Relation Results

Summary

Name MAPK3
Full Name Mitogen-activated protein kinase 3
Synonyms MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1 | ERK1, PRKM3
Primary ID P27361
Links - -
Type protein
Relations 419
Pathways Glucocorticoid receptor Signaling
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs ...
View More

Viewer

Type: Score: Layout: SPV 
0.4670.4330.6970.3020.5220.3480.4050.6270.450.270.5910.3610.380.4150.70.3020.6350.3570.20.8510.3190.3070.3850.520.3260.3020.270.5310.2490.3550.2790.20.20.3770.3210.7230.710.20.440.5940.530.7220.2840.3710.7420.5680.6310.5090.4910.3440.3320.4160.3450.2690.3490.6280.20.2760.5880.5540.5880.550.7810.6220.4470.20.5890.4120.4040.3170.4270.5740.7110.2670.3150.6240.7110.5120.4290.7090.3150.3950.2730.4250.3970.5970.20.2660.20.2620.5540.650.5010.510.3960.3430.3040.6870.2710.5540.6440.70.70.490.3560.6060.3270.7770.20.6910.3790.20.2920.3320.20.7250.4770.5480.2540.7130.3020.3590.4670.3230.6990.2680.2790.2970.6850.4180.2650.20.5680.5450.4450.3070.3820.7350.6380.20.20.7810.7010.7450.20.5110.660.20.4260.3410.3770.6610.6640.5640.3560.20.3390.3140.280.2820.2920.7320.5460.3020.3670.2670.4210.2970.6560.5540.4390.4660.20.5740.4010.5960.530.390.7620.6140.3170.620.4320.4690.3530.2950.3130.4940.80.5160.7110.3150.5950.6960.4280.4820.5930.3870.4390.8510.4040.5040.4510.7540.29MAPK3JUNDHDAC6ESR1LIFRERBB4POU5F1PPARGSOS1SREBF1NCOA1PPARATAL1CICTHRBTP53GRB10BRAFTOB1BAZ1BDUSP6POLR2APTTG1RCAN1NFATC1PCBP2MAGEA11APBB1MITFPAX5NR3C2LIN28AKARS1MCRIP1CEBPAETV6RPS6KRPS6KA1NUP50ABI1SPHK1NR3C1RPS6KA3DEPTORCCDC6SMAD2STMN1GJA1RXRAMAPTHSPB8ALOX5CREMCIITANOXA1HNRNPKRAF1TFCP2AMPHFOXO3RUNX2FOXOPGRDUSP1HSF1PCYT1AIRX2ELK1BCL6PPP2R5CCDK1NCF1RPS6KA5FOSASB2BAG3GAB1CASP8SPHK2CTTNARRB1XPO5STK11METTL3KRT8SREBF2RPS6KB1MRTFAKLC1SYN3EPAS1CAPN2PLA2G4ANANOGJAK2NUP153PMLMYBTSC2WWC1LCKSP1IRS1MYCTHSTMN2GAB2EPS8JUNRRN3HIF1AGTF2IGRK2NFATC4TWIST1ARHGAP26BCL2L11RPTORBCL2PDE4DSTAT3SP3NOS2CALD1FGFR1STAT5AIL16GABBR1GSK3BPTPN7PLCB1MED1SULT4A1UBTFCASP9MCL1LATCDKN1BMAP2K2PPP2CAMEK1/2DUSP4MAP2K1ERK1/2MBPCEBPBTRPV3SMAD4PAK1TCF3PTPRRETS1MKNK1RPS3CADSHOC2SPIBSCNN1BSCNN1GARHGEF2ATF2EGFRMAZADAM17EWSR1LIPECTNND1DUSP3RETPTPRJPP1RPS6KA4CDC25ALAMTOR3mTORC1PEA15SMAD3LRP6SHC1THRBADGSK3B/Axin/APCE2F1DUSP23WDR8317beta-estradiolSMAD1RPS6KA2BRD4FN1DUSP9NCKIPSDMAPKAPK2FGF2SOX9PTPRBMKNK2PPP1CADUSP5MYL1

Search Tools

Refine search:

  • by mechanism


  • by effect
  • by organism:


  • by tissue:


  • by cell-line

▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ MAPK3up-regulates img/direct-activation.png phosphorylation JUND 0.467
Identifier Residue Sequence Organism Cell Line
SIGNOR-196034 Ser100 LGLLKLAsPELERLI Homo sapiens
pmid sentence
Menin binds the jun family transcription factor jund and inhibits its transcriptional activity. The menin-jund interaction blocks jun n-terminal kinase (jnk)-mediated jund phosphorylation and suppresses jund-induced transcription. We found a role for phosphorylation of the ser100 residue of jund;jund phosphorylation were prevented by inhibitors of calcium, calmodulin, or erk1/2 kinase.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation HDAC6 0.433
Identifier Residue Sequence Organism Cell Line
SIGNOR-202698 Ser1035 DHQTPPTsPVQGTTP Homo sapiens
pmid sentence
Histone deacetylase 6 (hdac6) is well known for its ability to promote cell migrationextracellular signal-regulated kinase (erk) phosphorylates histone deacetylase 6 (hdac6) at serine 1035 to stimulate cell migrationwe have identified two novel erk-mediated phosphorylation sites: threonine 1031 and serine 1035 in hdac6. Both sites were phosphorylated by erk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-202702 Thr1031 ASSTDHQtPPTSPVQ Homo sapiens
pmid sentence
Histone deacetylase 6 (hdac6) is well known for its ability to promote cell migrationextracellular signal-regulated kinase (erk) phosphorylates histone deacetylase 6 (hdac6) at serine 1035 to stimulate cell migrationwe have identified two novel erk-mediated phosphorylation sites: threonine 1031 and serine 1035 in hdac6. Both sites were phosphorylated by erk1
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation ESR1 0.697
Identifier Residue Sequence Organism Cell Line
SIGNOR-156860 Ser104 FPPLNSVsPSPLMLL Homo sapiens HeLa Cell
pmid sentence
In several estrogen response element-containing genes, the s118a mutation strongly reduced induction by e(2), and u0126 did not further reduce expression. Here, we show that serines 104 (s104) and 106 (s106) are also phosphorylated by mapk in vitro and upon stimulation of mapk activity in vivo.Phosphorylation at serines 104 and 106 by erk1/2 mapk is important for estrogen receptor-alpha activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-178141 Ser104 FPPLNSVsPSPLMLL Homo sapiens
pmid sentence
In several estrogen response element-containing genes, the s118a mutation strongly reduced induction by e(2), and u0126 did not further reduce expression. Here, we show that serines 104 (s104) and 106 (s106) are also phosphorylated by mapk in vitro and upon stimulation of mapk activity in vivo.Phosphorylation at serines 104 and 106 by erk1/2 mapk is important for estrogen receptor-alpha activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-178145 Ser106 PLNSVSPsPLMLLHP Homo sapiens
pmid sentence
In several estrogen response element-containing genes, the s118a mutation strongly reduced induction by e(2), and u0126 did not further reduce expression. Here, we show that serines 104 (s104) and 106 (s106) are also phosphorylated by mapk in vitro and upon stimulation of mapk activity in vivo.Phosphorylation at serines 104 and 106 by erk1/2 mapk is important for estrogen receptor-alpha activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-156864 Ser106 PLNSVSPsPLMLLHP Homo sapiens HeLa Cell
pmid sentence
In several estrogen response element-containing genes, the s118a mutation strongly reduced induction by e(2), and u0126 did not further reduce expression. Here, we show that serines 104 (s104) and 106 (s106) are also phosphorylated by mapk in vitro and upon stimulation of mapk activity in vivo.Phosphorylation at serines 104 and 106 by erk1/2 mapk is important for estrogen receptor-alpha activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-156868 Ser118 LHPPPQLsPFLQPHG Homo sapiens HeLa Cell
pmid sentence
In several estrogen response element-containing genes, the s118a mutation strongly reduced induction by e(2), and u0126 did not further reduce expression. Here, we show that serines 104 (s104) and 106 (s106) are also phosphorylated by mapk in vitro and upon stimulation of mapk activity in vivo.
Publications: 5 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation LIFR 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-32757 Ser1044 WNLVSPDsPRSIDSN Homo sapiens
pmid sentence
Thus, our results identify the human lifr as a substrate for mapk and suggest a mechanism of heterologous receptor regulation of lifr signaling occurring at ser-1044.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation ERBB4 0.522
Identifier Residue Sequence Organism Cell Line
SIGNOR-277449 Ser1051 NRSEIGHsPPPAYTP Homo sapiens HEK-293 Cell
pmid sentence
We also identified Ser-1026 as an ErbB4-specific ERK target site in the CYT-1 region. Moreover, double mutations (Thr-674/Ser-1026 to Ala) significantly upregulated ErbB4 activation, indicating that Thr-674 and Ser-1026 are cooperatively involved in negative feedback regulation. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-277448 Thr699 TELVEPLtPSGTAPN Homo sapiens HEK-293 Cell
pmid sentence
We also identified Ser-1026 as an ErbB4-specific ERK target site in the CYT-1 region. Moreover, double mutations (Thr-674/Ser-1026 to Ala) significantly upregulated ErbB4 activation, indicating that Thr-674 and Ser-1026 are cooperatively involved in negative feedback regulation. 
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation POU5F1 0.348
Identifier Residue Sequence Organism Cell Line
SIGNOR-192101 Ser111 ESNSDGAsPEPCTVT Homo sapiens
pmid sentence
Phosphorylation of this site downregulates nanog, sox2, rex1 and upregulates bmp4, gata6, ddlx5.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation PPARG 0.405
Identifier Residue Sequence Organism Cell Line
SIGNOR-179404 Ser112 AIKVEPAsPPYYSEK Homo sapiens
pmid sentence
Moreover, the inhibition of erks 1 and 2 with a mek inhibitor, u1026, lead to an inhibition in the decay of ppargamma proteins, indicating that serine phosphorylation influences the degradation of ppargamma in fat cells.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation SOS1 0.627
Identifier Residue Sequence Organism Cell Line
SIGNOR-43939 Ser1132 TLPHGPRsASVSSIS Homo sapiens
pmid sentence
In this report, we describe the identification of five MAP kinase sites (S-1137, S-1167, S-1178, S-1193, and S-1197) on hSos1.Replacing the MAP kinase phosphorylation sites with alanine residues results in an increase in the binding affinity of Grb2 to hSos1
Identifier Residue Sequence Organism Cell Line
SIGNOR-43943 Ser1167 ESAPAESsPSKIMSK Homo sapiens
pmid sentence
In this report, we describe the identification of five map kinase sites (s-1137, s-1167, s-1178, s-1193, and s-1197) on hsos1
Identifier Residue Sequence Organism Cell Line
SIGNOR-43947 Ser1178 IMSKHLDsPPAIPPR Homo sapiens
pmid sentence
In this report, we describe the identification of five map kinase sites (s-1137, s-1167, s-1178, s-1193, and s-1197) on hsos1
Identifier Residue Sequence Organism Cell Line
SIGNOR-43951 Ser1193 QPTSKAYsPRYSISD Homo sapiens
pmid sentence
In this report, we describe the identification of five map kinase sites (s-1137, s-1167, s-1178, s-1193, and s-1197) on hsos1
Identifier Residue Sequence Organism Cell Line
SIGNOR-26338 Homo sapiens
pmid sentence
For example, inactivation of sos through phosphorylation by the downstream mapk
Publications: 5 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation SREBF1 0.45
Identifier Residue Sequence Organism Cell Line
SIGNOR-80096 Ser117 YPSMPAFsPGPGIKE Homo sapiens Hep-G2 Cell
pmid sentence
Map kinases erk1/2 phosphorylate sterol regulatory element-binding protein (srebp)-1a at serine 117 in vitro. mutation of serine 117 to alanine abolished erk2-mediated phosphorylation in vitro and the map kinase-related transcriptional activation of srebp-1a by insulin and platelet-derived growth factor in vivo.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation NCOA1 0.27
Identifier Residue Sequence Organism Cell Line
SIGNOR-91139 Ser1185 GTPPASTsPFSQLAA Homo sapiens Prostate Gland Cancer Cell
pmid sentence
Mapk also directly phosphorylates src-1 at thr1179 and ser1185. Phosphorylation of src-1 by mitogen-activated protein kinase (mapk) is required for optimal progesterone receptor-dependent transcription and for functional cooperation with camp response element-binding protein-binding protein
Identifier Residue Sequence Organism Cell Line
SIGNOR-91143 Thr1179 NYGTNPGtPPASTSP Homo sapiens
pmid sentence
Mapk also directly phosphorylates src-1 at thr1179 and ser1185. Phosphorylation of src-1 by mitogen-activated protein kinase (mapk) is required for optimal progesterone receptor-dependent transcription and for functional cooperation with camp response element-binding protein-binding protein
Publications: 2 Organism: Homo Sapiens
Pathways:Glucocorticoid receptor Signaling
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation PPARA 0.591
Identifier Residue Sequence Organism Cell Line
SIGNOR-249473 Ser12 ESPLCPLsPLEAGDL Homo sapiens
pmid sentence
We now demonstrate that amino acids 1-92 of hPPARalpha contain an activation function (AF)-1-like domain, which is further activated by insulin through a pathway involving the mitogen-activated protein kinases p42 and p44. Further analysis of the amino-terminal region of PPARalpha revealed that the insulin-induced trans-activation occurs through the phosphorylation of two mitogen-activated protein kinase sites at positions 12 and 21, both of which are conserved across evolution.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249474 Ser21 LEAGDLEsPLSEEFL Homo sapiens Hep-G2 Cell
pmid sentence
We now demonstrate that amino acids 1-92 of hPPARalpha contain an activation function (AF)-1-like domain, which is further activated by insulin through a pathway involving the mitogen-activated protein kinases p42 and p44. Further analysis of the amino-terminal region of PPARalpha revealed that the insulin-induced trans-activation occurs through the phosphorylation of two mitogen-activated protein kinase sites at positions 12 and 21, both of which are conserved across evolution.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation TAL1 0.361
Identifier Residue Sequence Organism Cell Line
SIGNOR-116153 Ser122 DGRMVQLsPPALAAP Homo sapiens
pmid sentence
We report here that the important proangiogenic stimulus hypoxia stimulates phosphorylation, ubiquitination, and proteasomal breakdown of tal1 in endothelial cells. A specific serine in the putative transactivation domain of the protein, ser122, is preferentially phosphorylated by mapk in vitro.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation CIC 0.38
Identifier Residue Sequence Organism Cell Line
SIGNOR-169879 Ser1409 SAPEDPTsPKRKMRR Homo sapiens Melanoma Cell
pmid sentence
Specifically, 14-3-3 binds to p90(rsk)-phosphorylated ser?_??_ Of capic?_A thereby modulating dna binding to its hmg (high-mobility group) box, whereas erk phosphorylations prevent binding of a c-terminal nls (nuclear localization sequence) to importin ?4 (kpna3)[...] These results suggest that erk phosphorylation of ser1382 and ser1409 masks the nls and prevents its binding to kpna3
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation THRB 0.415
Identifier Residue Sequence Organism Cell Line
SIGNOR-102224 Ser142 IQKNLHPsYSCKYEG Homo sapiens
pmid sentence
We concluded that serine 142 of the tr dbd is the likely site of phosphorylation by t(4)-activated mapk and that the docking site on tr for activated mapk includes residues 128-133 (kgffrr), a basic amino acid-enriched motif novel for mapk substrates. Tr mutations in the proposed mapk docking domain and at residue 142 modulated t(4)-conditioned shedding of co-repressor and recruitment of co-activator proteins by the receptor, and they altered transcriptional activity of tr in a thyroid hormone response element-luciferase reporter assay.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation TP53 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-100270 Ser15 PSVEPPLsQETFSDL Homo sapiens
pmid sentence
Mutant p53 is constitutively phosphorylated at serine 15 in uv-induced mouse skin tumors: involvement of erk1/2 map kinase.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation GRB10 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-138171 Ser150 PELCGPGsPPVLTPG Homo sapiens
pmid sentence
Phosphorylation of grb10 by mitogen-activated protein kinase: identification of ser150 and ser476 of human grb10zeta as major phosphorylation sitesreplacing ser(150) and ser(476) with alanines reduced the inhibitory effect of human grb10zeta on insulin-stimulated irs1 tyrosine phosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-138175 Ser476 MNILGSQsPLHPSTL Homo sapiens
pmid sentence
Phosphorylation of grb10 by mitogen-activated protein kinase: identification of ser150 and ser476 of human grb10zeta as major phosphorylation sitesreplacing ser(150) and ser(476) with alanines reduced the inhibitory effect of human grb10zeta on insulin-stimulated irs1 tyrosine phosphorylation
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation BRAF 0.635
Identifier Residue Sequence Organism Cell Line
SIGNOR-259921 Ser151 VARSNPKsPQKPIVR Homo sapiens Melanoma Cell
pmid sentence
We show that overactivation of the MAPK pathway, induced by the oncogenic Ras in melanoma, induces constitutive phosphorylation of BRAF on Ser151 by ERK, which inhibits NRAS-BRAF interaction
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation TOB1 0.357
Identifier Residue Sequence Organism Cell Line
SIGNOR-88728 Ser152 PASSVSSsPSPPFGH Homo sapiens
pmid sentence
Biochemical analyses have then shown that erk mapk (erk2) and jnk/sapk (jnk2) bind to and phosphorylate tob in vitro. Erk catalyzes the phosphorylation more efficiently than jnk
Identifier Residue Sequence Organism Cell Line
SIGNOR-91059 Ser154 SSVSSSPsPPFGHSA Homo sapiens T-lymphocyte
pmid sentence
Biochemical analyses have then shown that erk mapk (erk2) and jnk/sapk (jnk2) bind to and phosphorylate tob in vitro. Erk catalyzes the phosphorylation more efficiently than jnk
Identifier Residue Sequence Organism Cell Line
SIGNOR-88732 Ser154 SSVSSSPsPPFGHSA Homo sapiens
pmid sentence
Tob is rapidly phosphorylated at Ser 152, Ser 154, and Ser 164 by Erk1 and Erk2 upon growth-factor stimulation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-88736 Ser164 FGHSAAVsPTFMPRS Homo sapiens
pmid sentence
Biochemical analyses have then shown that erk mapk (erk2) and jnk/sapk (jnk2) bind to and phosphorylate tob in vitro. Erk catalyzes the phosphorylation more efficiently than jnk
Identifier Residue Sequence Organism Cell Line
SIGNOR-91063 Ser164 FGHSAAVsPTFMPRS Homo sapiens T-lymphocyte
pmid sentence
Biochemical analyses have then shown that erk mapk (erk2) and jnk/sapk (jnk2) bind to and phosphorylate tob in vitro. Erk catalyzes the phosphorylation more efficiently than jnk
Publications: 5 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation BAZ1B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-188164 Ser158 KSDGACDsPSSDKEN Homo sapiens
pmid sentence
Wstf, a specific component of two chromatin remodeling complexes (swi/snf-type winac and iswi-type wich), was phosphorylated by the stimulation of mapk cascades in vitro and in vivo. Ser-158 residue in the wac (wstf/acf1/cbpq46) domain, located close to the n terminus of wstf, was identified as a major phosphorylation target
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation DUSP6 0.851
Identifier Residue Sequence Organism Cell Line
SIGNOR-132975 Ser159 DGSCSSSsPPLPVLG Homo sapiens
pmid sentence
Phosphorylation of serines 159 and 197 by erk1/2 enhances proteasomal degradation of mkp-3
Identifier Residue Sequence Organism Cell Line
SIGNOR-132979 Ser197 SATDSDGsPLSNSQP Homo sapiens
pmid sentence
Phosphorylation of serines 159 and 197 by erk1/2 enhances proteasomal degradation of mkp-3
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation POLR2A 0.319
Identifier Residue Sequence Organism Cell Line
SIGNOR-120172 Ser1619 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120176 Ser1626 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120180 Ser1647 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120184 Ser1654 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120188 Ser1668 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120192 Ser1675 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120196 Ser1696 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120200 Ser1717 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120204 Ser1724 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120208 Ser1738 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120212 Ser1766 SPSYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120216 Ser1787 SPNYSPTsPSYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120220 Ser1864 SPKYSPTsPKYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120224 Ser1871 SPKYSPTsPKYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120228 Ser1882 SPTSPTYsPTTPKYS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120232 Ser1892 TPKYSPTsPTYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120236 Ser1899 SPTYSPTsPVYTPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120240 Ser1913 SPKYSPTsPTYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120244 Ser1920 SPTYSPTsPKYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120248 Ser1927 SPKYSPTsPTYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120252 Ser1934 SPTYSPTsPKGSTYS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120256 Ser1944 GSTYSPTsPGYSPTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120260 Ser1951 SPGYSPTsPTYSLTS Homo sapiens
pmid sentence
Erk1/2 are major ser-5 kinases after h2o2 treatment. These results suggest that subsequent to h2o2 treatment, the ser-5-phosphorylated form, but not the ser-2-phosphorylated form or the unphosphorylated form, is targeted for rapid proteasomal degradation through its ubiquitination.
Publications: 23 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation PTTG1 0.307
Identifier Residue Sequence Organism Cell Line
SIGNOR-79519 Ser165 LFQLGPPsPVKMPSP Homo sapiens
pmid sentence
Pttg is phosphorylated in vitro on ser(162) by map kinase and this phosphorylation site plays an essential role in pttg transactivation function.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation RCAN1 0.385
Identifier Residue Sequence Organism Cell Line
SIGNOR-249478 Ser167 FLISPPAsPPVGWKQ Mus musculus C2C12 Cell
pmid sentence
Consensus phosphorylation sites for p42/44 MAPK and GSK-3 are present in the SP repeat of MCIP1 at serine 112 and serine 108, respectively |Several endogenous proteins are capable of inhibiting the catalytic activity of calcineurin. Modulatory calcineurin interacting protein 1 (MCIP1) is unique among these proteins on the basis of its pattern of expression and its function in a negative feedback loop to regulate calcineurin activity. Here we show that MCIP1 can be phosphorylated by MAPK and glycogen synthase kinase-3 and that phosphorylated MCIP1 is a substrate for calcineurin.
