F and Y66F RhoA mutants dramatically decreased to 32% and 17%, respectively. As expected, when Y34 and Y66 were both mutated to phenylalanine, phosphorylation was completely abolished. Together, these observations indicate that Y34 and Y66 are the two predominant phosphorylation sites, and that the Src kinase and Bcr-Abl are the two candidate kinases that may phosphorylate these sites.|In contrast to active RhoA, RhoAQ63L(Y34,66E) had a dramatic decrease in RBD binding. This binding fraction was even lower than that of WT RhoA, suggesting phosphorylation at these sites could have a negative effect on RhoA activity |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271701 |
Tyr66 |
DTAGQEDyDRLRPLS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 23027962 |
When these RhoA mutants were coexpressed with Bcr-Abl, phosphorylation levels of Y34F and Y66F RhoA mutants dramatically decreased to 32% and 17%, respectively. As expected, when Y34 and Y66 were both mutated to phenylalanine, phosphorylation was completely abolished. Together, these observations indicate that Y34 and Y66 are the two predominant phosphorylation sites, and that the Src kinase and Bcr-Abl are the two candidate kinases that may phosphorylate these sites.|In contrast to active RhoA, RhoAQ63L(Y34,66E) had a dramatic decrease in RBD binding. This binding fraction was even lower than that of WT RhoA, suggesting phosphorylation at these sites could have a negative effect on RhoA activity |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
GNA12 | up-regulates
binding
|
RHOA |
0.558 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-192108 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23450633 |
Ga12/13 recruitment of rho-gefs causes rhoa activation and f-actin assembly, which promotes lats1/lat2 inactivation by an unknown, but myosin-independent mechanism. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MYO9B | down-regulates activity
gtpase-activating protein
|
RHOA |
0.774 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260509 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
binding
|
PKN1 |
0.852 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275465 |
|
|
Mus musculus |
Bone Marrow-derived Macrophage |
| pmid |
sentence |
| 27270401 |
PKNs bind to human pyrin and phosphorylate S208 and S242. Pyrin forms an inflammasome when mutant or in response to bacterial modification of the GTPase RhoA. We found that RhoA activated the serine-threonine kinases PKN1 and PKN2 that bind and phosphorylate pyrin. Phosphorylated pyrin bound to 14-3-3 proteins, regulatory proteins that in turn blocked the pyrin inflammasome. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ARHGEF17 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.549 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260542 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP26 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.629 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260481 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MCF2L | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.619 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260559 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GMIP | down-regulates activity
gtpase-activating protein
|
RHOA |
0.643 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260505 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP11A | down-regulates activity
gtpase-activating protein
|
RHOA |
0.548 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260466 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF3 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.777 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260530 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TAGAP | down-regulates activity
gtpase-activating protein
|
RHOA |
0.447 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260523 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF19 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.635 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260543 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
|
PFN1 |
0.569 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253109 |
|
|
Homo sapiens |
MDA-MB-231 Cell |
| pmid |
sentence |
| 22820501 |
We find that the small GTPase Rho regulates R-cadherin adherens junction formation via Dia1 (also known as p140mDia) and profilin-1-mediated signaling pathway. The role played by Rho in regulating R-cadherin is underscored by the fact that constitutively active RhoA(Q63L) induces R-cadherin junction formation in MDA-MB-231 cells.|Data presented thus far demonstrated that Rho, Dia1, and profilin-1 were required for R-cadherin junction formation in N480 cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PLEKHG6 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.583 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260567 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GNA13 | up-regulates
binding
|
RHOA |
0.573 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-192111 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23450633 |
Ga12/13 recruitment of rho-gefs causes rhoa activation and f-actin assembly, which promotes lats1/lat2 inactivation by an unknown, but myosin-independent mechanism. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP1 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.864 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260458 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ADGRG1 | up-regulates activity
binding
|
RHOA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253981 |
|
|
|
|
| pmid |
sentence |
| 24949629 |
Like many other adhesion GPCRs, GPR56 is cleaved via a GPCR autoproteolysis-inducing (GAIN) domain into N- and C-terminal fragments (GPR56N and GPR56C); | We demonstrate that ligand binding releases GPR56N from the membrane-bound GPR56C and triggers the association of GPR56C with lipid rafts and RhoA activation. |
|
| Publications: |
1 |
| + |
ARHGAP22 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260476 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
KCTD10 | down-regulates quantity
binding
|
RHOA |
0.269 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264237 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19782033 |
BACURDs form ubiquitin ligase complexes, which selectively ubiquitinate RhoA, with Cul3. Our studies reveal a previously unknown mechanism for controlling RhoA degradation and regulating RhoA function in various biological contexts, which involves a Cul3/BACURD ubiquitin ligase complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NET1 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.832 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260561 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP5 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.828 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260461 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP35 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.893 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260492 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TNFAIP1 | down-regulates quantity
binding
|
RHOA |
0.35 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264235 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19782033 |
BACURDs form ubiquitin ligase complexes, which selectively ubiquitinate RhoA, with Cul3. Our studies reveal a previously unknown mechanism for controlling RhoA degradation and regulating RhoA function in various biological contexts, which involves a Cul3/BACURD ubiquitin ligase complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MYO9A | down-regulates activity
gtpase-activating protein
|
RHOA |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260508 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP31 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.529 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260488 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STARD8 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.557 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260519 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TGFBR1 | up-regulates activity
|
RHOA |
0.64 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-227499 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19726546 |