Publications: 1 Organism: Mus Musculus
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation NFATC1 0.52
Identifier Residue Sequence Organism Cell Line
SIGNOR-74564 Ser172 YRDPSCLsPASSLSS Homo sapiens
pmid sentence
We show that jnk, erk, and p38 physically associate with the nfatc n-terminal regulatory domain and can directly phosphorylate functionally important residues involved in regulating nfatc subcellular localization, namely ser(172) and the conserved nfatc ser-pro repeats.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates quantity by stabilization img/direct-activation.png phosphorylation PCBP2 0.326
Identifier Residue Sequence Organism Cell Line
SIGNOR-262914 Ser173 MLETLSQsPPKGVTI Homo sapiens K-562 Cell
pmid sentence
We also identified 4 hnRNP-E2 MAPKERK1/2 phosphorylation sites and demonstrated that hnRNP-E2 is a bona fide MAPKERK1/2 substrate and that MAPKERK1/2-dependent phosphorylation of hnRNP-E2 at these amino acid residues is essential for increased hnRNP-E2 expression in BCR/ABL-expressing cells. Serine/threonine to alanine substitution abolishes hnRNP-E2 phosphorylation and markedly decreases its stability in BCR/ABL-expressing myeloid precursors. Consistent with the existence of a BCR/ABL-MAPK pathway that posttranslationally regulates hnRNP-E2 expression, sequence analysis of hnRNP-E2 revealed the presence of 4 consensus ERK phosphorylation sites (S/T-P)35,36 at amino acid residues 173, 189, 213, and 272 (Figure 2B).
Identifier Residue Sequence Organism Cell Line
SIGNOR-262915 Ser189 YRPKPSSsPVIFAGG Homo sapiens
pmid sentence
We also identified 4 hnRNP-E2 MAPKERK1/2 phosphorylation sites and demonstrated that hnRNP-E2 is a bona fide MAPKERK1/2 substrate and that MAPKERK1/2-dependent phosphorylation of hnRNP-E2 at these amino acid residues is essential for increased hnRNP-E2 expression in BCR/ABL-expressing cells. Serine/threonine to alanine substitution abolishes hnRNP-E2 phosphorylation and markedly decreases its stability in BCR/ABL-expressing myeloid precursors. Consistent with the existence of a BCR/ABL-MAPK pathway that posttranslationally regulates hnRNP-E2 expression, sequence analysis of hnRNP-E2 revealed the presence of 4 consensus ERK phosphorylation sites (S/T-P)35,36 at amino acid residues 173, 189, 213, and 272 (Figure 2B).
Identifier Residue Sequence Organism Cell Line
SIGNOR-262916 Ser272 FSGIESSsPEVKGYW Homo sapiens
pmid sentence
We also identified 4 hnRNP-E2 MAPKERK1/2 phosphorylation sites and demonstrated that hnRNP-E2 is a bona fide MAPKERK1/2 substrate and that MAPKERK1/2-dependent phosphorylation of hnRNP-E2 at these amino acid residues is essential for increased hnRNP-E2 expression in BCR/ABL-expressing cells. Serine/threonine to alanine substitution abolishes hnRNP-E2 phosphorylation and markedly decreases its stability in BCR/ABL-expressing myeloid precursors. Consistent with the existence of a BCR/ABL-MAPK pathway that posttranslationally regulates hnRNP-E2 expression, sequence analysis of hnRNP-E2 revealed the presence of 4 consensus ERK phosphorylation sites (S/T-P)35,36 at amino acid residues 173, 189, 213, and 272 (Figure 2B).
Identifier Residue Sequence Organism Cell Line
SIGNOR-262917 Thr213 SASFPHTtPSMCLNP Homo sapiens K-562 Cell
pmid sentence
We also identified 4 hnRNP-E2 MAPKERK1/2 phosphorylation sites and demonstrated that hnRNP-E2 is a bona fide MAPKERK1/2 substrate and that MAPKERK1/2-dependent phosphorylation of hnRNP-E2 at these amino acid residues is essential for increased hnRNP-E2 expression in BCR/ABL-expressing cells. Serine/threonine to alanine substitution abolishes hnRNP-E2 phosphorylation and markedly decreases its stability in BCR/ABL-expressing myeloid precursors. Consistent with the existence of a BCR/ABL-MAPK pathway that posttranslationally regulates hnRNP-E2 expression, sequence analysis of hnRNP-E2 revealed the presence of 4 consensus ERK phosphorylation sites (S/T-P)35,36 at amino acid residues 173, 189, 213, and 272 (Figure 2B).
Publications: 4 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MAGEA11 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-188466 Ser174 ESPSPPQsPQEESFS Homo sapiens Melanoma Cell
pmid sentence
Mage-11 ser-174 appears to be a post-translational regulatory site phosphorylated by erk1, based on the inhibitory effect of the s174a mutation in the context of shorter ar nh2-terminal fragments (19), and the greater transcriptional activity of gal-mage-11 fusion proteins containing the s174d phosphomimetic.
Publications: 1 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation APBB1 0.27
Identifier Residue Sequence Organism Cell Line
SIGNOR-120467 Ser175 EEEEDLSsPPGLPEP Homo sapiens
pmid sentence
Thus, fe65 has at least two apparently disparate functions and may also be involved in the pathogenesis of alzheimer's disease. The mechanisms by which fe65 trafficking and metabolism are regulated to fulfil these different roles are unclear. Our findings reported here, which demonstrate that fe65 is a phosphoprotein that is targeted by erk1/2 and which identify four in vivo phosphorylation sites, provide one possible mechanism whereby functional diversity might be achieved.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120475 Ser287 WEPPGRAsPSQGSSP Homo sapiens
pmid sentence
Thus, fe65 has at least two apparently disparate functions and may also be involved in the pathogenesis of alzheimer's disease. The mechanisms by which fe65 trafficking and metabolism are regulated to fulfil these different roles are unclear. Our findings reported here, which demonstrate that fe65 is a phosphoprotein that is targeted by erk1/2 and which identify four in vivo phosphorylation sites, provide one possible mechanism whereby functional diversity might be achieved.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120479 Ser347 TFPAQSLsPEPLPQE Homo sapiens
pmid sentence
Thus, fe65 has at least two apparently disparate functions and may also be involved in the pathogenesis of alzheimer's disease. The mechanisms by which fe65 trafficking and metabolism are regulated to fulfil these different roles are unclear. Our findings reported here, which demonstrate that fe65 is a phosphoprotein that is targeted by erk1/2 and which identify four in vivo phosphorylation sites, provide one possible mechanism whereby functional diversity might be achieved.
Identifier Residue Sequence Organism Cell Line
SIGNOR-120483 Thr709 PKRLGAHtP Homo sapiens
pmid sentence
Thus, fe65 has at least two apparently disparate functions and may also be involved in the pathogenesis of alzheimer's disease. The mechanisms by which fe65 trafficking and metabolism are regulated to fulfil these different roles are unclear. Our findings reported here, which demonstrate that fe65 is a phosphoprotein that is targeted by erk1/2 and which identify four in vivo phosphorylation sites, provide one possible mechanism whereby functional diversity might be achieved.
Publications: 4 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation MITF 0.531
Identifier Residue Sequence Organism Cell Line
SIGNOR-249620 Ser180 PGSSAPNsPMAMLTL
pmid sentence
More interestingly, ERK-dependent phosphorylation of MITF at Ser 73 is essential for MITF ubiquitinilation and degradation (87). Putting together all these findings, it can be proposed that MAPK activation inhibits melanogenesis due to an increased MITF degradation which is dependent on the MAPK-induced MITF phosphorylation and ubiquitinilation. In summary, although the phosphorylation of MITF at Ser73 increases its intrinsic transcriptional activity, this phosphorylation also targets MITF to the proteasome for its degradation. Consequently, the decrease in MITF levels leads to a down-regulation of melanogenic enzymes expression and to an inhibition of melanogenesis.
Publications: 1
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation PAX5 0.249
Identifier Residue Sequence Organism Cell Line
SIGNOR-269086 Ser189
Ser283		 SGILGITsPSADTNK
DMKANLAsPTPADIG		 Homo sapiens RAMOS (RA.1) Cell
pmid sentence
In this study, we demonstrated that PAX5 was phosphorylated by ERK1/2 in vitro and in vivo at serines 189 and 283. This phosphorylation attenuated the transcriptional repression of BLIMP1 by PAX5.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation NR3C2 0.355
Identifier Residue Sequence Organism Cell Line
SIGNOR-276102 Ser196 EKSPSVCsPLNMTSS Homo sapiens M1 Melanoma Cell
pmid sentence
 Taken together, these data suggest that ERK1/2 directly phosphorylates the MR on several serine residues present in its NTD, that the upward shift of MR is mainly due to receptor phosphorylation, and finally that these sites represent the major aldosterone-inducible targets for MR phosphorylation.MR phosphorylation limits the transcriptional activity.Taken together, these results provide evidence that MR phosphorylation plays a role in aldosterone-mediated ubiquitylation and degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276111 Ser227 FGSFPVHsPITQGTP Homo sapiens M1 Melanoma Cell
pmid sentence
 Taken together, these data suggest that ERK1/2 directly phosphorylates the MR on several serine residues present in its NTD, that the upward shift of MR is mainly due to receptor phosphorylation, and finally that these sites represent the major aldosterone-inducible targets for MR phosphorylation.MR phosphorylation limits the transcriptional activity.Taken together, these results provide evidence that MR phosphorylation plays a role in aldosterone-mediated ubiquitylation and degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276112 Ser238 QGTPLTCsPNVENRG Homo sapiens M1 Melanoma Cell
pmid sentence
 Taken together, these data suggest that ERK1/2 directly phosphorylates the MR on several serine residues present in its NTD, that the upward shift of MR is mainly due to receptor phosphorylation, and finally that these sites represent the major aldosterone-inducible targets for MR phosphorylation.MR phosphorylation limits the transcriptional activity.Taken together, these results provide evidence that MR phosphorylation plays a role in aldosterone-mediated ubiquitylation and degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276108 Ser263 NVGSPLSsPLSSMKS Homo sapiens M1 Melanoma Cell
pmid sentence
 Taken together, these data suggest that ERK1/2 directly phosphorylates the MR on several serine residues present in its NTD, that the upward shift of MR is mainly due to receptor phosphorylation, and finally that these sites represent the major aldosterone-inducible targets for MR phosphorylation.MR phosphorylation limits the transcriptional activity.Taken together, these results provide evidence that MR phosphorylation plays a role in aldosterone-mediated ubiquitylation and degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276110 Ser287 SVKSPVSsPNNVTLR Homo sapiens M1 Melanoma Cell
pmid sentence
 Taken together, these data suggest that ERK1/2 directly phosphorylates the MR on several serine residues present in its NTD, that the upward shift of MR is mainly due to receptor phosphorylation, and finally that these sites represent the major aldosterone-inducible targets for MR phosphorylation.MR phosphorylation limits the transcriptional activity.Taken together, these results provide evidence that MR phosphorylation plays a role in aldosterone-mediated ubiquitylation and degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276106 Ser361 TLRDVVPsPDTQEKG Homo sapiens M1 Melanoma Cell
pmid sentence
 Taken together, these data suggest that ERK1/2 directly phosphorylates the MR on several serine residues present in its NTD, that the upward shift of MR is mainly due to receptor phosphorylation, and finally that these sites represent the major aldosterone-inducible targets for MR phosphorylation.MR phosphorylation limits the transcriptional activity.Taken together, these results provide evidence that MR phosphorylation plays a role in aldosterone-mediated ubiquitylation and degradation.
Publications: 6 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation LIN28A 0.279
Identifier Residue Sequence Organism Cell Line
SIGNOR-277337 Ser200 EEEEEIHsPTLLPEA Homo sapiens HeLa Cell
pmid sentence
Here we show that Lin28a is directly phosphorylated by ERK1/2 kinases at Ser-200. 
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation KARS1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-186125 Ser207 PYEITLLsPCLHMLP Homo sapiens
pmid sentence
Lysrs serves as a key signaling molecule in the immune response by regulating gene expression. Lysrs was phosphorylated on serine 207 in a mapk-dependent manner, released from the multisynthetase complex, and translocated into the nucleus.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation MCRIP1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-264773 Ser21 KRTSSPRsPPSSSEI Homo sapiens
pmid sentence
When phosphorylated by ERK, MCRIP1 dissociates from CtBP, allowing CtBP to interact with ZEB1. In this manner, the CtBP co-repressor complex is recruited to, and silences, the E-cadherin promoter by inducing chromatin modifications.| While substitution of S4 or S18 with Ala did not affect the phosphorylation of MCRIP1 by ERK, substitution of either S21 or T30 significantly reduced MCRIP1 phosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-264772 Thr30 PSSSEIFtPAHEENV Homo sapiens
pmid sentence
When phosphorylated by ERK, MCRIP1 dissociates from CtBP, allowing CtBP to interact with ZEB1. In this manner, the CtBP co-repressor complex is recruited to, and silences, the E-cadherin promoter by inducing chromatin modifications.| While substitution of S4 or S18 with Ala did not affect the phosphorylation of MCRIP1 by ERK, substitution of either S21 or T30 significantly reduced MCRIP1 phosphorylation
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation CEBPA 0.377
Identifier Residue Sequence Organism Cell Line
SIGNOR-120570 Ser21 PMSSHLQsPPHAPSS Homo sapiens Monocyte
pmid sentence
Ccaat/enhancer-binding protein alpha (c/ebpalpha) is one of the key transcription factors that mediate lineage specification and differentiation of multipotent myeloid progenitors into mature granulocytes.Here we report that inducers of monocyte differentiation inhibit the alternate cell fate choice, that of granulopoiesis, through inhibition of c/ebpalpha. This inhibition is mediated by extracellular signal-regulated kinases 1 and/or 2 (erk1/2), which interact with c/ebpalpha through an fxfp docking site and phosphorylate serine 21.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation ETV6 0.321
Identifier Residue Sequence Organism Cell Line
SIGNOR-123656 Ser213 DNMIRRLsPAERAQG Mus musculus
pmid sentence
Tel became phosphorylated by erk on two serine residues, ser213 and ser257, in the internal domain between the hlh and ets domains. Tel lost its abilities to repress transcription through the phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-260085 Ser257 ESHPKPSsPRQESTR Mus musculus NIH-3T3 Cell
pmid sentence
Tel became phosphorylated by erk on two serine residues, ser213 and ser257, in the internal domain between the hlh and ets domains. Tel lost its abilities to repress transcription through the phosphorylation.
Publications: 2 Organism: Mus Musculus
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation RPS6K 0.723
Identifier Residue Sequence Organism Cell Line
SIGNOR-252758 Ser221 DHEKKAYsFCGTVEY Chlorocebus aethiops
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252759 Ser363 TSRTPKDsPGIPPSA Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252756 Ser380 HQLFRGFsFVATGLM Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252761 Ser732 RRVRKLPsTTL Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252755 Thr359 DTEFTSRtPKDSPGI Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252757 Thr573 AENGLLMtPCYTANF Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252760 Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity.Erk-activates the rsk family of serine/threonine kinases,rsk1, rsk2, and rsk3.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252762 Mus musculus
pmid sentence
The pp90rsk phosphothreonine content paralleled the ERK1 activity more closely than the phosphoserine level. These results provide compelling evidence that in fibroblasts and PC12 cells ERK1 plays a direct role in the phosphorylation of pp90rsk and that pp90rsk represents a physiologically relevant substrate of extracellular-regulated kinases
Publications: 8 Organism: Chlorocebus Aethiops, Homo Sapiens, Mus Musculus
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation RPS6KA1 0.71
Identifier Residue Sequence Organism Cell Line
SIGNOR-219332 Ser221 DHEKKAYsFCGTVEY Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-219337 Ser363 TSRTPKDsPGIPPSA Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-219341 Ser380 HQLFRGFsFVATGLM Chlorocebus aethiops
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-219345 Ser732 RRVRKLPsTTL Chlorocebus aethiops
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-219349 Thr359 DTEFTSRtPKDSPGI Chlorocebus aethiops
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-219353 Thr573 AENGLLMtPCYTANF Chlorocebus aethiops COS-1 Cell
pmid sentence
Several lines of evidence indicate that the mapkap-k1 isoforms are also activated by mapks in vivo via the ras-dependent protein kinase cascade that is triggered by growth factors or tumor-promoting phorbol esters, such as phorbol 12-myristate 13-acetate (pma). here we identify six sites in mapkap-k1a that become phosphorylated in transfected cos-1 cells. The inactive form of mapkap-k1a in unstimulated cells is partially phosphorylated at ser222 and ser733. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of thr360, ser364, thr574, and ser381 and increases the phosphorylation of ser222 and ser733.
Identifier Residue Sequence Organism Cell Line
SIGNOR-102648 Homo sapiens
pmid sentence
Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity.Erk-activates the rsk family of serine/threonine kinases,rsk1, rsk2, and rsk3.
Identifier Residue Sequence Organism Cell Line
SIGNOR-38999 Mus musculus
pmid sentence
The pp90rsk phosphothreonine content paralleled the ERK1 activity more closely than the phosphoserine level. These results provide compelling evidence that in fibroblasts and PC12 cells ERK1 plays a direct role in the phosphorylation of pp90rsk and that pp90rsk represents a physiologically relevant substrate of extracellular-regulated kinases
Publications: 8 Organism: Chlorocebus Aethiops, Homo Sapiens, Mus Musculus
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation NUP50 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-188151 Ser221 KVAAETQsPSLFGST Homo sapiens
pmid sentence
Erk phosphorylates nup50 at ser221 and ser315 erk phosphorylation of the fg repeat region of nup50 reduced its affinity for importin-beta family proteins, importin-beta and transportin.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation ABI1 0.44
Identifier Residue Sequence Organism Cell Line
SIGNOR-172881 Ser225 ARLGSQHsPGRTASL Homo sapiens
pmid sentence
We show that erk colocalizes with the wrc at lamellipodial leading edges and directly phosphorylates two wrc components: wave2 and abi1.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation SPHK1 0.594
Identifier Residue Sequence Organism Cell Line
SIGNOR-118550 Ser225 VGSKTPAsPVVVQQG Homo sapiens
pmid sentence
Activation of sphingosine kinase 1 by erk1/2-mediated phosphorylation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation NR3C1 0.53
Identifier Residue Sequence Organism Cell Line
SIGNOR-154409 Ser226 IDENCLLsPLAGEDD in vitro
pmid sentence
Cyclin-dependent kinase (CDK) and mitogen-activated protein kinase (MAPK) phosphorylate the rat glucocorticoid receptor in vitro at distinct sites that together correspond to the major phosphorylated receptor residues observed in vivo; MAPK phosphorylates receptor residues threonine 171 and serine 246, whereas multiple CDK complexes modify serines 224 and 232.|MAPKs and CDKs exert opposite effects on receptor transcriptional enhancement. From our results, we speculate that activators of the MAPK pathway, such as growth factors, insulin, and certain oncoproteins, or inhibitors of CDK function, such as tumor growth factor beta (TGF_), p21, and p27, might attenuate receptor-induced transcrip- tional responses. In contrast, negative regulators of MAPK, such as pKA, as well as activators of CDK, such as the cyclins or CAKs, should potentiate receptor action.
Publications: 1 Organism: In Vitro
Pathways:Glucocorticoid receptor Signaling
+ MAPK3up-regulates img/direct-activation.png phosphorylation RPS6KA3 0.722
Identifier Residue Sequence Organism Cell Line
SIGNOR-81460 Ser227 DHEKKAYsFCGTVEY Homo sapiens
pmid sentence
We have generated two monoclonal antibodies that recognize two phosphorylated sites, p-ser227 and p-thr577, in the n- and c-terminal kinase domains of rsk2, respectively. phosphorylation and activation of rsk2 by uv light involves the erk pathway
Identifier Residue Sequence Organism Cell Line
SIGNOR-81464 Thr577 AENGLLMtPCYTANF Homo sapiens
pmid sentence
We have generated two monoclonal antibodies that recognize two phosphorylated sites, p-ser227 and p-thr577, in the n- and c-terminal kinase domains of rsk2, respectively. phosphorylation and activation of rsk2 by uv light involves the erk pathway
Identifier Residue Sequence Organism Cell Line
SIGNOR-184583 Homo sapiens
pmid sentence
Erk-activates the rsk family of serine/threonine kinases,rsk1, rsk2, and rsk3.
Publications: 3 Organism: Homo Sapiens
+ MAPK3up-regulates quantity by stabilization img/direct-activation.png phosphorylation DEPTOR 0.284
Identifier Residue Sequence Organism Cell Line
SIGNOR-277587 Ser235 MELLNEKsPSSQETH in vitro
pmid sentence
Screening the DEPTOR interactome identified that the association of USP-7 deubiquitinase with DEPTOR was dependent upon S235 phosphorylation. Inhibition of USP-7 activity resulted in DEPTOR polyubiquitination and degradation. A scansite search suggested that ERK1 may be responsible for S235 phosphorylation, which was confirmed through the use of inhibitors, ERK1 knockdown, and an in vitro kinase assay.
Publications: 1 Organism: In Vitro
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation CCDC6 0.371
Identifier Residue Sequence Organism Cell Line
SIGNOR-276003 Ser244 QPVSAPPsPRDISME Homo sapiens HEK-293 Cell
pmid sentence
We have characterized the H4(D10S170) gene product, showing that it is a ubiquitously expressed 55 KDa nuclear and cytosolic protein that is phosphorylated following serum stimulation. This phosphorylation was found to depend on mitogen-activated protein kinase (MAPK) Erk1/2 activity and to be associated to the relocation of H4(D10S170) from the nucleus to the cytosol. S244 is the major target residue of ERK1
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation SMAD2 0.742
Identifier Residue Sequence Organism Cell Line
SIGNOR-66759 Ser245 NQSMDTGsPAELSPT Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-182988 Ser245 NQSMDTGsPAELSPT Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-91730 Ser245 NQSMDTGsPAELSPT Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-91734 Ser250 TGSPAELsPTTLSPV Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-182992 Ser250 TGSPAELsPTTLSPV Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-66763 Ser250 TGSPAELsPTTLSPV Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-182996 Ser255 ELSPTTLsPVNHSLD Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-91738 Ser255 ELSPTTLsPVNHSLD Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-66767 Ser255 ELSPTTLsPVNHSLD Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-91742 Thr220 QSNYIPEtPPPGYIS Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-183000 Thr220 QSNYIPEtPPPGYIS Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-66771 Thr220 QSNYIPEtPPPGYIS Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-183004 Thr8 MSSILPFtPPVVKRL Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-91746 Thr8 MSSILPFtPPVVKRL Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-66775 Thr8 MSSILPFtPPVVKRL Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Publications: 15 Organism: Homo Sapiens
Tissue: Lung, Breast, Lung
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation STMN1 0.568
Identifier Residue Sequence Organism Cell Line
SIGNOR-249482 Ser25 QAFELILsPRSKESV Homo sapiens
pmid sentence
Stress-induced stathmin phosphorylation is not de- pendent on ERK. Stathmin is also known to be phos- phorylated by ERK on Ser-25 and Ser-38 (17). Thus, it is possible that ERK phosphorylates stathmin in 293 cells|In subsequent reports (28, 29) it was shown that phosphorylation of stathmin blocks its ability to destabilize MTs.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249483 Ser38 SVPEFPLsPPKKKDL Homo sapiens
pmid sentence
Stress-induced stathmin phosphorylation is not de- pendent on ERK. Stathmin is also known to be phos- phorylated by ERK on Ser-25 and Ser-38 (17). Thus, it is possible that ERK phosphorylates stathmin in 293 cells|In subsequent reports (28, 29) it was shown that phosphorylation of stathmin blocks its ability to destabilize MTs.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation GJA1 0.631
Identifier Residue Sequence Organism Cell Line
SIGNOR-249465 Ser255 HATSGALsPAKDCGS Homo sapiens
pmid sentence
These studies confirm that connexin-43 is a MAP kinase substrate in vivo and that phosphorylation on Ser255, Ser279, and/or Ser282 initiates the down-regulation of gap junctional communication. Studies with connexin-43 mutants suggest that MAP kinase phosphorylation at one or more of the tandem Ser279/Ser282 sites is sufficient to disrupt gap junctional intercellular communication.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249466 Ser279 SSPTAPLsPMSPPGY Homo sapiens
pmid sentence
These studies confirm that connexin-43 is a MAP kinase substrate in vivo and that phosphorylation on Ser255, Ser279, and/or Ser282 initiates the down-regulation of gap junctional communication. Studies with connexin-43 mutants suggest that MAP kinase phosphorylation at one or more of the tandem Ser279/Ser282 sites is sufficient to disrupt gap junctional intercellular communication.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249467 Ser282 TAPLSPMsPPGYKLV Homo sapiens HeLa Cell
pmid sentence
These studies confirm that connexin-43 is a MAP kinase substrate in vivo and that phosphorylation on Ser255, Ser279, and/or Ser282 initiates the down-regulation of gap junctional communication. Studies with connexin-43 mutants suggest that MAP kinase phosphorylation at one or more of the tandem Ser279/Ser282 sites is sufficient to disrupt gap junctional intercellular communication.
Publications: 3 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation RXRA 0.509
Identifier Residue Sequence Organism Cell Line
SIGNOR-88662 Ser260 NMGLNPSsPNDPVTN Homo sapiens
pmid sentence
In colon cancer cells, the Ras/mitogen‐activated protein kinase (MAPK) pathway phosphorylates RXRalpha, which impairs its function as a heterodimeric partner for PPARgamma|A point‐mutated RXRalpha T82A/S260A, which mimics the unphosphorylated form of RXRalpha, can form a heterodimer with PPARgamma and thereby activate target gene expression by binding to the PPRE
Identifier Residue Sequence Organism Cell Line
SIGNOR-262959 Thr82 HSMSVPTtPTLGFST Homo sapiens
pmid sentence
In colon cancer cells, the Ras/mitogen‐activated protein kinase (MAPK) pathway phosphorylates RXRalpha, which impairs its function as a heterodimeric partner for PPARgamma|A point‐mutated RXRalpha T82A/S260A, which mimics the unphosphorylated form of RXRalpha, can form a heterodimer with PPARgamma and thereby activate target gene expression by binding to the PPRE
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation MAPT (isoform 2) 0.491
Identifier Residue Sequence Organism Cell Line
SIGNOR-275434 Ser267 RVQSKIGsLDNITHV in vitro
pmid sentence
We have studied the relationship between the phosphorylation oftau by several kinases (MARK, PKA, MAPK, GSK3) and its assembly into PHFs. By contrast, MARK and PKA phosphorylate several sites within the repeats (notably theKXGS motifs including Ser262, Ser324, and Ser356, plus Ser320); in addition PKA phosphorylates somesites in the flanking domains, notably Ser214. This type of phosphorylation strongly reduces tau’s affinityfor microtubules, and at the same time inhibits tau’s assembly into PHFs.
Publications: 1 Organism: In Vitro
+ MAPK3up-regulates img/direct-activation.png phosphorylation HSPB8 0.344
Identifier Residue Sequence Organism Cell Line
SIGNOR-197932 Ser27 PFRDSPLsSRLLDDG Homo sapiens
pmid sentence
Hsp22 is phosphorylated by protein kinase c (at residues ser(14) and thr(63)) and by p44 mitogen-activated protein kinase (at residues ser(27) and thr(87)). Concerning the possible function of hsp22, no definitive conclusions can be drawn with the available data, although its function might be to bind to and modulate the activity of hsp27.Some Studies claimed that phosphorylation is required for the translocation
Identifier Residue Sequence Organism Cell Line
SIGNOR-197936 Thr87 GVPAEGRtPPPFPGE Homo sapiens
pmid sentence
Hsp22 is phosphorylated by protein kinase c (at residues ser(14) and thr(63)) and by p44 mitogen-activated protein kinase (at residues ser(27) and thr(87)). Concerning the possible function of hsp22, no definitive conclusions can be drawn with the available data, although its function might be to bind to and modulate the activity of hsp27.Some Studies claimed that phosphorylation is required for the translocation
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation HSPB8 0.344
Identifier Residue Sequence Organism Cell Line
SIGNOR-107676 Ser27 PFRDSPLsSRLLDDG Homo sapiens
pmid sentence
Hsp22 is phosphorylated by protein kinase c (at residues ser(14) and thr(63)) and by p44 mitogen-activated protein kinase (at residues ser(27) and thr(87)). Concerning the possible function of hsp22, no definitive conclusions can be drawn with the available data, although its function might be to bind to and modulate the activity of hsp27.Some Studies claimed that phosphorylation is required for the translocation
Identifier Residue Sequence Organism Cell Line
SIGNOR-107680 Thr87 GVPAEGRtPPPFPGE Homo sapiens
pmid sentence
Hsp22 is phosphorylated by protein kinase c (at residues ser(14) and thr(63)) and by p44 mitogen-activated protein kinase (at residues ser(27) and thr(87)). Concerning the possible function of hsp22, no definitive conclusions can be drawn with the available data, although its function might be to bind to and modulate the activity of hsp27.Some Studies claimed that phosphorylation is required for the translocation
Publications: 2 Organism: Homo Sapiens
Tissue: Muscle
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation ALOX5 0.332
Identifier Residue Sequence Organism Cell Line
SIGNOR-264441 Ser272 CSLERQLsLEQEVQQ Homo sapiens HeLa Cell
pmid sentence
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-264440 Ser664 QLPYYYLsPDRIPNS Homo sapiens HeLa Cell
pmid sentence
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation CREM 0.416
Identifier Residue Sequence Organism Cell Line
SIGNOR-275978 Ser277 ASPGSLHsPQQLAEE Mus musculus AtT-20 Cell
pmid sentence
 The MAPKs extracellular signal-regulated kinases 1 and 2 physically interact with ICER and mediated the phosphorylation of ICER on a critical serine residue (Ser-41). A mutant form of ICER in which Ser-41 was substituted by alanine had a half-life 4-5 h longer than its wild-type counterpart. This alteration in stability was due to the inability of the Ser-41-mutant ICER to be efficiently ubiquitinated and degraded via the ubiquitin-proteasome pathway. 
Publications: 1 Organism: Mus Musculus
+ MAPK3up-regulates img/direct-activation.png phosphorylation CIITA 0.345
Identifier Residue Sequence Organism Cell Line
SIGNOR-150545 Ser280 TVHGLPTsPDRPGST Homo sapiens T-lymphocyte, Macrophage
pmid sentence
We found that in these cells, lipopolysaccharide stimulates the expression of mhc ii genes via the activation of erk1/2, which is mediated by toll-like receptor 4. Erk1/2 then phosphorylates the serine at position 357, which is located in a degron of ciita isoform 1 that leads to its monoubiquitylation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation NOXA1 0.269
Identifier Residue Sequence Organism Cell Line
SIGNOR-164231 Ser282 VGKQAPLsPGLPAMG Homo sapiens
pmid sentence
Accumulating evidence indicates that protein phosphorylation regulates nox activity. In this report, we show that serine282 residue of nox activator 1 (noxa1) is phosphorylated by erk in response to egf resulting in desensitization of nox1 activity
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation HNRNPK 0.349
Identifier Residue Sequence Organism Cell Line
SIGNOR-145375 Ser284 RRDYDDMsPRRGPPP Homo sapiens
pmid sentence
Erk phosphorylation drives cytoplasmic accumulation of hnrnp-k and inhibition of mrna translation mitogen-activated protein kinase/extracellular-signal-regulated kinase (mapk/erk) efficiently phosphorylates hnrnp-k both in vitro and in vivo at serines 284 and 353.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation HNRNPK 0.349
Identifier Residue Sequence Organism Cell Line
SIGNOR-105238 Ser284 RRDYDDMsPRRGPPP Homo sapiens
pmid sentence
Erk phosphorylation drives cytoplasmic accumulation of hnrnp-k and inhibition of mrna translation mitogen-activated protein kinase/extracellular-signal-regulated kinase (mapk/erk) efficiently phosphorylates hnrnp-k both in vitro and in vivo at serines 284 and 353.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation CIITA 0.345
Identifier Residue Sequence Organism Cell Line
SIGNOR-160617 Ser288 PDRPGSTsPFAPSAT Homo sapiens
pmid sentence
In this study we show that the extracellular signal-regulated kinases 1 and 2 (erk1/2) interact directly with ciita, targeting serine residues in the amino terminus of the protein, including serine 288. These data suggest a model whereby erk1/2-mediated phosphorylation of ciita down-regulates ciita activity by priming it for nuclear export, thus providing a means for cells to tightly regulate the extent of antigen presentation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation RAF1 0.628
Identifier Residue Sequence Organism Cell Line
SIGNOR-143688 Ser289 RSHSESAsPSALSSS Mus musculus
pmid sentence
Here, we identify six residues of Raf-1 (S29, S43, S289, S296, S301, and S642) that become hyperphosphorylated in a manner coincident with Raf-1 inactivation. | Five of the identified sites are proline-directed targets of activated ERK, and phosphorylation of all six sites requires MEK signaling, indicating a negative feedback mechanism. Hyperphosphorylation of these six sites inhibits the Ras/Raf-1 interaction and desensitizes Raf-1 to additional stimuli.|FLAG-Raf-1 phosphorylated by activated ERK2
Identifier Residue Sequence Organism Cell Line
SIGNOR-143692 Ser296 SPSALSSsPNNLSPT Mus musculus
pmid sentence
Here, we identify six residues of Raf-1 (S29, S43, S289, S296, S301, and S642) that become hyperphosphorylated in a manner coincident with Raf-1 inactivation. | Five of the identified sites are proline-directed targets of activated ERK, and phosphorylation of all six sites requires MEK signaling, indicating a negative feedback mechanism. Hyperphosphorylation of these six sites inhibits the Ras/Raf-1 interaction and desensitizes Raf-1 to additional stimuli.|FLAG-Raf-1 phosphorylated by activated ERK2
Publications: 2 Organism: Mus Musculus
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation TFCP2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-184176 Ser291 TYVNNSPsPGFNSSH Homo sapiens
pmid sentence
We previously established that phosphorylation of lsf in early g1 at ser-291 and ser-309 inhibits its transcriptional activity and that dephosphorylation later in g1 is required for its reactivation. At the peak activities of erk and cyclin c/cdk2 in early g1, lsf is efficiently phosphorylated on ser-291 and ser-309.
Identifier Residue Sequence Organism Cell Line
SIGNOR-184180 Ser309 SLGEGNGsPNHQPEP Homo sapiens
pmid sentence
We previously established that phosphorylation of lsf in early g1 at ser-291 and ser-309 inhibits its transcriptional activity and that dephosphorylation later in g1 is required for its reactivation. At the peak activities of erk and cyclin c/cdk2 in early g1, lsf is efficiently phosphorylated on ser-291 and ser-309.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation AMPH 0.276
Identifier Residue Sequence Organism Cell Line
SIGNOR-126867 Ser293 PAPARPRsPSQTRKG Homo sapiens
pmid sentence
Thus, we propose that mapk phosphorylation of amphiphysin1 controls ngf receptor/trka-mediated endocytosis by terminating the amphiphysin1-ap-2 interaction.Our results indicate that phosphorylation of amphiphysin 1 at ser-285 and/or ser-293 affects the function of amphiphysin1.Mapk phosphorylation of ser-285 and ser-293 could modulate the interaction between prd and ap-2, resulting in the dissociation of amphiphysin1 from ap-2.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation FOXO3 0.588
Identifier Residue Sequence Organism Cell Line
SIGNOR-184569 Ser294 QLSKWPGsPTSRSSD Homo sapiens
pmid sentence
Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway
Identifier Residue Sequence Organism Cell Line
SIGNOR-184573 Ser344 QDDDAPLsPMLYSSS Homo sapiens
pmid sentence
Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway
Identifier Residue Sequence Organism Cell Line
SIGNOR-184577 Ser425 TKGSGLGsPTSSFNS Homo sapiens
pmid sentence
Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway
Publications: 3 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation RUNX2 0.554
Identifier Residue Sequence Organism Cell Line
SIGNOR-188343 Ser294 DPRQAQSsPPWSYDQ Homo sapiens
pmid sentence
In this study, we identified two phosphorylation sites in runx2 at ser301 and ser319 that are required for mapk-dependent activation of runx2 transcriptional activity and osteoblast differentiation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-188347 Ser312 SYLSQMTsPSIHSTT Homo sapiens
pmid sentence
In this study, we identified two phosphorylation sites in runx2 at ser301 and ser319 that are required for mapk-dependent activation of runx2 transcriptional activity and osteoblast differentiation.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation FOXO 0.588
Identifier Residue Sequence Organism Cell Line
SIGNOR-252961 Ser294 QLSKWPGsPTSRSSD Homo sapiens
pmid sentence
Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway
Identifier Residue Sequence Organism Cell Line
SIGNOR-252962 Ser344 QDDDAPLsPMLYSSS Homo sapiens
pmid sentence
Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway
Identifier Residue Sequence Organism Cell Line
SIGNOR-252963 Ser425 TKGSGLGsPTSSFNS Homo sapiens
pmid sentence
Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway
Publications: 3 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation PGR 0.55
Identifier Residue Sequence Organism Cell Line
SIGNOR-74716 Ser294 APMAPGRsPLATTVM Homo sapiens Breast Cancer Cell
pmid sentence
Specifically, down-regulation of mature prs occurs by a mechanism in which ligand binding activates pr phosphorylation by mapks at a unique serine residue, which then targets the receptors for degradation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation AMPH 0.276
Identifier Residue Sequence Organism Cell Line
SIGNOR-126871 Ser295 PARPRSPsQTRKGPP Homo sapiens
pmid sentence
Thus, we propose that mapk phosphorylation of amphiphysin1 controls ngf receptor/trka-mediated endocytosis by terminating the amphiphysin1-ap-2 interaction.Our results indicate that phosphorylation of amphiphysin 1 at ser-285 and/or ser-293 affects the function of amphiphysin1.Mapk phosphorylation of ser-285 and ser-293 could modulate the interaction between prd and ap-2, resulting in the dissociation of amphiphysin1 from ap-2.
Publications: 1 Organism: Homo Sapiens
Tissue: Brain
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation DUSP1 0.781
Identifier Residue Sequence Organism Cell Line
SIGNOR-141605 Ser296 KQRRSIIsPNFSFMG Homo sapiens
pmid sentence
The dual-specificity mapk phosphatase mkp-1/cl100/dusp1 is an inducible nuclear protein controlled by p44/42 mapk (erk1/2) in a negative feedback mechanism to inhibit kinase activity. Here, we report on the molecular basis for a novel positive feedback mechanism to sustain erk activation by triggering mkp-1 proteolysis. Active erk2 docking to the def motif (fxfp, residues 339-342) of n-terminally truncated mkp-1 in vitro initiated phosphorylation at the ser(296)/ser(323) domain
Identifier Residue Sequence Organism Cell Line
SIGNOR-141609 Ser323 HCSAEAGsPAMAVLD Homo sapiens
pmid sentence
The dual-specificity mapk phosphatase mkp-1/cl100/dusp1 is an inducible nuclear protein controlled by p44/42 mapk (erk1/2) in a negative feedback mechanism to inhibit kinase activity. Here, we report on the molecular basis for a novel positive feedback mechanism to sustain erk activation by triggering mkp-1 proteolysis. Active erk2 docking to the def motif (fxfp, residues 339-342) of n-terminally truncated mkp-1 in vitro initiated phosphorylation at the ser(296)/ser(323) domain
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation RAF1 0.628
Identifier Residue Sequence Organism Cell Line
SIGNOR-143696 Ser301 SSSPNNLsPTGWSQP Homo sapiens
pmid sentence
Using mass spectrometry, we identified raf-1 phosphorylation on three sp motif sites: s289/s296/s301. These sites were phosphorylated by extracellular signal-regulated kinase (erk)-1 in vitro, and their phosphorylation in vivo was dependent on endogenous erk activity. Functionally, erk-1 expression sustains raf-1 activation in a manner dependent on raf-1 phosphorylation on the identified sites, and s289/296/301a substitution markedly decreases the in vivo activity of raf-1 s259a.
Identifier Residue Sequence Organism Cell Line
SIGNOR-64172 Homo sapiens
pmid sentence
Mapkerk1/2 is also able to phopshorylate the egf receptor, the ras exchange factor sos, mkkkraf1, and mkkmek1. The phosphorylation of each of these proteins by mapkerk1/2 is believed to reduce their catalytic activity. previous studies have shown that phosphorylation is required for raf-1 activation, and here, we identify six phosphorylation sites that contribute to the downregulation of raf-1 after mitogen stimulation. Five of the identified sites are proline-directed targets of activated erk
Identifier Residue Sequence Organism Cell Line
SIGNOR-133345 Homo sapiens
pmid sentence
Mapkerk1/2 is also able to phopshorylate the egf receptor, the ras exchange factor sos, mkkkraf1, and mkkmek1. The phosphorylation of each of these proteins by mapkerk1/2 is believed to reduce their catalytic activity. previous studies have shown that phosphorylation is required for raf-1 activation, and here, we identify six phosphorylation sites that contribute to the downregulation of raf-1 after mitogen stimulation. Five of the identified sites are proline-directed targets of activated erk
Publications: 3 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation HSF1 0.622
Identifier Residue Sequence Organism Cell Line
SIGNOR-44999 Ser307 EPPSPPQsPRVEEAS Homo sapiens
pmid sentence
Sequential phosphorylation of hsf1 by mitogen-activated protein kinase and glycogen synthase kinase 3 at ser-303 and ser-307 represses transcriptional activation by heat shock factor-1.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation PCYT1A 0.447
Identifier Residue Sequence Organism Cell Line
SIGNOR-134841 Ser315 GRMLQAIsPKQSPSS Homo sapiens
pmid sentence
Oxysterols inhibit phosphatidylcholine synthesis via erk docking and phosphorylation of ctp:phosphocholine cytidylyltransferase. Mutagenesis of ser315 within cctalpha was both required and sufficient to confer significant resistance to 22-hc/9-cis-ra inhibition of ptdcho synthesis.
Publications: 1 Organism: Homo Sapiens
Tissue: Lung
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation NUP50 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-187378 Ser315 TQSKPVSsPFPTKPL Homo sapiens
pmid sentence
Erk phosphorylates nup50 at ser221 and ser315 phosphorylation of nup50 reduces affinity for importin-beta
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation IRX2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-263060 Ser317 TPQGSRTsPGAPPPA in vitro
pmid sentence
To identify the phosphorylated residue, we introduced a serine-to-alanine substitution at residues 294 and 326 and a threonine-to-alanine substitution at residue 331 in Irx2(291–356). Erk1 phosphorylated S294A and T331A, but not S326A (Fig. 4b), indicating that Ser326 is the bona fide MAP kinase target.
Identifier Residue Sequence Organism Cell Line
SIGNOR-263061 Ser325 PGAPPPAsKPKLWSL in vitro
pmid sentence
To identify the phosphorylated residue, we introduced a serine-to-alanine substitution at residues 294 and 326 and a threonine-to-alanine substitution at residue 331 in Irx2(291–356). Erk1 phosphorylated S294A and T331A, but not S326A (Fig. 4b), indicating that Ser326 is the bona fide MAP kinase target.