Thus, TGF-_1 rapidly stimulates activity of both RhoA and Rac1 and this activation requires ALK5/T_RI kinase activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MCF2 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.754 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260556 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CHRNA7 | up-regulates activity
binding
|
RHOA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253985 |
|
|
|
|
| pmid |
sentence |
| 27167578 |
Here, we demonstrate a role for α7 nAChR/G protein interaction in the activation of the small (monomeric) RhoA GTPase leading to cytoskeletal changes during neurite growth. Treatment of PC12 cells with the α7 nAChR agonist choline or PNU-282987 was associated with an increase in RhoA activity and an inhibition in neurite growth. |
|
| Publications: |
1 |
| + |
RAP1GDS1 | up-regulates
binding
|
RHOA |
0.633 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-171347 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21242305 |
Smggds is a guanine nucleotide exchange factor that specifically activates rhoa and rhoc |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF5 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.616 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260533 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
binding
|
ROCK1 |
0.81 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-196740 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25010901 |
Rho-associated coiled-coil containing kinases (ROCK) were originally identified as effectors of the RhoA small GTPase |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199542 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23151663 |
Planar cell polarity (pcp) signalling triggers activation of the small gtpases rhoa and rac1, which in turn activate rho kinase (rock) and jun-n-terminal kinase (jnk), respectively, leading to actin polymerization and microtubule stabilization. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
RHOA | up-regulates
binding
|
PLD1 |
0.697 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-84953 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11102529 |
Our results demonstrate that direct stimulation of pld1 in vivo by rhoa |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
binding
|
PKN2 |
0.833 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275466 |
|
|
Mus musculus |
Bone Marrow-derived Macrophage |
| pmid |
sentence |
| 27270401 |
PKNs bind to human pyrin and phosphorylate S208 and S242. Pyrin forms an inflammasome when mutant or in response to bacterial modification of the GTPase RhoA. We found that RhoA activated the serine-threonine kinases PKN1 and PKN2 that bind and phosphorylate pyrin. Phosphorylated pyrin bound to 14-3-3 proteins, regulatory proteins that in turn blocked the pyrin inflammasome. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
RHOA | up-regulates 
|
F-actin_assembly |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-192114 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23450633 |
Ga12/13 recruitment of rho-gefs causes rhoa activation and f-actin assembly, which promotes lats1/lat2 inactivation by an unknown, but myosin-independent mechanism. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF12 | up-regulates
guanine nucleotide exchange factor
|
RHOA |
0.905 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-84657 |
|
|
Homo sapiens |
Leukemia Cell |
| pmid |
sentence |
| 11094164 |
Here, we show that larg can activate rho in vivo |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF4 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.667 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260531 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PARD6/SMURF1 | down-regulates
ubiquitination
|
RHOA |
0.675 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-227492 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15761148 |
The Par6-Smurf1 complex then mediates the localized ubiquitination of RhoA to enable the TGF_-dependent dissolution of tight junctions during EMT. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VAV3 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.711 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260584 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF2 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.806 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260529 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP8 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.427 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260463 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates
relocalization
|
FHL2 |
0.348 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-114071 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11847121 |
Here, we show that stimulation of the rho pathway induces translocation of the transcriptional lim-only coactivator fhl2 to the nucleus and subsequent activation of fhl2- and androgen receptor-dependent genes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NGEF | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.674 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260562 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF12 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.905 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260539 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STARD13 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.644 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260521 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP23 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.548 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260479 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZD3 | up-regulates activity
binding
|
RHOA |
0.344 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-167865 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20828404 |
Upon ligand binding, non-canonical wnt signaling controls tissue polarity and cell movement through the activation of rhoa, c-jun n-terminal kinase (jnk), and nemo-like kinase (nlk) signaling cascades. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP29 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.552 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260484 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP11B | down-regulates activity
gtpase-activating protein
|
RHOA |
0.502 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260467 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PREX2 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.406 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260570 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP10 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.668 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260465 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DYNLL1 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.273 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260501 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DVL1 | up-regulates activity
binding
|
RHOA |
0.624 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-258971 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27571105 |
Although there are other activators of PCP, Wnt5a can activate the PCP pathway by forming a complex with Fzd and Ror2 receptors, activating DVL, which in turn activates Rho-family small GTPases, including RhoA and Rac, and their downstream effectors, Rho-associated protein kinase (ROCK), the actin-binding protein, Filamin A and c-Jun N-terminal protein kinase (JNK) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
ARHGEF15 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.571 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260540 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF28 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.755 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260546 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GNAQ | up-regulates
binding
|
RHOA |
0.576 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-156534 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17606614 |