Publications: 2 Organism: In Vitro
+ MAPK3up-regulates img/direct-activation.png phosphorylation ELK1 0.589
Identifier Residue Sequence Organism Cell Line
SIGNOR-34653 Ser324 RDLELPLsPSLLGGP Homo sapiens
pmid sentence
Erki phosphorylates five c-terminal sites in elk-i (s324, t336, s383, s389 and s422) with varying degrees of efficiency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-34657 Ser383 IHFWSTLsPIAPRSP Homo sapiens
pmid sentence
Erki phosphorylates five c-terminal sites in elk-i (s324, t336, s383, s389 and s422) with varying degrees of efficiency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-34661 Ser389 LSPIAPRsPAKLSFQ Homo sapiens
pmid sentence
Erki phosphorylates five c-terminal sites in elk-i (s324, t336, s383, s389 and s422) with varying degrees of efficiency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-34665 Ser422 LSTPVVLsPGPQKP Homo sapiens
pmid sentence
Erki phosphorylates five c-terminal sites in elk-i (s324, t336, s383, s389 and s422) with varying degrees of efficiency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-34669 Thr336 GGPGPERtPGSGSGS Homo sapiens
pmid sentence
Erki phosphorylates five c-terminal sites in elk-i (s324, t336, s383, s389 and s422) with varying degrees of efficiency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-29923 Homo sapiens
pmid sentence
The tcf protein elk-1 is phosphorylated by the jnk and erk groups of mitogen-activated protein (map) kinases causing increased dna binding, ternary complex formation, and transcriptional activation
Publications: 6 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation BCL6 0.412
Identifier Residue Sequence Organism Cell Line
SIGNOR-58489 Ser333 KGLVSPQsPQKSDCQ Homo sapiens T-lymphocyte, Lymphoma Cell
pmid sentence
Here we show that antigen receptor activation leads to bcl-6 phosphorylation by mitogen-activated protein kinase (mapk). Phosphorylation, in turn, targets bcl-6 for rapid degradation by the ubiquitin/proteasome pathway.
Identifier Residue Sequence Organism Cell Line
SIGNOR-58493 Ser343 KSDCQPNsPTESCSS Homo sapiens T-lymphocyte, Lymphoma Cell
pmid sentence
Here we show that antigen receptor activation leads to bcl-6 phosphorylation by mitogen-activated protein kinase (mapk). Phosphorylation, in turn, targets bcl-6 for rapid degradation by the ubiquitin/proteasome pathway.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation PPP2R5C 0.404
Identifier Residue Sequence Organism Cell Line
SIGNOR-144317 Ser337 QLAKCVSsPHFQVAE Homo sapiens
pmid sentence
Iex-1 binds to b56 subunits and perk independently, enhances b56 phosphorylation by erk at a conserved ser/pro site in this complex and triggers dissociation from the catalytic subunit.
Publications: 1 Organism: Homo Sapiens
+ CDK1up-regulates activity img/direct-activation.png phosphorylation MAPK3 (isoform 3) 0.317
Identifier Residue Sequence Organism Cell Line
SIGNOR-277185 Ser343 PTDEVGQsPAAVGLG Homo sapiens
pmid sentence
 We found that CDK1 phosphorylates Ser343 of ERK1c, thereby allowing the binding of phosphorylated ERK1c to a complex that consists of PI4KIIIβ (also known as PI4KB) and the 14-3-3γ dimer (encoded by YWHAB). 
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation NCF1 0.427
Identifier Residue Sequence Organism Cell Line
SIGNOR-147174 Ser345 QARPGPQsPGSPLEE Homo sapiens Neutrophil
pmid sentence
Inhibitors of the erk1/2 pathway abrogated gm-csf-induced phosphorylation of ser345, while p38 mapk inhibitor abrogated tnf-alpha-induced phosphorylation of ser345.These results show that the ala-mutated p47phox acts as a dominant-negative inhibitor of endogenous p47phox and clearly indicate that phosphorylation of ser345 is required for the priming of nadph oxidase activity in neutrophil-like cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-40821 Ser345 QARPGPQsPGSPLEE Homo sapiens
pmid sentence
Upon activation, several serine residues on the cytosolic oxidase subunit p47phox become phosphorylated. Mitogen-activated protein kinase phophorylated only the peptide containing ser345/348.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation DUSP1 0.781
Identifier Residue Sequence Organism Cell Line
SIGNOR-73629 Ser359 SALSYLQsPITTSPS Homo sapiens
pmid sentence
Mkp-1 was a target in vivo and in vitro for p42(mapk) or p44(mapk), which phosphorylates mkp-1 on two carboxyl-terminal serine residues, serine 359 and serine 364. This phosphorylation did not modify mkp-1's intrinsic ability to dephosphorylate p44(mapk) but led to stabilization of the protein.
Identifier Residue Sequence Organism Cell Line
SIGNOR-73633 Ser364 LQSPITTsPSC Homo sapiens
pmid sentence
Mkp-1 was a target in vivo and in vitro for p42(mapk) or p44(mapk), which phosphorylates mkp-1 on two carboxyl-terminal serine residues, serine 359 and serine 364. This phosphorylation did not modify mkp-1's intrinsic ability to dephosphorylate p44(mapk) but led to stabilization of the protein.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation RPS6KA5 0.574
Identifier Residue Sequence Organism Cell Line
SIGNOR-131379 Ser360 TEMDPTYsPAALPQS Homo sapiens
pmid sentence
In the present study, we show that, in addition to being phosphorylated on thr-581 and ser-360 by erk1/2 or p38, msk1 can autophosphorylate on at least six sites: ser-212, ser-376, ser-381, ser-750, ser-752 and ser-758.
Identifier Residue Sequence Organism Cell Line
SIGNOR-131383 Thr581 PDNQPLKtPCFTLHY Homo sapiens
pmid sentence
In the present study, we show that, in addition to being phosphorylated on thr-581 and ser-360 by erk1/2 or p38, msk1 can autophosphorylate on at least six sites: ser-212, ser-376, ser-381, ser-750, ser-752 and ser-758.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation FOS 0.711
Identifier Residue Sequence Organism Cell Line
SIGNOR-262997 Ser362 AAAHRKGsSSNEPSS
pmid sentence
Serine 374 and serine 362 are the primary sites targeted by Erk1/2 and the mitogen-activated protein kinase-activated kinases Rsk1/2 (12, 13, 37, 38, 41), respectively. Their phosphorylation leads to protein stabilization (3, 13, 20, 41). Threonine 325 and threonine 331 are secondary targets of Erk1/2; their modification occurs only when serines 362 and 374 are phosphorylated and Erk1/2 activation is sufficiently sustained (37, 38). This enhances the transcriptional activity of c-Fos
Identifier Residue Sequence Organism Cell Line
SIGNOR-118023 Ser374 PSSDSLSsPTLLAL Homo sapiens
pmid sentence
In a previous study we have observed that exposure of nih 3t3 cells to pdgf or serum leads to c-fos phosphorylation by erk on specific residues, thr232, thr325, thr331, and ser374, within the cooh-terminal c-fos tad we have recently shown that erk phosphorylates multiple residues within the carboxylterminal transactivation domain (tad) of c-fos, thus resulting in its increased transcriptional activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-118027 Thr325 TELEPLCtPVVTCTP Homo sapiens
pmid sentence
In a previous study we have observed that exposure of nih 3t3 cells to pdgf or serum leads to c-fos phosphorylation by erk on specific residues, thr232, thr325, thr331, and ser374, within the cooh-terminal c-fos tad we have recently shown that erk phosphorylates multiple residues within the carboxylterminal transactivation domain (tad) of c-fos, thus resulting in its increased transcriptional activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-263012 Thr325 TELEPLCtPVVTCTP
pmid sentence
Serine 374 and serine 362 are the primary sites targeted by Erk1/2 and the mitogen-activated protein kinase-activated kinases Rsk1/2 (12, 13, 37, 38, 41), respectively. Their phosphorylation leads to protein stabilization (3, 13, 20, 41). Threonine 325 and threonine 331 are secondary targets of Erk1/2; their modification occurs only when serines 362 and 374 are phosphorylated and Erk1/2 activation is sufficiently sustained (37, 38). This enhances the transcriptional activity of c-Fos
Identifier Residue Sequence Organism Cell Line
SIGNOR-263008 Thr331 CTPVVTCtPSCTAYT
pmid sentence
Serine 374 and serine 362 are the primary sites targeted by Erk1/2 and the mitogen-activated protein kinase-activated kinases Rsk1/2 (12, 13, 37, 38, 41), respectively. Their phosphorylation leads to protein stabilization (3, 13, 20, 41). Threonine 325 and threonine 331 are secondary targets of Erk1/2; their modification occurs only when serines 362 and 374 are phosphorylated and Erk1/2 activation is sufficiently sustained (37, 38). This enhances the transcriptional activity of c-Fos
Identifier Residue Sequence Organism Cell Line
SIGNOR-118031 Thr331 CTPVVTCtPSCTAYT Homo sapiens
pmid sentence
In a previous study we have observed that exposure of nih 3t3 cells to pdgf or serum leads to c-fos phosphorylation by erk on specific residues, thr232, thr325, thr331, and ser374, within the cooh-terminal c-fos tad we have recently shown that erk phosphorylates multiple residues within the carboxylterminal transactivation domain (tad) of c-fos, thus resulting in its increased transcriptional activity.
Publications: 6 Organism: , Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation ASB2 0.267
Identifier Residue Sequence Organism Cell Line
SIGNOR-272241 Ser371 RIRRSGVsPLHLAAE
pmid sentence
Indeed, using mass spectrometry, we showed for the first time that ASB2a is phosphorylated and that phosphorylation of serine-323 (Ser-323) of ASB2a is crucial for the targeting of the actin-binding protein filamin A (FLNa) to degradation. |Moreover, inhibition of the extracellular signal-regulated kinases 1 and 2 (Erk1/2) activity reduced ASB2a-mediated FLNa degradation.
Publications: 1
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation RPS6KA5 0.574
Identifier Residue Sequence Organism Cell Line
SIGNOR-249479 Ser376 EKLFQGYsFVAPSIL
pmid sentence
In the present study, we show that, in addition to being phosphorylated on Thr-581 and Ser-360 by ERK1/2 or p38, MSK1 can autophosphorylate on at least six sites: Ser-212, Ser-376, Ser-381, Ser-750, Ser-752 and Ser-758. Of these sites, the N-terminal T-loop residue Ser-212 and the 'hydrophobic motif' Ser-376 are phosphorylated by the C-terminal kinase domain of MSK1, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain of MSK1
Publications: 1
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation BAG3 0.315
Identifier Residue Sequence Organism Cell Line
SIGNOR-277318 Ser377 PVPCPPPsPGPSAVP Homo sapiens HEK-293T Cell
pmid sentence
We further demonstrated BAG3, a HSP70 co-chaperone, is a bona fide substrate of SCFFBXO22. FBXO22 mediates BAG3 ubiquitination and degradation that requires ERK-dependent BAG3 phosphorylation at S377.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation GAB1 0.624
Identifier Residue Sequence Organism Cell Line
SIGNOR-249459 Ser381 CIPTAGMsPSRSNTI Cricetulus griseus CHO Cell
pmid sentence
Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. |serine and threonine phosphorylation are capable of modulating the initial signal
Identifier Residue Sequence Organism Cell Line
SIGNOR-249460 Ser454 YVPMNPNsPPRQHSS Cricetulus griseus CHO Cell
pmid sentence
Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. |serine and threonine phosphorylation are capable of modulating the initial signal
Identifier Residue Sequence Organism Cell Line
SIGNOR-249461 Ser551 ELQAPVRsPITRSFA Cricetulus griseus CHO Cell
pmid sentence
Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. |serine and threonine phosphorylation are capable of modulating the initial signal
Identifier Residue Sequence Organism Cell Line
SIGNOR-249462 Ser567 DSSRFPMsPRPDSVH Cricetulus griseus CHO Cell
pmid sentence
Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. |serine and threonine phosphorylation are capable of modulating the initial signal
Identifier Residue Sequence Organism Cell Line
SIGNOR-249463 Thr312 ISYDIPPtPGNTYQI Cricetulus griseus CHO Cell
pmid sentence
Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. |serine and threonine phosphorylation are capable of modulating the initial signal
Identifier Residue Sequence Organism Cell Line
SIGNOR-249464 Thr476 EANYVPMtPGTFDFS Cricetulus griseus CHO Cell
pmid sentence
Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. |serine and threonine phosphorylation are capable of modulating the initial signal
Publications: 6 Organism: Cricetulus Griseus
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation CASP8 0.711
Identifier Residue Sequence Organism Cell Line
SIGNOR-203480 Ser387 YLEMDLSsPQTRYIP Homo sapiens
pmid sentence
We demonstrate that perk 1/2 can phosphorylate pro-caspase-8 at s387 by knocking-down the endogenous pro-caspase-8 using rnai and replacing it with its non-phosphorylatable counterpart (s387a), a significant increase in caspase-8 activity
Publications: 1 Organism: Homo Sapiens
Tissue: Breast
+ MAPK3up-regulates img/direct-activation.png phosphorylation SPHK2 0.512
Identifier Residue Sequence Organism Cell Line
SIGNOR-153387 Ser387 PATVEPAsPTPAHSL Homo sapiens Breast Cancer Cell
pmid sentence
Sphingosine kinase type 2 activation by erk-mediated phosphorylation. site-directed mutagenesis indicated that hsphk2 is phosphorylated on ser-351 and thr-578 by erk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-153391 Thr614 AFRLEPLtPRGVLTV Homo sapiens Breast Cancer Cell
pmid sentence
Sphingosine kinase type 2 activation by erk-mediated phosphorylation. site-directed mutagenesis indicated that hsphk2 is phosphorylated on ser-351 and thr-578 by erk1
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation CTTN 0.429
Identifier Residue Sequence Organism Cell Line
SIGNOR-165208 Ser405 KTQTPPVsPAPQPTE Homo sapiens
pmid sentence
Cortactin is regulated by multiple phosphorylation events, including phosphorylation of s405 and s418 by extracellular regulated kinases (erk)1/2. Erk1/2 phosphorylation of cortactin has emerged as an important positive regulatory modification, enabling cortactin to bind and activate the arp2/3 regulator neuronal wiskott-aldrich syndrome protein (n-wasp), promoting actin polymerization and enhancing tumor cell movement.
Identifier Residue Sequence Organism Cell Line
SIGNOR-169682 Ser405 KTQTPPVsPAPQPTE Homo sapiens Neuron
pmid sentence
Cortactin is regulated by multiple phosphorylation events, including phosphorylation of s405 and s418 by extracellular regulated kinases (erk)1/2. Erk1/2 phosphorylation of cortactin has emerged as an important positive regulatory modification, enabling cortactin to bind and activate the arp2/3 regulator neuronal wiskott-aldrich syndrome protein (n-wasp), promoting actin polymerization and enhancing tumor cell movement.
Identifier Residue Sequence Organism Cell Line
SIGNOR-165212 Ser418 TEERLPSsPVYEDAA Homo sapiens
pmid sentence
Cortactin is regulated by multiple phosphorylation events, including phosphorylation of s405 and s418 by extracellular regulated kinases (erk)1/2. Erk1/2 phosphorylation of cortactin has emerged as an important positive regulatory modification, enabling cortactin to bind and activate the arp2/3 regulator neuronal wiskott-aldrich syndrome protein (n-wasp), promoting actin polymerization and enhancing tumor cell movement.
Identifier Residue Sequence Organism Cell Line
SIGNOR-169686 Ser418 TEERLPSsPVYEDAA Homo sapiens Neuron
pmid sentence
Cortactin is regulated by multiple phosphorylation events, including phosphorylation of s405 and s418 by extracellular regulated kinases (erk)1/2. Erk1/2 phosphorylation of cortactin has emerged as an important positive regulatory modification, enabling cortactin to bind and activate the arp2/3 regulator neuronal wiskott-aldrich syndrome protein (n-wasp), promoting actin polymerization and enhancing tumor cell movement.
Publications: 4 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation ARRB1 0.709
Identifier Residue Sequence Organism Cell Line
SIGNOR-67634 Ser412 EEEDGTGsPQLNNR Homo sapiens
pmid sentence
Erk1 and erk2 phosphorylate beta-arrestin1 at ser-412 in vitro. . in the resting state, cytosolic arrestin1 proteins are constitutively phosphorylated by extracellular signal-regulated kinase (erk) at ser412, located within their distal c terminus. erk-phosphorylated arrestin1 is unable to associate with clathrin cages, whereas this constraint is removed upon its dephosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-129589 Ser412 EEEDGTGsPQLNNR Homo sapiens
pmid sentence
Erk1 and erk2 phosphorylate beta-arrestin1 at ser-412 in vitro. . in the resting state, cytosolic arrestin1 proteins are constitutively phosphorylated by extracellular signal-regulated kinase (erk) at ser412, located within their distal c terminus. erk-phosphorylated arrestin1 is unable to associate with clathrin cages, whereas this constraint is removed upon its dephosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-183484 Ser412 EEEDGTGsPQLNNR Homo sapiens
pmid sentence
Erk1 and erk2 phosphorylate beta-arrestin1 at ser-412 in vitro. . in the resting state, cytosolic arrestin1 proteins are constitutively phosphorylated by extracellular signal-regulated kinase (erk) at ser412, located within their distal c terminus. erk-phosphorylated arrestin1 is unable to associate with clathrin cages, whereas this constraint is removed upon its dephosphorylation
Publications: 3 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation XPO5 0.315
Identifier Residue Sequence Organism Cell Line
SIGNOR-262979 Ser416 GFPSKTDsPSCEYSR Homo sapiens HEK-293 Cell
pmid sentence
Here we show that ERK suppresses pre-miRNA export from the nucleus through phosphorylation of exportin-5 (XPO5) at T345/S416/S497. After phosphorylation by ERK, conformation of XPO5 is altered by prolyl isomerase Pin1, resulting in reduction of pre-miRNA loading. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-262982 Ser497 GSLCSVFsPSFVQWE Homo sapiens
pmid sentence
Here we show that ERK suppresses pre-miRNA export from the nucleus through phosphorylation of exportin-5 (XPO5) at T345/S416/S497. After phosphorylation by ERK, conformation of XPO5 is altered by prolyl isomerase Pin1, resulting in reduction of pre-miRNA loading. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-262985 Thr345 GADSDVEtPSNFGKY Homo sapiens
pmid sentence
Here we show that ERK suppresses pre-miRNA export from the nucleus through phosphorylation of exportin-5 (XPO5) at T345/S416/S497. After phosphorylation by ERK, conformation of XPO5 is altered by prolyl isomerase Pin1, resulting in reduction of pre-miRNA loading. 
Publications: 3 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation GRB10 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-249406 Ser418 QQRKALLsPFSTPVR in vitro
pmid sentence
We identified Ser150, Ser418, and Ser476 of human Grb10 as MAPK-mediated in vitro phosphorylation sites.
Publications: 1 Organism: In Vitro
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation STK11 0.395
Identifier Residue Sequence Organism Cell Line
SIGNOR-209880 Ser428 SSKIRRLsACKQQ Homo sapiens
pmid sentence
Directly and/or through the activation of p90RSK, ERK phosphorylates LKB-1 at Ser325 and Ser428. The phosphorylation of LKB-1 causes the dissociation of LKB-1 from AMPK, resulting in the impaired activation of AMPK.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates quantity by stabilization img/direct-activation.png phosphorylation METTL3 0.273
Identifier Residue Sequence Organism Cell Line
SIGNOR-265949 Ser43 RNPEAALsPTFRSDS Homo sapiens HEK-293 Cell
pmid sentence
Mass spectrometry analysis showed that ERK phosphorylates METTL3 at three highly conserved residues: S43, S50, and S525 (Figures 2D and 2E). Mutational analysis further confirmed these three sites as main ERK phosphorylation sites (Figure 2F). Phosphorylation of METTL3 increases interaction with USP5, decreasing ubiquitination to stabilize the m6 A methyltransferase complex.
Identifier Residue Sequence Organism Cell Line
SIGNOR-265945 Ser50 SPTFRSDsPVPTAPT Homo sapiens HEK-293 Cell
pmid sentence
Mass spectrometry analysis showed that ERK phosphorylates METTL3 at three highly conserved residues: S43, S50, and S525 (Figures 2D and 2E). Mutational analysis further confirmed these three sites as main ERK phosphorylation sites (Figure 2F). Phosphorylation of METTL3 increases interaction with USP5, decreasing ubiquitination to stabilize the m6 A methyltransferase complex.
Identifier Residue Sequence Organism Cell Line
SIGNOR-265947 Ser525 YGMIERLsPGTRKIE Homo sapiens HEK-293 Cell
pmid sentence
Mass spectrometry analysis showed that ERK phosphorylates METTL3 at three highly conserved residues: S43, S50, and S525 (Figures 2D and 2E). Mutational analysis further confirmed these three sites as main ERK phosphorylation sites (Figure 2F). Phosphorylation of METTL3 increases interaction with USP5, decreasing ubiquitination to stabilize the m6 A methyltransferase complex.
Publications: 3 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation KRT8 0.425
Identifier Residue Sequence Organism Cell Line
SIGNOR-249468 Ser432 SAYGGLTsPGLSYSL
pmid sentence
Also, several probable in vivo K8 kinases have been identified including Erk1/2 for K8 Ser431 (Ku and Omary, 1997), and p38 and Jun kinases for K8 Ser73 (Ku et al., 2002a; He et al., 2002).
Identifier Residue Sequence Organism Cell Line
SIGNOR-196141 Ser432 SAYGGLTsPGLSYSL Homo sapiens
pmid sentence
Our data suggested a close relationship between k8(s431) phosphorylation and keratin reorganization in epithelial tumor cells.
Publications: 2 Organism: , Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation SREBF2 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-123049 Ser432 NQNVLLMsPPASDSG Homo sapiens
pmid sentence
Insulin-activated erk-mitogen-activated protein kinases phosphorylate sterol regulatory element-binding protein-2 at serine residues 432 and 455 in vivo.Further characterization by electrophoretic mobility shift assay and promoter reporter gene analyses revealed that phosphorylation does not influence protein/dna interaction, but enhances trans-activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-123053 Ser455 SIDSEPGsPLLDDAK Homo sapiens
pmid sentence
Insulin-activated erk-mitogen-activated protein kinases phosphorylate sterol regulatory element-binding protein-2 at serine residues 432 and 455 in vivo.Further characterization by electrophoretic mobility shift assay and promoter reporter gene analyses revealed that phosphorylation does not influence protein/dna interaction, but enhances trans-activity.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation RPS6KB1 0.597
Identifier Residue Sequence Organism Cell Line
SIGNOR-111515 Ser441 SPRRFIGsPRTPVSP Rattus norvegicus
pmid sentence
Thr 421/Ser 424 have been reported to be targeted by ERK1, 2 (39), JNK or p38 MAPKs (36). Interestingly, with a comparable kinetics, FSH represses ERK1, 2 constitutive phosphorylation in Sertoli cells isolated from 19-d-old rats
Identifier Residue Sequence Organism Cell Line
SIGNOR-134662 Thr444 RFIGSPRtPVSPVKF Rattus norvegicus
pmid sentence
Thr 421/Ser 424 have been reported to be targeted by ERK1, 2 (39), JNK or p38 MAPKs (36). Interestingly, with a comparable kinetics, FSH represses ERK1, 2 constitutive phosphorylation in Sertoli cells isolated from 19-d-old rats
Identifier Residue Sequence Organism Cell Line
SIGNOR-121997 Homo sapiens
pmid sentence
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase
Publications: 3 Organism: Rattus Norvegicus, Homo Sapiens
Tissue: Carcinoma Cell
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation MRTFA 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-179963 Ser454 TGSTPPVsPTPSERS Homo sapiens
pmid sentence
Serum induces rhoa-dependent translocation of mkl1 from the cytoplasm to the nucleus and also causes a rapid increase in mkl1 phosphorylation. Serum-induced phosphorylation of the serum response factor coactivator mkl1 by the extracellular signal-regulated kinase 1/2 pathway inhibits its nuclear localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-195157 Ser454 TGSTPPVsPTPSERS Homo sapiens Breast Cancer Cell, Lung Cancer Cell
pmid sentence
Serum induces rhoa-dependent translocation of mkl1 from the cytoplasm to the nucleus and also causes a rapid increase in mkl1 phosphorylation. Serum-induced phosphorylation of the serum response factor coactivator mkl1 by the extracellular signal-regulated kinase 1/2 pathway inhibits its nuclear localization.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation KLC1 0.266
Identifier Residue Sequence Organism Cell Line
SIGNOR-172642 Ser460 YKACKVDsPTVTTTL Homo sapiens
pmid sentence
Phosphorylation of kinesin light chain 1 at serine 460 modulates binding and trafficking of calsyntenin-1mutation of klc1ser460 to an alanine residue, to preclude phosphorylation, increased the binding of calsyntenin-1, whereas mutation to an aspartate residueklc1ser460 is a predicted mitogen-activated protein kinase (mapk) target site, and we show that extracellular-signal-regulated kinase (erk) phosphorylates this residue in vitro.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation SYN3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-121402 Ser470 PQGQQPLsPQSGSPQ Homo sapiens
pmid sentence
A rare, missense polymorphism, s470n, was identified in the synapsin iii gene and appeared more frequently in individuals with schizophrenia than in controls. Ser470, was determined to be a substrate for mitogen-activated protein kinase, a downstream effector of neurotrophin action.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates quantity by stabilization img/direct-activation.png phosphorylation EPAS1 0.262
Identifier Residue Sequence Organism Cell Line
SIGNOR-277584 Ser484 SSCSTPNsPEDYYTS Homo sapiens Glioma Stem Cell
pmid sentence
The activation of ERK1/2 upon hypoxia promoted HIF-2alpha phosphorylation, enhancing its interaction with USP33.Here, we identified USP33 as essential deubiquitinase that stabilizes HIF-2alpha protein in an ERK1/2-dependent manner to promote hypoxia response in cancer cells.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation CAPN2 0.554
Identifier Residue Sequence Organism Cell Line
SIGNOR-123083 Ser50 GTLFQDPsFPAIPSA Homo sapiens
pmid sentence
Epidermal growth factor activates m-calpain (calpain ii), at least in part, by extracellular signal-regulated kinase-mediated phosphorylation.We now show that erk directly phosphorylates and activates m-calpain both in vitro and in vivo. We identified serine 50 as required for epidermal growth factor (egf)-induced calpain activation in vitro and in vivo.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation PLA2G4A 0.65
Identifier Residue Sequence Organism Cell Line
SIGNOR-38434 Ser505 LNTSYPLsPLSDFAT Homo sapiens
pmid sentence
Activated map kinase phosphorylates cpla2 at ser-505, causing increased enzymatic activity of cpla2, which is only realized upon translocation of cpla2 to the membrane.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation NANOG 0.501
Identifier Residue Sequence Organism Cell Line
SIGNOR-278487 Ser52 MPHTETVsPLPSSMD Homo sapiens
pmid sentence
We found that activation of ERK1 signaling inhibited transactivation activity of Nanog.|We showed the direct phosphorylation of Nanog by ERK1 and clearly showed that Ser52 is the major phosphorylation site and Ser65 is weakly phosphorylated by ERK1.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation JAK2 0.51
Identifier Residue Sequence Organism Cell Line
SIGNOR-146747 Ser523 GVSDVPTsPTLQRPT Homo sapiens
pmid sentence
We hypothesize that phosphorylation of ser523 in jak2 by erks 1 and/or 2 or other as-yet-unidentified kinases acts in a negative feedback manner
Publications: 1 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation NUP153 0.396
Identifier Residue Sequence Organism Cell Line
SIGNOR-188143 Ser529 SPMFKFSsPIVKSTE Homo sapiens
pmid sentence
These results indicate that phosphorylation of nup153 and nup214 by erk strongly reduces their affinity for importin-. nup153 depletion caused a strong inhibition of nuclear accumulation of gfp?importin-beta in both erk-inhibited and erk-activated cells (fig. 8b,c), indicating that nup153 is essential for the efficient importin-beta transport.
Identifier Residue Sequence Organism Cell Line
SIGNOR-188147 Thr388 VYFKPSLtPSGEFRK Homo sapiens
pmid sentence
These results indicate that phosphorylation of nup153 and nup214 by erk strongly reduces their affinity for importin-. nup153 depletion caused a strong inhibition of nuclear accumulation of gfp?importin-beta in both erk-inhibited and erk-activated cells (fig. 8b,c), indicating that nup153 is essential for the efficient importin-beta transport.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation PML 0.343
Identifier Residue Sequence Organism Cell Line
SIGNOR-124317 Ser530 DGPPSPRsPVIGSEV Homo sapiens Leukemia Cell
pmid sentence
Phosphorylation of pml by mitogen-activated protein kinases plays a key role in arsenic trioxide-mediated apoptosis.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation MYB 0.304
Identifier Residue Sequence Organism Cell Line
SIGNOR-45348 Ser532 KIKQEVEsPTDKSGN Homo sapiens JURKAT Cell
pmid sentence
Functional analysis of phosphorylation at serine 532 of human c-myb by map kinase. expression of a polypeptide containing the c-myb c-terminal domain stimulated c-myb activity. This effect is reduced upon mapk-dependent phosphorylation of serine 532. Our data suggest that the mapk-dependent state of phosphorylation modifies the cellular function of c-myb by modulating its interaction with a putative inhibitory factor
Identifier Residue Sequence Organism Cell Line
SIGNOR-43558 Ser532 KIKQEVEsPTDKSGN Homo sapiens
pmid sentence
Here we describe that human c-myb can be phosphorylated by mitogen-activated protein kinases (mapk's) at serine 532 of the carboxy (c-) terminal regulatory domain in vitro. expression of a constitutively active form of ras together with c-myb in transient transfection experiments had no effect on the transcriptional activity of c-myb, while expression of a polypeptide containing the c-myb c-terminal domain stimulated c-myb activity. This effect is reduced upon mapk-dependent phosphorylation of serine 532.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation TSC2 0.687
Identifier Residue Sequence Organism Cell Line
SIGNOR-249458 Ser540 KVMARSLsPPPELEE Mus musculus
pmid sentence
Here, we show that Erk may play a critical role in TSC progression through posttranslational inactivation of TSC2. Erk-dependent phosphorylation leads to TSC1-TSC2 dissociation and markedly impairs TSC2 ability to inhibit mTOR signaling, cell proliferation, and oncogenic transformation. |Serine to alanine substitution at S664 or double S664A/S540A mutagenesis resulted in a marked reduction in TSC2 phosphorylation to a similar extent. In contrast, S540A substitution only moderately impaired TSC2 phosphorylation (Figure 3D), corroborating the notion that in vivo S664 is the most relevant residue for Erk-mediated phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249457 Ser664 KKTSGPLsPPTGPPG Mus musculus NIH-3T3 Cell
pmid sentence
Here, we show that Erk may play a critical role in TSC progression through posttranslational inactivation of TSC2. Erk-dependent phosphorylation leads to TSC1-TSC2 dissociation and markedly impairs TSC2 ability to inhibit mTOR signaling, cell proliferation, and oncogenic transformation. |Serine to alanine substitution at S664 or double S664A/S540A mutagenesis resulted in a marked reduction in TSC2 phosphorylation to a similar extent. In contrast, S540A substitution only moderately impaired TSC2 phosphorylation (Figure 3D), corroborating the notion that in vivo S664 is the most relevant residue for Erk-mediated phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-183695 Homo sapiens
pmid sentence
Phosphorylation of tsc2 (by akt and erk;refs. 28, 29) and tsc1(by ikkbeta;ref. 30) results in the disruption of the tsc1/2 complex, and thereby activates the oncogenic mtor signaling contributing to tumor progression
Publications: 3 Organism: Mus Musculus, Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation WWC1 0.271
Identifier Residue Sequence Organism Cell Line
SIGNOR-203290 Ser548 SSPSPPCsPLMADPL Homo sapiens
pmid sentence
We demonstrated that erk1/2 phosphorylate kibra at ser(548) in cells as well as in vitro.
Publications: 1 Organism: Homo Sapiens
Tissue: Breast
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation LCK 0.554
Identifier Residue Sequence Organism Cell Line
SIGNOR-249469 Ser59 EGSNPPAsPLQDNLV Homo sapiens HeLa Cell
pmid sentence
Phosphorylation at Ser-59 (or alternatively, its mutation to Glu) reverses the inhibition and allows interaction of the p56lck SH2 domain with p62.|phosphotyrosine-independent binding of p62 to the p56lck SH2 domain appears to provide an alternative pathway for p56lck signaling that is regulated by Ser-59 phosphorylation.
Publications: 1 Organism: Homo Sapiens
Pathways:Glucocorticoid receptor Signaling
+ MAPK3up-regulates img/direct-activation.png phosphorylation SP1 0.644
Identifier Residue Sequence Organism Cell Line
SIGNOR-248079 Ser59 GGQESQPsPLALLAA Homo sapiens
pmid sentence
PKCalpha, which was activated in senescent cells by ROS strongly activated Erk1/2, and the SA-pErk1/2 in turn phosphorylated Sp1 on Ser(59). Sp1-enhanced transcription of p21(Sdi1) resulted in regulation of cellular senescence in primary human diploid fibroblast cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248066 Thr453 SGPIIIRtPTVGPNG Homo sapiens
pmid sentence
We showed that perifosine activates the mitogen-activated protein/extracellular signal-regulated kinase pathway, and this activation promotes the phosphorylation of sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased sp1 binding and enhanced p21(waf1/cip1) transcription.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248062 Thr453 SGPIIIRtPTVGPNG Homo sapiens HaCaT Cell
pmid sentence
We showed that perifosine activates the mitogen-activated protein/extracellular signal-regulated kinase pathway, and this activation promotes the phosphorylation of sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased sp1 binding and enhanced p21(waf1/cip1) transcription.
Identifier Residue Sequence Organism Cell Line
SIGNOR-118936 Thr739 SEGSGTAtPSALITT Homo sapiens
pmid sentence
We showed that perifosine activates the mitogen-activated protein/extracellular signal-regulated kinase pathway, and this activation promotes the phosphorylation of sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased sp1 binding and enhanced p21(waf1/cip1) transcription.
Identifier Residue Sequence Organism Cell Line
SIGNOR-116174 Thr739 SEGSGTAtPSALITT Homo sapiens
pmid sentence
Here we show that p42/p44 mapk directly phosphorylates sp1 on threonines 453 and 739 both in vitro and in vivo. sa-perk1/2 activates the transcription factor, sp1, via ser59 phosphorylation downstream of pkc_, leading to transcription of p21sdi1 and resulting in replicative senescence of hdf cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-248070 Thr739 SEGSGTAtPSALITT Homo sapiens HaCaT Cell
pmid sentence
We showed that perifosine activates the mitogen-activated protein/extracellular signal-regulated kinase pathway, and this activation promotes the phosphorylation of sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased sp1 binding and enhanced p21(waf1/cip1) transcription.
Publications: 6 Organism: Homo Sapiens
Tissue: Muscle, Smooth Muscle
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation IRS1 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-123177 Ser616 DDGYMPMsPGVAPVP Homo sapiens
pmid sentence
Rin beta-cells exposed to high glucose exhibited increased c-jun n-terminal kinase (jnk) and erk1/2 activity, which was associated with increased irs-1 phosphorylation at serine (ser)(307) and ser(612), respectively, that inhibits coupling of irs-1 to the insulin receptor and is upstream of the inhibition of irs-1 tyrosine phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249409 Ser636 SGDYMPMsPKSVSAP Homo sapiens
pmid sentence
Insulin also activates jnk, erk, pkc and mtor, which induce the phosphorylation of irs1 on serine residues 307, 612 and 632 and inhibit its functions. Our results indicate that the insulin-stimulated degradation of irs-1 via the phosphatidylinositol 3-kinase pathway is in part dependent upon the ser(312) phosphorylation of irs-1.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation MYC 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-236250 Ser62 LLPTPPLsPSRRSGL in vitro
pmid sentence
ERK1 phosphorylates MYC Ser62 resulting in MYC stabilization and activation
Publications: 1 Organism: In Vitro
+ MAPK3up-regulates img/direct-activation.png phosphorylation TH 0.49
Identifier Residue Sequence Organism Cell Line
SIGNOR-34678 Ser62 SYTPTPRsPRFIGRR Homo sapiens
pmid sentence
In this paper we have studied the phosphorylation and activation of alternatively spliced forms of human th by mapkap kinase-1 , mapkap kinase-2, map kinase, and cam kinase-11
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation STMN2 0.356
Identifier Residue Sequence Organism Cell Line
SIGNOR-249115 Ser62 ELILKPPsPISEAPR Rattus norvegicus Brain
pmid sentence
SCG10, a growth cone-enriched MT-destabilizing protein, has been recently characterized as an in vitro substrate for various serine/threonine kinases including PKA, MAP kinase, and CDK (19). We have found that SCG10 is phosphorylated in vivo in developing rat brain.| The sites for MAP kinase phosphorylation were identified as Ser-62 and Ser-73 of SCG10|By expressing a series of phosphorylation site mutants, we showed that the MT-destabilizing effect of SCG10 could be modulated. While the nonphosphorylatable mutant showed higher activity than the wild-type protein, the activity of the mutant in which phosphorylation on all four sites was mimicked by an aspartate residue was greatly reduced. These data suggest that the nonphosphorylated state of SCG10 represents the most active form of the protein.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249116 Ser73 EAPRTLAsPKKKDLS Rattus norvegicus Brain
pmid sentence
SCG10, a growth cone-enriched MT-destabilizing protein, has been recently characterized as an in vitro substrate for various serine/threonine kinases including PKA, MAP kinase, and CDK (19). We have found that SCG10 is phosphorylated in vivo in developing rat brain.| The sites for MAP kinase phosphorylation were identified as Ser-62 and Ser-73 of SCG10|By expressing a series of phosphorylation site mutants, we showed that the MT-destabilizing effect of SCG10 could be modulated. While the nonphosphorylatable mutant showed higher activity than the wild-type protein, the activity of the mutant in which phosphorylation on all four sites was mimicked by an aspartate residue was greatly reduced. These data suggest that the nonphosphorylated state of SCG10 represents the most active form of the protein.
Publications: 2 Organism: Rattus Norvegicus
+ MAPK3up-regulates img/direct-activation.png phosphorylation GAB2 0.606
Identifier Residue Sequence Organism Cell Line
SIGNOR-128731 Ser623 ALDFQPSsPSPHRKP Homo sapiens
pmid sentence
Phosphorylation of grb2-associated binder 2 on serine 623 by erk mapk regulates its association with the phosphatase shp-2 and decreases stat5 activation.We and others have demonstrated that il-2-induced tyrosine phosphorylation of gab2 and its interaction with its sh2 domain-containing partners, shp-2, p85 pi3k, and crkl (5, 26, 27). we report that pretreatment of kit 225 cells with the mek inhibitor u0126, strongly decreased the characteristic shift of gab2 in response to il-2 and increased gab2/shp-2 association, an effect that could be ascribed to erk phosphorylation of serine 623.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation EPS8 0.327
Identifier Residue Sequence Organism Cell Line
SIGNOR-263058 Ser625 ADTPPAPsPPPTPAP in vitro
pmid sentence
We further show that the actin barbed-end capping activity of Eps8 is inhibited by brain-derived neurotrophic factor (BDNF) treatment through MAPK-dependent phosphorylation of Eps8 residues S624 and T628. Additionally, an Eps8 mutant, impaired in the MAPK target sites (S624A/T628A), displays increased association to actin-rich structures, is resistant to BDNF-mediated release from microfilaments, and inhibits BDNF-induced filopodia. The opposite is observed for a phosphomimetic Eps8 (S624E/T628E) mutant. Thus, collectively, our data identify Eps8 as a critical capping protein in the regulation of axonal filopodia and delineate a molecular pathway by which BDNF, through MAPK-dependent phosphorylation of Eps8, stimulates axonal filopodia formation, a process with crucial impacts on neuronal development and synapse formation.
Publications: 1 Organism: In Vitro
+ MAPK3up-regulates img/direct-activation.png phosphorylation JUN 0.777
Identifier Residue Sequence Organism Cell Line
SIGNOR-91379 Ser63 KNSDLLTsPDVGLLK Homo sapiens
pmid sentence
Up-regulation of c-jun mrna in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-jun n-terminal kinases are required for efficient up-regulation of c-jun protein.
Identifier Residue Sequence Organism Cell Line
SIGNOR-91383 Ser73 VGLLKLAsPELERLI Homo sapiens
pmid sentence
Up-regulation of c-jun mrna in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-jun n-terminal kinases are required for efficient up-regulation of c-jun protein.
Publications: 2 Organism: Homo Sapiens
Pathways:Glucocorticoid receptor Signaling
+ MAPK3up-regulates img/direct-activation.png phosphorylation RRN3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-98980 Ser633 SFDTHFRsPSSSVGS Homo sapiens
pmid sentence
Erk-dependent phosphorylation of the transcription initiation factor tif-ia is required for rna polymerase i transcription and cell growth. phosphopeptide mapping and mutational analysis reveals two serine residues (s633 and s649) that are phosphorylated by erk and rsk kinases. Replacement of s649 by alanine inactivates tif-ia, inhibits pre-rrna synthesis, and retards cell growth.
Identifier Residue Sequence Organism Cell Line
SIGNOR-98984 Ser649 PVLYMQPsPL Homo sapiens
pmid sentence
Erk-dependent phosphorylation of the transcription initiation factor tif-ia is required for rna polymerase i transcription and cell growth. phosphopeptide mapping and mutational analysis reveals two serine residues (s633 and s649) that are phosphorylated by erk and rsk kinases. Replacement of s649 by alanine inactivates tif-ia, inhibits pre-rrna synthesis, and retards cell growth.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation HIF1A 0.691
Identifier Residue Sequence Organism Cell Line
SIGNOR-178731 Ser641 DIKILIAsPSPTHIH Homo sapiens HeLa Cell
pmid sentence
We show that at least two different nuclear protein kinases, one of them identified as p42/p44 mapk, can modify hif-1_. Analysis of in vitro phosphorylated hif-1_ by mass spectroscopy revealed residues ser-641 and ser-643 as possible mapk phosphorylation sites these data suggest that phosphorylation of ser-641/643 by mapk promotes the nuclear accumulation and transcriptional activity of hif-1_
Identifier Residue Sequence Organism Cell Line
SIGNOR-178735 Ser643 KILIASPsPTHIHKE Homo sapiens HeLa Cell
pmid sentence
We show that at least two different nuclear protein kinases, one of them identified as p42/p44 mapk, can modify hif-1_. Analysis of in vitro phosphorylated hif-1_ by mass spectroscopy revealed residues ser-641 and ser-643 as possible mapk phosphorylation sites these data suggest that phosphorylation of ser-641/643 by mapk promotes the nuclear accumulation and transcriptional activity of hif-1_
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation GTF2I 0.379
Identifier Residue Sequence Organism Cell Line
SIGNOR-74304 Ser668 INTKALQsPKRPRSP Homo sapiens
pmid sentence
Tfii-i can be phosphorylated in vitro by erk and mutation of consensus map kinase substrate sites at serines 627 and 633 impairs the phosphorylation of tfii-i by erk and its activity on the c-fos promoter. These results suggest that erk regulates the activity of tfii-i by direct phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-74308 Ser674 QSPKRPRsPGSNSKV Homo sapiens
pmid sentence
Tfii-i can be phosphorylated in vitro by erk and mutation of consensus map kinase substrate sites at serines 627 and 633 impairs the phosphorylation of tfii-i by erk and its activity on the c-fos promoter. These results suggest that erk regulates the activity of tfii-i by direct phosphorylation.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation GRK2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-72582 Ser670 KMKNKPRsPVVELSK Homo sapiens HEK-293 Cell
pmid sentence
Erk1 phosphorylates grk2 at ser(670). Inhibition of erk activity in hek293 cells potentiates grk2 activity, whereas, conversely, erk activation inhibits grk2 activity.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation NFATC4 0.292
Identifier Residue Sequence Organism Cell Line
SIGNOR-133276 Ser676 SNGRRKRsPTQSFRF Homo sapiens
pmid sentence
The formation of rsk-nfatc4-dna transcription complex is also apparent upon adipogenesis. Bound rsk phosphorylates ser(676) and potentiates nfatc4 dna binding by escalating nfat-dna association. Ser(676) is also targeted by the erk map kinase, which interacts with nfat at a distinct region than rsk. Thus, integration of the erk/rsk signaling pathway provides a mechanism to modulate nfatc4 transcription activity.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation TWIST1 0.332
Identifier Residue Sequence Organism Cell Line
SIGNOR-173413 Ser68 GGGDEPGsPAQGKRG Homo sapiens Breast Cancer Cell
pmid sentence
Phosphorylation of serine 68 of twist1 by mapks stabilizes twist1 protein and promotes breast cancer cell invasiveness. this ser 68 is phosphorylated by p38, c-jun n-terminal kinases (jnk), and extracellular signal-regulated kinases1/2 in vitro
Publications: 1 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation ARHGAP26 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262945 Ser685 PMFSAPSsPMPTSST in vitro
pmid sentence
In vitro, purified mitogen-activated protein (MAP) kinase catalyzed the phosphorylation of Graf on serine 510, suggesting that Graf phosphorylation may be mediated through MAP kinase signaling.