Recently, the dbl-family guanine nucleotide exchange factor (gef) p63rhogef/geft has been described as a novel mediator of galpha(q/11) signaling to rhoa based on its ability to synergize with galpha(q/11) resulting in enhanced rhoa signaling in cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VAV2 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.75 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260582 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARAP2 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.513 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260453 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP6 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.58 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260462 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP30 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.449 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260485 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
|
DIAPH1 |
0.785 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253108 |
|
|
Homo sapiens |
MDA-MB-231 Cell |
| pmid |
sentence |
| 22820501 |
We find that the small GTPase Rho regulates R-cadherin adherens junction formation via Dia1 (also known as p140mDia) and profilin-1-mediated signaling pathway. The role played by Rho in regulating R-cadherin is underscored by the fact that constitutively active RhoA(Q63L) induces R-cadherin junction formation in MDA-MB-231 cells.|Data presented thus far demonstrated that Rho, Dia1, and profilin-1 were required for R-cadherin junction formation in N480 cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ECT2 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.712 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260550 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AKAP13 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.744 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260527 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF11 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.902 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260538 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
KCTD13 | down-regulates quantity
binding
|
RHOA |
0.377 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264236 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19782033 |
BACURDs form ubiquitin ligase complexes, which selectively ubiquitinate RhoA, with Cul3. Our studies reveal a previously unknown mechanism for controlling RhoA degradation and regulating RhoA function in various biological contexts, which involves a Cul3/BACURD ubiquitin ligase complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PLEKHG5 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.771 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260566 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF25 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.835 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260544 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DRAM2 | up-regulates quantity by expression
transcriptional regulation
|
RHOA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259142 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30755245 |
Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). Furthermore, DRAM2 overexpression increased the expression of proteins RAC1, RHOA, RHOC, ROCK1, and decreased RHOB expression, all of which are cell migration factors. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF10 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.525 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260535 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP28 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.504 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260483 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RHOA | up-regulates activity
binding
|
MAPK8 |
0.827 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-258974 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 8824197 |
We found that in the human kidney epithelial cell line, 293T, Cdc42 and all Rho proteins, RhoA, RhoB, and RhoC, but not Rac or Ras can induce activation of JNK. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
ARHGAP19 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.568 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260471 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
COMMD5 | up-regulates activity
|
RHOA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261690 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30021164 |
We found that RhoA activity is decreased following COMMD5 depletion, suggesting that COMMD5 regulates cytoskeleton organization through RhoA signaling.We found that RhoA activity is decreased following COMMD5 depletion, suggesting that COMMD5 regulates cytoskeleton organization through RhoA signaling. However, the dynamics of Rho GTPase activities are highly complex and tightly regulated in order to achieve their specific subcellular localization (Marjoram et al., 2014); thus, the mechanism by which COMMD5 directly or indirectly regulates the activity of RhoA needs to be investigated further. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF1 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.831 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260528 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGAP21 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.628 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260475 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
OPHN1 | up-regulates activity
gtpase-activating protein
|
RHOA |
0.635 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268397 |
|
|
Homo sapiens |
Neuron |
| pmid |
sentence |
| 12932438 |
OPHN-1 colocalized with the actin cytoskeleton in neuronal and glial cells. We have previously shown that OPHN1 stimulates GTPases activity of RhoA, Cdc42, and Rac1 in vitro |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CUL3 | down-regulates quantity by destabilization
ubiquitination
|
RHOA |
0.396 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264238 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19782033 |
BACURDs form ubiquitin ligase complexes, which selectively ubiquitinate RhoA, with Cul3. Our studies reveal a previously unknown mechanism for controlling RhoA degradation and regulating RhoA function in various biological contexts, which involves a Cul3/BACURD ubiquitin ligase complex. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DAAM1 | up-regulates activity
binding
|
RHOA |
0.822 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-185268 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19365405 |
B-catenin-independent wnt signaling can activate rho family gtpases through at least two mechanisms: (1) direct activation of rac1 by dvl;and (2) activation of rhoa via dvl-associated activator of morphogenesis-1 (daam1), possibly through the weak-similarity guaninenucleotide exchange factor (wgef)1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
ARHGAP20 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.708 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260472 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RASGRF2 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.553 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260573 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARHGEF1 | up-regulates
guanine nucleotide exchange factor
|
RHOA |
0.831 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-77914 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10836144 |
Rhogefs catalyze the exchange of gdp for gtp and thereby activate rho. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Muscle, Smooth Muscle |
| + |
ARHGEF25 | up-regulates
guanine nucleotide exchange factor
|
RHOA |
0.835 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236885 |
|
|
Mus musculus |
C2C12 Cell, Myoblast |
| pmid |
sentence |
| 16314529 |
Exogenous expression of geft promotes myogenesis ofc2c12 cells via activation of rhoa, rac1, and cdc42 and their downstream effector proteins, while a dominant negative mutant of geft inhibits this process. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ARHGAP40 | down-regulates activity
gtpase-activating protein
|
RHOA |
0.431 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260496 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
VAV1 | up-regulates activity
guanine nucleotide exchange factor
|
RHOA |
0.75 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260580 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 32203420 |
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity among regulators, but also that the inactivating RhoGAPs are less selective than the activating RhoGEFs (p-value=0.02)(Supplementary Table 2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
RHOA
- 
- 