Publications: 1 Organism: In Vitro
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation BCL2L11 0.725
Identifier Residue Sequence Organism Cell Line
SIGNOR-129878 Ser69 GPLAPPAsPGPFATR Homo sapiens
pmid sentence
In vitro, bimel was phosphorylated by extracellular signal-regulated kinase on ser(69), which resides in the bimel-specific insert region. Using phosphospecific antibody against this site, we show that this residue is actually phosphorylated in cells. We also show that phosphorylation of ser(69) promotes ubiquitination of bimel. We conclude that mek inhibitors sensitize mda-mb231 and hbc4 cells to anoikis by blocking phosphorylation and hence degradation of bimel
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation RPTOR 0.477
Identifier Residue Sequence Organism Cell Line
SIGNOR-169526 Ser696 EKNYALPsPATTEGG Homo sapiens HEK-293 Cell
pmid sentence
We found three proline-directed residues within raptor, ser(8), ser(696), and ser(863), which are directly phosphorylated by erk1/2. Expression of phosphorylation-deficient alleles of raptor revealed that phosphorylation of these sites by erk1/2 normally promotes mtorc1 activity and signaling to downstream substrates, such as 4e-bp1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-169530 Ser8 MESEMLQsPLLGLGE Homo sapiens HEK-293 Cell
pmid sentence
We found three proline-directed residues within raptor, ser(8), ser(696), and ser(863), which are directly phosphorylated by erk1/2. Expression of phosphorylation-deficient alleles of raptor revealed that phosphorylation of these sites by erk1/2 normally promotes mtorc1 activity and signaling to downstream substrates, such as 4e-bp1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-169534 Ser863 LTQSAPAsPTNKGVH Homo sapiens
pmid sentence
We found three proline-directed residues within raptor, ser(8), ser(696), and ser(863), which are directly phosphorylated by erk1/2. Expression of phosphorylation-deficient alleles of raptor revealed that phosphorylation of these sites by erk1/2 normally promotes mtorc1 activity and signaling to downstream substrates, such as 4e-bp1.
Publications: 3 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation BCL2 0.548
Identifier Residue Sequence Organism Cell Line
SIGNOR-74935 Ser70 RDPVARTsPLQTPAA Homo sapiens
pmid sentence
Erk1 and erk2 directly phosphorylate bcl2 exclusively at ser-70 p44mapk/extracellular signal-regulated kinase 1 (erk1) and p42 mapk/erk2 are activated by il-3, colocalize with mitochondrial bcl2, and can directly phosphorylate bcl2 on ser-70 in a stauro-resistant manner both in vitro and in vivo molecular association.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation PDE4D 0.254
Identifier Residue Sequence Organism Cell Line
SIGNOR-77578 Ser715 YQSTIPQsPSPAPDD Homo sapiens
pmid sentence
These straddle the target residue, ser(579), for erk2 phosphorylation of pde4d3. Mutation of either or both of these docking sites prevented erk2 from being co-immunoprecipitated with pde4d3, ablated the ability of epidermal growth factor to inhibit pde4d3 through erk2 action in transfected cos cells, and attenuated the ability of erk2 to phosphorylate pde4d3 in vitro.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation STAT3 0.713
Identifier Residue Sequence Organism Cell Line
SIGNOR-187779 Ser727 NTIDLPMsPRTLDSL Homo sapiens
pmid sentence
The activation of stat-3 is regulated by phosphorylation of tyrosine 705 by receptor and nonreceptor protein tyrosine kinases these include epidermal growth factor receptor (egfr) kinase,92 src,5 janus-activated kinases (jak), and extracellular signal-regulated kinase (erk)a constitutively active galpha16 mutant, galpha16ql, stimulated stat3-dependent luciferase activity as well as the phosphorylation of stat3 at both tyr705 and ser727. Galpha16ql-induced stat3 activation was enhanced by overexpression of extracellular signal-regulated kinase 1 (erk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-118596 Ser727 NTIDLPMsPRTLDSL Homo sapiens Leukemia Cell
pmid sentence
The activation of stat-3 is regulated by phosphorylation of tyrosine 705 by receptor and nonreceptor protein tyrosine kinases these include epidermal growth factor receptor (egfr) kinase,92 src,5 janus-activated kinases (jak), and extracellular signal-regulated kinase (erk)a constitutively active galpha16 mutant, galpha16ql, stimulated stat3-dependent luciferase activity as well as the phosphorylation of stat3 at both tyr705 and ser727. Galpha16ql-induced stat3 activation was enhanced by overexpression of extracellular signal-regulated kinase 1 (erk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-187787 Tyr705 DPGSAAPyLKTKFIC Homo sapiens
pmid sentence
The activation of stat-3 is regulated by phosphorylation of tyrosine 705 by receptor and nonreceptor protein tyrosine kinases these include epidermal growth factor receptor (egfr) kinase,92 src,5 janus-activated kinases (jak), and extracellular signal-regulated kinase (erk)a constitutively active galpha16 mutant, galpha16ql, stimulated stat3-dependent luciferase activity as well as the phosphorylation of stat3 at both tyr705 and ser727. Galpha16ql-induced stat3 activation was enhanced by overexpression of extracellular signal-regulated kinase 1 (erk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-118600 Tyr705 DPGSAAPyLKTKFIC Homo sapiens Leukemia Cell
pmid sentence
The activation of stat-3 is regulated by phosphorylation of tyrosine 705 by receptor and nonreceptor protein tyrosine kinases these include epidermal growth factor receptor (egfr) kinase,92 src,5 janus-activated kinases (jak), and extracellular signal-regulated kinase (erk)a constitutively active galpha16 mutant, galpha16ql, stimulated stat3-dependent luciferase activity as well as the phosphorylation of stat3 at both tyr705 and ser727. Galpha16ql-induced stat3 activation was enhanced by overexpression of extracellular signal-regulated kinase 1 (erk1
Publications: 4 Organism: Homo Sapiens
Tissue: Kidney
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation STAT3 0.713
Identifier Residue Sequence Organism Cell Line
SIGNOR-249450 Ser727 NTIDLPMsPRTLDSL Homo sapiens HEK-293 Cell
pmid sentence
The hematopoietic-specific Galpha16 protein has recently been shown to mediate receptor-induced activation of the signal transducer and activator of transcription 3 (STAT3). In the present study, we have delineated the mechanism by which Galpha16 stimulates STAT3 in human embryonic kidney 293 cells. A constitutively active Galpha16 mutant, Galpha16QL, stimulated STAT3-dependent luciferase activity as well as the phosphorylation of STAT3 at both Tyr705 and Ser727. Galpha16QL-induced STAT3 activation was enhanced by overexpression of extracellular signal-regulated kinase 1 (ERK1),
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation SP3 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-157276 Ser73 CSKIGPPsPGDDEEE Homo sapiens
pmid sentence
Here, we show that sp3, which, as sp1, belongs to the gc-rich binding transcription factor family, is also phosphorylated by erk in vitro on serine 73.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation NOS2 0.359
Identifier Residue Sequence Organism Cell Line
SIGNOR-157711 Ser745 KSRQNLQsPTSSRAT Homo sapiens HEK-293 Cell
pmid sentence
Erk phosphorylated inos on ser745. Mutation of ser745 to ala did not affect basal inos activity but eliminated inos phosphorylation and activation in response to b1r agonist.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation CALD1 0.467
Identifier Residue Sequence Organism Cell Line
SIGNOR-71041 Ser759 KTPDGNKsPAPKPSD Homo sapiens
pmid sentence
Extracellular signal-regulated kinases (erks) phosphorylate the high molecular mass isoform of the actin-binding protein caldesmon (h-cad) at two sites (ser(759) and ser(789)) during smooth muscle stimulation. Nmr spectroscopy shows that the actin binding properties of the minimal inhibitory region of caldesmon, residues 750-779, alter upon map kinase phosphorylation of ser-759, a residue not involved in actin binding. This phosphorylation leads to markedly diminished actin affinity as a result of the loss of interaction at one of the two sites that bind to f-actin.
Identifier Residue Sequence Organism Cell Line
SIGNOR-86741 Ser759 KTPDGNKsPAPKPSD Homo sapiens
pmid sentence
The actin binding properties of the minimal inhibitory region of caldesmon, residues 750-779, alter upon map kinase phosphorylation of ser-759. This phosphorylation leads to markedly diminished actin affinity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-71045 Ser789 QSVDKVTsPTKV Homo sapiens
pmid sentence
Extracellular signal-regulated kinases (erks) phosphorylate the high molecular mass isoform of the actin-binding protein caldesmon (h-cad) at two sites (ser(759) and ser(789)) during smooth muscle stimulation. Nmr spectroscopy shows that the actin binding properties of the minimal inhibitory region of caldesmon, residues 750-779, alter upon map kinase phosphorylation of ser-759, a residue not involved in actin binding. This phosphorylation leads to markedly diminished actin affinity as a result of the loss of interaction at one of the two sites that bind to f-actin.
Publications: 3 Organism: Homo Sapiens
Tissue: Smooth Muscle, Muscle, Smooth Muscle
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation FGFR1 0.323
Identifier Residue Sequence Organism Cell Line
SIGNOR-200884 Ser777 SMPLDQYsPSFPDTR Homo sapiens
pmid sentence
Erk-mediated phosphorylation of fibroblast growth factor receptor 1 on ser777 inhibits signaling
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation STAT5A 0.699
Identifier Residue Sequence Organism Cell Line
SIGNOR-66247 Ser780 DSLDSRLsPPAGLFT Homo sapiens
pmid sentence
Serine 780 is the only substrate in full-length stat5a for active erk
Publications: 1 Organism: Homo Sapiens
Tissue: Ovary
Pathways:Glucocorticoid receptor Signaling
+ MAPK3up-regulates img/direct-activation.png phosphorylation IL16 0.268
Identifier Residue Sequence Organism Cell Line
SIGNOR-121856 Ser845 SIRQRISsFETFGSS Homo sapiens
pmid sentence
The precursor form of the cytokine il-16 (proil-16) was shown to be phosphorylated on ser144 in antigen receptor-, sdf1alpha- and il-2-activated t cells. Genetic and pharmacological-inhibitor experiments showed that the phosphorylation of proil-16 is dependent on activation of the kinases erk1/2. Il-16 is secreted by mitogen-activated t cells, and the biochemical link between proil-16 and erk1/2, revealed by studies with pap-1, prompted analysis of the role of map kinases in this response.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation GABBR1 0.279
Identifier Residue Sequence Organism Cell Line
SIGNOR-277854 Ser868 ITRGEWQsEAQDTMK Homo sapiens HEK-293 Cell
pmid sentence
We found that, in addition to CaMKIIβ, also ERK1/2 is involved in the degradation pathway of GABAB receptors under physiological and ischemic conditions. In contrast to our previous view, CaMKIIβ does not appear to directly phosphorylate S867. Instead, the data support a mechanism in which CaMKIIβ activates ERK1/2, which then phosphorylates S867 and T872 in GABAB1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-277855 Thr873 WQSEAQDtMKTGSST Homo sapiens HEK-293 Cell
pmid sentence
We found that, in addition to CaMKIIβ, also ERK1/2 is involved in the degradation pathway of GABAB receptors under physiological and ischemic conditions. In contrast to our previous view, CaMKIIβ does not appear to directly phosphorylate S867. Instead, the data support a mechanism in which CaMKIIβ activates ERK1/2, which then phosphorylates S867 and T872 in GABAB1.
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation GSK3B 0.297
Identifier Residue Sequence Organism Cell Line
SIGNOR-262523 Ser9 SGRPRTTsFAESCKP Mus musculus MEF Cell
pmid sentence
We demonstrate that insulin-mediated activation of ERK1/2 results in phosphorylation of GSK3β at S9 independently of Akt/mTORC1 activity in Tsc2 null mouse embryonic fibroblasts. In addition, we show that inhibition of ERK1/2 rescues GSK3β activity and restores protein synthesis in Tsc2 −/− MEFs to normal levels
Publications: 1 Organism: Mus Musculus
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation PTPN7 0.685
Identifier Residue Sequence Organism Cell Line
SIGNOR-249475 Ser93 ALQRQPPsPKQLEEE in vitro
pmid sentence
First, Erk phosphorylates HePTP at residues Thr45 and Ser72. Second, HePTP dephosphorylates Erk at PTyr185.|
Identifier Residue Sequence Organism Cell Line
SIGNOR-249476 Thr66 EPICSVNtPREVTLH in vitro
pmid sentence
First, Erk phosphorylates HePTP at residues Thr45 and Ser72. Second, HePTP dephosphorylates Erk at PTyr185.|
Publications: 2 Organism: In Vitro
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation PLCB1 0.418
Identifier Residue Sequence Organism Cell Line
SIGNOR-106565 Ser982 KKKSEPSsPDHGSST in vitro
pmid sentence
Plc beta1 could be efficiently phosphorylated by activated mitogen-activated protein kinase but not by pka. The erk phosphorylation site was mapped to serine 982
Publications: 1 Organism: In Vitro
+ MAPK3up-regulates img/direct-activation.png phosphorylation MED1 0.265
Identifier Residue Sequence Organism Cell Line
SIGNOR-93989 Thr1032 SSSNRPFtPPTSTGG Homo sapiens
pmid sentence
Phosphorylation of transcriptional coactivator peroxisome proliferator-activated receptor (ppar)-binding protein (pbp). Stimulation of transcriptional regulation by mitogen-activated protein kinase
Identifier Residue Sequence Organism Cell Line
SIGNOR-93993 Thr1457 HSKSPAYtPQNLDSE Homo sapiens
pmid sentence
Phosphorylation of transcriptional coactivator peroxisome proliferator-activated receptor (ppar)-binding protein (pbp). Stimulation of transcriptional regulation by mitogen-activated protein kinase
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation SULT4A1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-168248 Thr11 SEAETPStPGEFESK Homo sapiens Neuron
pmid sentence
The phosphorylation of sult4a1 allows interaction with pin1, which then promotes degradation of the sulfotransferase.
Publications: 1 Organism: Homo Sapiens
Tissue: Brain
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation UBTF 0.568
Identifier Residue Sequence Organism Cell Line
SIGNOR-112813 Thr117 DFPKKPLtPYFRFFM Homo sapiens
pmid sentence
Erk1/2 was found to phosphorylate the architectural transcription factor ubf at amino acids 117 and 201 within hmg boxes 1 and 2, preventing their interaction with dna
Identifier Residue Sequence Organism Cell Line
SIGNOR-112817 Thr201 DIPEKPKtPQQLWYT Homo sapiens
pmid sentence
Erk1/2 was found to phosphorylate the architectural transcription factor ubf at amino acids 117 and 201 within hmg boxes 1 and 2, preventing their interaction with dna
Publications: 2 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation CASP9 0.545
Identifier Residue Sequence Organism Cell Line
SIGNOR-101548 Thr125 PEVLRPEtPRPVDIG Homo sapiens
pmid sentence
Inhibition of caspase-9 through phosphorylation at thr 125 by erk mapk
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MCL1 0.445
Identifier Residue Sequence Organism Cell Line
SIGNOR-179812 Thr163 TDGSLPStPPPAEEE Homo sapiens Breast Cancer Cell
pmid sentence
We then showed that erk could phosphorylate mcl-1 at two consensus residues, thr 92 and 163, which is required for the association of mcl-1 and pin1, resulting in stabilization of mcl-1.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation LAT 0.307
Identifier Residue Sequence Organism Cell Line
SIGNOR-125770 Thr184 PSAPALStPGIRDSA Homo sapiens
pmid sentence
Lat, an adapter protein essential for t-cell signaling, is phosphorylated at its thr 155 by erk in response to t-cell receptor stimulation. Thr 155 phosphorylation reduces the ability of lat to recruit plcgamma1 and slp76, leading to attenuation of subsequent downstream events such as [ca2+]i mobilization and activation of the erk pathway.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation CDKN1B 0.382
Identifier Residue Sequence Organism Cell Line
SIGNOR-80234 Thr187 NAGSVEQtPKKPGLR Homo sapiens Breast Cancer Cell
pmid sentence
These data suggest that increased signaling by erbb receptors up-regulates mapk activity, which, in turn, phosphorylates and destabilizes p27, thus contributing to dysregulated cell cycle progression.
Publications: 1 Organism: Homo Sapiens
+ MAP2K2up-regulates img/direct-activation.png phosphorylation MAPK3 0.735
Identifier Residue Sequence Organism Cell Line
SIGNOR-19240 Thr202 HDHTGFLtEYVATRW Homo sapiens
pmid sentence
The primary structure of mek, a protein kinase that phosphorylates the erk gene product
Identifier Residue Sequence Organism Cell Line
SIGNOR-19244 Tyr204 HTGFLTEyVATRWYR Homo sapiens
pmid sentence
The primary structure of mek, a protein kinase that phosphorylates the erk gene product
Publications: 2 Organism: Homo Sapiens
Tissue: Brain
+ PPP2CAdown-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.638
Identifier Residue Sequence Organism Cell Line
SIGNOR-144322 Thr202 HDHTGFLtEYVATRW Homo sapiens
pmid sentence
B56-containing pp2a dephosphorylate erk and their activity is controlled by the early gene iex-1 and erk
Identifier Residue Sequence Organism Cell Line
SIGNOR-103162 Homo sapiens
pmid sentence
P-erk1/2 proteins were efficiently dephosphorylated in vitro by protein phosphatases 1 and 2a (pp1/2a) and mapk phosphatase 3 (mkp3).
Publications: 2 Organism: Homo Sapiens
+ MEK1/2up-regulates img/direct-activation.png phosphorylation MAPK3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244802 Thr202 HDHTGFLtEYVATRW Homo sapiens
pmid sentence
The mek1 proline-rich insert is required for efficient activation of the mitogen-activated protein kinases erk1 and erk2 in mammalian cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244806 Tyr204 HTGFLTEyVATRWYR Homo sapiens
pmid sentence
The mek1 proline-rich insert is required for efficient activation of the mitogen-activated protein kinases erk1 and erk2 in mammalian cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244798 Homo sapiens
pmid sentence
Mek1 as indicated by extensive phosphorylation of erk1 and erk2 during the initial 2 h of adipogenesis.
Publications: 3 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation MAPK3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-249471 Thr202 HDHTGFLtEYVATRW
pmid sentence
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.|
Identifier Residue Sequence Organism Cell Line
SIGNOR-182628 Thr207 FLTEYVAtRWYRAPE Homo sapiens
pmid sentence
Here we show that autophosphorylation of erk1/2 on thr188 directs erk1/2 to phosphorylate nuclear targets known to cause cardiac hypertrophy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249472 Tyr204 HTGFLTEyVATRWYR
pmid sentence
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.|
Publications: 3 Organism: , Homo Sapiens
Tissue: Heart
Pathways:Glucocorticoid receptor Signaling
+ DUSP1down-regulates activity img/direct_inhibition.png dephosphorylation MAPK3 0.781
Identifier Residue Sequence Organism Cell Line
SIGNOR-248462 Thr202 HDHTGFLtEYVATRW Rattus norvegicus
pmid sentence
We demonstrate that ERK, JNK, and p38 are activated by distinct combinations of stimuli in T cells that simulate full or partial activation through the T cell receptor. These kinases are regulated by reversible phosphorylation on Tyr and Thr, and the dual specific phosphatases PAC1 and MKP-1 previously have been implicated in the in vivo inactivation of ERK or of ERK and JNK, respectively
Identifier Residue Sequence Organism Cell Line
SIGNOR-248463 Tyr204 HTGFLTEyVATRWYR Rattus norvegicus
pmid sentence
We demonstrate that ERK, JNK, and p38 are activated by distinct combinations of stimuli in T cells that simulate full or partial activation through the T cell receptor. These kinases are regulated by reversible phosphorylation on Tyr and Thr, and the dual specific phosphatases PAC1 and MKP-1 previously have been implicated in the in vivo inactivation of ERK or of ERK and JNK, respectively
Publications: 2 Organism: Rattus Norvegicus
+ DUSP4down-regulates activity img/direct_inhibition.png dephosphorylation MAPK3 0.701
Identifier Residue Sequence Organism Cell Line
SIGNOR-248715 Thr202 HDHTGFLtEYVATRW Rattus norvegicus
pmid sentence
Dephosphorylation and Inactivation of ERKs|ERK1 phosphorylated on either threonine (ERK1*Y204F) or tyrosine alone (ERK1*T202A) was utilized as a substrate for HVH2. Threonine 202 and tyrosine 204 in ERK1 (53) correspond to threonine 183 and tyrosine 185 in ERK2 which are the activation-phosphorylation sites by MEK(14, 15, 16). ERK1*, a kinase-deficient mutant, was phosphorylated on both threonine and tyrosine by MEK2 (Fig. 3B). ERK1*T202A, having threonine 202 substituted by an alanine, was phosphorylated only on tyrosine while ERK1*Y204F, having tyrosine 204 substituted by a phenylalanine, was phosphorylated only on threonine (Fig. 3B). GST-HVH2 dephosphorylated all three ERK1* mutants (Fig. 3A), suggesting that double phosphorylations of adjacent threonine and tyrosine were not a prerequisite for HVH2 recognition. However, HVH2 dephosphorylated ERK1* and ERK1*T202A more efficiently than ERK1*Y204F (Fig. 3A), indicating that HVH2 preferred phosphotyrosine over phosphothreonine. Interestingly, MEK also phosphorylated tyrosine residues more efficiently than threonine residues of ERK
Identifier Residue Sequence Organism Cell Line
SIGNOR-248716 Tyr204 HTGFLTEyVATRWYR Rattus norvegicus
pmid sentence
Dephosphorylation and Inactivation of ERKs|ERK1 phosphorylated on either threonine (ERK1*Y204F) or tyrosine alone (ERK1*T202A) was utilized as a substrate for HVH2. Threonine 202 and tyrosine 204 in ERK1 (53) correspond to threonine 183 and tyrosine 185 in ERK2 which are the activation-phosphorylation sites by MEK(14, 15, 16). ERK1*, a kinase-deficient mutant, was phosphorylated on both threonine and tyrosine by MEK2 (Fig. 3B). ERK1*T202A, having threonine 202 substituted by an alanine, was phosphorylated only on tyrosine while ERK1*Y204F, having tyrosine 204 substituted by a phenylalanine, was phosphorylated only on threonine (Fig. 3B). GST-HVH2 dephosphorylated all three ERK1* mutants (Fig. 3A), suggesting that double phosphorylations of adjacent threonine and tyrosine were not a prerequisite for HVH2 recognition. However, HVH2 dephosphorylated ERK1* and ERK1*T202A more efficiently than ERK1*Y204F (Fig. 3A), indicating that HVH2 preferred phosphotyrosine over phosphothreonine. Interestingly, MEK also phosphorylated tyrosine residues more efficiently than threonine residues of ERK
Publications: 2 Organism: Rattus Norvegicus
+ MAP2K1up-regulates img/direct-activation.png phosphorylation MAPK3 0.745
Identifier Residue Sequence Organism Cell Line
SIGNOR-59153 Thr202 HDHTGFLtEYVATRW Homo sapiens
pmid sentence
The mek1 proline-rich insert is required for efficient activation of the mitogen-activated protein kinases erk1 and erk2 in mammalian cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-59157 Tyr204 HTGFLTEyVATRWYR Homo sapiens
pmid sentence
The mek1 proline-rich insert is required for efficient activation of the mitogen-activated protein kinases erk1 and erk2 in mammalian cells.
Identifier Residue Sequence Organism Cell Line
SIGNOR-210176 Homo sapiens
pmid sentence
Mek1 as indicated by extensive phosphorylation of erk1 and erk2 during the initial 2 h of adipogenesis.
Publications: 3 Organism: Homo Sapiens
+ ERK1/2up-regulates activity img/direct-activation.png phosphorylation MAPK3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244565 Thr207 FLTEYVAtRWYRAPE Homo sapiens
pmid sentence
Here we show that autophosphorylation of erk1/2 on thr188 directs erk1/2 to phosphorylate nuclear targets known to cause cardiac hypertrophy.
Publications: 1 Organism: Homo Sapiens
Tissue: Heart
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation MBP 0.511
Identifier Residue Sequence Organism Cell Line
SIGNOR-22424 Thr232 KNIVTPRtPPPSQGK Homo sapiens
pmid sentence
Phosphorylation decreased the ability of mbp to polymerize actin and to bundle actin filaments but had no effect on the dissociation constant of the mbp-actin complex or on the ability of ca2+-calmodulin to dissociate the complex. The most significant effect of phosphorylation on the mbp-actin complex was a dramatic reduction in its ability to bind to negatively charged lipid bilayers. The identification of myelin basic protein (phosphorylation at -pro-arg-thr-pro-) as a substrate for the erk kinases (fig. 1) demonstrates that there are other determinants important for substrate recognition than those present in the originally identified consensus sequence.
Identifier Residue Sequence Organism Cell Line
SIGNOR-143481 Thr232 KNIVTPRtPPPSQGK Homo sapiens
pmid sentence
Phosphorylation decreased the ability of mbp to polymerize actin and to bundle actin filaments but had no effect on the dissociation constant of the mbp-actin complex or on the ability of ca2+-calmodulin to dissociate the complex. The most significant effect of phosphorylation on the mbp-actin complex was a dramatic reduction in its ability to bind to negatively charged lipid bilayers. The identification of myelin basic protein (phosphorylation at -pro-arg-thr-pro-) as a substrate for the erk kinases (fig. 1) demonstrates that there are other determinants important for substrate recognition than those present in the originally identified consensus sequence.
Publications: 2 Organism: Homo Sapiens
Tissue: Brain
+ MAPK3up-regulates img/direct-activation.png phosphorylation FOS 0.711
Identifier Residue Sequence Organism Cell Line
SIGNOR-33909 Thr232 GGLPEVAtPESEEAF Homo sapiens
pmid sentence
Phosphorylation of the c-fos and c-jun hob1 motif stimulates its activation capacity here we show that the hob1-containing activation domain of c-fos is stimulated by ha-ras in vivo and phosphorylated by a map kinase family member in vitro and that mutating t232 to ala abolishes both functions.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation CEBPB 0.66
Identifier Residue Sequence Organism Cell Line
SIGNOR-184917 Thr235 SSSSPPGtPSPADAK Homo sapiens
pmid sentence
Thr235 phosphorylation occurs in nuclei of differentiated macrophages, but not in monocytes.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation TRPV3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273672 Thr264 EGFYFGEtPLALAAC Homo sapiens HaCaT Cell
pmid sentence
We observed that ERK-mediated phosphorylation of TRPV3 alters its responsiveness to repeated chemical stimuli. Among several putative ERK phosphorylation sites, we identified threonine 264 in the N-terminal ankyrin repeat domain as the most critical site for the ERK-dependent modulation of TRPV3 channel activity. Of note, Thr264 is in close vicinity to a structurally and functionally important TRPV3 region comprising an atypical finger 3 and oxygen-dependent hydroxylation site. In summary, our findings indicate that Thr264 in TRPV3 is a key ERK phosphorylation site mediating EGFR-induced sensitization of the channel to stimulate signaling pathways involved in regulating skin homeostasis.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation SMAD4 0.426
Identifier Residue Sequence Organism Cell Line
SIGNOR-101664 Thr277 GSRTAPYtPNLPHHQ Homo sapiens
pmid sentence
Our results suggest that map kinase can phosphorylate thr276 of smad4 and that phosphorylation can lead to enhanced tgf-beta-induced nuclear accumulation and, as a consequence, enhanced transcriptional activity of smad4.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation PAK1 0.341
Identifier Residue Sequence Organism Cell Line
SIGNOR-123074 Thr292 YTAMDVAtGQEVAIK Homo sapiens
pmid sentence
Activated erk can phosphorylate t292 in the prs, and this blocks the ability of pak to phosphorylate s298 and of rac-pak signaling to enhance mek1-erk complex formation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation TCF3 0.377
Identifier Residue Sequence Organism Cell Line
SIGNOR-249117 Thr355 NFSSSPStPVGSPQG Mus musculus
pmid sentence
Notch-induced degradation requires phosphorylation of E47 by p42/p44 MAP kinases. |Wild_type E47 but not the Mm mutant reacted to the antibodies, suggesting that E47 is at least phosphorylated at the M2 site (Figure 3A)|anti_phospho_M2 peptide (SSPSpTPVGSPQG)
Publications: 1 Organism: Mus Musculus
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation PTPRR 0.661
Identifier Residue Sequence Organism Cell Line
SIGNOR-249477 Thr361 EPFVSIPtPREKVAM
pmid sentence
Specifically, the complex formation between PTP-SL and ERK2 involves an unusual interaction leading to the phosphorylation of PTP-SL by ERK2 at Thr253 and the inactivating dephosphorylation of ERK2 by PTP-SL.
Publications: 1
+ MAPK3up-regulates img/direct-activation.png phosphorylation ETS1 0.664
Identifier Residue Sequence Organism Cell Line
SIGNOR-116494 Thr38 CADVPLLtPSSKEMM Homo sapiens
pmid sentence
We found that hgf/sf activates the erk1 map kinase, leading to the phosphorylation of the threonine 38 residue of ets1
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MKNK1 0.564
Identifier Residue Sequence Organism Cell Line
SIGNOR-48360 Thr385 APEKGLPtPQVLQRN Homo sapiens HeLa Cell
pmid sentence
Mnk1 was phosphorylated and activated in vitro by erk1 and p38 map kinasespreliminary results showed that thr344 at least was one of the major sites phosphorylated by erk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-48352 Homo sapiens
pmid sentence
We have identified a new subfamily of murine serine/threonine kinases, whose members, map kinase-interacting kinase 1 (mnk1) and mnk2, bind tightly to the growth factor-regulated map kinases, erk1 and erk2.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation RPS3 0.356
Identifier Residue Sequence Organism Cell Line
SIGNOR-137175 Thr42 SGVEVRVtPTRTEII Homo sapiens
pmid sentence
Erk phosphorylates threonine 42 residue of ribosomal protein s3.
Publications: 1 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation RPS3 0.356
Identifier Residue Sequence Organism Cell Line
SIGNOR-137959 Thr42 SGVEVRVtPTRTEII Homo sapiens
pmid sentence
Erk phosphorylates threonine 42 residue of ribosomal protein s3.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation GSK3B 0.297
Identifier Residue Sequence Organism Cell Line
SIGNOR-138898 Thr43 KVTTVVAtPGQGPDR Homo sapiens Breast Cancer Cell, Kidney Cancer Cell
pmid sentence
Erk, which is activated by hbx, associates with gsk-3beta through a docking motif ((291)fkfp) of gsk-3beta and phosphorylates gsk-3beta at the (43)thr residue, which primes gsk-3beta for its subsequent phosphorylation at ser9 by p90rsk, resulting in inactivation of gsk-3beta and upregulation of beta-catenin.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation SP1 0.644
Identifier Residue Sequence Organism Cell Line
SIGNOR-249480 Thr453 SGPIIIRtPTVGPNG Homo sapiens
pmid sentence
Transcriptional activation of p21(waf1/cip1) by alkylphospholipids: role of the mitogen-activated protein kinase pathway in the transactivation of the human p21(waf1/cip1) promoter by Sp1.|this activation promotes the phosphorylation of Sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased Sp1 binding and enhanced p21(waf1/cip1) transcription.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249481 Thr739 SEGSGTAtPSALITT Homo sapiens
pmid sentence
Transcriptional activation of p21(waf1/cip1) by alkylphospholipids: role of the mitogen-activated protein kinase pathway in the transactivation of the human p21(waf1/cip1) promoter by Sp1.|this activation promotes the phosphorylation of Sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased Sp1 binding and enhanced p21(waf1/cip1) transcription.
Publications: 2 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation CAD 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-137179 Thr456 KVYFLPItPHYVTQV Homo sapiens
pmid sentence
Cad is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. The activation of the pathway required for cell proliferation is a consequence of the phosphorylation of cad thr-456 by mitogen-activated protein (map) kinase.Activated map kinase (erk1/2), the enzyme responsible for the phosphorylation of thr-456, was also present in larger amounts in the nucleus than the cytosol
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation SHOC2 0.339
Identifier Residue Sequence Organism Cell Line
SIGNOR-277443 Thr507 GLGENLLtHLPEEIG Homo sapiens HEK-293 Cell
pmid sentence
Here, we showed that SHOC2, a RAS activator, is a FBXW7 substrate. Growth stimuli trigger SHOC2 phosphorylation on Thr507 by the mitogen-activated protein kinase (MAPK) signal, which facilitates FBXW7 binding for ubiquitylation and degradation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates quantity by stabilization img/direct-activation.png phosphorylation BCL2 0.548
Identifier Residue Sequence Organism Cell Line
SIGNOR-74939 Thr56 FSSQPGHtPHPAASR Homo sapiens
pmid sentence
Phosphorylation of the map kinase sites in bcl-2, thr56, thr74, and ser87, is sufficient to inhibit tnf--induced degradation. p44mapk/extracellular signal-regulated kinase 1 (erk1) and p42 mapk/erk2 are activated by il-3, colocalize with mitochondrial bcl2, and can directly phosphorylate bcl2 on ser-70 in a stauro-resistant manner both_ in vitro_ and_ in vivo.
Identifier Residue Sequence Organism Cell Line
SIGNOR-74943 Thr74 ARTSPLQtPAAPGAA Homo sapiens
pmid sentence
The results of this study reveal the following novel findings: destruction of the three putative MAP kinase sites at positions 56, 74, and 87 results in ubiquitination and subsequent degradation of the protein. Progressive inactivation of these MAP kinase sites revealed that Bcl-2 stability is mainly regulated by phosphorylation at Thr74 and Ser87, with Ser87 phosphorylation playing a predominant role. TNF-α or the MAP kinase-specific inhibitor PD98059 diminishes Ser87 phosphorylation of Bcl-2 in vivo, while activated ERK2 induces phosphorylation of Bcl-2 in vivo and in vitro.
Publications: 2 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation SPIB 0.314
Identifier Residue Sequence Organism Cell Line
SIGNOR-41800 Thr56 VAPPVPAtPYEAFDP Homo sapiens B-lymphocyte
pmid sentence
The threonine 56 was defined as the erk1 phosphorylation site by using phosphoamino-acid analyses and a spi-b mutant version
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation SCNN1B 0.28
Identifier Residue Sequence Organism Cell Line
SIGNOR-249447 Thr615 QALPIPGtPPPNYDS in vitro
pmid sentence
Using a number of different approaches it was demonstrated that the protein kinase acting on betaThr-613 and gammaThr-623 is the extracellular regulated kinase (ERK). It is suggested that an ERK-mediated phosphorylation of betaThr-613 and gammaThr-623 down-regulates the channel by facilitating its interaction with Nedd4.
Publications: 1 Organism: In Vitro
+ MAPK3down-regulates quantity by destabilization img/direct_inhibition.png phosphorylation SCNN1G 0.282
Identifier Residue Sequence Organism Cell Line
SIGNOR-249449 Thr622 LGTQVPGtPPPKYNT in vitro
pmid sentence
Using a number of different approaches it was demonstrated that the protein kinase acting on betaThr-613 and gammaThr-623 is the extracellular regulated kinase (ERK). It is suggested that an ERK-mediated phosphorylation of betaThr-613 and gammaThr-623 down-regulates the channel by facilitating its interaction with Nedd4.
Publications: 1 Organism: In Vitro
+ MAPK3up-regulates img/direct-activation.png phosphorylation ARHGEF2 0.292
Identifier Residue Sequence Organism Cell Line
SIGNOR-160420 Thr679 PGVELLLtPREPALP Homo sapiens HeLa Cell
pmid sentence
Activates rhoa and as a result regulates actin assembly.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation ATF2 0.732
Identifier Residue Sequence Organism Cell Line
SIGNOR-163254 Thr69 SVIVADQtPTPTRFL Homo sapiens
pmid sentence
Phosphorylation of thr-69 by mapk14 and mapk11, and at thr-71 by mapk1/erk2, mapk3/erk1, mapk11, mapk12 and mapk14 in response to external stimulus like insulin causes increased transcriptional activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-90529 Thr69 SVIVADQtPTPTRFL Homo sapiens
pmid sentence
Here, we show that in fibroblasts, insulin, epidermal growth factor (egf) and serum activate atf2 via a so far unknown two-step mechanism involving two distinct ras effector pathways: the raf-mek-erk pathway induces phosphorylation of atf2 thr71, whereas subsequent atf2 thr69 phosphorylation requires the ral-ralgds-src-p38 pathway.
Identifier Residue Sequence Organism Cell Line
SIGNOR-163258 Thr71 IVADQTPtPTRFLKN Homo sapiens
pmid sentence
Phosphorylation of thr-69 by mapk14 and mapk11, and at thr-71 by mapk1/erk2, mapk3/erk1, mapk11, mapk12 and mapk14 in response to external stimulus like insulin causes increased transcriptional activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-90533 Thr71 IVADQTPtPTRFLKN Homo sapiens
pmid sentence
Here, we show that in fibroblasts, insulin, epidermal growth factor (egf) and serum activate atf2 via a so far unknown two-step mechanism involving two distinct ras effector pathways: the raf-mek-erk pathway induces phosphorylation of atf2 thr71, whereas subsequent atf2 thr69 phosphorylation requires the ral-ralgds-src-p38 pathway.
Publications: 4 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation EGFR 0.546
Identifier Residue Sequence Organism Cell Line
SIGNOR-20549 Thr693 RELVEPLtPSGEAPN Homo sapiens
pmid sentence
It is likely that the map2 and ert kinases account for the phosphorylation of the egf receptor at thr669 (egf receptor (krel veplt669psgeapnqallr)) observed in cultured cells.Phosphorylation at ser-695 is partial and occurs only if thr-693 is phosphorylated. Phosphorylation at thr-678 and thr-693 by prkd1 inhibits egf-induced mapk8/jnk1 activation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MAZ 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-114475 Thr72 AAPAPPPtPQAPAAE Homo sapiens
pmid sentence
Together, these results show that activation of saf-1 in response to il-1 and -6 is mediated via map kinase-regulated phosphorylation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation ADAM17 0.367
Identifier Residue Sequence Organism Cell Line
SIGNOR-89625 Thr735 KPFPAPQtPGRLQPA Homo sapiens
pmid sentence
Extracellular signal-regulated kinase phosphorylates tumor necrosis factor alpha-converting enzyme at threonine 735: a potential role in regulated sheddingwe show that extracellular signal-regulated kinase (erk) acts as an intermediate in protein kinase c-regulated trka cleavage. We report that the cytosolic tail of the tumor necrosis factor alpha-converting enzyme (tace) is phosphorylated by erk at threonine 735. In addition, we show that erk and tace associate. This association is favored by erk activation and by the presence of threonine 735. In contrast to the erk route, the p38 mapk was able to stimulate trka cleavage in cells devoid of tace activity, indicating that other proteases are also involved in trka shedding.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation BRAF 0.635
Identifier Residue Sequence Organism Cell Line
SIGNOR-144827 Thr753 YACASPKtPIQAGGY Homo sapiens
pmid sentence
Erk-induced phosphorylation of b-raf on t753 promoted the disassembly of raf heterodimers, and the mutation of t753 prolonged growth factor-induced heterodimerization. The b-raf t753a mutant enhanced differentiation of pc12 cells, which was previously shown to be dependent on sustained erk signaling. Site is critical for v-src dependent modulation of slk kinase activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-161819 Thr753 YACASPKtPIQAGGY Homo sapiens
pmid sentence
Erk-induced phosphorylation of b-raf on t753 promoted the disassembly of raf heterodimers, and the mutation of t753 prolonged growth factor-induced heterodimerization. The b-raf t753a mutant enhanced differentiation of pc12 cells, which was previously shown to be dependent on sustained erk signaling. Site is critical for v-src dependent modulation of slk kinase activity.
Publications: 2 Organism: Homo Sapiens
+ MAPK3 img/unknown.png phosphorylation EWSR1 0.267
Identifier Residue Sequence Organism Cell Line
SIGNOR-182782 Thr79 QPPTGYTtPTAPQAY Homo sapiens
pmid sentence
Here we report that ews and ews-fli1 become phosphorylated at thr79 in the n-terminal domain in response to mitogens or dna damage. Mitogen-induced phosphorylation of ews and ews-fli1 was weak and catalysed by erk1 (extracellular signal-regulated kinase 1) and erk2.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation SMAD2 0.742
Identifier Residue Sequence Organism Cell Line
SIGNOR-227514 Thr8 MSSILPFtPPVVKRL Homo sapiens
pmid sentence
We show that phosphorylation of Smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (ERK1) increases the amount of Smad2 protein and leads to enhanced transcriptional activity.[] A site of ERK-dependent phosphorylation on Smad2 was located to Thr8
Identifier Residue Sequence Organism Cell Line
SIGNOR-217613 Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-236067 Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3 .we show that phosphorylation of smad2, a mediator of the activin/transforming growth factor-beta signal, by activated extracellular signal-regulated kinase 1 (erk1) increases the amount of smad2 protein and leads to enhanced transcriptional activity
Publications: 3 Organism: Homo Sapiens
Tissue: Lung, Breast
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation LIPE 0.421
Identifier Residue Sequence Organism Cell Line
SIGNOR-249470 Thr891 NSETSSDtPEMSLSA Mus musculus NIH-3T3 Cell
pmid sentence
Thus, activation of the ERK pathway appears to be able to regulate adipocyte lipolysis by phosphorylating HSL on Ser(600) and increasing the activity of HSL.
Publications: 1 Organism: Mus Musculus
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation CTNND1 0.297
Identifier Residue Sequence Organism Cell Line
SIGNOR-277506 Thr906 SLDNNYStPNERGDH Canis lupus familiaris MDCK Cell
pmid sentence
 Upon TGFβ treatment, activated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylates T900 of p120-catenin to promote its interaction with Smurf1 and subsequent monoubiquitination. TGFβ promotes monoubiquitination of p120-catenin through Smurf1 to induce junction dissociation.
Publications: 1 Organism: Canis Lupus Familiaris
+ DUSP3down-regulates activity img/direct_inhibition.png dephosphorylation MAPK3 0.656
Identifier Residue Sequence Organism Cell Line
SIGNOR-103035 Tyr204 HTGFLTEyVATRWYR Homo sapiens Dermal Fibroblast
pmid sentence
The activation of the mapk activity requires the dual phosphorylation of the ser/thr and tyr residues in the txy kinase activation motif (1113), and deactivation occurs through the action of either ser/thr protein phosphatase (14), protein-tyrosine phosphatase (ptp) (14, 15), or dual specificity phosphatases
Identifier Residue Sequence Organism Cell Line
SIGNOR-248535 Tyr204 HTGFLTEyVATRWYR Chlorocebus aethiops COS Cell
pmid sentence
Extracellular regulated kinases (ERK) 1 and ERK2 are authentic substrates for the dual-specificity protein-tyrosine phosphatase VHR. A novel role in down-regulating the ERK pathway.|Catalysis by VHR requires the native structure of ERK and is specific for tyrosine 185 of ERK2
Publications: 2 Organism: Homo Sapiens, Chlorocebus Aethiops
+ LCKup-regulates img/direct-activation.png phosphorylation MAPK3 0.554
Identifier Residue Sequence Organism Cell Line
SIGNOR-159168 Tyr204 HTGFLTEyVATRWYR Homo sapiens
pmid sentence
The sh3 domain of lck modulates t-cell receptor-dependent activation of extracellular signal-regulated kinase through activation of raf-1.
Publications: 1 Organism: Homo Sapiens
Pathways:Glucocorticoid receptor Signaling
+ RETup-regulates img/direct-activation.png phosphorylation MAPK3 0.439
Identifier Residue Sequence Organism Cell Line
SIGNOR-140298 Tyr204 HTGFLTEyVATRWYR Homo sapiens
pmid sentence
We hypothesized that ret could directly phosphorylate fak and erk. erk 2 could be phosphorylated at y187 (y204 in erk1).
Publications: 1 Organism: Homo Sapiens
+ PTPRJdown-regulates activity img/direct_inhibition.png dephosphorylation MAPK3 0.466
Identifier Residue Sequence Organism Cell Line
SIGNOR-248707 Tyr204 HTGFLTEyVATRWYR in vitro
pmid sentence
Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhibits the RAS pathway by direct dephosphorylation of ERK1/2 kinases|Pulldown and in vitro dephosphorylation assays confirmed our prediction and demonstrated an overall specificity of DEP-1 in targeting the phosphorylated tyrosine 204 of ERK1/2.
Publications: 1 Organism: In Vitro
+ PP1down-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-264665 Homo sapiens
pmid sentence
P-erk1/2 proteins were efficiently dephosphorylated in vitro by protein phosphatases 1 and 2a (pp1/2a) and mapk phosphatase 3 (mkp3). the dual specificity phosphatases that specifically dephosphorylate and inactivate the p-erk1/2 are called mapk phosphatases
Publications: 1 Organism: Homo Sapiens
+ PTPRJdown-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.466
Identifier Residue Sequence Organism Cell Line
SIGNOR-101282 Homo sapiens
pmid sentence
A dominant-negative mutant of high cell densityenhanced ptp 1 (dep-1)//cd148 as well as reduction of its expression by rna interference partially restore vegfr-2 phosphorylation and map kinase activation.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation RPS6KA4 0.574
Identifier Residue Sequence Organism Cell Line
SIGNOR-60998 Homo sapiens
pmid sentence
Rsk-b is a p38alphamapk substrate, and activated by p38alphamapk and, more weakly, by erk1
Publications: 1 Organism: Homo Sapiens
+ CDC25Adown-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.401
Identifier Residue Sequence Organism Cell Line
SIGNOR-133392 Homo sapiens MCF-7 Cell
pmid sentence
We found that cdc25a physically interacted with and de-phosphorylated phospho-erk both in vitro and in cell culture.
Publications: 1 Organism: Homo Sapiens
+ LAMTOR3up-regulates img/direct-activation.png binding MAPK3 0.596
Identifier Residue Sequence Organism Cell Line
SIGNOR-59877 Homo sapiens
pmid sentence
A protein called mp1 (mek partner 1) was identified that bound specifically to mek1 and erk1 and facilitated their activation. When overexpressed in cultured cells, mp1 enhanced activation of erk1 and activation of a reporter driven by the transcription factor elk-1.
Publications: 1 Organism: Homo Sapiens
+ NR3C1down-regulates activity img/indirect_inhibition.png MAPK3 0.53
Identifier Residue Sequence Organism Cell Line
SIGNOR-251677 Mus musculus NIH-3T3 Cell
pmid sentence
Both induction of MKP-1 expression and inhibition of its degradation are necessary for glucocorticoid-mediated inhibition of Erk-1/2 activation.
Publications: 1 Organism: Mus Musculus
Pathways:Glucocorticoid receptor Signaling
+ MAPK3up-regulates img/direct-activation.png phosphorylation mTORC1 0.39
Identifier Residue Sequence Organism Cell Line
SIGNOR-217559 Homo sapiens
pmid sentence
Here we focus primarily although not exclusively on raptor Ser(863) phosphorylation. We report that insulin promotes mTORC1-associated phosphorylation of raptor Ser(863) via the canonical PI3K/TSC/Rheb pathway in a rapamycin-sensitive manner. mTORC1 activation by other stimuli (e.g. amino acids, epidermal growth factor/MAPK signaling, and cellular energy) also promote raptor Ser(863) phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-217556 Homo sapiens
pmid sentence
The phosphorylation of raptor is stimulated by insulin and inhibited by rapamycin. Importantly, the site-directed mutation of raptor at one phosphorylation site, Ser(863), reduced mTORC1 activity both in vitro and in vivo.
Identifier Residue Sequence Organism Cell Line
SIGNOR-217544 Homo sapiens
pmid sentence
The phosphorylation of Raptor on these sites enhances mTORC1 activity, and contributes largely to arsenite-induced mTORC1 activation.
Publications: 3 Organism: Homo Sapiens
+ PEA15down-regulates img/indirect_inhibition.png MAPK3 0.762
Identifier Residue Sequence Organism Cell Line
SIGNOR-111502 Homo sapiens
pmid sentence
Here, we report that pea-15, a protein variably expressed in multiple cell types, blocks erk-dependent transcription and proliferation by binding erks and preventing their localization in the nucleus._ Pea-15 can redirect the biological outcome of map kinase signaling by regulating the subcellular localization of erk map kinase.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation SMAD3 0.614
Identifier Residue Sequence Organism Cell Line
SIGNOR-66781 Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3.
Publications: 1 Organism: Homo Sapiens
Tissue: Lung, Breast
+ MAPK3up-regulates img/direct-activation.png phosphorylation LRP6 0.317
Identifier Residue Sequence Organism Cell Line
SIGNOR-169004 Homo sapiens
pmid sentence
We show that several proline-directed mitogen-activated protein kinases (mapks), such as p38, erk1/2, and jnk1 are sufficient and required for the phosphorylation of ppps/tp motifs of lrp6.
Publications: 1 Organism: Homo Sapiens
+ SHC1up-regulates activity img/indirect-activation.png MAPK3 0.62
Identifier Residue Sequence Organism Cell Line
SIGNOR-242625 Mus musculus NIH-3T3 Cell
pmid sentence
We report that upon TGF__ stimulation, the activated TGF__ type I receptor (T_RI) recruits and directly phosphorylates ShcA proteins on tyrosine and serine. This dual phosphorylation results from an intrinsic T_RI tyrosine kinase activity that complements its well_defined serine_threonine kinase function. TGF___induced ShcA phosphorylation induces ShcA association with Grb2 and Sos, thereby initiating the well_characterised pathway linking receptor tyrosine kinases with Erk MAP kinases.
Publications: 1 Organism: Mus Musculus
+ MAPK3 img/unknown.png phosphorylation mTORC1 0.39
Identifier Residue Sequence Organism Cell Line
SIGNOR-217577 Homo sapiens
pmid sentence
We found three proline-directed residues within raptor, ser(8), ser(696), and ser(863), which are directly phosphorylated by erk1/2. Expression of phosphorylation-deficient alleles of raptor revealed that phosphorylation of these sites by erk1/2 normally promotes mtorc1 activity and signaling to downstream substrates, such as 4e-bp1.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation THR 0.432
Identifier Residue Sequence Organism Cell Line
SIGNOR-270301 Homo sapiens
pmid sentence
We concluded that serine 142 of the tr dbd is the likely site of phosphorylation by t(4)-activated mapk and that the docking site on tr for activated mapk includes residues 128-133 (kgffrr), a basic amino acid-enriched motif novel for mapk substrates. Tr mutations in the proposed mapk docking domain and at residue 142 modulated t(4)-conditioned shedding of co-repressor and recruitment of co-activator proteins by the receptor, and they altered transcriptional activity of tr in a thyroid hormone response element-luciferase reporter assay.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation BAD 0.469
Identifier Residue Sequence Organism Cell Line
SIGNOR-188172 Homo sapiens HeLa Cell
pmid sentence
Erk-1 map kinase prevents tnf-induced apoptosis through bad phosphorylation and inhibition of bax translocation in hela cells.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates activity img/direct_inhibition.png phosphorylation GSK3B/Axin/APC 0.353
Identifier Residue Sequence Organism Cell Line
SIGNOR-262520 Mus musculus MEF Cell
pmid sentence
We demonstrate that insulin-mediated activation of ERK1/2 results in phosphorylation of GSK3β at S9 independently of Akt/mTORC1 activity in Tsc2 null mouse embryonic fibroblasts. In addition, we show that inhibition of ERK1/2 rescues GSK3β activity and restores protein synthesis in Tsc2 −/− MEFs to normal levels
Publications: 1 Organism: Mus Musculus
+ MAPK3up-regulates img/direct-activation.png phosphorylation E2F1 0.295
Identifier Residue Sequence Organism Cell Line
SIGNOR-198292 Homo sapiens Breast Cancer Cell
pmid sentence
Erk also undergoes rapid translocation into the nucleus, where it phosphorylates and activates a variety of transcription factor targets, including sp1, e2f, elk-1, and ap1
Identifier Residue Sequence Organism Cell Line
SIGNOR-121991 Homo sapiens
pmid sentence
Erk also undergoes rapid translocation into the nucleus, where it phosphorylates and activates a variety of transcription factor targets, including sp1, e2f, elk-1, and ap1
Publications: 2 Organism: Homo Sapiens
+ DUSP23down-regulates activity img/direct_inhibition.png dephosphorylation MAPK3 0.313
Identifier Residue Sequence Organism Cell Line
SIGNOR-277103 Homo sapiens
pmid sentence
In particular, DUSP23 can dephosphorylate and inactivate MAPK3 ( xref ).|In particular, DUSP23 can dephosphorylate and inactivate MAPK3.
Publications: 1 Organism: Homo Sapiens
+ WDR83up-regulates img/direct-activation.png binding MAPK3 0.494
Identifier Residue Sequence Organism Cell Line
SIGNOR-124473 Homo sapiens
pmid sentence
Morg1 specifically associates with several components of the erk pathway, including mp1, raf-1, mek, and erk, and stabilizes their assembly into an oligomeric complex.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation SMAD2 0.742
Identifier Residue Sequence Organism Cell Line
SIGNOR-66778 Homo sapiens
pmid sentence
These results suggest that oncogenic ras, acting through mek1 and erk kinases, induces the phosphorylation of smad2 and smad3
Publications: 1 Organism: Homo Sapiens
+ 17beta-estradiolup-regulates img/indirect-activation.png MAPK3 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-83280 Homo sapiens Breast Cancer Cell
pmid sentence
Estrogen rapidly activates the mitogen-activated protein kinases, erk-1 and erk-2, via an as yet unknown mechanism.
Publications: 1 Organism: Homo Sapiens
+ MAPK3down-regulates img/direct_inhibition.png phosphorylation SMAD1 0.516
Identifier Residue Sequence Organism Cell Line
SIGNOR-66755 Homo sapiens
pmid sentence
Ras signaling was shown previously to induce the phosphorylation of the bmp mediator smad1 at four erk consensus sites in the linker domain (kretzschmar et al. 1997a). Phosphorylation of these four sites inhibits smad1 accumulation in the nucleus
Publications: 1 Organism: Homo Sapiens
+ DUSP1down-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.781
Identifier Residue Sequence Organism Cell Line
SIGNOR-73617 Homo sapiens
pmid sentence
The mitogen-activated protein (map) kinase cascade is inactivated at the level of map kinase by members of the map kinase phosphatase (mkp) family, including mkp-1.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation RPS6KA2 0.711
Identifier Residue Sequence Organism Cell Line
SIGNOR-44949 Homo sapiens
pmid sentence
Several lines of investigation have suggested that rsk is phosphorylated and activated by erk1/2 mapk isoforms
Publications: 1 Organism: Homo Sapiens
+ MEK1/2up-regulates activity img/direct-activation.png phosphorylation MAPK3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-258991 Mus musculus
pmid sentence
Raf proteins have been shown to phosphorylate and activate MAPKKs (MAP kinase kinases) called MEKs (MAPK or ERK kinases) which in turn phosphorylate and activate MAPKs (MAP kinases) called ERKs
Publications: 1 Organism: Mus Musculus
+ MAPK3down-regulates activity img/direct_inhibition.png BRD4 0.315
Identifier Residue Sequence Organism Cell Line
SIGNOR-262048 Homo sapiens
pmid sentence
The MYC stabilizing kinase, ERK1, regulates MYC levels directly and indirectly by inhibiting BRD4 kinase activity.
Publications: 1 Organism: Homo Sapiens
+ FN1up-regulates img/indirect-activation.png MAPK3 0.595
Identifier Residue Sequence Organism Cell Line
SIGNOR-165350 Homo sapiens
pmid sentence
We conclude that, by interacting with fibronectin, pref-1 activates integrin downstream signaling to activate mek/erk and to inhibit adipocyte differentiation.
Publications: 1 Organism: Homo Sapiens
+ DUSP9down-regulates img/direct_inhibition.png binding MAPK3 0.696
Identifier Residue Sequence Organism Cell Line
SIGNOR-176589 Homo sapiens
pmid sentence
Here we demonstrate that inactivation of both erk1/2 and p38_ by dusp9/mkp-4 is mediated by a conserved arginine-rich kinase interaction motif located within the amino-terminal non-catalytic domain of the protein.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation NCKIPSD 0.428
Identifier Residue Sequence Organism Cell Line
SIGNOR-118747 Homo sapiens
pmid sentence
Spin90 was phosphorylated by erk1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of spin90 likely promotes the interaction of the spin90.betapix.wasp complex and nck
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MAPKAPK2 0.482
Identifier Residue Sequence Organism Cell Line
SIGNOR-201687 Homo sapiens
pmid sentence
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase
Identifier Residue Sequence Organism Cell Line
SIGNOR-121994 Homo sapiens
pmid sentence
Erk phosphorylates multiple cytoplasmatic and cytoskeletal proteins, including mapk-activated protein kinases and the ribosomal p70-s6 kinase
Publications: 2 Organism: Homo Sapiens
+ FGF2up-regulates img/indirect-activation.png MAPK3 0.593
Identifier Residue Sequence Organism Cell Line
SIGNOR-168995 Homo sapiens
pmid sentence
We show that several proline-directed mitogen-activated protein kinases (mapks), such as p38, erk1/2, and jnk1 are sufficient and required for the phosphorylation of ppps/tp motifs of lrp6. External stimuli, which control the activity of mapks, such as phorbol esters and fibroblast growth factor 2 (fgf2) control the choice of the lrp6-ppps/tp kinase and regulate the amplitude of lrp6 phosphorylation and wnt/beta-catenin-dependent transcription.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation mTORC1 0.39
Identifier Residue Sequence Organism Cell Line
SIGNOR-209859 Homo sapiens
pmid sentence
Activation of mTORC1 in two steps: Rheb-GTP activation of catalytic function and increased binding of substrates to raptor.
Publications: 1 Organism: Homo Sapiens
+ MAP2K2up-regulates activity img/direct-activation.png phosphorylation MAPK3 0.735
Identifier Residue Sequence Organism Cell Line
SIGNOR-258997 Mus musculus
pmid sentence
Raf proteins have been shown to phosphorylate and activate MAPKKs (MAP kinase kinases) called MEKs (MAPK or ERK kinases) which in turn phosphorylate and activate MAPKs (MAP kinases) called ERKs
Publications: 1 Organism: Mus Musculus
+ MAPK3up-regulates img/indirect-activation.png transcriptional regulation SOX9 0.387
Identifier Residue Sequence Organism Cell Line
SIGNOR-209965 Homo sapiens
pmid sentence
Soluble pref-1 inhibits adipocyte differentiation through the activation of extracellular signal-regulated kinase/mitogen-activated protein kinase (erk/mapk) and the subsequent upregulation of sox9 expression.
Publications: 1 Organism: Homo Sapiens
+ PTPRBup-regulates img/direct-activation.png dephosphorylation MAPK3 0.439
Identifier Residue Sequence Organism Cell Line
SIGNOR-103165 Homo sapiens
pmid sentence
When cells are stimulated with various ligands such as growth factors, hormones, neurotransmitters, or tumor promoters, erk1/2 is activated through dualphosphorylation at the -ptepy-motif. Subsequently, p-erk1/2 translocates into the nucleus and phosphorylates elk-1, thereby acting as a transcription factor for cell proliferationthese data indicate that sa-p-erk1/2 might not only be regulated by mkp such as rvhr, but also by pp1 and ptp as well
Publications: 1 Organism: Homo Sapiens
+ DUSP6down-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.851
Identifier Residue Sequence Organism Cell Line
SIGNOR-103149 Homo sapiens
pmid sentence
P-erk1/2 proteins were efficiently dephosphorylated in vitro by protein phosphatases 1 and 2a (pp1/2a) and mapk phosphatase 3 (mkp3).
Publications: 1 Organism: Homo Sapiens
+ PPP2R5Cdown-regulates img/direct_inhibition.png binding MAPK3 0.404
Identifier Residue Sequence Organism Cell Line
SIGNOR-144328 Homo sapiens
pmid sentence
B56-containing pp2a dephosphorylate erk and their activity is controlled by the early gene iex-1 and erk
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MKNK2 0.504
Identifier Residue Sequence Organism Cell Line
SIGNOR-48355 Homo sapiens
pmid sentence
Erk and p38 phosphorylate mnk1 and mnk2, which stimulates their in vitro kinase activity.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates activity img/direct-activation.png phosphorylation MKNK1 0.564
Identifier Residue Sequence Organism Cell Line
SIGNOR-253012 in vitro
pmid sentence
These results indicate that MNK1 is a novel class of protein kinase that is activated through both the ERK and p38 MAP kinase signaling pathways
Publications: 1 Organism: In Vitro
+ PPP1CAdown-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.451
Identifier Residue Sequence Organism Cell Line
SIGNOR-103155 Homo sapiens
pmid sentence
P-erk1/2 proteins were efficiently dephosphorylated in vitro by protein phosphatases 1 and 2a (pp1/2a) and mapk phosphatase 3 (mkp3). the dual specificity phosphatases that specifically dephosphorylate and inactivate the p-erk1/2 are called mapk phosphatases
Publications: 1 Organism: Homo Sapiens
+ DUSP5down-regulates img/direct_inhibition.png dephosphorylation MAPK3 0.754
Identifier Residue Sequence Organism Cell Line
SIGNOR-67358 Homo sapiens
pmid sentence
Extracellular regulated kinases (erk) 1 and erk2 are authentic substrates for the dual-specificity protein-tyrosine phosphatase vhr. A novel role in down-regulating the erk pathway
Publications: 1 Organism: Homo Sapiens
+ MAP2K1up-regulates activity img/direct-activation.png phosphorylation MAPK3 0.745
Identifier Residue Sequence Organism Cell Line
SIGNOR-258994 Mus musculus
pmid sentence
Raf proteins have been shown to phosphorylate and activate MAPKKs (MAP kinase kinases) called MEKs (MAPK or ERK kinases) which in turn phosphorylate and activate MAPKs (MAP kinases) called ERKs
Publications: 1 Organism: Mus Musculus
+ MAPK3down-regulates activity img/direct_inhibition.png relocalization PPARG 0.405
Identifier Residue Sequence Organism Cell Line
SIGNOR-179407 Homo sapiens
pmid sentence
The genomic activity of ppargamma is modulated, in addition to ligand binding, by phosphorylation of a serine residue by mapks, such as extracellular signal-regulated protein kinases-1/2 (erk-1/2), or by nucleocytoplasmic compartmentalization through the erk activators mapk kinases-1/2 (mek-1/2). These mapks phosphorylate (in humans) ser 84 in the ppargamma1 and ser 114 in ppargamma2 isoform
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates img/direct-activation.png phosphorylation MYL1 0.29
Identifier Residue Sequence Organism Cell Line
SIGNOR-148002 Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell
pmid sentence
Activation of raf/mek/erk cascade can also result in the phosphorylation of the antiapoptotic mcl-1 protein and the pro-apoptotic bim protein.
Publications: 1 Organism: Homo Sapiens
+ MAPK3up-regulates quantity by expression img/indirect-activation.png transcriptional regulation SOX9 0.387
Identifier Residue Sequence Organism Cell Line
SIGNOR-165353 Homo sapiens
pmid sentence
Soluble pref-1 inhibits adipocyte differentiation through the activation of extracellular signal-regulated kinase/mitogen-activated protein kinase (erk/mapk) and the subsequent upregulation of sox9 expression.
Publications: 1 Organism: Homo Sapiens
a simple tooltip